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BACKGROUND Malvidin (alvidin-3-glucoside) is a polyphenol that belongs to the class of natural anthocyanin, which is abundantly found in red wines, colored fruits, and the skin of red grapes. Therefore, the current investigation was intended to evaluate the effect of malvidin against myocardial infarction induced by isoproterenol in the rats. MATERIAL AND METHODS The cardioprotective effects was assessed by determining the effect of malvidin on the activities of endogenous antioxidants - catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH) - and on the levels of lipid peroxidation and serum marker enzymes. The serum levels of IL-6 and TNF-α were also determined using an enzyme-linked immunosorbent assay (ELISA) kit. RESULTS The present study demonstrated a significant cardioprotective effect of malvidin by restoring the defensive activities of endogenous antioxidants - catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH) - and by reducing the levels of lipid peroxidation and serum marker enzymes lactate dehydrogenase (LD) and creatine kinase (CK). Malvidin significantly ameliorated the histopathological changes and impaired mitochondria in the cardiac necrosis stimulated with isoproterenol. Additionally, the results also demonstrated that nuclear translocation of Nrf-2 and subsequent HO-1 expression might be associated with nuclear factor kappa B (NF-κB) pathway activation. CONCLUSIONS Our findings suggest that malvidin exerts cardioprotective effects that might be due to possible strong antioxidant and anti-inflammatory activities. Therefore, this study provides the basis for the development of malvidin as a safe and effective treatment of myocardial infarction.
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Antocianinas/uso terapéutico , Infarto del Miocardio/tratamiento farmacológico , Infarto del Miocardio/prevención & control , Animales , Antocianinas/farmacología , Antioxidantes/farmacología , Catalasa/efectos de los fármacos , Catalasa/metabolismo , Ensayo de Inmunoadsorción Enzimática , Glutatión Peroxidasa/efectos de los fármacos , Glutatión Peroxidasa/metabolismo , Corazón/efectos de los fármacos , Isoproterenol/farmacología , Peroxidación de Lípido/efectos de los fármacos , Masculino , Infarto del Miocardio/metabolismo , Miocardio/patología , Fitoterapia , Extractos Vegetales/farmacología , Ratas , Ratas Wistar , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismoRESUMEN
BACKGROUND/AIMS: Myeloid-derived suppressor cells (MDSCs) are increased in inflammatory and autoimmune disorders. This study aims to evaluate the significance of MDSCs in dilated cardiomyopathy (DCM) patients. METHODS: In total, 42 newly hospitalized DCM patients and 39 healthy controls were enrolled in the study. The frequencies of circulating CD14+HLA-DR-/low MDSCs were determined by flow cytometry. Then, the functional properties of MDSCs in suppressing T cell proliferation and interferon-gamma (IFN-x03B3;) production were measured in a co-culture model. Then, mRNA expression levels of various important molecules in peripheral blood mononuclear cells were measured by real time polymerase chain reaction. Furthermore, correlation analyses between MDSC frequencies and cardiac function parameters were also performed. RESULTS: The frequencies of circulating CD14+HLA-DR-/low MDSCs were significantly elevated in DCM patients compared with healthy controls. It showed that MDSCs from DCM patients more effectively suppressed T cell proliferation and IFN-x03B3; production compared with those from healthy controls, which was partially mediated by arginase-1 (Arg-1). In addition, the correlation analysis suggested that MDSC frequencies were negatively correlated with left ventricular ejection fraction (LVEF), while positively with N-terminal pro-brain natriuretic peptide (NT-proBNP) in patients with DCM. CONCLUSIONS: Circulating activated MDSCs might play significant immunomodulatory roles in the pathogenesis of DCM.
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Cardiomiopatía Dilatada/patología , Inflamación/patología , Células Supresoras de Origen Mieloide/patología , Cardiomiopatía Dilatada/complicaciones , Cardiomiopatía Dilatada/inmunología , Femenino , Antígenos HLA-DR/análisis , Antígenos HLA-DR/inmunología , Humanos , Tolerancia Inmunológica , Inflamación/complicaciones , Inflamación/inmunología , Receptores de Lipopolisacáridos/análisis , Receptores de Lipopolisacáridos/inmunología , Masculino , Persona de Mediana Edad , Células Supresoras de Origen Mieloide/inmunología , Miocardio/inmunología , Miocardio/patología , Linfocitos T/inmunología , Linfocitos T/patologíaRESUMEN
AIM: The aim of this study was to explore whether the circulating frequency and function of myeloid-derived suppressor cells (MDSCs) are altered in patients with acute coronary syndrome (ACS). METHODS: The frequency of MDSCs in peripheral blood was determined by flow cytometry, and mRNA expression in purified MDSCs was analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). The suppressive function of MDSCs isolated from different groups was also determined. The plasma levels of certain cytokines were determined using Bio-Plex Pro™ Human Cytokine Assays. RESULTS: The frequency of circulating CD14(+)HLA-DR(-/low) MDSCs; arginase-1 (Arg-1) expression; and plasma levels of interleukin (IL)-1ß, IL-6, tumor necrosis factor (TNF)-α, and IL-33 were markedly increased in ACS patients compared to stable angina (SA) or control patients. Furthermore, MDSCs from ACS patients were more potent suppressors of T-cell proliferation and IFN-γ production than those from the SA or control groups at ratios of 1:4 and 1:2; this effect was partially mediated by Arg-1. In addition, the frequency of MDSCs was positively correlated with plasma levels of IL-6, IL-33, and TNF-α. CONCLUSIONS: We observed an increased frequency and suppressive function of MDSCs in ACS patients, a result that may provide insights into the mechanisms involved in ACS.
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Síndrome Coronario Agudo/patología , Células Mieloides/metabolismo , Síndrome Coronario Agudo/metabolismo , Angina Estable/metabolismo , Angina Estable/patología , Arginasa/genética , Arginasa/metabolismo , Proliferación Celular , Células Cultivadas , Electrocardiografía , Femenino , Antígenos HLA-DR/metabolismo , Humanos , Interferón gamma/metabolismo , Interleucina-1beta/sangre , Interleucina-33 , Interleucina-6/sangre , Interleucinas/sangre , Leucocitos Mononucleares/citología , Receptores de Lipopolisacáridos/metabolismo , Masculino , Persona de Mediana Edad , Células Mieloides/citología , ARN Mensajero/metabolismo , Linfocitos T/citología , Linfocitos T/inmunología , Linfocitos T/metabolismo , Factor de Necrosis Tumoral alfa/sangreRESUMEN
OBJECTIVE: To investigate the role of CD4(+)CD25(+) T cells (Tregs) in protecting fine particulate matter (PM-) induced inflammatory responses, and its potential mechanisms. METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with graded concentrations (2, 5, 10, 20, and 40 µg/cm(2)) of suspension of fine particles for 24h. For coculture experiment, HUVECs were incubated alone, with CD4(+)CD25(-) T cells (Teff), or with Tregs in the presence of anti-CD3 monoclonal antibodies for 48 hours, and then were stimulated with or without suspension of fine particles for 24 hours. The expression of adhesion molecules and inflammatory cytokines was examined. RESULTS: Adhesion molecules, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and inflammatory cytokines, such as interleukin (IL-) 6 and IL-8, were increased in a concentration-dependent manner. Moreover, the adhesion of human acute monocytic leukemia cells (THP-1) to endothelial cells was increased and NF- κ B activity was upregulated in HUVECs after treatment with fine particles. However, after Tregs treatment, fine particles-induced inflammatory responses and NF- κ B activation were significantly alleviated. Transwell experiments showed that Treg-mediated suppression of HUVECs inflammatory responses impaired by fine particles required cell contact and soluble factors. CONCLUSIONS: Tregs could attenuate fine particles-induced inflammatory responses and NF- κ B activation in HUVECs.
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Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/inmunología , Inflamación/inmunología , Inflamación/metabolismo , Material Particulado/toxicidad , Linfocitos T Reguladores/metabolismo , Humanos , Molécula 1 de Adhesión Intercelular/sangre , Interleucina-8/sangre , FN-kappa B/sangre , Molécula 1 de Adhesión Celular Vascular/sangreRESUMEN
Background: Atherosclerosis is an inflammatory disease involving activation of adaptive and innate immune responses to antigens, including oxidized low-density lipoprotein (oxLDL) and phosphorylcholine (PC). Dendritic cells (DCs), which are antigen-presenting cells that activate T cells, are present in atherosclerotic lesions and are activated in immune organs. However, the mechanism by which PC promotes atherosclerosis is unclear. MethodsâandâResults: To evaluate whether PC promotes atherosclerosis via DCs, 2×105 DCs activated by PC-keyhole limpet hemocyanin (DCs+PC-KLH) were injected into ApoE-/- mice and the features of the plaques and the effects of the DCs on cellular and humoral immunity against PC-KLH were determined. Mice injected with DCs+PC-KLH had significantly larger atherosclerotic lesions than controls, with increased inflammation in the lesions and plaque instability. Furthermore, DCs+PC-KLH were characterized using flow cytometry after coculture of bone marrow-derived DCs and naïve T cells. DCs+PC-KLH showed an inflammatory phenotype, with increased CD86, CD40, and major histocompatibility complex Class II molecules (MHC-II), which promoted PC-specific T helper (Th) 1 and Th17 cell differentiation in vivo and in vitro. Moreover, 2 weeks after the administration of DCs+PC-KLH to mice, these mice produced PC- and oxLDL-specific IgG2a, compared with no production in the controls. Conclusions: These findings suggest that DCs presenting PC promote specific immunity to PC, increase lesion inflammation, and accelerate atherosclerosis, which may explain how PC promotes atherosclerosis.
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Many studies have suggested that VSMC (vascular smooth muscle cell) apoptosis plays a key role in destabilization and rupture of atherosclerotic plaques. Therefore protection for VSMCs from apoptosis is a promising approach to stabilize 'vulnerable' lesions. However, the mechanisms as to why VSMCs in the fibrous cap often appear as profilerated in early stages, but turn apoptotic in advanced stages, are still unknown. In the present study, using RNAi (RNA interference) technology and a CaN (calcineurin) antagonist, the correlation between CaN and RANTES (regulated upon activation, normal T-cell expressed and secreted) in cultured rat apoptotic VSMCs stimulated by IFNgamma (interferon gamma; 20 ng/ml) and CD40L (CD40 ligand; 100 ng/ml) was investigated. RANTES released from VSMCs in each group was measured by ELISA and its mRNA in VSMCs was determined by RT (reverse transcription)-PCR. The total activity and expression of CaN in VSMCs were detected by the zymochemistry method and Western blot analysis respectively. From the results of the present study it can be hypothesized that an elevated CaN concentration in endochylema, by the CD40-CD40L signal pathway, induces VSMC apoptosis accomplished by the overexpression of RANTES. Therefore RANTES is a potential target for treating vulnerable atherosclerotic plaques owing to its crucial downstream regulating role in CaN-dependent VSMC apoptosis.
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Apoptosis , Ligando de CD40/farmacología , Calcineurina/inmunología , Quimiocina CCL5/inmunología , Interferón gamma/farmacología , Músculo Liso Vascular/citología , Miocitos del Músculo Liso , Animales , Apoptosis/efectos de los fármacos , Apoptosis/inmunología , Ligando de CD40/inmunología , Calcineurina/genética , Inhibidores de la Calcineurina , Células Cultivadas , Quimiocina CCL5/genética , Citocinas/genética , Citocinas/inmunología , Interferón gamma/inmunología , Masculino , Miocitos del Músculo Liso/citología , Miocitos del Músculo Liso/efectos de los fármacos , Miocitos del Músculo Liso/inmunología , Interferencia de ARN , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Transducción de Señal/efectos de los fármacosRESUMEN
Oxidative stress and subsequent cardiac myocyte apoptosis play central roles in the initiation and progression of myocardial ischemia-reperfusion (I/R) injury. Homeobox transcript antisense intergenic RNA (Hotair) was previously implicated in various heart diseases, yet its role in myocardial I/R injury has not been clearly demonstrated. Mice with cardiac-restricted knockdown or overexpression of Hotair were exposed to I/R surgery. H9c2 cells were cultured and subjected to hypoxia/reoxygenation (H/R) stimulation to further verify the role and underlying mechanisms of Hotair in vitro. Histological examination, molecular detection, and functional parameters were determined in vivo and in vitro. In response to I/R or H/R treatment, Hotair expression was increased in a bromodomain-containing protein 4-dependent manner. Cardiac-restricted knockdown of Hotair exacerbated, whereas Hotair overexpression prevented I/R-induced oxidative stress, cardiac myocyte apoptosis, and cardiac dysfunction. Mechanistically, we observed that Hotair exerted its beneficial effects via activating AMP-activated protein kinase alpha (AMPKα). Further detection revealed that Hotair activated AMPKα through regulating the enhancer of zeste homolog 2/microRNA-451/calcium-binding protein 39 (EZH2/miR-451/Cab39) axis. We provide the evidence that endogenous lncRNA Hotair is an essential negative regulator for oxidative stress and cardiac myocyte apoptosis in myocardial I/R injury, which is dependent on AMPKα activation via the EZH2/miR-451/Cab39 axis.
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Daño por Reperfusión Miocárdica/genética , Miocitos Cardíacos/metabolismo , Estrés Oxidativo/genética , ARN Largo no Codificante/metabolismo , Animales , Apoptosis , Masculino , RatonesRESUMEN
AIM: To investigate the correlation between the levels of serum IFN-γ and RANTES (regulated upon on activation on normal T cell expressed and secreted) and rabbit atherosclerotic plaque stability. METHODS: 30 male New Zealand rabbits were randomly divided into three groups: control group, stable atherosclerotic plaque group(AS group) and vulnerable atherosclerotic plaque group(VAP group), with 10 rabbits in a group. The control group was given normal diet while the AS group and the VAP group were given high-fat diet for 12 weeks. According to literature method pharmacological triggers inducing the vulnerable atherosclerotic plaque formation in the VAP group. Fasting blood was extracted from ear medium artery about 2 mL at week-0 and the 12th week, serum total cholesterol, triglyceride and high density lipoprotein cholesterol were detected by enzymatic method, and low density lipoprotein cholesterol was calculated by Friedwald formula. All animals were executed after they were fed by injected excessive Phenobarbital sodium at ear vein for 12 weeks. Before executed, ear medium artery blood was taken to detect the levels of serum IFN-γ and RANTES by ELISA.The corrected plaque area and vulnerability index were calculated by Masson staining and its correlation coefficient with inflammatory factors were measured. RESULTS: The level of serum IFN-γ at the AS group and the VAP group, compared with the control group, was significantly increased(all P<0.01), at the same time, the level of serum IFN-γ in the VAP group was significantly higher than in the AS group(P<0.01). About the level of serum RANTES there was no obvious difference when the AS group was compared with the control group(P>0.05). The level of serum RANTES in the VAP group was higher than in the AS group and the control group(all P<0.01). Regarding corrected plaque area and vulnerability Index, the VAP group was significantly higher than the AS group (P<0.01). In the AS group and the VAP group, the serum concentrations of IFN-γand RANTES exhibited a positive correlation with corrected plaque area and vulnerability Index (P<0.05). CONCLUSION: IFN-γ and RANTES may be inflammatory marker about atherosclerotic vulnerable plaque, and their serum concentration can be used to evaluate the atherosclerotic plaque instability.
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Quimiocina CCL5/sangre , Interferón gamma/sangre , Placa Aterosclerótica/metabolismo , Placa Aterosclerótica/patología , Animales , Quimiocina CCL5/metabolismo , Colesterol/sangre , Mediadores de Inflamación/metabolismo , Interferón gamma/metabolismo , Masculino , ConejosRESUMEN
BACKGROUND AND AIMS: There is an imbalance between Th1 and Th2 in the development and progression of atherosclerosis and in patients with acute coronary syndrome (ACS) including acute myocardial infarction (AMI) and unstable angina. T helper cell type 3 (Th3), which primarily secretes transforming growth factor beta-1 (TGF-beta1), has been shown to inhibit both Th1 and Th2 cells. The present study was designed to investigate whether Th3 cells are involved in plaque destabilization and the onset of ACS. METHODS: Ninety one patients who underwent diagnostic catheterization were classified into four groups (AMI group, unstable angina group, stable angina group and chest pain syndrome group). The cell frequencies of Th1, Th2 and Th3 were detected using flow cytometry, and the concentrations of their related cytokines IFN-gamma, IL-4 and TGF-beta1 were studied by ELISA. RESULTS: Apart from the imbalance between Th1 and Th2, results revealed a significant decrease in peripheral Th3 number and levels of TGF-beta1 in patients with ACS as compared with those in patients with stable angina and chest pain syndrome (p<0.01). CONCLUSIONS: Downregulation of Th3 cells in patients with ACS may play a potential role in plaque destabilization and the onset of ACS.