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This article proposes a framework for understanding the macro-scale organization of anatomical pathways in the mammalian brain. The architecture supports flexible behavioral decisions across a spectrum of spatio-temporal scales. The proposal emphasizes the combinatorial, reciprocal, and reentrant connectivity-called CRR neuroarchitecture-between cortical, BG, thalamic, amygdala, hypothalamic, and brainstem circuits. Thalamic nuclei, especially midline/intralaminar nuclei, are proposed to act as hubs routing the flow of signals between noncortical areas and pFC. The hypothalamus also participates in multiregion circuits via its connections with cortex and thalamus. At slower timescales, long-range behaviors integrate signals across levels of the neuroaxis. At fast timescales, parallel engagement of pathways allows urgent behaviors while retaining flexibility. Overall, the proposed architecture enables context-dependent, adaptive behaviors spanning proximate to distant spatio-temporal scales. The framework promotes an integrative perspective and a distributed, heterarchical view of brain function.
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Animals face behavioral problems that can be conceptualized in terms of a gradient of spatial and temporal proximity. I propose that solving close-proximity behavioral problems involves integrating disparate types of information in complex and flexible ways. In this framework, the midbrain periaqueductal gray (PAG) is understood as a key region involved in close-proximity motivated cognition. Anatomically, the PAG has access to signals across the neuroaxis via extensive connectivity with cortex, subcortex, and brainstem. However, the flow of signals is not unidirectional, as the PAG projects to the cortex directly, and further ascending signal flow is attained via the midline thalamus. Overall, the anatomical organization of the PAG allows is to be a critical hub engaged in cognition "here and now".
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In the past decades, functional MRI research has investigated mental states and their brain bases in largely static fashion based on evoked responses during blocked and event-related designs. Despite some progress in naturalistic designs, our understanding of threat processing remains largely limited to those obtained with standard paradigms. In the present paper, we applied Switching Linear Dynamical Systems to uncover the dynamics of threat processing during a continuous threat-of-shock paradigm. Importantly, unlike studies in systems neuroscience that frequently assume that systems are decoupled from external inputs, we characterized both endogenous and exogenous contributions to dynamics. First, we demonstrated that the SLDS model learned the regularities of the experimental paradigm, such that states and state transitions estimated from fMRI time series data from 85 ROIs reflected both the proximity of the circles and their direction (approach vs. retreat). After establishing that the model captured key properties of threat-related processing, we characterized the dynamics of the states and their transitions. The results revealed that threat processing can profitably be viewed in terms of dynamic multivariate patterns whose trajectories are a combination of intrinsic and extrinsic factors that jointly determine how the brain temporally evolves during dynamic threat. We propose that viewing threat processing through the lens of dynamical systems offers important avenues to uncover properties of the dynamics of threat that are not unveiled with standard experimental designs and analyses.
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Large-scale functional networks have been characterized in both rodent and human brains, typically by analyzing fMRI-BOLD signals. However, the relationship between fMRI-BOLD and underlying neural activity is complex and incompletely understood, which poses challenges to interpreting network organization obtained using this technique. Additionally, most work has assumed a disjoint functional network organization (i.e., brain regions belong to one and only one network). Here, we employ wide-field Ca2+ imaging simultaneously with fMRI-BOLD in mice expressing GCaMP6f in excitatory neurons. We determine cortical networks discovered by each modality using a mixed-membership algorithm to test the hypothesis that functional networks exhibit overlapping organization. We find that there is considerable network overlap (both modalities) in addition to disjoint organization. Our results show that multiple BOLD networks are detected via Ca2+ signals, and networks determined by low-frequency Ca2+ signals are only modestly more similar to BOLD networks. In addition, the principal gradient of functional connectivity is nearly identical for BOLD and Ca2+ signals. Despite similarities, important differences are also detected across modalities, such as in measures of functional connectivity strength and diversity. In conclusion, Ca2+ imaging uncovers overlapping functional cortical organization in the mouse that reflects several, but not all, properties observed with fMRI-BOLD signals.
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Mapeo Encefálico , Encéfalo , Humanos , Ratones , Animales , Encéfalo/diagnóstico por imagen , Encéfalo/fisiología , Mapeo Encefálico/métodos , Imagen por Resonancia Magnética/métodos , Algoritmos , NeuronasRESUMEN
Coffee processing generates large amounts of residues of which a portion still has bioactive properties due to their richness in phenolic compounds. This study aimed to obtain a coffee husks extract (CHE) and to encapsulate it (ECHE) with polyvinylpyrrolidone using a one-step procedure of solid dispersion. The extraction and encapsulation yields were 9.1% and 92%, respectively. Thermal analyses revealed that the encapsulation increased the thermal stability of CHE and dynamic light scattering analyses showed a bimodal distribution of size with 81% of the ECHE particles measuring approximately 711 nm. Trigonelline and caffeine were the main alkaloids and quercetin the main phenolic compound in CHE, and the encapsulation tripled quercetin extraction. The total phenolics content and the antioxidant activity of ECHE, assayed with three different procedures, were higher than those of CHE. The antioxidant activity and the bioaccessibility of the phenolic compounds of ECHE were also higher than those of CHE following simulated gastrointestinal digestion (SGID). Both CHE and ECHE were not toxic against Alliumcepa cells and showed similar capacities for inhibiting the pancreatic α-amylase in vitro. After SGID, however, ECHE became a 1.9-times stronger inhibitor of the α-amylase activity in vitro (IC50 = 8.5 mg/mL) when compared to CHE. Kinetic analysis revealed a non-competitive mechanism of inhibition and in silico docking simulation suggests that quercetin could be contributing significantly to the inhibitory action of both ECHE and CHE. In addition, ECHE (400 mg/kg) was able to delay by 50% the increases of blood glucose in vivo after oral administration of starch to rats. This finding shows that ECHE may be a candidate ingredient in dietary supplements used as an adjuvant for the treatment of diabetes.
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Antioxidantes , Coffea , Ratas , Animales , Antioxidantes/análisis , Quercetina , Povidona , Coffea/química , CinéticaRESUMEN
Abstract The use and occupation of land by human population substantially influence environmental variables and fish assemblage in streams. However, there is little knowledge on how these changes affect the ecomorphological structure of fish assemblage in mesohabitats. Therefore, we aim to assess whether the land-use types affect the ecomorphological structure of fish assemblage in distinct mesohabitats. Environmental and ichthyofaunistic data were collected in three mesohabitats (rifles, runs, and pools) of five rural and five urban streams. Twenty-one ecomorphological indices were obtained from the mean of linear morphological measurements and areas of the fishes. Subsequently, the Euclidean distance was calculated, based on the ecomorphological indices, between each pair of species, to measure the ecomorphological distances for the mesohabitats of the rural and urban streams. The results show that the urban environment is more harmful to streams than the rural one, due to changes in the environmental variables and decrease in species richness. The main environmental changes found in urban streams were the decrease in canopy cover by riparian vegetation and dissolved oxygen, and the increase in electrical conductivity and bed silting. Also, there was a significant decrease in the morphological similarity between fish species in the mesohabitats of urban streams compared to rural ones. Therefore, we can conclude that the urban environment leads to the loss of morphologically similar fish species in the mesohabitats, with only a few functionally distinct species remaining.
Resumo O uso e ocupação do solo pela população humana influencia substancialmente as variáveis ambientais e a assembleia de peixes em riachos. No entanto, há pouco conhecimento de como estas alterações afeta a estrutura ecomorfológica da assembleia de peixes em mesohábitats. Portanto, objetiva-se avaliar se o tipo do uso do solo afeta a estrutura ecomorfológica da assembleia de peixes nos distintos mesohábitats. Dados ambientais e ictiofaunísticos foram coletados em três mesohabitats (corredeiras, rápidos e remansos) de cinco riachos rurais e cinco urbanos. Vinte e um índices ecomorfológicos foram obtidos a partir das médias das medidas morfológicas lineares e áreas dos peixes. Posteriormente, a distância Euclidiana foi calculada, baseada nos índices ecomorfológicos, entre cada par de espécies, afim de mensurar as distâncias ecomorfológicas para os mesohábitats dos riachos rurais e urbanos. Os resultados mostram que o ambiente urbano é mais danoso aos riachos do que o rural, devido a diminuição da riqueza de espécies e as alterações nas variáveis ambientais. As principais mudanças ambientais encontradas em riachos urbanos foram a diminuição da cobertura do dossel pela vegetação ripária e do oxigênio dissolvido e o aumento da condutividade elétrica e do assoreamento do leito. Além disso, houve uma diminuição significativa da similaridade morfológica entre as espécies de peixes nos mesohábitats de riachos urbanos em comparação com os rurais. Portanto, podemos concluir que o ambiente urbano leva à perda de espécies de peixes morfologicamente similares nos mesohábitats, restando apenas algumas espécies funcionalmente distintas.
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Plant tissue culture has emerged as an important tool to produce bioactive compounds from various plant species, including the sustainable production of limonoids that are receiving considerable attention due to the benefits associated with human health such as anticancer activities. The purpose of the present study was to evaluate the capacity of limonoids aglycone production from callus culture from sweet orange cv. Pera (Citrus sinensis) seeds and identify the compounds produced in this cell line. Callus induction occurred in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic (2,4-D), malt extract, agar and coconut water. For the analysis and identification of the limonoids, CG-MS-EI ion-positive mode and UPLC-QTOF-ESI were used operating in positive and negative mode. An intense peak corresponding to limonin appeared in the callus extracts at a retention time of 58.1 min. in CG-MS-EI and four major limonoids aglycone by positive ion mode UPLC-QTOF-ESI: limonin, nomilin, deacetylnomilin, and nomilinic acid. The culture medium was efficient at the bioproduction of limonoids aglycone in callus cultures of C. sinensis seeds. Therefore, data obtained from UPLC-QTOF-ESI proved its importance at identifying new compounds that benefit human health, and may assist future work in the identification of known or new limonoids in Citrus species and related genera.
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Biotecnología , Citrus sinensis/química , Fitoquímicos , Limoninas/clasificaciónRESUMEN
No presente estudo investigamos se estímulos emocionais distrativos são capazes de interferir na realização de tarefas nas quais é exigido um alto engajamento da atenção. Para tanto foi realizado um teste de discriminação de orientação entre duas barras apresentadas bilateralmente na periferia do campo visual (julgamento igual ou diferente). O grau de dificuldade da tarefa dependia da diferença de orientação das mesmas. Simultaneamente era apresentada uma figura central (neutra ou negativa) entre as barras. Foi observado que as figuras negativas interferiram no desempenho dos voluntários mesmo na tarefa mais difícil, que exigia maior engajamento atencional. Além disto, quando o reconhecimento posterior destas figuras era testado, figuras negativas eram mais lembradas do que neutras. Estes dados sugerem que estímulos emocionais são privilegiados na disputa por recursos de atenção. É possível que esse privilégio ocorra devido a relevância destes estímulos, sendo dificilmente ignorados
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Atención , Emociones , Tiempo de ReacciónRESUMEN
A emoçäo pode ser funcionalmente considerada como uma disposiçäo à açäo que prepara o organismo para comportamentos relacionados à aproximaçäo e esquiva. Para preparar uma saída motora apropriada, o organismo tem que ser eficiente na codificaçäo de estímulos relevantes. Neste trabalho, apresentamos evidências a partir de estudos de neuroimagem que revelam que a visualizaçäo de imagens emocionais promove uma maior ativaçäo do córtex visual do que a observaçäo de figuras neutras. Além desta facilitaçäo do processamento sensorial, os estímulos emocionais desencadeiam reaçöes somáticas e vegetativas. Registros da dinâmica postural e da freqüência cardíaca enquanto voluntários assistiam a um bloco de figuras desagradáveis revelou uma reduçäo significativa na oscilaçäo corporal e bradicardia. Uma investigaçäo paralela mostrou que o tempo de reaçäo também lentifica após a visualizaçäo de figuras negativas. Este conjunto de respostas - imobilidade, bradicardia e tempo de reaçäo mais lento - pode refletir o engajamento do sistema defensivo, similar às reaçöes defensivas desencadeadas em ambiente natural por estímulos ameaçadores distantes. Em resumo, o sistema afetivo influencia um nível precoce de codificaçäo sensorial e a saída motora favorecendo, portanto, disposiçöes para as açöes apropriadas