Optimization of pig slurry application to heavy metal polluted soils monitoring nitrification processes.

Nitrification is often negatively affected by heavy metal pollution in soils, this limiting land revegetation. Thus, the potential use of pig slurry as a nitrogen-rich organic amendment in different heavy metal contaminated soils has been evaluated; this also being a way of recycling this waste. In order to identify the factors affecting nitrification processes in heavy metal polluted soils (soil pH, heavy metal solubility and the N source), incubation experiments were run using two polluted soils with different pH values (5.0 and 7.1) and a non-contaminated soil (pH 8.2). Ammonium was added as pig slurry or as ammonium sulphate for comparison (both added at 150 mg NH(4)(+)-N kg(-1) of soil). Pig slurry provoked higher nitrification rates and N-immobilisation than ammonium sulphate, especially in the neutral-polluted soil, reflecting an improvement of the microbial activity in the soil. The microbial immobilisation of N led to an inverse relationship between the amount of N added and nitrate conversion in the neutral-polluted soil and in the non-contaminated soil amended with different pig slurry dosages (75, 150 and 225mg NH(4)(+)-N kg(-1) of soil). Low rates of nitrification and N-immobilisation were found in the acidic soil. Pig slurry addition to metal polluted soils enhanced soil nitrification, especially when metals were in low-solubility forms.

A plant genetically modified that accumulates Pb is especially promising for phytoremediation.

From a number of wild plant species growing on soils highly contaminated by heavy metals in Eastern Spain, Nicotiana glauca R. Graham (shrub tobacco) was selected for biotechnological modification, because it showed the most appropriate properties for phytoremediation. This plant has a wide geographic distribution, is fast-growing with a high biomass, and is repulsive to herbivores. Following Agrobacterium mediated transformation, the induction and overexpression of a wheat gene encoding phytochelatin synthase (TaPCS1) in this particular plant greatly increased its tolerance to metals such as Pb and Cd, developing seedling roots 160% longer than wild type plants. In addition, seedlings of transformed plants grown in mining soils containing high levels of Pb (1572 ppm) accumulated double concentration of this heavy metal than wild type. These results indicate that the transformed N. glauca represents a highly promising new tool for use in phytoremediation efforts.