Water-soluble peptide-coated nanoparticles: control of the helix structure and enhanced differential binding to immune cells.

The stabilizing action of C(α)-tetrasubstituted α-amino acids inserted into a sequence of short peptides allowed for the first time the preparation of water-soluble nanoparticles of different materials coated with a helix-structured undecapeptide. This peptide coating strongly favors nanoparticle uptake by human immune system cells.

The cellular uptake of meta-tetra(hydroxyphenyl)chlorin entrapped in organically modified silica nanoparticles is mediated by serum proteins.

Nanosized objects made of various materials are gaining increasing attention as promising vehicles for the delivery of therapeutic and diagnostic agents for cancer. Photodynamic therapy (PDT) appears to offer a very attractive opportunity to implement drug delivery systems since no release of the sensitizer is needed to obtain the therapeutic effect and the design of the nanovehicle should be much easier. The aim of our study was to investigate the use of organic-modified silica nanoparticles (NPs) for the delivery of the second-generation photosensitizer meta-tetra(hydroxyphenyl)chlorin (mTHPC) to cancer cells in vitro. mTHPC was entrapped in NPs (approximately 33 nm diameter) in a monomeric form which produced singlet oxygen with a high efficiency. In aqueous media with high salt concentrations, the NPs underwent aggregation and precipitation but their stability could be preserved in the presence of foetal bovine serum. The cellular uptake, localization and phototoxic activity of mTHPC was determined comparatively in human oesophageal cancer cells after its delivery by the NPs and the standard solvent ethanol/poly(ethylene glycol) 400/water (20:30:50, by vol). The NP formulation reduced the cellular uptake of mTHPC by about 50% in comparison to standard solvent while it did not affect the concentration-dependent photokilling activity of mTHPC and its intracellular localization. Fluorescence resonance energy transfer measurements, using NPs with mTHPC physically entrapped and a cyanine covalently linked, and ultracentrifugation experiments indicated that mTHPC is transferred from NPs to serum proteins when present in the medium. However, the coating of the NP surface with poly(ethylene glycol) largely prevented the transfer to proteins. In conclusion, mTHPC is rapidly transferred from the uncoated nanoparticles to the serum proteins and then internalized by the cells as a protein complex, irrespective of its modality of delivery.

Vial sonication and ultrasonic immersion probe sonication to generate stable dispersions of multiwall carbon nanotubes for physico-chemical characterization and biological testing.

Nanotechnology is considered to be a key enabling technology and in recent years there has been much growth in the use of nanostructured materials in industrial applications and in consumer products. It is, therefore, important that prior to being commercialized in consumer products, engineered nanomaterials are subjected to a thorough physico-chemical characterization as part of broader risk assessment to evaluate their possible effects on human health and the environment. The proper dispersion of nanomaterials sourced as powders becomes a first crucial step in the characterization process. This paper focuses on the dispersion of multiwall carbon nanotubes - often hydrophobic and tangled - since it may be challenging to re-disperse them effectively in aqueous media prior to characterization. A comparison has been made of non-contact vial sonication and immersion probe sonication using tannic acid as a dispersant. Transmission electron microscopy and UV-Vis spectroscopy were the techniques used to evaluate the dispersions. We used High Content Imaging and Colony Forming Efficiency to perform in vitro cytotoxicity studies on Human Alveolar Epithelial cells. It was found that both sonication treatments produce equivalent stable dispersions. No cytotoxic effects from MWCNTs were observed although some toxicity was observed and attributed to excess of the tannic acid dispersant.

Review of micro- and nanoplastic contamination in the food chain.

Whereas the dramatic environmental impact of plastic waste rightfully receives considerable attention by scientists, policy makers and public in general, the human health impact of micro- and nanoplastics contamination of our food and beverages remains largely unknown. Indeed, most studies aim at understanding the environmental impact rather than the human health impact of a possible exposure to micro- and nanoplastics. In addition, these papers generally lack a methodological, standardised approach. Furthermore, some studies focus on the damage to and contamination level of animal species collected from the wild environment, and others investigate the rate and biology of microplastic uptake of animals fed with microplastics in laboratory. This review aims at understanding human exposure. Since there is, with few exceptions, no evidence available on the presence of micro- and nanoplastics in a normal diet, this study takes an indirect approach and analyses peer-reviewed publications since 2010 that document the presence of micro- and nanoplastics in those animals (more than 200 species) and food products that are part of the human food chain and that may thus contribute directly or indirectly to the uptake of micro- and nanoplastics via the human diet. It also addresses the question of the definitions, the methodologies and the quality criteria applied to obtain the reported results. This review suggests that, beyond a few estimations and comparisons, precise data to assess the exact exposure of humans to micro- and nanoplastics through their diet cannot be produced until standardised methods and definitions are available.

Surface binding vs. sequestration; the uptake of benzohydroxamic acid at iron(iii) oxide surfaces.

Benzohydroxamic acid is shown to be an unexpectedly good ligand for iron(iii) oxides, favouring surface attachment to the formation of trisbenzohydroxamato complexes, which are known to have very high thermodynamic stability in solution.