[Protective effect of ophiopogonin D against isoproterenol-induced cardiomyocyte injury and targets].

This study aims to unveil the effect of ophiopogonin D(OPD) on isoproterenol(ISO)-induced apoptosis of rat cardiomyocytes and the possible targets, which is expected to provide clues for further research on the myocardial protection of ophiopogonins. Cell count kit-8(CCK-8) assay was used to detect viability of cells treated with OPD and ISO, Western blot to examine the effect of OPD and ISO on the expression of endoplasmic reticulum stress-related Bip, Bax, Perk, ATF4, caspase-12, and CHOP, flow cytometry to determine cell apoptosis rate, and Hoechst 33258 and Tunel staining to observe cell apoptosis and morphological changes. In addition, the probe for calcium ion-specific detection was employed to investigate calcium ion release from the endoplasmic reticulum, and OPD-bond epoxy-activated agarose solid-phase microspheres were prepared and used as affinity matrix to capture OPD-binding target proteins in H9 c2 cell lysate. For the target proteins of OPD identified by high-resolution mass spectrometry, the related signal pathways were enriched and the potential targets of OPD against cardiomyocyte injury were discussed. The experimental result showed that 10 µmol·L~(-1) ISO can significantly induce the expression of endoplasmic reticulum stress-related proteins and promote cell apoptosis. Different concentration of OPD can prevent the damage of myocardial cells caused by ISO. According to mass spectrometry results, 19 proteins, including Fam129 a and Pdia6, were involved in multiple signaling pathways such as the unfolded protein reaction bound by the ERN1 sensor, tricarboxylic acid cycle, and Nrf2 signal transduction pathway. The above results indicate that OPD protects cardiomyocytes by regulating multiple signaling pathways of target proteins and affecting cell cycle progression.

Induction of Non-Canonical Ferroptosis by Targeting Clusters Suppresses Glioblastoma.

Glioblastoma multiforme (GBM) is the most aggressive brain tumor. There is a pressing need to develop novel treatment strategies due to the poor targeting effect of current therapeutics. Here, a gold cluster coated with optimized GBM-targeting peptide is engineered, namely NA. NA can efficiently target GBM both in vitro and in vivo. Interestingly, the uptake of NA significantly sensitizes GBM cells to ferroptosis, a form of programmed cell death that can bypass the tumor resistance to apoptosis. This effect is exerted through regulating the HO-1-dependent iron ion metabolism, which is the non-canonical pathway of ferroptosis. The combined treatment of a ferroptosis inducer and NA profoundly inhibited tumor growth in both the GBM spheroid model and a syngeneic mouse model with enhanced ferroptosis levels and excellent biosafety. Importantly, the infiltration of tumoricidal lymphocytes is also significantly increased within tumor. Therefore, NA presents a potential novel nanomaterial-based strategy for GBM treatment.

Targeted Gold Nanoclusters for Synergistic High-Risk Neuroblastoma Therapy through Noncanonical Ferroptosis.

Children with extracranial high-risk neuroblastoma (NB) have a poor prognosis due to resistance against apoptosis. Recently, ferroptosis, another form of programmed cell death, has been tested in clinical trials for high-risk NB; however, drug resistance and side effects have also been observed. Here, we find that the gold element in gold nanoclusters can significantly affect iron metabolism and sensitize high-risk NB cells to ferroptosis. Accordingly, we developed a gold nanocluster conjugated with a modified NB-targeting peptide. This gold nanocluster, namely, NANT, shows excellent NB targeting efficiency and dramatically promotes ferroptosis. Surprisingly, this effect is exerted by elevating the noncanonical ferroptosis pathway, which is dependent on heme oxygenase-1-regulated Fe(II) accumulation. Furthermore, NANT dramatically inhibits the growth of high-risk NB in both tumor spheroid and xenograft models by promoting noncanonical ferroptosis evidenced by enhanced intratumoral Fe(II) and heme oxygenase-1. Importantly, this strategy shows excellent cardiosafety, offering a promising strategy to overcome ferroptosis resistance for the efficient and safe treatment of children with high-risk neuroblastoma.

Emodin-induced hepatotoxicity is enhanced by 3-methylcholanthrene through activating aryl hydrocarbon receptor and inducing CYP1A1 in vitro and in vivo.

BACKGROUND & AIMS: Polygonum multiflorum Thunb. (PMT) is the most common traditional Chinese medicine used to treat multiple diseases, and the hepatotoxicity caused by PMT has made great concern around world. Recent results showed that emodin is the potential toxic components of PMT, but the molecular mechanisms of emodin on liver toxicity remain to be elucidated. METHODS: Evaluation of parent- and metabolite-induced cytotoxicity in emodin were compared in L02 cells and mouse model from the perspective of drug metabolizing enzymes. The effect and mechanism of emodin-induced hepatotoxicity were analyzed using electrophoretic mobility shift, promoter reporter, and high content screening. RESULTS: We showed that emodin treatment (360 mg/kg in mice, 50 µM in L02 cells) induced hepatotoxicity and enhanced reactive oxidative stress (ROS) level. Importantly, emodin-induced ROS accumulation and hepatotoxicity were attenuated in the condition of CH223191, a selective inhibitor of aryl hydrocarbon receptor (AhR), and aggravated by 3-methylcholanthrene, a selective activator of AhR. Interestingly, we performed the study on ROS mediated ER stress and mitochondrial dysfunction in emodin-induced hepatotoxicity, the results showed that emodin can decrease MMP and trigger ER stress with Ca2+ overloading and the expression of ATF4 increasing, further resulted with increased apoptosis in L02 cells and mice mortality rate, while the changes were alleviated by CH223191. Furthermore, the 5-hydroxyemodin, a metabolite by emodin through CYP1A2 enzyme, showed more severe hepatotoxicity compared to emodin. CONCLUSIONS: Our results validated that the metabolism of emodin to 5-hydroxyemodin by CYP1A played an important role in the hepatocellular toxicity of emodin and provided evidence that CYP1A1 and AhR could be used to predict and validate patient-specific liver injury of PMT or other herbs containing emodin.

Mechanisms and Molecular Targets of Compound Danshen Dropping Pill for Heart Disease Caused by High Altitude Based on Network Pharmacology and Molecular Docking.

Compound Danshen dropping pill (CDDP), a famous Chinese medicine formula, has been widely used to treat high-altitude heart disease in China. However, its molecular mechanisms, potential targets, and bioactive ingredients remain elusive. In this study, network pharmacology, molecular docking, and validation experiments were combined to investigate the effective active ingredients and molecular mechanisms of CDDP in the treatment of high-altitude heart disease. Tan IIA may be the main active component of CDDP in the treatment of high-altitude heart disease via HIF-1/PI3K/Akt pathways.

Biochemical and molecular impacts of glyphosate-based herbicide on the gills of common carp.

Glyphosate (GLY)-based herbicide, one of the most widely used herbicides, might cause a series of environmental problems and pose a toxicological risk to aquatic organisms. However, data on the potential hazard and toxicity mechanism of GLY to fish gills are relatively scarce. In this study, a subacute toxicity test of common carp (Cyprinus carpio L.) treated with commercial GLY at 52.08 and 104.15 mg L-1 for 7 d was conducted. The results revealed that GLY exposure significantly inhibited Na+/K+-ATPase and increased AST and ALT activities in the fish gills. The biochemical assays results revealed that GLY treatment remarkably altered the transcriptional levels of HSP70 and HSP90; inhibited the activities of SOD, CAT, GPx, GR, and T-AOC; reduced the contents of GSH, but remarkably promoted MDA and PC contents, suggesting that GLY exposure induced oxidative stress and lipids and proteins damage in the carp gills. Further research revealed that GLY exposure also promoted expression of NF-κB, iNOS, IL-1ß, IL-6, IL-8, and TNF-α; altered the levels of IL-10 and TGF-ß, indicating that GLY exposure induced inflammatory response in the fish gills. Additionally, we found that GLY exposure activated apaf-1 and bax and inhibited bcl-2, induced caspase-9 and caspase-3 expression and caused remarkable histological damage in the fish gills. These results may further enriches the toxicity mechanistic theory of GLY to fish gills, which may be useful for the risk assessment of GLY and aquatic organism protection.

Electric field gradient quadrupole Raman modes observed in plasmon-driven catalytic reactions revealed by HV-TERS.

Electric field gradient quadrupole Raman modes are observed in plasmon-driven chemical reactions investigated with high vacuum tip-enhanced Raman spectroscopy (HV-TERS). TER spectra reveal that 4-nitrobenzenethiol (4NBT) catalytically dimerizes to dimercaptoazobenzene (DMAB) under an HV-TERS setup. More importantly, we find that the electric field gradient leads to strong enhancement of the infrared (IR)-active modes of DMAB. The observation of both the Raman-active and IR-active modes of DMAB provides spectral evidence for ultrasensitive chemical analysis.

Insights into the nature of plasmon-driven catalytic reactions revealed by HV-TERS.

The nature of plasmon-driven chemical reactions is experimentally investigated using high vacuum tip-enhanced Raman spectroscopy (HV-TERS). It is revealed that the coupling between the tip and the substrate can produce intense plasmon resonance, which then decays to produce sufficient hot electrons and thus catalyses the chemical reaction. The photoelectron emission from the laser illuminated silver substrate alone cannot drive the reaction.