Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 27
Filtrar
1.
BMC Nephrol ; 23(1): 198, 2022 05 28.
Artículo en Inglés | MEDLINE | ID: mdl-35643467

RESUMEN

BACKGROUND: Pyrethroids are used for insect control. They act on voltage-gated sodium channels in neurons. Lambdacyhalothrin (LCH) is a type II pyrethroid producing choreoatetosis and salivation in rodents. Vascular endothelial growth factor (VEGF) expression in the kidney is high in the glomerular podocytes and kidney tubules. VEGF receptor 2 (VEFGR2) is the main mediator in angiogenesis and it regulates blood vessel permeability. Lack of VEGF in podocytes impairs filtration. The nuclear factor κB (NFκB) is widely known as an important mediator of inflammation. The aim of the study was to check if subacute oral intoxication with 0.1LD50 of LCH affects kidney size, function and VEGFR2 and NFκB in mice kidneys. METHODS: A total of 32 Albino Swiss mice was used: females controls, males controls, females receiving 2 mg/kg LCH, males receiving 2 mg/kg LCH orally for 7 days. On day 8 animals were sacrificed, blood and kidneys were obtained. Kidney mass was determined, creatinine concentration was measured in blood sera, VEGFR2 and NFκB in kidney homogenate supernatant with ELISA kit. RESULTS: There was no statistically significant differences in kidney mass, creatinine concentration in blood sera nor NFκB but mean VEGFR2 concentration in the kidneys of females exposed to LCH was 128.01 ng/ml and showed statistically significant difference in comparison with control females. There was no statistically significant difference between VEGFR2 concentration in the kidneys of males exposed to LCH and control males. CONCLUSION: The VEGFR2 increases in the course of LCH intoxication in females probably due to the protective effect of oestrogens.


Asunto(s)
Piretrinas , Factor A de Crecimiento Endotelial Vascular , Animales , Creatinina/metabolismo , Femenino , Humanos , Riñón/metabolismo , Masculino , Ratones , FN-kappa B/metabolismo , Nitrilos , Piretrinas/metabolismo , Piretrinas/toxicidad , Receptores de Factores de Crecimiento Endotelial Vascular , Factor A de Crecimiento Endotelial Vascular/metabolismo , Factores de Crecimiento Endotelial Vascular/metabolismo
2.
Pol Merkur Lekarski ; 38(227): 258-62, 2015 May.
Artículo en Polaco | MEDLINE | ID: mdl-26039019

RESUMEN

UNLABELLED: Patients with cardiovascular diseases, including those with the symptoms of metabolic syndrome (MS), are recommended regular exercise but many studies indicate its role in the production of reactive oxygen species. Vitamin C supplementation may enhance the antioxidant barrier in MS patients. AIM: The aim of the study was to assess the impact of regular physical activity (PA)and vitamin C supplementation on plasma vitamin A, C and E levels in patients with MS. MATERIALS AND METHODS: The study included 62 patients with MS according to International Diabetes Federation criteria, 32 men and 30 women, aged 38-57 years (mean age 51,24 ± 5,29 years). The patients were divided in two groups: group I (MS+PA) - 31 patients with recommended regular physical activity; group II ( MS+PA+C) - 31 patients with recommended regular physical activity and vitamin C supplementation per os. The control group consisted of 23 healthy individuals without MS, 17 men and 6 women, aged 49-56 years (mean age 53,21 ± 3,6 years), who were not recommended any vitamin supplementation nor physical activity. Plasma vitamin A, C and E levels were estimated in MS patients with spectrophotometry using T60V spectrophotometer (PG Instruments) before and after regular exercise with and without vitamin C supplementation. In the control group plasma levels of antioxidant vitamins were assessed only once. RESULTS: The plasma vitamin A, C and E levels were significantly lower (p<0,05) in MS patients than in the control group. After 6 weeks of regular physical activity a significant fall in plasma levels of antioxidant vitamins was observed in MS patients. In the group of patients with regular physical activity and vitamin C supplementation there was detected a significant rise in the level of all the tested vitamins close to the levels in control group. CONCLUSIONS: Regular physical activity enhances the decrease in plasma antioxidant vitamin level in patients with MS. Vitamin C supplementation conducted in parallel with regular physical activity normalize plasma vitamin A, C and E levels in these patients.


Asunto(s)
Ácido Ascórbico/sangre , Ácido Ascórbico/uso terapéutico , Ejercicio Físico/fisiología , Síndrome Metabólico/fisiopatología , Síndrome Metabólico/terapia , Vitamina A/sangre , Vitamina E/sangre , Adulto , Antioxidantes/fisiología , Suplementos Dietéticos , Femenino , Humanos , Masculino , Persona de Mediana Edad
3.
Pol Merkur Lekarski ; 36(215): 320-3, 2014 May.
Artículo en Polaco | MEDLINE | ID: mdl-24964509

RESUMEN

UNLABELLED: Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting development of cardiovascular diseases. In the pathogenesis of MS there participate reactive oxygen species which are excessively produced in such elements of MS as hyperglycemia, insulin resistance and obesity. Vitamins A, C and E are an important part of the non-enzymatic antioxidative barrier in humans. The aim of the study was to estimate plasma vitamin A, C and E levels in patients with symptoms of MS. MATERIAL AND METHODS: The study included 68 patients with symptoms of MS according to International Diabetes Federation criteria (2005), 37 men and 31 women, aged 34-65 years (mean age 57, 76 +/- 8, 29 years). The control group consisted of 24 healthy individuals without MS, 18 men and 6 women, aged 49-67 (mean age 58, 5 +/- 5, 6 years). Plasma vitamin A, C and E levels were estimated in patients and the control group with spectrophotometry using T60V spectrophotometer (PG Instruments). RESULTS: The plasma vitamin A, C and E levels were significantly lower (p < 0.05) in MS patients than in the healthy individuals without symptoms of MS. The most significant differences in the level of antioxidative vitamins in both groups were related to vitamin C and vitamin E. CONCLUSIONS: The decreased level of vitamins A, C and E points to the weakening of antioxidative barrier in patients with MS.


Asunto(s)
Ácido Ascórbico/sangre , Síndrome Metabólico/sangre , Vitamina A/sangre , Vitamina E/sangre , Adulto , Anciano , Antioxidantes/metabolismo , HDL-Colesterol/sangre , Femenino , Humanos , Masculino , Persona de Mediana Edad
4.
Pol Merkur Lekarski ; 35(205): 18-21, 2013 Jul.
Artículo en Polaco | MEDLINE | ID: mdl-23984600

RESUMEN

UNLABELLED: Lung cancer is one of the most common cancer types and it usually takes the form of non-small cell lung cancer (NSCLC). ROS take part in the process of carcinogenesis. What more, chemotherapy used in cancer treatment augments their production, leading to the weakening of the antioxidative barrier. As a result in cancer patients undergoing chemotherapy the reduction-oxidation processes are imbalanced. Vitamins A, C and E form an important part of the nonenzymatic antioxidative barrier in humans. THE AIM OF THE STUDY was an assessment of concentrations of A, C and E vitamins in the plasma of patients with NSCLC before and after chemotherapy MATERIAL AND METHODS: 25 first-line chemotherapy patients with inoperable NSCLC have undergone examination, including 20 men and 5 women aged between 50-75 years (average age 62.6 +/- 6.1 years). 24 healthy individuals including 18 men and 6 women aged between 49-71 years (average age 59.5 +/- 6.6 years) formed a control group. In cancer patients the concentration of vitamins A, C and E was assessed by spectrophotometry using T60V spectrophotometer (PG Instruments) before and after first-line chemotherapy, while in control group it was assessed only once. RESULTS: The concentration of A, C and E vitamins in plasma of NSCLC patients was lower (p < 0.05) than in the control group. After 6 weeks of chemotherapy another significant drop in vitamin concentrations in NSCLC patients was observed (p < 0.05) and was biggest for vitamin C (39.1%). CONCLUSIONS: Lowering of A, C and E vitamins concentrations in the plasma of NSCLCpatients suggests a weakening of antioxidative barrier. Chemotherapy leads to further fall in the concentration of those vitamins in patients' plasma.


Asunto(s)
Ácido Ascórbico/sangre , Carcinoma de Pulmón de Células no Pequeñas/sangre , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Neoplasias Pulmonares/sangre , Neoplasias Pulmonares/tratamiento farmacológico , Vitamina A/sangre , Vitamina E/sangre , Antineoplásicos/uso terapéutico , Femenino , Humanos , Masculino , Persona de Mediana Edad
5.
Pol Merkur Lekarski ; 35(207): 136-40, 2013 Sep.
Artículo en Polaco | MEDLINE | ID: mdl-24224449

RESUMEN

UNLABELLED: One of the main after-effects of chemotherapy used in cancer treatment is an augmented production of reactive oxygen species (ROS). In turn ROS become a source of unwanted side effects of chemotherapy, often forcing the discontinuation of the therapy. Ascorbic acid (vitamin C), being an antioxidant, can strengthen the antioxidative barrier of an organism. The aim of the study was an assessment of the concentrations of A, C and E vitamins in the plasma of NSCLC patients undergoing chemotherapy supplemented with vitamin C. MATERIAL AND METHODS: 25 first-line chemotherapy patients with inoperable NSCLC, including 19 men and 6 women aged between 37-73 years (average age 60.1 +/- 8.8 years) have undergone the examination. Their chemotherapy has been supplemented with ascorbic acid (vitamin C dose of 600 mg per 24 hours). Control group consisted of 24 healthy individuals, including 18 men and 6 women aged between 49-71 years (average age 59.5 +/- 6.6 years). In cancer patients the concentration of A, C and E vitamins was assessed by spectrophotometry using T60V spectrophotometer (PG Instruments) before and after first-line chemotherapy which was supplemented with vitamin C. In control group the concentrations of antioxidative vitamins was assessed only once. RESULTS: In comparison to the control group the concentrations of the A, C and E vitamins in the plasma of NSCLC patients was significantly lower (p < 0.05). After 6 weeks of chemotherapy supplemented with vitamin C a significant rise of concentrations (p < 0.05) of all the vitamins tested for was observed. The biggest rise was noted for vitamin C (99.8%). CONCLUSIONS: The supplementation of the chemotherapy of NSCLC patients with C vitamin leads to rise of the low concentrations of A, C and E vitamins in the plasma. This suggests strengthening of the antioxidative barrier in patients.


Asunto(s)
Antioxidantes/análisis , Ácido Ascórbico/administración & dosificación , Carcinoma de Pulmón de Células no Pequeñas/sangre , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Neoplasias Pulmonares/sangre , Neoplasias Pulmonares/tratamiento farmacológico , Vitaminas/sangre , Adulto , Anciano , Antineoplásicos/uso terapéutico , Suplementos Dietéticos , Femenino , Interacciones Alimento-Droga , Humanos , Masculino , Persona de Mediana Edad , Vitamina A/administración & dosificación , Vitamina A/sangre , Vitamina E/administración & dosificación , Vitamina E/sangre
6.
Pol Merkur Lekarski ; 29(174): 377-81, 2010 Dec.
Artículo en Polaco | MEDLINE | ID: mdl-21298989

RESUMEN

It has been found during last years that vitamins A, C, E and carotenoids, besides previously recognized functions of preventing particular lipido- and avitaminosis, significantly participate in the protection of the human body against oxidation stress that is characterized by balance disturbance between speed of free radical creation and reactive oxygen forms with pace of their neutralization by enzymes and antioxidants. The antioxidative system creates special protective barrier which includes numerous chemical form with antiperoxidative proprieties and vitamins are the most important part of it. The aim of this work was the profile of occurrence and antioxidative action of the vitamins A, C and E.


Asunto(s)
Antioxidantes/farmacología , Ácido Ascórbico/farmacología , Vitamina A/farmacología , Vitamina E/farmacología , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Humanos , Vitamina A/metabolismo , Vitamina E/metabolismo
7.
Pol Merkur Lekarski ; 26(151): 52-6, 2009 Jan.
Artículo en Polaco | MEDLINE | ID: mdl-19391508

RESUMEN

UNLABELLED: A nitrogen ring is present in many biological molecules, which play an important part in cell division as well as in genetic information transfer. It is also a part of the purine system, which builds DNA and has potential places/spots of platinum drug co-ordination. Therefore, it may be hoped, that platinum(II) complexes with diazol ligands will have considerable affinity for DNA binding sites. The work was aimed at assessment of biological activity and especially of cytotoxic activity, of new complex connections of the selected diazols with Pt(II) and Pd(II) ions. MATERIALS AND METHODS: The MTT test is aimed at the assessment of cytotoxicity of chemical compounds. It consists in the colorimetric determination of a product--farmazon, formed after adding MTT (bromide 3[4,5-dimetylo-2-ilo]-2,5-difenylotetrazolu) to the culture suspension in the presence of the compound subject to the test. All the tested Pt(II) complexes before adding to the cell cultures were dilluted in DMF. MTT tests for individual complexes were conducted on two cell lines L1210 and P388. RESULTS: Compounds 13, 21 and 25 on P388 neoplastic cell revealed about three times smaller in-vitro cytotoxicity, whereas platinum(II) and palladium(II) complexes 13, 21, 25, 43, 46, revealed cytotoxicity being about 3-4 times smaller than the reference cisplatin. Complex 45 revealed eight times smaller activity and complex 44 revealed significantly small, about two hundred smaller activity. CONCLUSION: Based on the MTT tests it may be concluded that compounds 13, 21 and 25 reveal selective cytotoxicity to P388 neoplastic cells.


Asunto(s)
Antineoplásicos/farmacología , Leucemia L1210/tratamiento farmacológico , Leucemia P388/tratamiento farmacológico , Compuestos Organometálicos/farmacología , Animales , Antineoplásicos/química , Línea Celular Tumoral , Cisplatino/farmacología , Ratones , Compuestos Organometálicos/química , Paladio , Células Tumorales Cultivadas
8.
Pol Merkur Lekarski ; 26(151): 57-61, 2009 Jan.
Artículo en Polaco | MEDLINE | ID: mdl-19391509

RESUMEN

UNLABELLED: A pyrazole system is incorporated in many biological molecules which play an important part in genetic processes. It seems that co-ordinating biological molecules, which would serve as carriers, should contribute to minimising the toxic effects of the potential platinic drugs. AIM OF THE STUDY: Assessment of in vivo biological activity, and in particular of antineoplastic activity of new platinum(II) and palladium(II) complexes. MATERIAL AND METHODS: The assessment of toxicity of the compounds was made with the Deichmann and Le Blanck method. The antineoplastic activity of the new complex compounds--pyrazole derivatives with Pt(II) and Pd(II) ions was tested on mouse L1210 leukemia cell culture and on lymphatic leukemia P388. BDF1 or CDF1 mice divided into groups of 5-9 animals were subject to the tests. RESULTS: Complexes 13, 21, 25, 16, 24 and 28 did not reveal any antineoplastic activity to the mouse L1210 leukemia, whereas complexes 13, 21 and 25 revealed in-vivo antineoplastic activity to the P388 leukemia, extending the mouse's survival time by about 50%. The control group consisted of mice which were administered a 14% methylcellulose solution. CONCLUSIONS: As a result of the tests conducted to asses the in-vivo antineoplastic activity it was found that the 25 complex demonstrates the strongest activity to the P388 leukemia. It may be presumed that it is caused by trans configuration of the complex predisposing to the creation of interstrand cross-links. It may be also caused by the presence of the chloromethyl substituents, located on N1 nitrogen atom of the ligand, which increases the capacity of nitrogen N7 guanine alkylation in DNA.


Asunto(s)
Antineoplásicos/farmacología , Leucemia L1210/tratamiento farmacológico , Leucemia P388/tratamiento farmacológico , Compuestos Organoplatinos/farmacología , Animales , Antineoplásicos/química , Línea Celular , Cisplatino/farmacología , Ratones , Compuestos Organoplatinos/química , Células Tumorales Cultivadas
9.
Pol Merkur Lekarski ; 24(140): 151-7, 2008 Feb.
Artículo en Polaco | MEDLINE | ID: mdl-18634274

RESUMEN

Contemporary coordinative chemistry is still searching new substances that might constitute good ligands for transient metal ions: Cu(II), Ni(II), Co(II), Pt(II), Pd(II) oraz Cr(III). Pyrazole and its derivatives are able to make complexes with transient metal ions. There is a connection between pharmacological and chemical structure among pyrazole derivatives used in a medical treatment. A chemical division is coincident with a pharmacological division. Pyrazole-5 derivatives are mainly analgesic, antipyretic and less anti-inflammatory and diastolic but pyrazolidyn-3.5-dion derivatives are anti-inflammatory. The main part of non-steroid anti-inflammatory drugs belonging to pyrazolone derivates has a phenyl group at the first position. The activity and toxicity of this drugs are dependent on groups being at the fourth position. For example, the dimethylamino group makes aminophenazon antianalgetic, and yet is also responsible for a big toxicity. Azole derivatives are antimycotic and are ingredients of some ointments and creams. Chemical groups at the fourth position may determine the activity and the toxicity. These days a great attention is paid on a synthesis which is linking pyrazole and ions of platinum Pt(II) and palladium Pd(III) due to anticancer properties but pharmacology is not known very well.


Asunto(s)
Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/farmacología , Antineoplásicos/química , Pirazoles/química , Pirazoles/farmacología , Antineoplásicos/farmacología , Humanos
10.
Adv Clin Exp Med ; 27(1): 77-82, 2018 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-29521046

RESUMEN

BACKGROUND: Thus far, the pathogenesis of these intestinal tumors has not been fully explained. However, the analysis of risk factors and research regarding their formation that have continued for 3 decades have allowed us to demonstrate a significant role of oxidative stress in the processes leading to the development of cancer in the large intestine as well as in some other organs. OBJECTIVES: The aim of the study was to examine the level of anti-oxidative status and the degree of oxidative protein damage in patients with varying severity of colonic adenocarcinoma (CAC) in relation to healthy individuals. MATERIAL AND METHODS: The study involved 4 groups (A-D) of patients with increasing severity of CAC stages according to Dukes' classification and a control group of healthy volunteers. Total antioxidant capacity (TAC) of blood plasma, as well as carbonyl (C=O) group contents in blood plasma proteins as a product of their oxidative damage, were estimated in all participants. Both parameters were determined by spectrophotometric methods using commercial kit to test TAC and 2,4-dinitrophenylhydrazine to assay the contents of C=O groups. RESULTS: In each of the studied groups, A-D, a statistically significant reduction in the TAC values was noted relative to the control group, which progressed with increased severity of CAC stages: 1.783 mmol/L vs 1.191 mmol/L (group A), 1.07 mmol/L (group B), 0.931 mmol/L (group C), and 0.899 mmol/L (group D). At the same time, significantly increased contents of protein C=O groups were observed compared to the controls, also progressive in the course of growing CAC severity: 0.496 nmol/mg protein vs 0.57 nmol/mg protein (group A), 0.689 nmol/mg protein (group B), 0.804 nmol/mg protein (group C), and 1.054 nmol/mg protein (group D). CONCLUSIONS: The CAC-related oxidative stress considerably reduces the systemic anti-oxidative status and increases the protein damage; both those changes become worse in parallel with the progression of this cancer.


Asunto(s)
Adenocarcinoma/metabolismo , Antioxidantes/metabolismo , Neoplasias del Colon/metabolismo , Estrés Oxidativo/fisiología , Proteínas Sanguíneas , Humanos
11.
Pol Merkur Lekarski ; 22(130): 314-7, 2007 Apr.
Artículo en Polaco | MEDLINE | ID: mdl-17684934

RESUMEN

The big problem of high-developed community is hypokinesis - clinical state generated by long-lasting immobilization. Virtually all body systems are affected by immobilization. Hypokinesis intensify production of reactive oxygen species (ROS). The detoxification of ROS is especially important for muscles because myocytes are particularly weekly protecting against oxidative injury. Hypokinesis is a case of increase lipid peroxydation process in muscle tissue and increase aggregation of thrombocytes and high decreased of antioxidative enzymes (superoxide dismutase, glutatione peroxide, catalase) and concentration of vitamin E in blood. As a result muscle lost mass with receptivity on thrombosis. Rehabilitation is very important because it activated antioxidant defense system to detoxification ROS. Supplementation by antioxidative vitamins and antithrombosis treatment are very helpful.


Asunto(s)
Hipocinesia/metabolismo , Inmovilización/efectos adversos , Enfermedades Musculares/metabolismo , Especies Reactivas de Oxígeno/efectos adversos , Antioxidantes/metabolismo , Humanos , Hipocinesia/complicaciones , Peroxidación de Lípido/fisiología , Enfermedades Musculares/etiología , Especies Reactivas de Oxígeno/metabolismo , Factores de Tiempo
12.
Pol Merkur Lekarski ; 22(131): 404-5, 2007 May.
Artículo en Polaco | MEDLINE | ID: mdl-17679381

RESUMEN

Ascorbic acid is known antioxidant protecting many organs and tissues. In some circumstances can act as prooxidant, particullary when coexists with high concentration of ferrum and copper jons. Condition like that can take place for example in liver. In this research Vitamin C was administered to the mice in increasing dosages, then the concentration of TBARS-prooxidant markers was measured in homogenats of their livers. The outcomes of the research are equivocal.


Asunto(s)
Antioxidantes/administración & dosificación , Ácido Ascórbico/administración & dosificación , Hígado/metabolismo , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Animales , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Suplementos Dietéticos , Relación Dosis-Respuesta a Droga , Femenino , Peroxidación de Lípido/efectos de los fármacos , Hígado/química , Ratones , Ratones Endogámicos BALB C , Oxidación-Reducción/efectos de los fármacos , Sustancias Reactivas al Ácido Tiobarbitúrico/química , Distribución Tisular
13.
Pol Merkur Lekarski ; 22(131): 391-4, 2007 May.
Artículo en Polaco | MEDLINE | ID: mdl-17679377

RESUMEN

Gallbladder and biliary tract cancer is a serious clinical problem. In-spite of wide range of new diagnostic and therapeutic methods, the significant improvement of treatment results, has not been noticed so far. The research about prevention methods, seems to be important, among the ways of improvement of the diagnosis and therapy outcomes of these diseases. It is related for example to study about one of the cause of biliary tract carcinogenesis--the imbalance between production of reactive oxygen species (ROS) and their inactivation by the antioxidative barrier. One of the components of this organism protection complex are antioxidative vitamins. The aim of this study was to measure the concentration of antioxidative vitamins (A, C, E, and beta-carotene) in serum, in patients with cancer of gallbladder and and biliary tract, in comparison to the results of healthy volunteers. The study groups comprised of 56 patients, both sexes with acute and chronic cholecystitis and with above-mentioned neoplasmas. The results shoved, that concentration of antioxidative vitamins in serum of patients with gallbladder and biliary tract cancer, was significantly decreased. The outcomes of this research, seem to confirm the participation of antioxidative barrier in inhibition of carcinogenesis of in those important parts of digestive system.


Asunto(s)
Antioxidantes/metabolismo , Neoplasias de los Conductos Biliares/sangre , Neoplasias de la Vesícula Biliar/sangre , Vitaminas/sangre , Adulto , Anciano , Anciano de 80 o más Años , Ácido Ascórbico/sangre , Carotenoides/sangre , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Vitamina A/sangre , Vitamina E/sangre
14.
Pol Merkur Lekarski ; 23(136): 255-8, 2007 Oct.
Artículo en Polaco | MEDLINE | ID: mdl-18293846

RESUMEN

UNLABELLED: In recent years we observed increased interest of free radical processes accompanying varying physiological and pathological reactions. THE AIM OF THIS STUDY was an evaluation some of serum microelements (zinc, manganium, chromium, selenium) in serum and antioxidative defense in youth with hypertension. MATERIAL AND METHODS: The group of 88 youth aged 9-18 with normal blood pressure, prehypertension and hypertension was examined. Blood was taken to examine microelements content and antioxidative barrier activity. Microelements content was estimated with the method ICP-AES. Superoxide dismuthase activity in red blood cells was estimate with the method by Misra and Fridovich, glutatione peroxidase activity with the method by Sedlak and Lindsay in modification by Little and O'Brien and catalase activity with the method of Beers and Sizer. RESULTS: In subgroup with higher blood pressure we observed decreased tendency in enzymatic antioxidant activity (Cat, GSA-Px, SOD) in red blood cells and lowering levels of vitamins C and E in comparison to persons with normal blood pressure. Concentrations of estimated microelements drowed decreased levels in persons with hypertension. CONCLUSIONS: Results of our study suggest that disturbance in pro - and anti-oxidative balance observed in youth with first state of hypertension may play an important role in pathogenesis of hypertension.


Asunto(s)
Antioxidantes/análisis , Hipertensión/sangre , Adolescente , Niño , Cromo/sangre , Femenino , Humanos , Masculino , Manganeso/sangre , Selenio/sangre , Zinc/sangre
15.
Int J Occup Med Environ Health ; 30(5): 695-703, 2017 Jul 14.
Artículo en Inglés | MEDLINE | ID: mdl-28584329

RESUMEN

OBJECTIVES: The increasing number of devices emitting electromagnetic radiation (EMR) in people's everyday life attracted the attention of researchers because of possible adverse effects of this factor on living organisms. One of the EMR effect may be peroxidation of lipid membranes formed as a result of free radical process. The article presents the results of in vitro studies aimed at identifying changes in malondialdehyde (MDA) concentration - a marker of lipid peroxidation and antioxidant role of vitamin A during the exposure of blood platelets to electromagnetic radiation generated by liquid-crystal-display (LCD) monitors. MATERIAL AND METHODS: Electromagnetic radiation emitted by LCD monitors is characterized by parameters: 1 kHz frequency and 220 V/m intensity (15 cm from display screen). The time of exposure was 30 and 60 min. The study was conducted on porcine blood platelets. The samples were divided into 6 groups: unexposed to radiation, unexposed + vitamin A, exposed for 30 min, exposed for 30 min + vitamin A, exposed for 60 min, exposed for 60 min + vitamin A. RESULTS: The MDA concentration in blood platelets increases significantly as compared to control values after 60 min of exposure to EMR. A significant decrease in MDA concentration after the addition of vitamin A was noticed. In the blood samples exposed to EMR for 30 and 60 min the MDA concentration was significantly increased by addition of vitamin A. CONCLUSIONS: The results show the possibly negative effect of electromagnetic radiation on the cellular membrane structure manifested by changes in malondialdehyde concentration and indicate a possible protective role of vitamin A in this process. Int J Occup Med Environ Health 2017;30(5):695-703.


Asunto(s)
Antioxidantes/administración & dosificación , Plaquetas/efectos de la radiación , Membrana Celular/efectos de la radiación , Radiación Electromagnética , Vitamina A/administración & dosificación , Animales , Plaquetas/metabolismo , Terminales de Computador , Peroxidación de Lípido/efectos de la radiación , Malondialdehído/metabolismo , Porcinos
16.
Adv Clin Exp Med ; 26(4): 581-586, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-28691410

RESUMEN

BACKGROUND: The increasing incidence of metabolic diseases such as obesity or diabetes have made them a major public health problem. Increasing oxidative stress induced by reactive oxygen species, which initiate the oxidative adverse changes in the cell, is mentioned, among other risk factors, to underlie these diseases. Vitamin A, C and E are listed among the non-enzymatic mechanisms counteracting this phenomenon. Vitamin D deficiency is also associated with cardiovascular diseases. OBJECTIVES: The aim of the study was to assess the risk of vitamin A, C, E and D deficit in the plasma of metabolic syndrome (MS) patients. MATERIAL AND METHODS: The study included 191 patients with MS and 98 subjects without MS. Loglinear analysis was used in the assessment of mutual interactions between the vitamin concentration and the analysis of classification by ROC curves to predict the frequency of vitamin deficiency in MS patients. RESULTS: A correlation was found between the plasma level of vitamins in the group of MS patients. Vitamin A concentration correlated with that of vitamin C (r = 0.51, p = 0.0000), vitamin D (r = 0.49, p = 0.0000) and E (r = 0.32, p = 0.0001). The plasma level of vitamin D correlated with the level of vitamin E (r = 0.46, p = 0.00000) and vitamin C (r = 0.37, p = 0.0000). Regression analysis showed a correlation between the concentration of the tested vitamins in patients with MS. Interactions were observed between vitamins C and A and between C and D. HDL cholesterol level was lower in patients with vitamin A deficiency compared to patients with its normal level. CONCLUSIONS: The plasma levels of vitamin A, C, E and D were significantly lower in patients with MS than in healthy subjects and they mutually correlated with each other. The normalization of glucose and HDL level may contribute to the regulation of the concentration of vitamin A in patients with MS.


Asunto(s)
Deficiencia de Ácido Ascórbico/etiología , Síndrome Metabólico/complicaciones , Deficiencia de Vitamina A/etiología , Deficiencia de Vitamina D/etiología , Deficiencia de Vitamina E/etiología , Adulto , Anciano , Ácido Ascórbico/sangre , Estudios de Casos y Controles , HDL-Colesterol/sangre , Femenino , Humanos , Masculino , Síndrome Metabólico/sangre , Persona de Mediana Edad , Especies Reactivas de Oxígeno/metabolismo , Riesgo , Vitamina A/sangre , Vitamina D/sangre , Vitamina E/sangre
17.
Pol Merkur Lekarski ; 20(117): 261-4, 2006 Mar.
Artículo en Polaco | MEDLINE | ID: mdl-16780250

RESUMEN

After an ischemic insult, a multi-faceted complex cascade of biochemical reactions occurs that ultimately causes neurons death. Above reactions exert an influence on: immunological changes (activation of the complement system and the generation of antibodies), increased inflammation (actions of proinflammatory cytokines and chemokines), the production of reactive oxygen species leading to oxidative stress, diminished mitochondrial function and activation of apoptotic pathways. There is also intensive release and wrong reversible escapement many of neurotransmitters. The last one throught oxidative desamination are one of the main sources most of free radicals. Central nervous system is particularly susceptible to ROS-induced damage due to the high oxygen demands of the brain and low concentration of endogenous antioxidants. lts refer both enzymatic antioxidants: catalase, glutathione peroxidase, glutathione reductase, superoxide dismutase and nonenzymatic antioxidants glutathione, vitamin a, c, e, coenzym Q, uric acid etc. Presently there are no neuroprotective treatments and prevention. One way of treatment testing in clinical trials is hypothermia inhibits above-mentioned processes.


Asunto(s)
Antioxidantes/metabolismo , Isquemia Encefálica/fisiopatología , Animales , Isquemia Encefálica/terapia , Proteínas del Sistema Complemento/inmunología , Humanos , Mediadores de Inflamación/metabolismo , Estrés Oxidativo/inmunología
18.
Pol Merkur Lekarski ; 20(119): 609-14, 2006 May.
Artículo en Polaco | MEDLINE | ID: mdl-16875174

RESUMEN

The article presents current state of knowledge about antioxidative action of vitamin E, enabling significant broadening of its possible application in medicine. Mechanisms are discussed due to which it takes a very important part in protection of an organism against effects of oxidative stress. Followed from here consequences of this vitamin deficiencies were indicated. Detailed review was made of its role in counteracting diseases of the largest social significance: cancer and cardiovascular system diseases. Attention was paid to observed controversies and discrepancies in therapeutic effects. Unfavourable effects that could appear with application of large doses of vitamin E, typical for antioxidative therapy, were characterised.


Asunto(s)
Antioxidantes/farmacología , Antioxidantes/uso terapéutico , Vitamina E/farmacología , Vitamina E/uso terapéutico , Enfermedades Cardiovasculares/prevención & control , Humanos , Neoplasias/prevención & control , Estrés Oxidativo
19.
Endokrynol Pol ; 57(5): 494-500, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17133314

RESUMEN

INTRODUCTION: Osteoporosis is a systemic disease of the skeletal system characterised by reduced bone strength leading to increased risk of fracture. Bone strength is a combined derivative of bone mineral density (BMD) and of bone tissue quality. Osteoporosis is a serious medical problem because of its complications, most frequently manifesting itself in spine fractures, fractures of distal sections of the forearm and, in later periods of life, hip fractures. Reduced BMD is an independent risk factor of osteoporosis. The goal of the study was an evaluation of bone mineral density of the population inhabiting the micro-region of Brzesc Kujawski from the 11(th) century until the beginning of the 19th century. MATERIAL AND METHODS: Human bones obtained from archaeological excavations at four archaeological sites: Kolonia (11th-13th centuries), SBK-4 (12th-16th centuries), Fara (14th-17th centuries) and Swiety Duch (16th-19th centuries) and from the collections of the Katedra of Anthropology of the University of Lódz were subjected to study. Bone mineral densities of the skeleton populations were compared with those of the control group, namely the present living population of the Lódz Province. The incidence of osteoporosis was evaluated by densitometric assessment, which was performed by dual energy X-ray absorptiometry (DXA) on a DPX device (LUNAR, USA). RESULTS: The densitometric measurements performed enabled differences to be identified in the mineral density of the osseous tissue. The skeletal groups studied were characterised by a significantly higher mean BMD than the contemporary living population. Changes in BMD indicative of osteopenia prevailed in women. CONCLUSIONS: On the basis of the results obtained it cannot definitively be stated that the skeletal groups studied, despite their significantly higher BMD, were affected by a lower risk of osteoporosis and its complications. A continuation of studies on the mineral content of bones and on the relationship between the mineral content and bone mineral density is required.


Asunto(s)
Densidad Ósea , Huesos/diagnóstico por imagen , Osteoporosis/epidemiología , Osteoporosis/historia , Absorciometría de Fotón , Adulto , Antropometría , Arqueología , Femenino , Antebrazo/diagnóstico por imagen , Historia del Siglo XV , Historia del Siglo XVI , Historia del Siglo XVII , Historia del Siglo XVIII , Historia del Siglo XIX , Historia del Siglo XX , Historia Medieval , Humanos , Vértebras Lumbares/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Osteoporosis/diagnóstico por imagen , Polonia/epidemiología , Prevalencia , Valores de Referencia , Distribución por Sexo , Columna Vertebral/diagnóstico por imagen
20.
Adv Clin Exp Med ; 25(4): 689-700, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27629843

RESUMEN

BACKGROUND: Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting the development of cardiovascular diseases. Reactive oxygen species, which are excessively produced in MS, participate in its pathogenesis. Vitamins A, C and E are an important part of the non-enzymatic antioxidative barrier in humans. OBJECTIVES: The aim of the study was to estimate plasma vitamin A, C and E levels and the intake of these vitamins from the diet in patients with MS. MATERIAL AND METHODS: The study included 182 patients with MS, 94 men and 88 women, aged 30-65 years (mean 57.31 ± 8.28 years). The control group was comprised of 91 subjects, 56 men and 35 women, aged 41-65 years (mean 57.75 ± 5.84 years). The MS diagnosis was based on IDF criteria. The determination of the serum level of vitamin A, C and E was performed using the spectrophotometric method. The food intake was assessed by 24-h dietary recall. RESULTS: The mean plasma vitamin A, C and E levels were significantly lower in MS patients than in the controls (p = 0.05). No correlation was found between vitamin A, C and E intake from the diet and their plasma concentrations in MS patients. Plasma vitamin A, C and E deficiency was observed significantly more often in MS patients than in the control group (15.38% vs. 2.19%, 79.12% vs. 8.79% and 60.45% vs. 5.49%, p < 0.0001, respectively). BMI was the one factor significantly affecting the mean value of vitamin A, C and E levels in MS patients. CONCLUSIONS: MS patients demonstrated significantly lower plasma levels of vitamin A, C and E compared to the healthy subjects. Lower plasma levels of antioxidant vitamins with their high intake from the diet indicate antioxidant barrier impairment in MS patients.


Asunto(s)
Antioxidantes/metabolismo , Ácido Ascórbico/sangre , Síndrome Metabólico/sangre , Vitamina A/sangre , Vitamina E/sangre , Adulto , Anciano , Deficiencia de Ácido Ascórbico/complicaciones , Deficiencia de Ácido Ascórbico/epidemiología , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Síndrome Metabólico/complicaciones , Persona de Mediana Edad , Deficiencia de Vitamina A/complicaciones , Deficiencia de Vitamina A/epidemiología , Deficiencia de Vitamina E/complicaciones , Deficiencia de Vitamina E/epidemiología
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA