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Indirect effects of agrochemicals on organisms via biotic interactions are less studied than direct chemical toxicity despite their potential relevance in agricultural landscapes. In particular, the role of species traits in characterizing indirect effects of pesticides has been largely overlooked. Moreover, it is still unclear whether such indirect effects on organisms are prevalent even when the organisms are exposed to direct toxicity. We conducted a mesocosm experiment to examine indirect effects of a herbicide (pentoxazone) on aquatic predatory insects of rice paddies. Because the herbicide selectively controls photosynthetic organisms, we assumed that the effects of the herbicide on predatory insects would be indirect. We hypothesized that phytophilous predators such as some Odonata larvae, which cling to aquatic macrophytes, would be more subject to negative indirect effects of the herbicide through a decrease in abundance of aquatic macrophytes than benthic, nektonic, and neustonic predators. Also, we crossed-applied an insecticide (fipronil) with herbicide application to examine whether the indirect effects of the herbicide on the assembling predators act additively with direct adverse effects of the insecticide. The herbicide application did not decrease the abundance of phytoplankton constitutively, and there were no clear negative impacts of the herbicide on zooplankton and prey insects (detritivores and herbivores). However, the abundance of aquatic macrophytes was significantly decreased by the herbicide application. Although indirect effects of the herbicide were not so strong on most predators, their magnitude and sign differed markedly among predator species. In particular, the abundance of phytophilous predators was more likely to decrease than that of benthic, nektonic, and neustonic predators when the herbicide was applied. However, these indirect effects of the herbicide could not be detected when the insecticide was also applied, seemingly due to fipronil's high lethal toxicity. Our study highlights the importance of species traits such as microhabitat use, which characterize biotic interactions, for predicting indirect effects of agrochemicals. Given that indirect effects of the chemicals vary in response to species traits and direct toxicity of other chemicals, efforts to explain this variation are needed to predict the realistic risks of indirect effects of agrochemicals in nature.
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Herbicidas , Insecticidas , Contaminantes Químicos del Agua , Animales , Insectos , Conducta Predatoria , ZooplanctonRESUMEN
A previous study claimed a differential behavioural resilience between spring or summer honey bees (Apis mellifera) and bumble bees (Bombus terrestris) after exposure to syrup contaminated with 125 µg L-1 imidacloprid for 8 days. The authors of that study based their assertion on the lack of body residues and toxic effects in honey bees, whereas bumble bees showed body residues of imidacloprid and impaired locomotion during the exposure. We have reproduced their experiment using winter honey bees subject to the same protocol. After exposure to syrup contaminated with 125 µg L-1 imidacloprid, honey bees experienced high mortality rates (up to 45%), had body residues of imidacloprid in the range 2.7-5.7 ng g-1 and exhibited abnormal behaviours (restless, apathetic, trembling and falling over) that were significantly different from the controls. There was incomplete clearance of the insecticide during the 10-day exposure period. Our results contrast with the findings reported in the previous study for spring or summer honey bees, but are consistent with the results reported for the other bee species.
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Abejas/fisiología , Insecticidas/toxicidad , Neonicotinoides/toxicidad , Nitrocompuestos/toxicidad , Pruebas de Toxicidad Subaguda , Animales , Abejas/efectos de los fármacos , Insecticidas/metabolismo , Neonicotinoides/metabolismo , Nitrocompuestos/metabolismoRESUMEN
Fipronil is one of the most effective insecticides to control the invasive ant Linepithema humile, but its effectiveness has been assessed without considering the genetic differences among L. humile supercolonies. We hypothesized that the susceptibility of the ant to fipronil might differ among supercolonies. If so, dosage and concentration of fipronil may need to be adjusted for effective eradication of each supercolony. The relative sensitivities of four L. humile supercolonies established in Hyogo (Japan) to fipronil baits were examined based on their acute toxicity (48-h LC(50)). Toxicities of fipronil to seven ground arthropods, including four native ant species, one native isopoda, and two cockroaches were also determined and compared to that of L. humile supercolonies using species sensitivity distributions. Marked differences in susceptibility of fipronil were apparent among the supercolonies (P < 0.008), with the 'Japanese main supercolony' (271 µg L(-1)) being five to ten times more sensitive to fipronil than other colonies (1183-2782 µg L(-1)). Toxicities to non-target species (330-2327 µg L(-1)) were in the same range as that of L. humile, and SSDs between the two species groups were not significantly different (t = -1.389, P = 0.180), suggesting that fipronil's insecticidal activity is practically the same for L. humile as for non-target arthropods. Therefore, if the invasive ant is to be controlled using fipronil, this would also affect the local arthropod biodiversity. Only the 'Japanese main supercolony' can be controlled with appropriate bait dosages of fipronil that would have little impact on the other species.
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Hormigas/efectos de los fármacos , Cucarachas/efectos de los fármacos , Insecticidas/farmacología , Isópodos/efectos de los fármacos , Pirazoles/farmacología , Animales , Hormigas/genética , Insecticidas/toxicidad , Especies Introducidas , Japón , Pirazoles/toxicidadRESUMEN
Systemic insecticides are one of the causes of Odonata declines in paddy fields. Since rising temperatures associated with global warming can contribute to strengthen pesticide toxicity, insecticide exposures under increasing temperatures may accelerate the decline of Odonata species in the future. However, the combined effects of multiple stressors on Odonata diversity and abundance within ecosystems under various environmental conditions and species interactions are little known. Here, we evaluate the combined effects of the insecticide fipronil and warming on the abundance of Odonata nymphs in experimental paddies. We show that the stand-alone effect of the insecticide exposure caused a significant decrease in abundance of the Odonata community, while nymphs decreased synergistically in the combined treatments with temperature rise in paddy water. However, impacts of each stressor alone were different among species. This study provides experimental evidence that warming could accelerate a reduction in abundance of the Odonata community exposed to insecticides (synergistic effect), although the strength of that effect might vary with the community composition in targeted habitats, due mainly to different susceptibilities among species to each stressor. Community-based monitoring in actual fields is deemed necessary for a realistic evaluation of the combined effects of multiple stressors on biodiversity.
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Insecticidas , Odonata , Animales , Insecticidas/análisis , Ecosistema , Neonicotinoides/toxicidadRESUMEN
Recent years have witnessed heightened scrutiny of the non-target sublethal effects of pesticides on behavioural and physiological traits of insects. Traditionally, attention has focused on investigating pesticides' primary modes of action, often overlooking the potential secondary mechanisms. This review brings forth the nuanced impacts of sublethal pesticide exposure on the immune system of target and non-target insect species. Pesticides, such as neonicotinoids, suppress immune response, while others, like certain organophosphates and some insect growth regulators (IGRs), appear to bolster immunocompetence. Beyond their individual impacts, the synergic effects of pesticide mixtures on insect immunity are garnering increasing interest. This review thus summarizes recent advances in the immunomodulatory effects of pesticides, detailing both mechanisms and consequences of such interactions. The implications of these effects for ecosystem preservation and viability of beneficial organisms, like pollinators and natural enemies of pests, are discussed. The review also considers further research directions on pesticide secondary modes of action and explores potential implications for integrated pest management (IPM) programs, as several model organisms studied are crop pest species. While current data provide an expansive overview of how insect innate immunity is modulated, concrete endpoints remain elusive requiring further research into pesticide secondary modes of actions.
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A passive sampler device suitable for monitoring of residues of the hydrophilic ionic herbicide amitrole in irrigation waterways was developed. Uptake of amitrole on styrenedivinylbenzene-reverse phase sulfonated Empore™ disks was linear and proportional to its water concentration over the range of 1-10 µg/L with a sampling rate of 23.1 mL/day under laboratory flow-through conditions. Performance of the sampler was evaluated by deployment in an agricultural irrigation drain for 10 days. The amount of amitrole adsorbed by the passive samplers compared well with the cumulative mean water concentrations calculated from daily spot samplings of the drain water.
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Riego Agrícola , Amitrol (Herbicida)/análisis , Residuos de Plaguicidas/análisis , Contaminantes Químicos del Agua/análisis , Calibración , Reproducibilidad de los ResultadosRESUMEN
Rapid climate warming has boosted biological invasions and the distribution or expansion polewards of many species: this can cause serious impacts on local ecosystems within the invaded areas. Subsequently, native species may be exposed to threats of both interspecific competition with invaders and temperature rises. However, effects of warming on interspecific interactions, especially competition between invader and native species remains unclear. To better understand the combined threats of biological invasions and warming, the effect of temperature on competitive interactions between two dragonfly species, the expanding Trithemis aurora from Southeast Asia and the Japanese native Orthetrum albistylum speciosum were assessed based on their foraging capacity. Although the stand-alone effect of temperature on foraging intake of the native dragonfly was not apparent, its intake significantly decreased with increasing temperatures when the invader T. aurora was present. Such reductions in foraging might lead to displacement of the native species through competition for food resources. This suggests that impacts of invader species against native species are expected to be more severe when interspecific competition is exacerbated by temperature rises.
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Assessment of ecological impacts of toxicants relies currently on extrapolation of effects observed at organismal or population levels. The uncertainty inherent to such extrapolations, together with the impossibility of predicting ecological effects of chemical mixtures, can only be resolved by adopting approaches that consider toxicological endpoints at a community or ecological level. Experimental data from micro- and mesocosms provide estimates of community effect levels, which can then be used to confirm or correct the extrapolations from theoretical methods such as species sensitivity distributions (SSDs) or others. When assessing impacts, the choice of sensitive community endpoints is important. Four community endpoints (species richness, abundance, diversity and similarity indices) were evaluated in their ability to assess impacts of two insecticides, imidacloprid and etofenprox, and their mixture on aquatic and benthic communities from artificial rice paddies. Proportional changes of each community endpoint were expressed by ratios between their values in the treatment and control paddies. Regression lines fitted to the endpoint ratios against the time series of chemical concentrations were used to predict percentile impacts in the communities. The abundance endpoint appears to be the most sensitive indicator of the communities' response, but the Czekanowski similarity index described best the structural changes that occur in all communities. Aquatic arthropods were more sensitive to the mixture of both insecticides than zooplankton and benthic communities. Estimated protective levels for 95% of aquatic species exposed to imidacloprid (<0.01-1.0 µg l(-1)) were slightly lower than predicted by SSD, whereas for etofenprox the protective concentrations in water (<0.01-0.58 µg l(-1)) were an order of magnitude lower than SSD's predictions.
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Ecosistema , Monitoreo del Ambiente/métodos , Contaminantes Ambientales/toxicidad , Oryza , Xenobióticos/toxicidad , Agricultura , Grupos de Población Animal/fisiología , Animales , Biodiversidad , Determinación de Punto Final , Imidazoles/toxicidad , Insecticidas/toxicidad , Neonicotinoides , Nitrocompuestos/toxicidad , Dinámica Poblacional , Crecimiento Demográfico , Piretrinas/toxicidad , Contaminantes Químicos del Agua/toxicidadRESUMEN
The environmental risks of pesticides are typically determined by laboratory single-species tests based on OECD test guidelines, even if biodiversity should also be taken into consideration. To evaluate how realistic these assessments are, ecological changes caused by the systemic insecticides imidacloprid and fipronil, which have different physicochemical properties, when applied at recommended commercial rates on rice fields were monitored using experimental paddy mesocosms. A total of 178 species were observed. There were no significant differences in abundance of crop arthropods among the experimental paddies. However, zooplankton, benthic and neuston communities in imidacloprid-treated field had significantly less abundance of species than control and fipronil fields. Significant differences in abundance of nekton community were also found between both insecticide-treated paddies and control. Influences on the growth of medaka fish were also found in both adults and their fries. Both Principal Response Curve analysis (PRC) and Detrended Correspondence Analysis (DCA) showed the time series variations in community structure among treatments, in particular for imidacloprid during the middle stage of the experimental period. These results show the ecological effect-concentrations (LOEC ~ 1 µg/l) of these insecticides in mesocosms, especially imidacloprid, are clearly different from their laboratory tests. We suggest that differences in the duration of the recovery process among groups of species are due to different physicochemical properties of the insecticides. Therefore, realistic prediction and assessment of pesticide effects at the community level should consider not only the sensitivity traits and interaction among species but also the differences in physicochemical characteristics of each pesticide.
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Biota , Monitoreo del Ambiente/métodos , Insecticidas/análisis , Oryza/crecimiento & desarrollo , Contaminantes Químicos del Agua/análisis , Animales , Artrópodos/efectos de los fármacos , Fenómenos Químicos , Ecosistema , Imidazoles/análisis , Imidazoles/toxicidad , Análisis Multivariante , Neonicotinoides , Nitrocompuestos/análisis , Nitrocompuestos/toxicidad , Pirazoles/análisis , Pirazoles/toxicidad , Medición de Riesgo , Contaminantes Químicos del Agua/toxicidadRESUMEN
Differences in susceptibility of five cladocerans to the neonicotinoid imidacloprid and the phenyl-pyrazole fipronil, which have been dominantly used in rice fields of Japan in recent years, were examined based on short-term (48-h), semi-static acute immobilization exposure tests. Additionally, we compared the species sensitivity distribution (SSD) patterns of both insecticides between two sets of species: the five tested cladocerans and all other aquatic organisms tested so far, using data from the ECOTOX database of U.S. Environmental Protection Agency (USEPA). The sensitivity of the test species to either imidacloprid or fipronil was consistent, spanning similar orders of magnitude (100 times). At the genus level, sensitivities to both insecticides were in the following descending order: Ceriodaphnia > Moina > Daphnia. A positive relationship was found between body lengths of each species and the acute toxicity (EC(50)) of the insecticides, in particular fipronil. Differences in SSD patterns of imidacloprid were found between the species groups compared, indicating that test cladocerans are much less susceptible than other aquatic species including amphibians, crustaceans, fish, insects, mollusks and worms. However, the SSD patterns for fipronil indicate no difference in sensitivity between cladocerans tested and other aquatic organisms despite the greater exposure, which overestimates the results, of our semi-static tests. From these results, Ceriodaphnia sp. should be considered as more sensitive bioindicators (instead of the standard Daphnia magna) for ecotoxicological assessments of aquatic ecosystems. In addition, we propose that ecotoxicity data associated with differences in susceptibility among species should be investigated whenever pesticides have different physicochemical properties and mode of action.
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Cladóceros/efectos de los fármacos , Imidazoles/toxicidad , Insecticidas/toxicidad , Nitrocompuestos/toxicidad , Pirazoles/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Cladóceros/fisiología , Bases de Datos Factuales , Dosificación Letal Mediana , Neonicotinoides , Especificidad de la Especie , Pruebas de Toxicidad Aguda , Estados Unidos , United States Environmental Protection Agency , ZooplanctonRESUMEN
Pesticides released to the environment can indirectly affect target and non-target species in ways that are often contrary to their intended use. Such indirect effects are mediated through direct impacts on other species or the physical environment and depend on ecological mechanisms and species interactions. Typical mechanisms are the release of herbivores from predation and release from competition among species with similar niches. Application of insecticides to agriculture often results in subsequent pest outbreaks due to the elimination of natural enemies. The loss of floristic diversity and food resources that result from herbicide applications can reduce populations of pollinators and natural enemies of crop pests. In aquatic ecosystems, insecticides and fungicides often induce algae blooms as the chemicals reduce grazing by zooplankton and benthic herbivores. Increases in periphyton biomass typically result in the replacement of arthropods with more tolerant species such as snails, worms and tadpoles. Fungicides and systemic insecticides also reduce nutrient recycling by impairing the ability of detritivorous arthropods. Residues of herbicides can reduce the biomass of macrophytes in ponds and wetlands, indirectly affecting the protection and breeding of predatory insects in that environment. The direct impacts of pesticides in the environment are therefore either amplified or compensated by their indirect effects.
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With the exponential number of published data on neonicotinoids and fipronil during the last decade, an updated review of literature has been conducted in three parts. The present part focuses on gaps of knowledge that have been addressed after publication of the Worldwide Integrated Assessment (WIA) on systemic insecticides in 2015. More specifically, new data on the mode of action and metabolism of neonicotinoids and fipronil, and their toxicity to invertebrates and vertebrates, were obtained. We included the newly detected synergistic effects and/or interactions of these systemic insecticides with other insecticides, fungicides, herbicides, adjuvants, honeybee viruses, and parasites of honeybees. New studies have also investigated the contamination of all environmental compartments (air and dust, soil, water, sediments, and plants) as well as bees and apicultural products, food and beverages, and the exposure of invertebrates and vertebrates to such contaminants. Finally, we review new publications on remediation of neonicotinoids and fipronil, especially in water systems. Conclusions of the previous WIA in 2015 are reinforced; neonicotinoids and fipronil represent a major threat worldwide for biodiversity, ecosystems, and all the services the latter provide.
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Insecticidas , Animales , Abejas , Ecosistema , Insecticidas/análisis , Invertebrados , Neonicotinoides , SueloRESUMEN
Over-reliance on pesticides for pest control is inflicting serious damage to the environmental services that underpin agricultural productivity. The widespread use of systemic insecticides, neonicotinoids, and the phenylpyrazole fipronil in particular is assessed here in terms of their actual use in pest management, effects on crop yields, and the development of pest resistance to these compounds in many crops after two decades of usage. Resistance can only be overcome in the longterm by implementing methods that are not exclusively based on synthetic pesticides. A diverse range of pest management tactics is already available, all of which can achieve efficient pest control below the economic injury level while maintaining the productivity of the crops. A novel insurance method against crop failure is shown here as an example of alternative methods that can protect farmer's crops and their livelihoods without having to use insecticides. Finally, some concluding remarks about the need for a new framework for a truly sustainable agriculture that relies mainly on natural ecosystem services instead of chemicals are included; this reinforcing the previous WIA conclusions (van der Sluijs et al. Environ Sci Pollut Res 22:148-154, 2015).
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Insecticidas , Plaguicidas , Ecosistema , Neonicotinoides , Control de PlagasRESUMEN
Synthetic pesticides such as neonicotinoids are commonly used to treat crops in tropical regions, where data on environmental and human contamination are patchy and make it difficult to assess to what extent pesticides may harm human health, especially in less developed countries. To assess the degree of environmental and human contamination with neonicotinoids we collected soil, water and people's hair in three agricultural regions of the Philippines and analysed them by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS-MS). Five neonicotinoids, namely acetamiprid, clothianidin, imidacloprid, thiacloprid and thiamethoxam were targeted. Residues of neonicotinoids were found in 78% of 67 soil samples from the three provinces. Total neonicotinoid loads ranged on average between 0.017 and 0.89 µg/kg in soils of rice, banana and vegetable crops, and were 130 times higher (113.5 µg/kg) in soils of a citrus grove. Imidacloprid was the most prevalent compound at an average of 0.56 µg/kg in soil while thiacloprid was below the limit of detection. Half of the eight water samples from a rice field and nearby creek contained residues of imidacloprid (mean 1.29 ng/L) and one contained thiamethoxam (0.15 ng/L). Residues of neonicotinoids were found in 81% of 99 samples of people's hair from the surveyed regions (average total concentrations 0.14 to 1.18 ng/g, maximum 350 ng/g). Hair residue levels correlated well with the concentrations of thiamethoxam and total residues in soils from the same locality (r = 0.98). The presence of thiacloprid in 15% of the hair samples but not in soil samples suggests an additional route of exposure among people, which is most likely to be through ingestion of agricultural food and drinks available in the market.
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Insecticidas , Suelo , Humanos , Insecticidas/análisis , Neonicotinoides , Nitrocompuestos , Filipinas , AguaRESUMEN
New information on the lethal and sublethal effects of neonicotinoids and fipronil on organisms is presented in this review, complementing the previous Worldwide Integrated Assessment (WIA) in 2015. The high toxicity of these systemic insecticides to invertebrates has been confirmed and expanded to include more species and compounds. Most of the recent research has focused on bees and the sublethal and ecological impacts these insecticides have on pollinators. Toxic effects on other invertebrate taxa also covered predatory and parasitoid natural enemies and aquatic arthropods. Little new information has been gathered on soil organisms. The impact on marine and coastal ecosystems is still largely uncharted. The chronic lethality of neonicotinoids to insects and crustaceans, and the strengthened evidence that these chemicals also impair the immune system and reproduction, highlights the dangers of this particular insecticidal class (neonicotinoids and fipronil), with the potential to greatly decrease populations of arthropods in both terrestrial and aquatic environments. Sublethal effects on fish, reptiles, frogs, birds, and mammals are also reported, showing a better understanding of the mechanisms of toxicity of these insecticides in vertebrates and their deleterious impacts on growth, reproduction, and neurobehaviour of most of the species tested. This review concludes with a summary of impacts on the ecosystem services and functioning, particularly on pollination, soil biota, and aquatic invertebrate communities, thus reinforcing the previous WIA conclusions (van der Sluijs et al. 2015).
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Insecticidas , Animales , Abejas , Ecosistema , Insecticidas/análisis , Insecticidas/toxicidad , Invertebrados , Neonicotinoides , Nitrocompuestos , PolinizaciónRESUMEN
Our mechanistic understanding of the toxicity of chemicals that target biochemical and/or physiological pathways, such as pesticides and medical drugs is that they do so by binding to specific molecules. The nature of the latter molecules (e.g., enzymes, receptors, DNA, proteins, etc.) and the strength of the binding to such chemicals elicit a toxic effect in organisms, which magnitude depends on the doses exposed in a given timeframe. While dose and time of exposure are critical factors determining the toxicity of pesticides, different types of chemicals behave differently. Experimental evidence demonstrates that the toxicity of neonicotinoids increases with exposure time as much as with the dose, and therefore it has been described as time-cumulative toxicity. Examples for aquatic and terrestrial organisms are shown here. This pattern of toxicity, also found among carcinogenic compounds and other toxicants, has been ignored in ecotoxicology and risk assessments for a long time. The implications of the time-cumulative toxicity of neonicotinoids on non-target organisms of aquatic and terrestrial environments are far reaching. Firstly, neonicotinoids are incompatible with integrated pest management (IPM) approaches and secondly regulatory assessments for this class of compounds cannot be based solely on exposure doses but need also to take into consideration the time factor.
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Insecticidas , Plaguicidas , Medición de Riesgo , Ecotoxicología , Sustancias Peligrosas , Insecticidas/toxicidad , Neonicotinoides , Plaguicidas/toxicidadRESUMEN
Food can be health-giving. A global transition towards plant-based diets may equally help curb carbon emissions, slow land-system change and conserve finite resources. Yet, projected benefits of such 'planetary health' diets imperfectly capture the environmental or societal health outcomes tied to food production. Here, we examine pesticide-related hazards of fruit and vegetable consumption, and list proven management alternatives per commodity, geography and chemical compound. Across countries, pesticide use in these alleged healthful foods is extensive with up to 97% food items containing residues and up to 42% posing dietary risks to consumers. Multiple residues are present in 70-92% of US- and China-grown stone fruit while 58% US cauliflower is tainted with neonicotinoid insecticides. Science-based alternatives and decision-support frameworks can help food producers reduce risks and potential harm by deliberately abstaining from pesticide use. As such, opportunities abound to advance 'win-win' diets that simultaneously nurture human health and conserve global biodiversity.
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Residuos de Plaguicidas , Verduras , China , Dieta , Dieta Vegetariana , Salud Ambiental , Humanos , Residuos de Plaguicidas/análisisRESUMEN
The influence of various diets on the survival, fecundity, and the polyunsaturated fatty acid (PUFA) composition of the benthic estuarine amphipod Melita plumulosa (Zeidler) in laboratory cultures were determined. Apart from a natural silty sediment, six commercial food supplements were examined: an omega-6 PUFA enriched Spirulina-based dry powder, Sera micron; a shrimp-based pellet food; an omega-3 PUFA enriched algal paste, Rotiselco-ALG; an omega-6 PUFA enriched algal dry powder, AlgaMac-ARA (arachidonic acid); flaxseed meal; and an omega-3 PUFA enriched dry powder, Frippak. We have previously established that M. plumulosa cultures perform poorly and eventually decline if provided with silty sediment alone, but will thrive if supplemented with Sera micron. Conversely, if the amphipods are cultured on a nutrient-depleted sand substrate, Sera micron alone does not constitute an adequate feed. The major difference in the fatty acid composition of M. plumulosa cultured on silty sediment compared to amphipods cultured on a sand substrate and both fed Sera micron was an increase in the ratio of omega-3 to omega-6 PUFAs, indicating that the silty sediment provides additional food sources rich in omega-3 PUFAs. Furthermore, amphipods cultured in sand and fed any of the three algal-based foods or the Frippak powder as the sole food source had poor survival rates, although Sera micron maintained the best survival-this was attributed to it containing high amounts of beta-carotene and terpenoids. Melita plumulosa fed a mixture of Sera micron in conjunction with the omega-3 PUFA enriched Rotiselco-ALG and cultured on a silty substrate were found to have good fecundity with low variability.
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Anfípodos/química , Dieta , Ácidos Grasos/análisis , Fertilidad , Anfípodos/fisiología , Animales , Carotenoides/análisis , Cromatografía de Gases , Cromatografía Liquida , Esterificación , Sedimentos Geológicos , Espectrometría de MasasRESUMEN
In the Coleambally irrigation area (NSW, Australia), the occurrence of four tadpole and frog species in rice bays on farms growing either rice only or both rice and corn was studied over two seasons. In addition to analysis of species occurrence, both gonadal histology and assessment of Batrachochytrium dendrobatidis infection rates were performed. The rice acreage available as potential tadpole habitat was extensively distributed throughout the irrigation area, but more corn was grown in the northern region compared with the southern region. The mean abundance of Litoria raniformis tadpoles was significantly lower in the northern sites compared with the southern sites. In contrast, tadpoles of Limnodynastes fletcheri, Limnodynastes tasmaniensis, and Crinia parinsignifera had a uniform distribution across all study sites. A principal components analysis showed a relationship between farm type and the rice herbicide applied when the crops were initially sown, with sites occupied by Litoria raniformis in the beginning being predominantly rice-only farms. A discriminant analysis showed that low concentrations of the corn herbicide metolachlor and increased pH were the main variables studied that determined site occupation by L. raniformis. This suggested that farms growing only rice (and not corn) with high algal production were the preferred sites. The rates of chytrid infection and gonadal malformations were low across both regions. Histology of the gonads of metamorphs showed that L. raniformis gonadal differentiation is slow compared to that of the two Limnodynastes species. We concluded that farm practices associated with increased corn cropping in the northern region, rather than any direct effect of corn herbicides, determine the reduced presence of Litoria raniformis in the northern region.
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Productos Agrícolas , Oryza , Plaguicidas/toxicidad , Ranidae/clasificación , Animales , Femenino , Masculino , Razón de Masculinidad , Especificidad de la EspecieRESUMEN
The ability to predict the effects of toxicants in organisms with reasonable accuracy depends to a great extent on the toxico-kinetic models used to describe such effects. Toxic effects of organic chemicals and heavy metals have been described adequately using a hyperbolic model that considers the concentration of the toxicant and the time of exposure only. Such a model relies on the median time to effect (ET(50)) of a chemical to estimate effects at any exposure time, but cannot make predictions for concentrations other than those tested experimentally. A complementary log-to-log model can calculate all ET(50) values for a toxicant, thus enabling the hyperbolic model to predict any level of effect for any combination of concentrations and times of exposure. The parameter values used in both models are obtained from experimental bioassays where the time-to-effect of a toxicant is recorded regularly in addition to standard acute or chronic toxicity data. These models will facilitate the risk assessment of chemicals by (1) predicting effects under any combination of time and concentrations, and (2) reducing to a minimum the experimental efforts required to obtain comprehensive ecotoxicity data.