Evaluation of DNA damage and lipoperoxidation of propofol in patients undergoing elective surgery.

BACKGROUND AND OBJECTIVES: Inhaled anaesthetics have been studied regarding their genotoxic and mutagenic potential in vivo. Propofol differs from volatile anaesthetics because it does not show mutagenic effects and it has been reported to be an antioxidant. However, there are no studies with propofol and genotoxicity in vivo. The study aimed to evaluate the hypothesis that propofol is not genotoxic and it inhibits lipid peroxidation [malondialdehyde (MDA)] in patients undergoing propofol anaesthesia. METHODS: ASA physical status I patients scheduled for elective surgery, lasting at least 90 min, were enrolled in this study. Initially, the estimated plasma concentration of propofol was targeted at 4 microg ml(-1) and then maintained at 2-4 microg ml(-1) until the end of surgery. Haemodynamic data were determined at baseline (before premedication) and in conjunction with target-controlled infusion of propofol: after tracheal intubation, 30, 60 and 90 min after anaesthesia induction and at the end of the surgery. Venous blood samples were collected at baseline, after tracheal intubation, at the end of the surgery and on the postoperative first day for evaluating DNA damage in white blood cells (WBCs), by comet assay, and MDA levels. RESULTS: Haemodynamic data did not differ among times. No statistically significant differences were observed for the levels of DNA damage in WBCs, nor in plasma MDA, among the four times. CONCLUSION: Propofol does not induce DNA damage in WBCs and does not alter MDA in plasma of patients.

A Eletromiografia na avaliação do tratamento de maloclusões com Ortopedia Funcional dos Maxilares / Electromyography evaluation in malocclusion treatment with Functional Maxillary Orthopedics

O objetivo deste trabalho foi verificar a validade na utilização da Eletromiografia como ferramenta de diagnóstico e avaliação dos tratamentos com Ortopedia Funcional dos Maxilares, em pacientes portadores de maloclusões.. Foram selecionados 19 voluntários de ambos os sexos, que foram submetidos à avaliação eletromiográfica bilateral dos músculos temporal porção anterior, masseter e supra-hioideos, em repouso, oito minutos após instalação do aparelho ortopédico funcional, e quando a relação de incisivos em D.A. foi constatada. Os dados eletromiográficos foram coletados utilizando o módulo condicionador de sinais com oito canais, modelo EMG 1000, e analisados com o Software AqDAnalysis 4,18 da Lynx Electronics Ltda.. Os tratamentos foram efetivados com aparelhos ortopédicos funcionais bioplásticos e bioelásticos. Os resultados permitiram concluir pela validade da aplicação da Eletromiografia tanto no diagnóstico, como na avaliação dos estados referentes ao sistema neuromuscular resultantes de tratamentos de maloclusões com a Ortopedia Funcional dos Maxilares.

The main objective of this work was to verify the validity of Electromyography use as a tool for diagnosis and evaluation of Functional Maxillary Orthopedics malocclusion treatments. Nineteen individuals were selected from both sexes, and submitted to bilateral electromyographic evaluation from anterior temporalis, masseter and suprahyoids muscles in rest, eight minutes after functional orthopedic appliance installation and when D.A. incisors relationship were gained. The electromyographic data were collected using signal conditioner module with eight channels, EMG 1000 model, and analyzed with AqDAnalysis 4,18 from Lynx Electronics Ltd. The malocclusion treatments were done with bioplastics and bioelastics functional orthopedic appliances. The results permit concludes that Electromyography is a trustable tool to be used in Diagnosis and in evaluation of neuromuscular system results from malocclusions treatments with Functional Maxillary Orthopedics.