RESUMEN
The SINERGIE (South Italian Network for Rational Guidelines and International Epidemiology) project is intended to set up a collaborative network comprising virologists, clinicians and public health officials dealing with patients affected by HCV disease in the Calabria Region. A prospective observational data-base of HCV infection will be developed and used for studies on HCV natural history, response to treatment, pharmaco-economics, disease complications, and HCV epidemiology (including phylogenetic analysis). With this approach, we aim at improving the identification and care of patients, focusing on upcoming research questions. The final objective is to assist in improving care delivery and inform Public Health Authorities on how to optimize resource allocation in this area.
Asunto(s)
Hepatitis C/epidemiología , Hepatitis C/prevención & control , Bases de Datos Factuales , Directrices para la Planificación en Salud , Hepatitis C/tratamiento farmacológico , Humanos , Italia/epidemiología , Salud PúblicaRESUMEN
The parameters in antibody-positive with LAS/ARC and antibody negative drug-users have been studied. The results show that in the first group the lymphocyte profile and in vitro immunoglobulin production are greatly affected. In the second group only modifications about activation markers and PWM induced B lymphocyte differentiation are present.
Asunto(s)
Complejo Relacionado con el SIDA/inmunología , Formación de Anticuerpos , Seropositividad para VIH/inmunología , Activación de Linfocitos , Subgrupos Linfocitarios , Abuso de Sustancias por Vía Intravenosa/inmunología , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Diferenciación Celular , Células Cultivadas , Humanos , Inmunofenotipificación , Activación de Linfocitos/efectos de los fármacos , Mitógenos de Phytolacca americana/farmacología , Receptores de Interleucina-2/análisis , Receptores de Transferrina/análisisRESUMEN
In this study we have investigated the role of PGE2 in the activation of human T lymphocytes by PWM. A preincubation of these cells with molar concentrations of the prostaglandin ranging from 10(-9) M to 10(-4) M is able to reduce the expression of IL-2R and CD71 on T lymphocyte membrane during the first days of culture, while the DR molecule which is expressed later in the same experimental conditions is not affected by the treatment of T lymphocytes with PGE2. The PGE2-induced inhibition of IL-2R and CD71 well correlates with the reduction of 3H-thymidine incorporation by T cells, indicating that a preincubation of T lymphocytes with PGE2 profoundly affects the proliferative apparatus of these cells when they are stimulated by PWM.