The adverse effects of mining pollutants on oral mucosa in Bellary district: A clinical and genotoxicity study.

Background: The Bellary district in Karnataka, rich in mineral resources, is a major mining industry, but prolonged exposure to mining can lead to health hazards. The study aims to assess the genotoxic impact of mining pollutants on mine workers using the micro-nucleus (MN) assay. Setting and Design: Cross-sectional study. Materials and Methods: A total of 250 individuals (198 males and 52 females) working in mining areas were examined, and their oral findings were recorded in a proforma. For the micro-nucleus assay, buccal smears from 30 individuals working in mining areas with habits, 30 individuals working in mining areas without habits, and 30 individuals residing in non-mining areas (control group) were selected. Smears were stained with Giemsa stain to identity and quantify the MNs. Results: The frequency of oral mucosal problems among 250 persons working in mining regions was 170 (68.0%) with no oral mucosal conditions, 79 (32.6%) with oral mucosal conditions, 25 (10%) with leukoplakia, 1 (0.4%) with lichen planus, and 8 (3.2%) with ulcerations. Acute necrotising gingivitis was reported in one person (0.4%), candidiasis in two (0.8%), abscess in two (0.8%), OSMF in 39 (15.6%), and oral cancer in two (0.8%). The mean MN count was 2.40 + 1.57 in mine employees with habits, 2.18 + 1.25 in mine workers without habits, and 1.40 + 0.55 in normal healthy controls. Conclusion: Reduced occupational health risks brought on by exposure to mining contaminants require protective measures. After being exposed to mining pollutants, exfoliated buccal mucosal cells can be examined for genotoxicity.

Plastination - A method for preservation of oral hard and soft tissue biopsy specimen v/s the conventional method of preservation with formalin.

Context: Plastination is one of the most advanced method for preserving perishable biological specimen as well as tissue samples as for a longer period of time using polymers. Aims: To evaluate the changes in dimension of hard and soft tissue specimens after plastination procedure and compare it with the conventional method of preservation in formalin. Settings and Design: Institution-based retrospective study. Methods and Material: The study included 20 formalin-fixed soft tissue and 10 formalin-fixed hard tissue specimens. All the specimens were plastinated which involved four basic steps of fixation, dehydration and defatting, impregnation with polymer and curing of polymer followed by finishing and storage. The specimens were analysed for shrinkage and dimensional changes and changes in colour and consistency between formalin-fixed specimen and plastinated soft tissue and hard tissue specimen. Statistical Analysis Used: Descriptive statistics were used. Results: After plastination, soft tissues showed average shrinkage of 3.49%with a range of 0.80-7.90% in comparison to the original size. In case of teeth and hard tissue specimen, there was no evidence of dimensional changes or shrinkage before and after plastination. Changes in colour and consistency of the soft tissue specimens were also noted before plastination and after plastination. Conclusions: Although the plastinated specimens in the current study showed minimal shrinkage rate, they have proved to be an excellent alternative to formalin-fixed specimens as they are easy to handle and maintain, non-infectious, non-toxic, user-friendly.

Surgical Protocols before and after COVID-19-A Narrative Review.

The COVID-19 epidemic has affected not only people's daily lives but also the working methods of clinicians, surgical procedures, open/minimally invasive procedures, operating room management, patient and healthcare worker safety, education and training. The main objective of this study was to review selected articles and determine the changes in the general surgery protocols/procedures before and after the emergence of the COVID-19 pandemic. The literature was carried out in PubMed-Medline, Cochrane Library, Embase, Scopus and Google Scholar. The terms utilised for the searches were "SARS-CoV-2", "Surgery", "COVID-19", "Surgical protocol", "Surgical recommendations" and "before and after". A total of 236 studies were identified, out of which 41 studies were included for data extraction. Significant changes in all the articles were observed with respect to the surgeries done before, during and after the COVID-19 pandemic. Specifically, the number of elective surgeries were considerably fewer in comparison to the pre-pandemic period. Since the COVID-19 pandemic started, hospitals all throughout the world have conducted significantly fewer procedures, particularly elective/non-urgent surgeries.

Root Canal Morphology of Maxillary First Premolars Using the Clearing Technique in a South Indian Population: An In vitro Study.

BACKGROUND: The success of root canal therapy requires a good knowledge of root canal morphology. Literature review shows variations in a number of roots and canal morphology in studies done across the globe. AIM: The purpose of this study was to evaluate the root canal morphology in maxillary first premolars using the clearing technique in a South Indian population. METHODOLOGY: Two hundred and twenty-five extracted noncarious, nonfractured, sound maxillary first premolars were collected, cleaned, and were injected with India ink to stain the root canals. Teeth were then decalcified and cleared using methyl salicylate. Teeth were then viewed under a stereomicroscope to evaluate the number of roots, root canal morphology based on the Vertucci's classification, variations, and additional features such as accessory root canals, lateral canals, furcation canals, isthmus, and apical delta. RESULTS: Nearly 52.88% of maxillary first premolars were single rooted, 44.88% had two roots, and 2.22% had three roots. Vertucci's Type IV root canal configuration was the most common type in maxillary first premolars and was seen in 66.51% of cases followed by Type II in 13.4%. Type I was seen in 9.76%, Type VI in 6.51%, Type VIII in 2.32%, Type III in 0.46%, Type V in 0.46%, and Type VII in 0% of teeth. CONCLUSION: The majority of the maxillary first premolars included in our study were single rooted and Vertucci's Type IV was the most common type of root canal configuration.

Serum and salivary lactate dehydrogenase levels as biomarkers of tissue damage among cigarette smokers. A biochemical study.

OBJECTIVE: To estimate the serum and salivary lactate dehydrogenase levels in cigarette smokers and non-smokers. MATERIALS AND METHODS: In this study lactate dehydrogenase levels were estimated in 30 healthy individuals with no tobacco related habits and in 30 patients with history of smoking cigarettes for a minimum of 2 years using Spectrophotometry. RESULTS: The mean values for serum and salivary lactate dehydrogenase levels were higher in cigarette smokers when compared to non-smokers. Serum lactate dehydrogenase levels on comparison between the groups was statistically significant (p=0.04). The values of salivary lactate dehydrogenase levels between the groups was highly significant (p<0.001). CONCLUSION: Cigarette smoking leads to an increase in serum as well as salivary Lactate dehydrogenase levels as indicator of tissue damage in the oral cavity. The present study indicates saliva as a better test medium than serum in determination of lactate dehydrogenase levels.

Large Complex Odontoma: A report of a rare entity.

Odontomas are hamartomatous lesions composed of mature enamel, dentin, and pulp, and may be compound or complex depending on the extent of morphodifferentiation or on their resemblance to normal teeth. They are the most common benign odontogenic tumours, constituting 22% of all odontogenic tumours of the jaw. They are often non-aggressive and slow growing in nature, and are usually diagnosed on routine radiological examinations in the second decade of life. We report the case of an unusually large, painless, complex odontoma, which is a rare entity. It was located in the left posterior mandible and was associated with missing 1(st) and 2(nd) left mandibular molars. The diagnosis was confirmed following surgical excision and histopathological analysis of the lesion.