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1.
Zhongguo Zhong Yao Za Zhi ; 32(8): 661-3, 2007 Apr.
Artículo en Zh | MEDLINE | ID: mdl-17608212

RESUMEN

OBJECTIVE: To detect the polymorphisms of Radix Plygoni Multiflori in chongqing by means of a new marker system SRAP. METHOD: Different shaples of Radix Plygoni Multiflori from major production areas were collected. The SRAP was used to asses divergence among 16 populations. The data were analyzed using unweighted pairgroup method, based on arithmetic averages (UPGMA) bootstrap analysis. Cluster analyses was performed by using DPSv3.01 software, the alkaloid was extracted from P. ternate with chlorolform. RESULT: 104 combinations generated 250 polymorphie bands, the cluster analysis indicated that 16 materials could be distinguished into two main groups and one special type, Nei&Li similarity coefficient ranged from 0.23-0.99, and the average distance is 0. 44. CONCLUSION: The results of the study showed a potential application of SRAP fingerprinting for identification of Radix Plygoni Multiflori.


Asunto(s)
ADN de Plantas/genética , Variación Genética , Técnicas de Amplificación de Ácido Nucleico/métodos , Polygonum/genética , Análisis por Conglomerados , Marcadores Genéticos , Filogenia , Raíces de Plantas/genética , Plantas Medicinales/clasificación , Plantas Medicinales/genética , Polygonum/clasificación
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