RESUMEN
BACKGROUND: Magnolia extract (ME) is known to inhibit cancer growth and metastasis in several cell types in vitro and in animal models. However, there is no detailed study on the preventive efficacy of ME for oral cancer, and the key components in ME and their exact mechanisms of action are not clear. The overall goal of this study is to characterize ME preclinically as a potent oral cancer chemopreventive agent and to determine the key components and their molecular mechanism(s) that underlie its chemopreventive efficacy. METHODS: The antitumor efficacy of ME in oral cancer was investigated in a 4-nitroquinoline-1-oxide (4NQO)-induced mouse model and in two oral cancer orthotopic models. The effects of ME on mitochondrial electron transport chain activity and ROS production in mouse oral tumors was also investigated. RESULTS: ME did not cause detectable side effects indicating that it is a promising and safe chemopreventive agent for oral cancer. Three major key active compounds in ME (honokiol, magnolol and 4-O-methylhonokiol) contribute to its chemopreventive effects. ME inhibits mitochondrial respiration at complex I of the electron transport chain, oxidizes peroxiredoxins, activates AMPK, and inhibits STAT3 phosphorylation, resulting in inhibition of the growth and proliferation of oral cancer cells. CONCLUSION: Our data using highly relevant preclinical oral cancer models, which share histopathological features seen in human oral carcinogenesis, suggest a novel signaling and regulatory role for mitochondria-generated superoxide and hydrogen peroxide in suppressing oral cancer cell proliferation, progression, and metastasis. Video abstract.
Asunto(s)
Antineoplásicos Fitogénicos , Compuestos de Bifenilo , Lignanos , Magnolia/química , Neoplasias de la Boca/prevención & control , Extractos Vegetales , Animales , Antineoplásicos Fitogénicos/farmacología , Antineoplásicos Fitogénicos/uso terapéutico , Compuestos de Bifenilo/farmacología , Compuestos de Bifenilo/uso terapéutico , Línea Celular Tumoral , Evaluación Preclínica de Medicamentos , Femenino , Humanos , Lignanos/farmacología , Lignanos/uso terapéutico , Ratones , Ratones Desnudos , Mitocondrias/metabolismo , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Especies Reactivas de OxígenoRESUMEN
Aurea Helianthus (AH), also known as wild confederate rose or golden sunflower, is a curative herb. It has been used as a medicinal material in China due to its anti-inflammatory, immune regulatory, and anti-oxidant activities. However, its melanogenic effect on skin has not been sufficiently investigated. In this study, we tested whether AH has melanogenic inhibitory activities for the development of effective skin whitening agent. The extract showed inhibition of melanin synthesis and reduced the oxidation of 3, 4-dihydroxyphenilalanine (DOPA) to o-dopaquinone. Additionally, AH downregulated the levels of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase related proteins (TRPs), suggesting that AH has inhibitory effects on melanogenesis. Analysis of the components of AH showed that it contained paprazine and trans-N-feruloyltyramine (FA). We confirmed that the effect of AH resulted from paprazine and FA. Therefore, AH might have potential as an effective candidate for skin whitening.
Asunto(s)
Helianthus/química , Melaninas/antagonistas & inhibidores , Extractos Vegetales/farmacología , Tallos de la Planta/química , Preparaciones para Aclaramiento de la Piel/farmacología , Tiramina/análogos & derivados , Animales , Antineoplásicos Fitogénicos/farmacología , Benzoquinonas/química , Línea Celular Tumoral , Ácidos Cumáricos/farmacología , Dihidroxifenilalanina/análogos & derivados , Dihidroxifenilalanina/química , Regulación hacia Abajo , Melaninas/biosíntesis , Melanoma Experimental/patología , Ratones , Factor de Transcripción Asociado a Microftalmía/metabolismo , Monofenol Monooxigenasa/metabolismo , Proteínas de Plantas/metabolismo , Tiramina/farmacología , Tirosina/metabolismoRESUMEN
Hyperlipidaemia is a major cause of atherosclerosis and related CVD and can be prevented with natural substances. Previously, we reported that a novel Bacillus-fermented green tea (FGT) exerts anti-obesity and hypolipidaemic effects. This study further investigated the hypotriglyceridaemic and anti-obesogenic effects of FGT and its underlying mechanisms. FGT effectively inhibited pancreatic lipase activity in vitro (IC50, 0·48 mg/ml) and ameliorated postprandial lipaemia in rats (26 % reduction with 500 mg/kg FGT). In hypertriglyceridaemic hamsters, FGT administration significantly reduced plasma TAG levels. In mice, FGT administration (500 mg/kg) for 2 weeks augmented energy expenditure by 22 % through the induction of plasma serotonin, a neurotransmitter that modulates energy expenditure and mRNA expressions of lipid metabolism genes in peripheral tissues. Analysis of the gut microbiota showed that FGT reduced the proportion of the phylum Firmicutes in hamsters, which could further contribute to its anti-obesity effects. Collectively, these data demonstrate that FGT decreases plasma TAG levels via multiple mechanisms including inhibition of pancreatic lipase, augmentation of energy expenditure, induction of serotonin secretion and alteration of gut microbiota. These results suggest that FGT may be a useful natural agent for preventing hypertriglyceridaemia and obesity.
Asunto(s)
Camellia sinensis , Metabolismo Energético/efectos de los fármacos , Fermentación , Hiperlipidemias/sangre , Hipolipemiantes/farmacología , Lipasa/antagonistas & inhibidores , Extractos Vegetales/farmacología , Animales , Fármacos Antiobesidad/farmacología , Fármacos Antiobesidad/uso terapéutico , Bacillus , Firmicutes , Microbioma Gastrointestinal/efectos de los fármacos , Hiperlipidemias/tratamiento farmacológico , Hipertrigliceridemia/sangre , Hipertrigliceridemia/tratamiento farmacológico , Hipolipemiantes/uso terapéutico , Metabolismo de los Lípidos/efectos de los fármacos , Metabolismo de los Lípidos/genética , Mesocricetus , Ratones , Ratones Endogámicos C57BL , Páncreas/enzimología , Fitoterapia , Extractos Vegetales/metabolismo , Extractos Vegetales/uso terapéutico , ARN Mensajero/metabolismo , Serotonina/sangre , Té , Triglicéridos/sangreRESUMEN
Numerous medications are used to treat hyperpigmentation. However, several reports have indicated that repeated application of some agents, such as rhododendrol (RD), raspberry ketone (RK) and monobenzone (MB), can be toxic to melanocytes. Although these agents had severe side effects in human trials, no current in vitro methods can predict the safety of such drugs. This study assessed the in vitro effects of five depigmentary compounds including leukoderma-inducing agents. In particular, we determined the effects of different concentrations and exposure times of different depigmentary agents on cell viability and melanogenesis in the presence and absence of ultraviolet B (UVB) radiation. Concentrations of RD, RK and MB that inhibit melanogenesis are similar to concentrations that are cytotoxic; however, concentrations of rucinol (RC) and AP736 that inhibit melanogenesis are much lower than concentrations that are cytotoxic. Furthermore, the concentrations that cause toxic effects depend on exposure duration, and prolonged exposure to RD, RK and MB had more cytotoxic effects than prolonged exposure to RC and AP736. The cytotoxic effects of RD and RK appear to be mediated by apoptosis due to increased expression of caspase-3 and caspase-8; UVB radiation increased the cytotoxicity of these agents and also increased caspase activity. Our results indicate that different leukoderma-inducing compounds have different effects on the viability of normal epidermal melanocytes and suggest that the in vitro assay used here can be used to predict whether an investigational compound that induces leukoderma may lead to adverse effects in human trials.
Asunto(s)
Adamantano/análogos & derivados , Benzamidas/química , Butanoles/química , Butanonas/química , Epidermis/efectos de los fármacos , Hidroquinonas/química , Melanocitos/efectos de los fármacos , Pigmentación , Resorcinoles/química , Adamantano/química , Apoptosis , Caspasa 3/metabolismo , Caspasa 8/metabolismo , Línea Celular Tumoral , Supervivencia Celular , Epidermis/metabolismo , Humanos , Melaninas/biosíntesis , Melanocitos/metabolismo , Necrosis , Rayos UltravioletaRESUMEN
The ethanolic extract of the root of Piper methysticum was found to inhibit melanogenesis in MSH-activated B16 melanoma cells. Flavokawains B and C were isolated from this extract based on their anti-melanogenesis activity and found to inhibit melanogenesis with IC50 values of 7.7µM and 6.9µM, respectively. Flavokawain analogs were synthesized through a Claisen-Schmidt condensation of their corresponding acetophenones and benzaldehydes and were evaluated in terms of their tyrosinase inhibitory and anti-melanogenesis activities. Compound 1b was the most potent of these with an IC50 value of 2.3µM in melanogenesis inhibition assays using MSH-activated B16 melanoma cells.
Asunto(s)
Flavonoides/química , Flavonoides/farmacología , Kava/química , Melaninas/antagonistas & inhibidores , Animales , Flavonoides/síntesis química , Humanos , Melaninas/biosíntesis , Melaninas/síntesis química , Melanoma Experimental/tratamiento farmacológico , Ratones , Relación Estructura-ActividadRESUMEN
Leaves from Camellia sienensis are a popular natural source of various beverage worldwide, and contain caffeine and polyphenols derived from catechin analogues. In the current study, caffeine (CAF, 1) and three tea polyphenols including (-)-epigallocatechin 3-O-gallate (EGCg, 2), (-)-gallocatechin 3-O-gallate (GCg, 3), and (-)-epicatechin 3-O-gallate (ECg, 4) were isolated and purified by flow-rate gradient high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:9:1:9, v/v). Two hundred milligrams of acetone-soluble extract from fermented C. sinensis leaves was separated by HPCCC to give 1 (25.4 mg), 2 (16.3 mg), 3 (11.1 mg) and 4 (4.4 mg) with purities over 98%. The structures of 1-4 were elucidated by QTOF-MS, as well as 1H- and 13C-NMR, and the obtained data were compared to the previously reported values.
Asunto(s)
Acetona/química , Cafeína/aislamiento & purificación , Camellia sinensis/química , Hojas de la Planta/química , Polifenoles/aislamiento & purificación , Cafeína/química , Cromatografía Líquida de Alta Presión/métodos , Polifenoles/químicaRESUMEN
3-Alkyl-2-aryl-2-cyclopenten-1-one oxime derivatives (1) were studied as a novel class of inhibitors of tumor necrosis factor α (TNF-α) with regard to synthesis and in vitro SAR inhibition of TNF-α. The in vitro IC50 values of these compounds in rat and human peripheral blood mononuclear cells were at the sub-micromolar level.
Asunto(s)
Ciclopentanos/química , Ciclopentanos/farmacología , Oximas/química , Oximas/farmacología , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Animales , Relación Dosis-Respuesta a Droga , Humanos , Leucocitos Mononucleares/química , Leucocitos Mononucleares/metabolismo , Modelos Moleculares , Estructura Molecular , Ratas , Relación Estructura-Actividad , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
The subcutaneous fat tissue mass gradually decreases with age, and its regulation is a strategy to develop anti-aging compounds to ameliorate the photo-aging of human skin. The adipogenesis of human adipose tissue-mesenchymal stem cells (hAT-MSCs) can be used as a model to discover novel anti-aging compounds. Cinnamomum cassia methanol extracts were identified as adipogenesis-promoting agents by natural product library screening. Cinnamates, the major chemical components of Cinnamomum cassia extracts, promoted adipogenesis in hAT-MSCs. We synthesized kojyl cinnamate ester derivatives to improve the pharmacological activity of cinnamates. Structure-activity studies of kojyl cinnamate derivatives showed that both the α,ß-unsaturated carbonyl ester group and the kojic acid moiety play core roles in promoting adiponectin production during adipogenesis in hAT-MSCs. We conclude that kojyl cinnamate ester derivatives provide novel pharmacophores that can regulate adipogenesis in hAT-MSCs.
Asunto(s)
Adipogénesis/efectos de los fármacos , Adiponectina/metabolismo , Tejido Adiposo/citología , Cinamatos/química , Cinamatos/farmacología , Células Madre Mesenquimatosas/efectos de los fármacos , Células Cultivadas , Ésteres/química , Ésteres/farmacología , Humanos , Células Madre Mesenquimatosas/citología , Pironas/química , Pironas/farmacologíaRESUMEN
Three-dimensional quantitative structure-activity relationship (3D-QSAR) modeling, comparative molecular field analysis (CoMFA), and comparative molecular similarity indices analysis (CoMSIA) of polyhydroxylated N-benzylbenzamide derivatives containing an adamantyl moiety were performed to understand the mechanism of action and structure-activity relationship of these compounds. Contour map analysis indicated that steric contributions of the adamantyl moiety and electrostatic contributions of the hydroxyl group at the 3-position are important in the activity. Activities of the training set and test sets predicted by CoMFA fit well with actual activities, demonstrating that CoMFA, along with the best calculated q(2) value, has the best predictive ability.
Asunto(s)
Benzamidas/química , Melaninas/antagonistas & inhibidores , Relación Estructura-Actividad Cuantitativa , Animales , Benzamidas/farmacología , Línea Celular Tumoral , Células Cultivadas , Melaninas/metabolismo , Melanocitos/efectos de los fármacos , Melanocitos/fisiología , Ratones , Estructura Secundaria de ProteínaRESUMEN
In this study, we aimed to examine the cellular and molecular mechanisms of lancemaside A from Codonopsis lanceolata (Campanulaceae) in the inflammatory responses of monocytes (U937 cells) and macrophages (RAW264.7 cells). Lancemaside A significantly suppressed the inflammatory functions of lipopolysaccharide- (LPS-) treated RAW264.7 cells by suppressing the production of nitric oxide (NO), the expression of the NO-producing enzyme inducible NO synthase (iNOS), the upregulation of the costimulatory molecule CD80, and the morphological changes induced by LPS exposure. In addition, lancemaside A diminished the phagocytic activity of RAW264.7 cells and boosted the neutralizing capacity of these cells when treated with the radical generator sodium nitroprusside (SNP). Interestingly, lancemaside A strongly blocked the adhesion activity of RAW264.7 cells to plastic culture plates, inhibited the cell-cell and cell-fibronectin (FN) adhesion of U937 cells that was triggered by treatment with an anti-ß1-integrin (CD29) antibody and immobilized FN, respectively. By evaluating the activation of various intracellular signaling pathways and the levels of related nuclear transcription factors, lancemaside A was found to block the activation of inhibitor of κB kinase (IKK) and p65/nuclear factor- (NF-) κB. Taken together, our findings strongly suggest that the anti-inflammatory function of lancemaside A is the result of its strong antioxidative and IKK/NF-κB inhibitory activities.
Asunto(s)
Codonopsis/química , Macrófagos/efectos de los fármacos , Monocitos/efectos de los fármacos , Saponinas/química , Animales , Antioxidantes/química , Adhesión Celular , Línea Celular , Supervivencia Celular , Humanos , Quinasa I-kappa B/metabolismo , Inflamación , Integrina beta1/metabolismo , Lipopolisacáridos , Macrófagos/citología , Ratones , Monocitos/citología , Óxido Nítrico/metabolismo , Nitroprusiato/química , Fagocitosis , Células U937RESUMEN
21-O-Angeloyltheasapogenol E3 (ATS-E3) is a triterpenoid saponin recently isolated from the seeds of the tea tree Camellia sinensis (L.) O. Kuntze. ATS-E3 has several beneficial properties including anti-inflammatory, antidiabetic, antiatherosclerotic, and anticancer effects. Unlike other phenolic compounds isolated from tea plants, there are no studies reporting the pharmacological action of ATS-E3. In this study, we therefore aimed to explore the cellular and molecular inhibitory activities of ATS-E3 in macrophage-mediated inflammatory responses. ATS-E3 remarkably diminished cellular responses of macrophages such as FITC-dextran-induced phagocytic uptake, sodium nitroprusside- (SNP-) induced radical generation, and LPS-induced nitric oxide (NO) production. Analysis of its molecular activity showed that this compound significantly suppressed the expression of inducible NO synthase (iNOS), nuclear translocation of nuclear factor- (NF-) κB subunits (p50 and p65), phosphorylation of inhibitor of κB kinase (IKK), and the enzyme activity of AKT1. Taken together, the novel triterpenoid saponin compound ATS-E3 contributes to the beneficial effects of tea plants by exerting anti-inflammatory and antioxidative activities in an AKT/IKK/NF-κB-dependent manner.
Asunto(s)
Antiinflamatorios/farmacología , Macrófagos/efectos de los fármacos , Saponinas/farmacología , Triterpenos/farmacología , Animales , Antiinflamatorios/aislamiento & purificación , Camellia sinensis , Línea Celular , Células HEK293 , Humanos , Inflamación/inmunología , Inflamación/metabolismo , Inflamación/prevención & control , Macrófagos/inmunología , Macrófagos/metabolismo , Ratones , FN-kappa B/metabolismo , Óxido Nítrico/biosíntesis , Saponinas/aislamiento & purificación , Semillas , Transducción de Señal/efectos de los fármacos , Triterpenos/aislamiento & purificaciónRESUMEN
AP736 was identified as an antimelanogenic drug that can be used for the prevention of melasma, freckles, and dark spots in skin by acting as a suppressor of melanin synthesis and tyrosinase expression. Since macrophage-mediated inflammatory responses are critical for skin health, here we investigated the potential anti-inflammatory activity of AP736. The effects of AP736 on various inflammatory events such as nitric oxide (NO)/prostaglandin (PG) E2 production, inflammatory gene expression, phagocytic uptake, and morphological changes were examined in RAW264.7 cells. AP736 was found to strongly inhibit the production of both NO and PGE2 in lipopolysaccharide- (LPS-) treated RAW264.7 cells. In addition, AP736 strongly inhibited both LPS-induced morphological changes and FITC-dextran-induced phagocytic uptake. Furthermore, AP736 also downregulated the expression of multiple inflammatory genes, such as inducible NO synthase (iNOS), cyclooxygenase- (COX-) 2, and interleukin- (IL-) 1ß in LPS-treated RAW264.7 cells. Transcription factor analysis, including upstream signalling events, revealed that both NF-κB and AP-1 were targeted by AP736 via inhibition of the IKK/IκBα and IRAK1/TAK1 pathways. Therefore, our results strongly suggest that AP736 is a potential anti-inflammatory drug due to its suppression of NF-κB-IKK/IκBα and AP-1-IRAK1/TAK1 signalling, which may make AP736 useful for the treatment of macrophage-mediated skin inflammation.
Asunto(s)
Adamantano/análogos & derivados , Antiinflamatorios no Esteroideos/farmacología , Benzamidas/farmacología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , FN-kappa B/antagonistas & inhibidores , Factor de Transcripción AP-1/antagonistas & inhibidores , Adamantano/química , Adamantano/farmacología , Animales , Antiinflamatorios no Esteroideos/química , Benzamidas/química , Línea Celular , Expresión Génica/efectos de los fármacos , Células HEK293 , Humanos , Inflamación/tratamiento farmacológico , Inflamación/genética , Inflamación/inmunología , Interleucina-1beta/genética , Lipopolisacáridos/toxicidad , Macrófagos/inmunología , Melaninas/biosíntesis , Ratones , Estructura Molecular , Óxido Nítrico Sintasa de Tipo II/genética , Transducción de Señal/efectos de los fármacos , Piel/efectos de los fármacos , Piel/inmunología , Piel/metabolismo , Pigmentación de la Piel/efectos de los fármacosRESUMEN
Hyper-pigmentation causes skin darkness and medical disorders, such as post-inflammatory melanoderma and melasma. Therefore, the development of anti-melanogenic agents is important for treating these conditions and for cosmetic production. In our previous paper, we demonstrated that the anti-diabetic drug voglibose, a valiolamine derivative, is a potent anti-melanogenic agent. In addition, we proposed an alternative screening strategy to identify valiolamine derivatives with high skin permeability that act as anti-melanogenic agents when applied topically. In this study, we synthesized several valiolamine derivatives with enhanced lipophilicity and examined their inhibitory effects in a human skin model. N-(2-hydroxycyclohexyl)valiolamine (HV) possesses a stronger inhibitory effect on melanin production than voglibose in a human skin model, suggesting that HV is a more potent anti-melanogenic agent for the skin.
Asunto(s)
Ciclohexanoles/farmacología , Inositol/análogos & derivados , Melaninas/biosíntesis , Melanocitos/efectos de los fármacos , Células Cultivadas , Ciclohexanoles/síntesis química , Humanos , Inositol/síntesis química , Inositol/química , Inositol/farmacología , Melanocitos/metabolismo , Monofenol Monooxigenasa/genética , Monofenol Monooxigenasa/metabolismoRESUMEN
Lutein is a naturally occurring carotenoid with antioxidative, antitumorigenic, antiangiogenic, photoprotective, hepatoprotective, and neuroprotective properties. Although the anti-inflammatory effects of lutein have previously been described, the mechanism of its anti-inflammatory action has not been fully elucidated. Therefore, in the present study, we aimed to investigate the regulatory activity of lutein in the inflammatory responses of skin-derived keratinocytes or macrophages and to elucidate the mechanism of its inhibitory action. Lutein significantly reduced several skin inflammatory responses, including increased expression of interleukin-(IL-) 6 from LPS-treated macrophages, upregulation of cyclooxygenase-(COX-) 2 from interferon- γ /tumor necrosis-factor-(TNF-) α -treated HaCaT cells, and the enhancement of matrix-metallopeptidase-(MMP-) 9 level in UV-irradiated keratinocytes. By evaluating the intracellular signaling pathway and the nuclear transcription factor levels, we determined that lutein inhibited the activation of redox-sensitive AP-1 pathway by suppressing the activation of p38 and c-Jun-N-terminal kinase (JNK). Evaluation of the radical and ROS scavenging activities further revealed that lutein was able to act as a strong anti-oxidant. Taken together, our findings strongly suggest that lutein-mediated AP-1 suppression and anti-inflammatory activity are the result of its strong antioxidative and p38/JNK inhibitory activities. These findings can be applied for the preparation of anti-inflammatory and cosmetic remedies for inflammatory diseases of the skin.
Asunto(s)
Antiinflamatorios/farmacología , Queratinocitos/efectos de los fármacos , Luteína/farmacología , Macrófagos/efectos de los fármacos , Piel/citología , Factor de Transcripción AP-1/metabolismo , Animales , Línea Celular , Ciclooxigenasa 2/metabolismo , Humanos , Interferón gamma/farmacología , Interleucina-6/metabolismo , Queratinocitos/citología , Queratinocitos/metabolismo , Macrófagos/citología , Macrófagos/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factor de Necrosis Tumoral alfa/farmacologíaRESUMEN
Amentoflavone is a biflavonoid compound with antioxidant, anticancer, antibacterial, antiviral, anti-inflammatory, and UV-blocking activities that can be isolated from Torreya nucifera, Biophytum sensitivum, and Selaginella tamariscina. In this study, the molecular mechanism underlying amentoflavone's anti-inflammatory activity was investigated. Amentoflavone dose dependently suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW264.7 cells stimulated with the TLR4 ligand lipopolysaccharide (LPS; derived from Gram-negative bacteria). Amentoflavone suppressed the nuclear translocation of c-Fos, a subunit of activator protein (AP)-1, at 60 min after LPS stimulation and inhibited the activity of purified and immunoprecipitated extracellular signal-regulated kinase (ERK), which mediates c-Fos translocation. In agreement with these results, amentoflavone also suppressed the formation of a molecular complex including ERK and c-Fos. Therefore, our data strongly suggest that amentoflavone's immunopharmacological activities are due to its direct effect on ERK.
Asunto(s)
Biflavonoides/farmacología , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , Extractos Vegetales/farmacología , Taxaceae/metabolismo , Animales , Supervivencia Celular , Cromatografía Líquida de Alta Presión , Dinoprostona/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Células HEK293 , Humanos , Inflamación , Lipopolisacáridos/metabolismo , Macrófagos/metabolismo , Ratones , Óxido Nítrico/metabolismo , Fosforilación , Proteínas Proto-Oncogénicas c-fos/metabolismo , Factor de Transcripción AP-1/metabolismoRESUMEN
Seed oil triacylglycerol (TAG) composition of 32 soybean varieties were determined and compared using ¹H-NMR. The contents of linolenic (Ln), linoleic (L), and oleic (O) ranged from 10.7% to 19.3%, 37.4%-50.1%, and 15.7%-34.1%, respectively. As is evident, linoleic acid was the major fatty acid of soybean oil. Compositional differences among the varieties were observed. Natural oils containing unsaturated groups have been regarded as important nutrient and cosmetic ingredients because of their various biological activities. The TAG profiles of the soy bean oils could be useful for distinguishing the origin of seeds and controlling the quality of soybean oils. To the best of our knowledge, this is the first study in which the TAG composition of various soybean oils has been analyzed using the ¹H-NMR method.
Asunto(s)
Glycine max/química , Espectroscopía de Resonancia Magnética/métodos , Aceites de Plantas/análisis , Semillas/química , Triglicéridos/análisisRESUMEN
A new series of polyhydroxylated N-benzylbenzamide derivatives containing an adamantyl moiety has been synthesized, and the depigmenting and tyrosinase inhibitory activities of the molecules were evaluated. The lipophilic character of the adamantyl moiety appeared to confer greater depigmentation power on the benzamide derivatives as compared to those lacking adamantyl substitution. Molecular modeling was applied in order to elucidate the interactions between ligands and tyrosinase that led to inhibition.
Asunto(s)
Adamantano/análogos & derivados , Adamantano/farmacología , Agaricales/enzimología , Benzamidas/química , Benzamidas/farmacología , Monofenol Monooxigenasa/antagonistas & inhibidores , Pigmentación de la Piel/efectos de los fármacos , Animales , Línea Celular Tumoral , Melaninas/antagonistas & inhibidores , Melaninas/metabolismo , Melanocitos/efectos de los fármacos , Melanocitos/metabolismo , Ratones , Modelos Moleculares , Monofenol Monooxigenasa/metabolismoRESUMEN
We synthesized benzoate ester derivatives of kojic acid with and without adamantane moiety. Benzoate derivatives 2a-e that did not contain an adamantane moiety showed potent tyrosinase inhibitory activities. However, depigmenting activity was not noted in a cell-based assay. Contrasting results were obtained for benzoate derivatives (3a-e) containing an adamantane moiety. Compounds 3a-e showed potent depigmenting activities without tyrosinase inhibitory activities. To the best of our knowledge, this is the first study showing the depigmenting activities of kojic acid derivatives without tyrosinase inhibitory activities.
Asunto(s)
Adamantano/química , Melaninas/antagonistas & inhibidores , Melanocitos/efectos de los fármacos , Pigmentación/efectos de los fármacos , Pironas/química , Agaricales/enzimología , Animales , Bioensayo , Supervivencia Celular/efectos de los fármacos , Ésteres , Melaninas/biosíntesis , Melanocitos/citología , Melanocitos/metabolismo , Ratones , Monofenol Monooxigenasa/antagonistas & inhibidores , Monofenol Monooxigenasa/metabolismo , Pironas/farmacología , Relación Estructura-Actividad , Tirosina/metabolismoRESUMEN
We synthesized cinnamate derivatives of kojic acid for use as depigmenting agents by various esterification methods. The cinnamate of 5-position of kojic acid (6) was obtained by EDC coupling, DCC coupling, acid chloride, and mixed anhydride methods. To obtain the cinnamate of the 2-position of kojic acid (7), we carried out the nucleophilic addition of the potassium salt of cinnamic acid to kojyl chloride. In this reaction, we discovered the occurrence of a side reaction and identified the structure of the side product thus formed. We evaluated the depigmenting activities of both the side product and the cinnamate derivatives of kojic acid. Interestingly, the side product (11) showed more potent depigmenting activity (IC(50)=23.51µM) than compound 7 (IC(50)>100µM) which is the mother compound of the side product. However, it has no tyrosinase inhibitory activity. Compound 6, the cinnamate of 5-position of kojic acid, also showed moderate depigmenting activity (IC(50)=46.64µM) without tyrosinase inhibitory activity. Production of this side product (11) may have originated from the proton exchange between the potassium salt of cinnamic acid and kojyl chloride. We then efficiently reduced the yield of the side product by controlling the equilibrium of the potassium salt of cinnamic acid. The addition of cinnamic acid greatly reduced the amount of the side product produced.
Asunto(s)
Cinamatos/química , Cinamatos/farmacología , Pigmentación/efectos de los fármacos , Pironas/química , Pironas/farmacología , Animales , Línea Celular , Cinamatos/síntesis química , Melaninas/metabolismo , Melanocitos/efectos de los fármacos , Melanocitos/metabolismo , Ratones , Modelos Moleculares , Monofenol Monooxigenasa/antagonistas & inhibidores , Monofenol Monooxigenasa/metabolismo , Pironas/síntesis químicaRESUMEN
Carnosic acid (CA) is a diterpene compound exhibiting antioxidative, anticancer, anti-angiogenic, anti-inflammatory, anti-metabolic disorder, and hepatoprotective and neuroprotective activities. In this study, the effect of CA on various skin inflammatory responses and its inhibitory mechanism were examined. CA strongly suppressed the production of IL-6, IL-8, and MCP-1 from keratinocyte HaCaT cells stimulated with sodium lauryl sulfate (SLS) and retinoic acid (RA). In addition, CA blocked the release of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) from RAW264.7 cells activated by the toll-like receptor (TLR)-2 ligands, Gram-positive bacterium-derived peptidoglycan (PGN) and pam3CSK, and the TLR4 ligand, Gram-negative bacterium-derived lipopolysaccharide (LPS). CA arrested the growth of dermatitis-inducing Gram-positive and Gram-negative microorganisms such Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus aureus. CA also blocked the nuclear translocation of nuclear factor (NF)-κB and its upstream signaling including Syk/Src, phosphoinositide 3-kinase (PI3K), Akt, inhibitor of κBα (IκBα) kinase (IKK), and IκBα for NF-κB activation. Kinase assays revealed that Syk could be direct enzymatic target of CA in its anti-inflammatory action. Therefore, our data strongly suggest the potential of CA as an anti-inflammatory drug against skin inflammatory responses with Src/NF-κB inhibitory properties.