Light-wave-controlled Haldane model in monolayer hexagonal boron nitride.

In recent years, the stacking and twisting of atom-thin structures with matching crystal symmetry has provided a unique way to create new superlattice structures in which new properties emerge1,2. In parallel, control over the temporal characteristics of strong light fields has allowed researchers to manipulate coherent electron transport in such atom-thin structures on sublaser-cycle timescales3,4. Here we demonstrate a tailored light-wave-driven analogue to twisted layer stacking. Tailoring the spatial symmetry of the light waveform to that of the lattice of a hexagonal boron nitride monolayer and then twisting this waveform result in optical control of time-reversal symmetry breaking5 and the realization of the topological Haldane model6 in a laser-dressed two-dimensional insulating crystal. Further, the parameters of the effective Haldane-type Hamiltonian can be controlled by rotating the light waveform, thus enabling ultrafast switching between band structure configurations and allowing unprecedented control over the magnitude, location and curvature of the bandgap. This results in an asymmetric population between complementary quantum valleys that leads to a measurable valley Hall current7, which can be detected by optical harmonic polarimetry. The universality and robustness of our scheme paves the way to valley-selective bandgap engineering on the fly and unlocks the possibility of creating few-femtosecond switches with quantum degrees of freedom.

Valleytronics in bulk MoS2 with a topologic optical field.

The valley degree of freedom1-4 of electrons in materials promises routes towards energy-efficient information storage with enticing prospects for quantum information processing5-7. Current challenges in utilizing valley polarization are symmetry conditions that require monolayer structures8,9 or specific material engineering10-13, non-resonant optical control to avoid energy dissipation and the ability to switch valley polarization at optical speed. We demonstrate all-optical and non-resonant control over valley polarization using bulk MoS2, a centrosymmetric material without Berry curvature at the valleys. Our universal method utilizes spin angular momentum-shaped trefoil optical control pulses14,15 to switch the material's electronic topology and induce valley polarization by transiently breaking time and space inversion symmetry16 through a simple phase rotation. We confirm valley polarization through the transient generation of the second harmonic of a non-collinear optical probe pulse, depending on the trefoil phase rotation. The investigation shows that direct optical control over the valley degree of freedom is not limited to monolayer structures. Indeed, such control is possible for systems with an arbitrary number of layers and for bulk materials. Non-resonant valley control is universal and, at optical speeds, unlocks the possibility of engineering efficient multimaterial valleytronic devices operating on quantum coherent timescales.

Observation of interband Berry phase in laser-driven crystals.

Ever since its discovery1, the notion of the Berry phase has permeated all branches of physics and plays an important part in a variety of quantum phenomena2. However, so far all its realizations have been based on a continuous evolution of the quantum state, following a cyclic path. Here we introduce and demonstrate a conceptually new manifestation of the Berry phase in light-driven crystals, in which the electronic wavefunction accumulates a geometric phase during a discrete evolution between different bands, while preserving the coherence of the process. We experimentally reveal this phase by using a strong laser field to engineer an internal interferometer, induced during less than one cycle of the driving field, which maps the phase onto the emission of higher-order harmonics. Our work provides an opportunity for the study of geometric phases, leading to a variety of observations in light-driven topological phenomena and attosecond solid-state physics.

A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis.

Mitotic chromatin is largely assumed incompatible with transcription due to changes in the transcription machinery and chromosome architecture. However, the mechanisms of mitotic transcriptional inactivation and their interplay with chromosome assembly remain largely unknown. By monitoring ongoing transcription in Drosophila early embryos, we reveal that eviction of nascent mRNAs from mitotic chromatin occurs after substantial chromosome compaction and is not promoted by condensin I. Instead, we show that the timely removal of transcripts from mitotic chromatin is driven by the SNF2 helicase-like protein Lodestar (Lds), identified here as a modulator of sister chromatid cohesion defects. In addition to the eviction of nascent transcripts, we uncover that Lds cooperates with Topoisomerase 2 to ensure efficient sister chromatid resolution and mitotic fidelity. We conclude that the removal of nascent transcripts upon mitotic entry is not a passive consequence of cell cycle progression and/or chromosome compaction but occurs via dedicated mechanisms with functional parallelisms to sister chromatid resolution.

Polycomb group (PcG) proteins prevent the assembly of abnormal synaptonemal complex structures during meiosis.

The synaptonemal complex (SC) is a proteinaceous scaffold that is assembled between paired homologous chromosomes during the onset of meiosis. Timely expression of SC coding genes is essential for SC assembly and successful meiosis. However, SC components have an intrinsic tendency to self-organize into abnormal repetitive structures, which are not assembled between the paired homologs and whose formation is potentially deleterious for meiosis and gametogenesis. This creates an interesting conundrum, where SC genes need to be robustly expressed during meiosis, but their expression must be carefully regulated to prevent the formation of anomalous SC structures. In this manuscript, we show that the Polycomb group protein Sfmbt, the Drosophila ortholog of human MBTD1 and L3MBTL2, is required to avoid excessive expression of SC genes during prophase I. Although SC assembly is normal after Sfmbt depletion, SC disassembly is abnormal with the formation of multiple synaptonemal complexes (polycomplexes) within the oocyte. Overexpression of the SC gene corona and depletion of other Polycomb group proteins are similarly associated with polycomplex formation during SC disassembly. These polycomplexes are highly dynamic and have a well-defined periodic structure. Further confirming the importance of Sfmbt, germ line depletion of this protein is associated with significant metaphase I defects and a reduction in female fertility. Since transcription of SC genes mostly occurs during early prophase I, our results suggest a role of Sfmbt and other Polycomb group proteins in downregulating the expression of these and other early prophase I genes during later stages of meiosis.

Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors.

Proteases play a pivotal role in several biological processes, from digestion, cell proliferation, and differentiation to fertility. Deregulation of protease metabolism can result in several pathological conditions (i.e., cancer, neurodegenerative disorders, and others). Therefore, monitoring proteolytic activity in real time could have a fundamental role in the early diagnosis of these diseases. Herein, the main approaches used to develop biosensors for monitoring proteolytic activity are reviewed. A comparison of the advantages and disadvantages of each approach is provided along with a discussion of their importance and promising opportunities for the early diagnosis of severe diseases. This new era of biosensors can be characterized by the ability to control and monitor biological processes, ultimately improving the potential of personalized medicine.

A Review on 3D Scanners Studies for Producing Customized Orthoses.

When a limb suffers a fracture, rupture, or dislocation, it is traditionally immobilized with plaster. This may induce discomfort in the patient, as well as excessive itching and sweating, which creates the growth of bacteria, leading to an unhygienic environment and difficulty in keeping the injury clean during treatment. Furthermore, if the plaster remains for a long period, it may cause lesions in the joints and ligaments. To overcome all of these disadvantages, orthoses have emerged as important medical devices to help patients in rehabilitation, as well as for self-care of deficiencies in clinics and daily life. Traditionally, these devices are produced manually, which is a time-consuming and error-prone method. From another point of view, it is possible to use imageology (X-ray or computed tomography) to scan the human body; a process that may help orthoses manufacturing but which induces radiation to the patient. To overcome this great disadvantage, several types of 3D scanners, without any kind of radiation, have emerged. This article describes the use of various types of scanners capable of digitizing the human body to produce custom orthoses. Studies have shown that photogrammetry is the most used and most suitable 3D scanner for the acquisition of the human body in 3D. With this evolution of technology, it is possible to decrease the scanning time and it will be possible to introduce this technology into clinical environment.

Genetic landscape of homologous recombination repair genes in early-onset/familial prostate cancer patients.

Prostate cancer (PrCa) is one of the three most frequent and deadliest cancers worldwide. The discovery of PARP inhibitors for the treatment of tumors with deleterious variants in homologous recombination repair (HRR) genes has placed PrCa on the roadmap of precision medicine. However, the overall contribution of HRR genes to the 10%-20% of carcinomas arising in men with early-onset/familial PrCa has not been fully clarified. We used targeted next-generation sequencing (T-NGS) covering eight HRR genes (ATM, BRCA1, BRCA2, BRIP1, CHEK2, NBN, PALB2, and RAD51C) and an analysis pipeline querying both small and large genomic variations to clarify their global and relative contribution to hereditary PrCa predisposition in a series of 462 early-onset/familial PrCa cases. Deleterious variants were found in 3.9% of the patients, with CHEK2 and ATM being the most frequently mutated genes (38.9% and 22.2% of the carriers, respectively), followed by PALB2 and NBN (11.1% of the carriers, each), and finally by BRCA2, RAD51C, and BRIP1 (5.6% of the carriers, each). Using the same NGS data, exonic rearrangements were found in two patients, one pathogenic in BRCA2 and one of unknown significance in BRCA1. These results contribute to clarify the genetic heterogeneity that underlies PrCa predisposition in the early-onset and familial disease, respectively.

Testing variations between starters and substitute players in terms of total distance, high-speed running, and sprinting distance: a descriptive study on professional male soccer players.

The purpose of this study was three-fold: (i) to compare total distance, high-speed running (HSR) distance, and sprint distance covered per 5-minute epoch by players acting as both starters and substitutes; (ii) to compare the locomotor demands between the moments the players entered the match (45-60, 60-75 and 75-90 minutes); and (iii) to compare the locomotor demands of the players between the variations of the within- and between-playing positions. Twenty-one male professional soccer players competing in the Professional Premier League of one of the European countries were observed over sixteen official matches. The players were monitored during all matches using a Global Navigation Satellite System. The measures collected were total distance (TD; m), distance in HSR, sprint distance, HSR, and sprint counts. Considering the comparisons between the splits over the second half of match play, a significant difference between the starters and the substitutes was observed only for sprint distance in the 90-95 minute split (Z = -2.023; p = 0.043). Moreover, no substantial differences were found between the moment the substitute player entered the match regarding total distance (H = 2.650; p = 0.266), HSR distance (H = 1.738; p = 0.419), and sprint distance (H = 0.048; p = 0.976). However, the comparison of between-playing positions revealed considerable differences in total distance (H = 29.246; p < 0.001), and HSR distance (H = 12.153; p = 0.002) covered by the players acting as starters. In contrast, for substitute players, such differences were reported in HSR distance (H = 27.892; p < 0.001) and sprint distance (H = 15.879; p < 0.001). In conclusion, this study suggests that acting as a starter or a substitute does not significantly affect the intensity of effort except during the last periods of match play. However, the contextual factor of performing in a specific playing position plays a significant role both for starters and substitutes.

Contemporary practices of Portuguese and Brazilian soccer coaches in designing and applying small-sided games.

This descriptive study aimed to investigate the current practices of Portuguese and Brazilian soccer coaches in the design and implementation of small-sided games (SSGs) in soccer. A total of 187 male coaches participated in the online survey, consisting of 82 Portuguese and 105 Brazilian individuals. These coaches held various positions within the technical staff, with 63 serving as head coaches, 38 as assistant coaches, 38 as physical trainers, and 48 in other roles related to the technical staff. Additionally, the participants represented both youth (n = 102) and adult competitive levels (n = 59), along with some who were not currently associated with a specific group. The survey consisted of 32 questions divided into three main sections: (i) the timing of SSG application, (ii) the methods used for applying SSGs, and (iii) the reasons for applying SSGs. The Chi-square test revealed a statistically significant association between nationality and the frequency of SSGs used in training sessions during the pre-season (p = 0.039) and in-season (p < 0.001). Moreover, significant association between nationality and the time allocated to employing SSGs for targeting aerobic training (p < 0.001) was found. There was a significant association between nationality and the weekly frequency of SSGs use for targeting sprint training (p = 0.019). The Chi-square test identified significant associations between nationality and the use of SSGs for targeting technical training (p = 0.002), as well as for tactical training (p = 0.002). In summary, this study underscores that SSGs are primarily employed to enhance aerobic fitness, change of direction, technical skills, and tactical behaviors. Coaches generally favor employing SSGs two to three times a week, with typical sessions lasting between 16 to 30 minutes. Notably, the major discrepancies between nationalities lie in the importance assigned to the use of SSGs. However, in practice, the formats and objectives for implementing SSGs remain relatively similar.

Jumping Interval Training: An Effective Training Method for Enhancing Anaerobic, Aerobic, and Jumping Performances in Aerobic Gymnastics.

The aim of this study was to compare the effects of jumping interval training (JIT) and running high-intensity interval training (HIIT) on the aerobic, anaerobic and jumping performances of youth female aerobic gymnasts. A randomized controlled study was conducted over an 8-week period, involving 73 youth female athletes (16.2 ± 1.3 years old) of aerobic gymnastics. The study comprised two experimental groups (JIT and HIIT) and a control group. Participants in the experimental groups engaged in two additional training sessions per week alongside their regular training regimen, while the control group followed their usual training routine. Before and after the intervention period, gymnasts were assessed for their performance in the countermovement jump test (CMJ), the specific aerobic gymnastics anaerobic test (SAGAT) and the 20-m multistage fitness test. Significant interactions time × group were found in SAGAT (p < 0.001; = 0.495), CMJ (p < 0.001; = 0.338) and 20-m multistage fitness test (p < 0.001; = 0.500). The time × group analysis post-intervention revealed significantly lower scores in SAGAT for the control group compared to the JIT (p = 0.003) and HIIT (p = 0.034). Additionally, significantly higher scores were observed for the JIT group in the CMJ test compared to the HIIT (p = 0.020) and control (p = 0.028) groups following the intervention. Finally, the 20 m multistage fitness test post-intervention revealed significantly lower scores for the control group compared to JIT (p < 0.001) and HIIT (p < 0.001). Both JIT and HIIT are recommended training strategies to adopt in aerobic gymnastics for significantly improving the aerobic and anaerobic performances of athletes. However, JIT may be particularly relevant to use as it offers additional benefits in improving vertical jumping performances.

Antibodies binding diverse pertactin epitopes protect mice from Bordetella pertussis infection.

Infection by the bacterium Bordetella pertussis continues to cause considerable morbidity and mortality worldwide. Many current acellular pertussis vaccines include the antigen pertactin, which has presumptive adhesive and immunomodulatory activities, but is rapidly lost from clinical isolates after the introduction of these vaccines. To better understand the contributions of pertactin antibodies to protection and pertactin's role in pathogenesis, we isolated and characterized recombinant antibodies binding four distinct epitopes on pertactin. We demonstrate that four of these antibodies bind epitopes that are conserved across all three classical Bordetella strains, and competition assays further showed that antibodies binding these epitopes are also elicited by B. pertussis infection of baboons. Surprisingly, we found that representative antibodies binding each epitope protected mice against experimental B. pertussis infection. A cocktail of antibodies from each epitope group protected mice against a subsequent lethal dose of B. pertussis and greatly reduced lung colonization levels after sublethal challenge. Each antibody reduced B. pertussis lung colonization levels up to 100-fold when administered individually, which was significantly reduced when antibody effector functions were impaired, with no antibody mediating antibody-dependent complement-induced lysis. These data suggest that antibodies binding multiple pertactin epitopes protect primarily by the same bactericidal mechanism, which overshadows contributions from blockade of other pertactin functions. These antibodies expand the available tools to further dissect pertactin's role in infection and understand the impact of antipertactin antibodies on bacterial fitness.

Symmetry-aware deep neural networks for high harmonic spectroscopy in solids.

Neural networks are a prominent tool for identifying and modeling complex patterns, which are otherwise hard to detect and analyze. While machine learning and neural networks have been finding applications across many areas of science and technology, their use in decoding ultrafast dynamics of quantum systems driven by strong laser fields has been limited so far. Here we use standard deep neural networks to analyze simulated noisy spectra of highly nonlinear optical response of a 2-dimensional gapped graphene crystal to intense few-cycle laser pulses. We show that a computationally simple 1-dimensional system provides a useful "nursery school" for our neural network, allowing it to be retrained to treat more complex 2D systems, recovering the parametrized band structure and spectral phases of the incident few-cycle pulse with high accuracy, in spite of significant amplitude noise and phase jitter. Our results offer a route for attosecond high harmonic spectroscopy of quantum dynamics in solids with a simultaneous, all-optical, solid-state based complete characterization of few-cycle pulses, including their nonlinear spectral phase and the carrier envelope phase.

Peritoneal Dialysis Exit-Site Care Protocols in Portugal and Its Association with Catheter-Related Infections.

INTRODUCTION: Exit-site infection (ESi) prevention is a key factor in lowering the risk of peritonitis. This study aimed to evaluate the associations between exit-site (ES) care protocols and the annual incidence rates of ESi and peritonitis in Portugal. METHODS: We performed a national survey using two questionnaires: one about the incidence of catheter-related infections and the other characterizing patients' education and ES care protocols. RESULTS: In 2017 and 2018, 14 Portuguese units followed 764 and 689 patients. ESi incidence rate was 0.41 episodes/year, and the peritonitis incidence rate was 0.37. All units monitor catheter-related infections on a yearly basis, use antibiotic prophylaxis at the time of catheter placement, and treat nasal carriage of S. aureus, although with different approaches. Screening for nasal carriage of S. aureus is performed by 12 units, and daily topical antibiotic cream is recommended by 6 out of 14 of the units. We did not find statistical differences in ESi/peritonitis, comparing these practices. The rate of ESis was lower with nonocclusive dressing immediately after catheter insertion, bathing without ES dressing, with the use of colostomy bags in beach baths and was higher with the use of bath sponge. The peritonitis rate was lower with bathing without ES dressing and if shaving of the external cuff was performed in the presence of chronic ESi. CONCLUSIONS: We found potential proceedings associated with ESi and peritonitis. A regular national audit of peritoneal dialysis units is an important tool for clarifying the best procedures for reduction of catheter-related infections.

COVID-19: Financial Stress and Well-Being in Families.

In all areas of knowledge, research has shown the devastating effects of COVID-19, and the impact on families' financial stress and well-being is one of them. Crises are predictors of families' financial stress as they produce changes in their income and negative feelings, such as fear and demotivation, which affect well-being. This study analyses the financial and social impact of COVID-19 on families, supported by the ABCE-WB model, with data collection being the result of snowball sampling. The results obtained allow the conclusion that the current pandemic crisis has caused financial stress in families, to a greater or lesser degree, and caused feelings of fear and demotivation as consequences of the general lockdown. The empirical evidence also shows that these effects are positively associated with the perception of their level of well-being. The contribution of the study lies in corroborating the model used. Final considerations are presented together with the limitations and suggestions for future research.

Effects of pitch size on soccer players' physiological, physical, technical, and tactical responses during small-sided games: a meta-analytical comparison.

One of the most often-used task constraints in designing small-sided games (SSGs) is the manipulation of pitch size to promote increases or decreases in the relative area per player. Such adjustments cause changes in the acute responses during SSGs. This systematic review with meta-analysis aimed to compare the effects of smaller vs. larger pitch sizes on soccer players' physiological, physical, technical, and tactical responses during SSGs. Comparisons between smaller and larger pitches were not considered based on a specific size, but also between using at least two dimensions in the same comparative study, aiming to understand differences between using smaller and larger (independently of the specific dimensions). The data sources utilized were PubMed, PsycINFO, Scielo, Scopus, SPORTDiscus, and Web of Science. The database search initially yielded 249 titles. From those, 41 articles were eligible for the systematic review and meta-analysis. Results revealed that, compared to smaller pitches, SSGs played on larger pitches induced greater values for heart rate (p < 0.001; ES = 0.50), rate of perceived exertion (p < 0.001; ES = 0.70), total distance (p < 0.001; ES = 1.95), high-speed running (p < 0.001; ES = 1.20), stretch index (p < 0.001; ES = 1.02) and surface area (p < 0.001; ES = 1.54). No significant differences were found between pitch size regarding the numbers of accelerations (p = 0.232; ES = 0.45), decelerations (p = 0.111; ES = 0.85), passes (p = 0.897; ES = 0.02), dribbles (p = 0.823; ES = -0.05), or positional centroid (p = 0.053; ES = 0.56). Larger pitch sizes can be implemented as a meaningful task constraint to increase the internal and external load experienced by soccer players during SSGs, as well as to increase the dispersion of players while acting together. These results were found independent of format and age group.

NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.

The NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the Drosophila ortholog of human RNA helicase Aquarius, is rate-limiting for splicing of a subset of small first introns during oogenesis, including the first intron of gurken Germline depletion of Salsa and splice site mutations within gurken first intron impair both adult female fertility and oocyte dorsal-ventral patterning, due to an abnormal expression of Gurken. Supporting causality, the fertility and dorsal-ventral patterning defects observed after Salsa depletion could be suppressed by the expression of a gurken construct without its first intron. Altogether, our results suggest that one of the key rate-limiting functions of Salsa during oogenesis is to ensure the correct expression and efficient splicing of the first intron of gurken mRNA. Retention of gurken first intron compromises the function of this gene most likely because it undermines the correct structure and function of the transcript 5'UTR.

All-optical valley switch and clock of electronic dephasing.

2D materials with broken inversion symmetry posses an extra degree of freedom, the valley pseudospin, that labels in which of the two energy-degenerate crystal momenta, K or K', the conducting carriers are located. It has been shown that shining circularly-polarized light allows to achieve close to 100% of valley polarization, opening the way to valley-based transistors. Yet, switching of the valley polarization is still a key challenge for the practical implementation of such devices due to the short valley lifetimes. Recent progress in ultrashort laser technology now allows to produce trains of attosecond pulses with controlled phase and polarization between the pulses. Taking advantage of such technology, we introduce a coherent control protocol to turn on, off and switch the valley polarization at faster timescales than electron-hole decoherence and valley depolarization, that is, an ultrafast optical valley switch. We theoretically demonstrate the protocol for hBN and MoS2 monolayers calculated from first principles. Additionally, using two time-delayed linearly-polarized pulses with perpendicular polarization, we show that we can extract the electronic dephasing time T2 from the valley Hall conductivity.

Associations between match participation, maturation, physical fitness, and hormonal levels in elite male soccer player U15: a prospective study with observational cohort.

OBJECTIVES: The aims of this study were to analyze the relationships between minutes of play (MP) and maturity status, fitness, and hormonal levels and to explain how those measures influence the time of play. METHODS: Twenty-six youth soccer players U15 participated in this study over a full-season period. Anthropometric measures, maturity status, growth hormone (GH), insulin-like growth factor and physical levels such as maximal oxygen uptake (VO2max), fatigue index, countermovement jump (CMJ) performance were collected. At the end-season, players were assessed in 6 different tests over four days. RESULTS: VO2max largely correlated with GH (r = 0.57) and CMJ (r = 0.51). Also, GH largely correlated with CMJ (r = 0.55). MP had moderate correlations with VO2max (r = 0.44) and CMJ (r = 0.42). Multiple linear regression with maturation, physical fitness and hormonal levels explained R2 of 0.62 of the MP (F (8, 17) = 3.47, p = 0.015). Although each independent variable alone was not able to determine the playing time, when using the interactions, the model significantly explained the MP. CONCLUSIONS: The combination of maturity status, physical fitness, and hormonal levels seem to play a determinant role in explaining the match participation in youth soccer players.

Does maturity estimation, 2D:4D and training load measures explain physical fitness changes of youth football players?

OBJECTIVES: The purpose of the present study was two-fold: (1) To analyse physical fitness changes of youth football players after a full-season; and (2) to examine whether physical fitness changes are explainable by estimated maturity status, 2digit:4digit ratio (2D:4D) from each hand and training load (TL) measures. METHODS: Twenty-seven youth elite Under-15 football players were daily monitored for training load measures during 38 weeks. At the beginning and at the end of the season, all players were assessed for physical fitness. Also, the maturity status estimation and the length of the second and fourth digits of both hands were collected at the beginning of the season. RESULTS: Significant differences were found for all physical fitness measures after the season. The second and fourth digits of left and right hands had negative moderate correlations with change of direction (COD) changes (r=-.39 to - 0.45 | p = .05 to 0.02). Also, the maturity offset measure had negative moderate correlations with COD changes (r=-.40 | p = .04). From the reported significant correlations, the maturity offset, Left 4D, Right 2D and Right 4D significantly predicted the Mod.505 COD test changes (ß = 0.41, p = .04; ß = -0.41, p = .04; ß = -0.45, p = .02; and ß = -0.44, p = .03, respectively). CONCLUSION: The maturity offset and the 2D:4D measures have the potential to predict COD performance changes over-time in youth football players. Given the lack of associations between the maturity estimation, 2D:4D and training load measures, with the overall physical fitness measures, coaches should rely only at COD changes.