Histological analysis of the intestinal wall of newborn rats submitted to hypoxia and reoxygenation to evaluate the protective effect of N-Acetylcysteine.

PURPOSE: To evaluate the effect of N-Acetylcysteine (NAC) in newborn rats submitted to hypoxia and reoxygenation (H/R) conditions in an experimental model of necrotizing enterocolitis. METHODS: Eight pregnant rats and their 70 cubs were used (5 groups) and exposed to H/R conditions and received NAC at different times. The animals in the H/R groups were placed in a gas chamber (100% CO2) for 10 minutes and then reoxygenated for 10 minutes (100% O2), twice a day for the first three days of life, with a six-hour span between events. On the third day of life, the animals were anesthetized, laparotomized and the intestines were resected. RESULTS: The H/R and NAC groups showed changes in the intestinal wall in relation to the number, height and width of the villi when compared to the control group (p<0.0001), but with better preservation of structures in the NAC group. There were no differences between groups regarding the number (%) of mitoses. CONCLUSION: The administration of NAC decreased the lesions in the intestinal wall of rats submitted to H/R, therefore suggesting that this drug can be used to prevent the development of necrotizing enterocolitis in newborns.

Histological analysis of the intestinal wall of newborn rats submitted to hypoxia and reoxygenation to evaluate the protective effect of N-Acetylcysteine

Abstract Purpose To evaluate the effect of N-Acetylcysteine (NAC) in newborn rats submitted to hypoxia and reoxygenation (H/R) conditions in an experimental model of necrotizing enterocolitis. Methods Eight pregnant rats and their 70 cubs were used (5 groups) and exposed to H/R conditions and received NAC at different times. The animals in the H/R groups were placed in a gas chamber (100% CO2) for 10 minutes and then reoxygenated for 10 minutes (100% O2), twice a day for the first three days of life, with a six-hour span between events. On the third day of life, the animals were anesthetized, laparotomized and the intestines were resected. Results The H/R and NAC groups showed changes in the intestinal wall in relation to the number, height and width of the villi when compared to the control group (p<0.0001), but with better preservation of structures in the NAC group. There were no differences between groups regarding the number (%) of mitoses. Conclusion The administration of NAC decreased the lesions in the intestinal wall of rats submitted to H/R, therefore suggesting that this drug can be used to prevent the development of necrotizing enterocolitis in newborns.

Estudos sobre o impacto da Qualidade do Ar interno na Saúde do Trabalhador / Studies on the Impact of Indoor Air Quality on Workers' Health

Conhecer o impacto da qualidade do ar interior sobre a Saúde do trabalhador, no qual o mesmo é exposto em seu ambiente de trabalho. Foi realizada uma pesquisa retrospectiva documental, no período de fevereiro à maio de 2015, das recomendações para a manutenção e controle da Qualidade do Ar Interno de diferentes instituições, tais como : Organização Mundial de Saúde (OMS), Agência Nacional de Vigilância Sanitária (ANVISA), Associação Brasileira de Refrigeração, Ar Condicionado,Ventilação e Aquecimento (ABRAVA), Associação Brasileira de Normas Técnicas (ABNT), United States Environmental Protection Agency (EPA) e American Society of Heating, Refrigerating and Air-conditioning Engineers (ASHRAE) , além de publicações diversas sobre o tema nos ambientes de trabalho, a partir de fontes terciárias, disponíveis em diferentes bancos de dados, como Pubmed, que reúne diferentes plataformas como Scielo, Lilacs entre outras, publicadas no período de 1995 a 2015. A partir do conhecimento obtido, sugerir uma forma de verificação das condições de qualidade do ar interno no ambiente de trabalho, para promover um ambiente saudável, objetivando minimizar a exposição do trabalhador a poluentes que possam causar danos à sua saúde.