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This study employs novel growth methodologies and surface sensitization with metal nanoparticles to enhance and manipulate gas sensing behavior of two-dimensional (2D)SnS film. Growth of SnS films is optimized by varying substrate temperature and laser pulses during pulsed laser deposition (PLD). Thereafter, palladium (Pd), gold (Au), and silver (Ag) nanoparticles are decorated on as-grown film using gas-phase synthesis techniques. X-ray diffraction (XRD), Raman spectroscopy, and Field-emission scanning electron microscopy (FESEM) elucidate the growth evolution of SnS and the effect of nanoparticle decoration. X-ray photoelectron spectroscopy (XPS) analyses the chemical state and composition. Pristine SnS, Ag, and Au decorated SnS films are sensitive and selective toward NO2 at room temperature (RT). Ag nanoparticle increases the response of pristine SnS from 48 to 138% toward 2 ppm NO2, which indicates electronic and chemical sensitization effect of Ag. Pd decoration on SnS tunes its selectivity toward H2 gas with a response of 55% toward 70 ppm H2 and limit of detection (LOD) < 1 ppm. In situ Kelvin probe force microscopy (KPFM) maps the work function changes, revealing catalytic effect of Ag toward NO2 in Ag-decorated SnS and direct charge transfer between Pd and SnS during H2 exposure in Pd-decorated SnS.
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In the present work, extended X-ray absorption fine-structure (EXAFS) investigations of Co69FexSi21-xB10 (x = 3, 5, 7) glassy ribbons were performed at the Co K-edge. The magnitude of the first peak of the Fourier transforms of the EXAFS signals is found to increase monotonically with increasing Si concentrations indicating the formation of the localized ordered structure at the atomic scale. The Co-Si coordination number (CN) increases at the expense of the CN of Co/Fe. Smaller interatomic distances are observed in the glassy phase compared with that in the crystalline phase which promotes the stability of the glassy phase. Calculations of the thermodynamic parameter (PHSS), cohesive energy (EC) and the atomic radius difference (δ) parameter show that the alloy composition Co69Fe3Si18B10 has a good glass-forming ability (GFA) with the highest CN of Si compared with other compositions. A linear correlation of CN with that of the GFA parameter (PHSS) exists and the CN also plays a crucial role in the GFA of the glassy alloys. This parameter should be considered in developing different GFA criteria.
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Dengue is a serious global health concern especially in tropical and subtropical countries. About 2.5 billion of the world's population is at risk for dengue infection. Early diagnosis is the key to prevent the deterioration of health of the patient to severe illness. Laboratory diagnosis of dengue is essential for providing appropriate supportive treatment to dengue patients with febrile illness, which is difficult to diagnose clinically. Here, we demonstrate surface enhanced Raman scattering (SERS) based diagnosis of dengue virus in clinical blood samples collected from total of 102 subjects. All of the samples were well characterized by conventional NS1 antigen and IgM antibody ELISA kits. The silver nanorods array fabricated by glancing angle deposition technique were employed as SERS substrates. A small amount of patient blood serum (5 µL) was taken for analysis and the report was prepared within a minute. SERS spectra of pure NS1 protein as well as spiked in serum was also recorded separately. Principal component analysis (PCA) was employed as the statistical tool to differentiate dengue positive, dengue negative, and healthy subjects on the basis of their respective SERS spectra. This method provides a sensitive, rapid, and field deployable diagnosis of dengue at the early stage (within 5 days of the onset of symptoms).
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Dengue/diagnóstico , Dengue/sangre , Humanos , Espectrometría Raman , Proteínas no Estructurales Virales/análisisRESUMEN
BACKGROUND: Cardiac resynchronization therapy (CRT) is an established therapy for appropriately selected patients with heart failure. Response to CRT has been heterogeneously defined using both clinical and echocardiographic measures, with poor correlation between the two. METHODS: The study cohort was comprised of 202 CRT-treated patients and CRT response was defined at 6 months post-implant. Echocardiographic response (E+) was defined as a reduction in LVESV ≥ 15%, clinical response as an improvement of ≥ 1 NYHA class (C+), and biomarker response as a ≥ 25% reduction in NT-proBNP(B+). The association of response measures (E+, B+, C+; response score range 0-3) and clinical endpoints at 3 years was assessed in landmarked Cox models. RESULTS: Echo and clinical responders demonstrated greater declines in NT-proBNP than non-responders (median [E+/B+]: -52%, [E+]: -27%, [C+]: -39% and [E-/C-]: -13%; p = 0.01 for trend). Biomarker (HR 0.43 [95% CI: 0.22-0.86], p = 0.02) and clinical (HR 0.40 [0.23-0.70] p = 0.001) response were associated with a significantly reduced risk of the primary endpoint. When integrating each response measure into a composite score, each 1 point increase was associated with a 31% decreased risk for a composite endpoint of mortality, LVAD, transplant and HF hospitalization (HR 0.69 [95% CI: 0.50-0.96], p = 0.03), and a 52% decreased risk of all-cause mortality (HR 0.48 [95% CI: 0.26-0.89], p = 0.02). CONCLUSION: Serial changes in NT-proBNP are associated with clinical outcomes following CRT implant. Integration of biomarker, clinical, and echocardiographic response may discriminate CRT responders versus non-responders in a clinically meaningful way, and with higher accuracy. TRIAL REGISTRATION: The cohort was combined from study NCT01949246 and the study based on local review board approval 2011/550 in Lund, Sweden.
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Dispositivos de Terapia de Resincronización Cardíaca , Terapia de Resincronización Cardíaca , Técnicas de Apoyo para la Decisión , Insuficiencia Cardíaca/terapia , Péptido Natriurético Encefálico/sangre , Fragmentos de Péptidos/sangre , Anciano , Anciano de 80 o más Años , Biomarcadores/sangre , Boston , Terapia de Resincronización Cardíaca/efectos adversos , Toma de Decisiones Clínicas , Ecocardiografía , Femenino , Insuficiencia Cardíaca/sangre , Insuficiencia Cardíaca/diagnóstico por imagen , Insuficiencia Cardíaca/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Selección de Paciente , Valor Predictivo de las Pruebas , Estudios Prospectivos , Recuperación de la Función , Volumen Sistólico , Suecia , Factores de Tiempo , Resultado del Tratamiento , Función Ventricular IzquierdaRESUMEN
Determination of above ground biomass (AGB) of any forest is a longstanding scientific endeavor, which helps to estimate net primary productivity, carbon stock and other biophysical parameters of that forest. With advancement of geospatial technology in last few decades, AGB estimation now can be done using space-borne and airborne remotely sensed data. It is a well-established, time saving and cost effective technique with high precision and is frequently applied by the scientific community. It involves development of allometric equations based on correlations of ground-based forest biomass measurements with vegetation indices derived from remotely sensed data. However, selection of the best-fit and explanatory models of biomass estimation often becomes a difficult proposition with respect to the image data resolution (spatial and spectral) as well as the sensor platform position in space. Using Resourcesat-2 satellite data and Normalized Difference Vegetation Index (NDVI), this pilot scale study compared traditional linear and nonlinear models with an artificial intelligence-based non-parametric technique, i.e. artificial neural network (ANN) for formulation of the best-fit model to determine AGB of forest of the Bundelkhand region of India. The results confirmed the superiority of ANN over other models in terms of several statistical significance and reliability assessment measures. Accordingly, this study proposed the use of ANN instead of traditional models for determination of AGB and other bio-physical parameters of any dry deciduous forest of tropical sub-humid or semi-arid area. In addition, large numbers of sampling sites with different quadrant sizes for trees, shrubs, and herbs as well as application of LiDAR data as predictor variable were recommended for very high precision modelling in ANN for a large scale study.
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Biomasa , Monitoreo del Ambiente/métodos , Redes Neurales de la Computación , Imágenes Satelitales , Carbono/análisis , Bosques , India , Proyectos Piloto , Reproducibilidad de los Resultados , Árboles/crecimiento & desarrolloRESUMEN
Tea has recently received the attention of pharmaceutical and scientific communities due to the plethora of natural therapeutic compounds. As a result, numerous researches have been published in a bid to validate their biological activity. Moreover, major attention has been drawn to antimicrobial activities of tea. Being rich in phenolic compounds, tea has the preventive potential for colon, esophageal, and lung cancers, as well as urinary infections and dental caries, among others. The venture of this review was to illustrate the emerging findings on the antimicrobial properties of different teas and tea extracts, which have been obtained from several in vitro studies investigating the effects of these extracts against different microorganisms. Resistance to antimicrobial agents has become an increasingly important and urgent global problem. The extracts of tea origin as antimicrobial agents with new mechanisms of resistance would serve an alternative way of antimicrobial chemotherapy targeting the inhibition of microbial growth and the spread of antibiotic resistance with potential use in pharmaceutical, cosmetic, and food industries.
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Antiinfecciosos , Camellia sinensis/química , Extractos Vegetales/química , Té/química , Camellia sinensis/crecimiento & desarrollo , Farmacorresistencia Microbiana , Fermentación , Flavonoles/análisis , Manipulación de Alimentos/métodos , Humanos , Extractos Vegetales/farmacología , Hojas de la Planta/química , Especificidad de la EspecieRESUMEN
Shrub-induced soil property spatial heterogeneity is common in arid and semi-arid ecosystems and aids desertified land restoration. However, the effectiveness of this technique may rely on the plant species used and the habitat conditions present. To assess the degree to which planting two native species, Haloxylon salicornicum and Calligonum polygonoides, facilitates degraded land restoration, soil and herbaceous plant community properties were measured 7 years after planting. Soil samples were extracted at two depths (0-5 and 5-20 cm) from three sub-habitats, i.e., under the shrub canopy, from alleys between shrubs and from the open area. Shrub planting increased the quantity of silt + clay content (30-39 %); enhanced water holding capacities (24-30 %); increased the levels of organic carbon (48-69 %), available nitrogen (31-47 %), available phosphorus (32-41 %), and electrical conductivity (21-33 %); and decreased the pH (7-12 %) and bulk density levels (5-6 %) in the surface layer of soils beneath the canopy. Soil property changes were more significant at the surface (0-5 cm) than in the deeper layer (5-20 cm), and were more pronounced under H. salicornicum than under C. polygonoides. Furthermore, the density and biomass levels of herbaceous plants were 1.1 to 1.2 and 1.4 to 1.6 times greater, respectively, in the shrub alleys than in open area. H. salicornicum induced more robust soil amelioration and herbaceous plant facilitative properties than did C. polygonoides. Artificially planting these shrubs may thus be employed to restore degraded areas of arid regions.
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Amaranthaceae/crecimiento & desarrollo , Conservación de los Recursos Naturales/métodos , Magnoliopsida/crecimiento & desarrollo , Clima Desértico , Ecosistema , India , Nitrógeno/análisis , Fósforo/análisis , Suelo/química , Agua/análisisRESUMEN
BACKGROUND: Flexural strength is an important mechanical property that determines the long-term prognosis of interim restorations. Studies are lacking regarding the effect of silanation of the various types of glass fibre impregnation on the flexural strength of resin interim restorations. METHODS: A customized metal die was milled to simulate the prepared abutments of a three-unit fixed dental prosthesis. A total of seventy five samples of interim fixed dental prostheses were prepared using autopolymerizing tooth colour acrylic resin. Unidirectional and woven forms of glass fibres (Stick and Stick Net), which were silane treated and untreated were used to reinforce the resin matrix. Fifteen samples were prepared for each group along with unreinforced group serving as control. The flexural strength was evaluated with universal testing machine. RESULTS: The means and standard deviations of flexural strength for different groups were 13.90 ± 2.96 (control), 61.58 ± 5.26 (unidirectional fibres), 30.89 ± 3.60 (woven fibres), 112.05 ± 5.51 (silane treated unidirectional fibres) and 73.85 ± 4.10 (silane treated woven fibres) respectively. The mean flexural strength of silane treated unidirectional fibres (112.05 MPa) was highest and statistically highly significant (P < 0.0001) compared to all other groups. CONCLUSIONS: Within the limitations of the current study, flexural strength of the reinforced PMMA interim fixed dental prosthesis was significantly higher (P < 0.0001) when compared to the unreinforced PMMA interim fixed dental prosthesis. The use of silane treated unidirectional glass fibres is an effective method of reinforcing interim fixed restorations made of PMMA resins.
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BACKGROUND: Attempts to shorten the overall length of treatment have focused on immediate loading, subsequent to implant placement. Prosthetic rehabilitation immediately after implant placement can be either functional or non-functional in nature. There is paucity of literature on the comparative evaluation of immediate functional and immediate non-functional loading of implants. This in-vivo study was undertaken to comparatively evaluate Immediate Functional Loading and Immediate Non-Functional Loading of monocortical implants with a follow-up period of 18 months. METHODS: 50 partially edentulous cases were selected for the study. The cases were divided into two groups. In first group (Group-1), 25 implants were subjected to immediate functional loading. In second group (Group-2), 25 implants were subjected to immediate non-functional loading. The crestal bone loss, clinical stability and degree of osseointegration of these two groups were comparatively evaluated. RESULTS: The crestal bone loss in both groups was within acceptable limits. The implant stability, which is a reflection of the status of bone-to-implant interface, was comparable in both the groups at different time intervals. Although, the ISQ values in Group-2 were slightly higher than those in Group-1, the results were not statistically significant. Radiodensity indicating degree of osseointegration at different time intervals in both groups was also comparable. CONCLUSION: Both the IFL and INFL protocols can be undertaken satisfactorily in rehabilitation using endosseous implants; however, the main factors for success in IFL and INFL are case selection, meticulous treatment planning and the precision of technique.
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Poland's syndrome is a rare congenital anomaly consisting of a unilateral absence of the pectoralis major, ipsilateral muscle, hand anomaly and occasionally associated other malformations of the chest wall and breast. Many structural and functional abnormalities have been described in association with this syndrome. We report an incidentally diagnosed case in a 27-year-old male patient who presented to us with symbrachydactyly. In addition to this, anterior depression of 2nd, 3rd and 4th ribs and bifid (forked) 5th rib was present on radiological investigations. The body of sternum was short and deformed on the right side with absence of xiphoid process. All middle phalanges were absent on righthand. It is a rare variant of Poland's syndrome.
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Dedos/anomalías , Síndrome de Poland/complicaciones , Sindactilia/complicaciones , Adulto , Dedos/diagnóstico por imagen , Humanos , Masculino , Enfermedades Musculares/complicaciones , Músculos Pectorales/anomalías , Síndrome de Poland/diagnóstico , Radiografía , Costillas/anomalíasRESUMEN
Nanoplastics (NPs) generated from plastic debris weathering pose a significant threat to ecosystems. The ubiquity of plastics driven by their advantageous physical properties, necessitates the development of efficient degradation and removal methods. Polystyrene (PS), a common and hazardous aquatic NP is a long-chain hydrocarbon with alternating phenyl groups. This study investigates the photooxidative degradation of PS NPs under UV light irradiation using synthesized MoO3 nanoflakes, nanobelts, and MoO3/SWCNT nanocomposites. Raman spectroscopy, X-ray diffraction, atomic force microscopy, high-resolution transmission electron microscopy, energy dispersive X-ray, Brunauer-Emmett-Teller, and UV-vis spectroscopy were employed to characterize the photocatalyst. Field emission scanning electron microscopy was used to visualize morphological changes in the spherical PS NPs upon interaction with the photocatalysts. MoO3 nanoflakes acting as a photocatalyst under UV irradiation for 24 h achieved an impressive degradation efficiency exceeding 19%. This treatment significantly reduced the average diameter of PS NPs from 220 to 178 nm. Notably, even higher degradation efficiencies were observed with MoO3 nanobelts and nanocomposites as a complete change in the spherical morphology of PS NPs is observed. Fourier transform infrared spectroscopy elucidated the chemical transformations of PS during degradation. The observed changes in PS NPs structure due to photocatalytic oxidation at different time intervals indicate a promising approach.
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Nanoscale or microscale surface texturing is an effective technique to tailor the tribological properties between two surfaces that are rubbed against each other. In order to achieve the desired frictional properties by a patterned surface, one needs an in-depth understanding of the underlying mechanisms. Here, we demonstrate anisotropic stick-slip friction achieved via a nanotextured surface of tilted titania nanorods (TiNRs). The surface was developed by using the glancing angle deposition (GLAD) technique, and exhibited load-dependent variations in stick-slip friction as well as frictional anisotropy in different sliding directions. For studying the frictional properties of the newly developed surface, lateral force microscopy (LFM) was performed in three different reciprocal orientations (0° rotated, 45° rotated, 90° rotated) using a custom-made colloidal alumina atomic force microscopy (AFM) probe. The frictional behavior was found to vary significantly with the orientation. At 0° rotated position) a prominent "stick-slip" was observed when scanning opposite to the tilt direction, whereas the phenomenon reduced significantly when the nanotextured surface was scanned along the tilt direction or rotated to different angles (45 and 90°) with respect to the sliding direction of the AFM cantilever supporting the probe. The experimental findings were interpreted based on the classical solution for large deflections of tilted elastic rods. Overall, the textured surface, LFM-based frictional measurement, and the quantitative analysis presented here provide a fundamental understanding of how friction can be significantly varied on a surface patterned with tilted TiNRs at a length scale of about 1 µm, which can be comprehensively applied to nanorod patterns of other materials on different substrates.
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Ag2Se is an intriguing material for room-temperature energy harvesting. Herein, we report the fabrication of Ag2Se nanorod arrays by glancing angle deposition technique (GLAD) followed by simple selenization in a two-zone furnace. Ag2Se planar films of different thickness were also prepared. The unique tilted Ag2Se nanorod arrays show excellent zT = 1.14 ± 0.09 and a power factor of 3229.21 ± 149.01 µW/m-K2, respectively, at 300 K. The superior thermoelectric performance of Ag2Se nanorod arrays compared to planar Ag2Se films could be ascribed to the unique nanocolumnar architecture that not only facilitates efficient electron transport but also significantly scatters phonons at the interfaces. Furthermore, the nanoindentation measurements were performed to explore mechanical properties of the as-prepared films. The Ag2Se nanorod arrays showed hardness values of 116.51 ± 4.25 MPa and elastic modulus of 10,966.01 ± 529.61 MPa, which are lowered by 51.8 and 45.6%, compared to Ag2Se films, respectively. The synergetic dependence between the tilt structure and thermoelectric properties accompanied with the simultaneous improvement in mechanical properties opens a new avenue for the practical applications of Ag2Se in next-generation flexible thermoelectric devices.
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Malaria is considered as one the most widespread disease with highest possibility of co-infection at all levels of the disease prognosis. Rapid detection and discrimination of malaria from other co-infections remains a challenge. Hemozoin is a metabolic biproduct of malaraia possessing paramagnetic property due to presence of iron at its centre. Here, we report a label free, rapid and highly sensitive magnetic field based ultra-thin layer chromatography (UTLC) coupled with surface enhanced Raman spectroscopy (SERS) technique for detection and separation of hemozoin from a bacterial mixture. Highly optimized silver nanorods chip fabricated using glancing angle deposition (GLAD) is explored for the UTLC-SERS separation. These chips possessing channel like characteristic and high surface to the volume ratio serve as excellent UTLC plates. The magnetic nature of hemozoin has been exploited for its separation from the mixture of P. aeruginosa (Gram-negative) and S. aureus (Gram-positive) by allocating a 0.6 T magnet over the UTLC flow setup. The solvent front migrated approximately to a distance of 13 mm from the sample point due to the magnetic environment. Spatially resolved SERS data was collected along the mobile phase and separation of mixture was confirmed. Further, staining of hemozoin, P. aeruginosa and S. aureus was done using methylene blue, acridine orange and rhodamine 6 G respectively. The separation was confirmed for the stained analytes. The present developed method provides plate height as low as 18 µm and hemozoin detection limit as <10 parasites/mL. Therefore, we establish a highly specific and sensitive technique capable of separating small amounts of bioanalytes, aiding in the removal of co-infections from the disease at a very early stage of infection.
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Coinfección , Espectrometría Raman , Humanos , Cromatografía en Capa Delgada , Staphylococcus aureus , Campos MagnéticosRESUMEN
In resource limited settings, a cost-effective point-of-care diagnostic testing possessing the characteristics of detecting the minimum viral load of a malady like human immunodeficiency virus (HIV) acquired immune deficiency syndrome (AIDS) is a pressing priority. The present work describes a novel, rapid and field-deployable method using surface enhanced Raman spectroscopy (SERS) for detection and prognosis of HIV positive clinical samples, in seven different viral load ranges varying between 200 and 1 million copies/ml. A relationship between the increasing and decreasing intensity peaks of HIV-1 was also established for quantitation efficacy of the handheld tool. Three different types of SERS substrates: single arm Ag nanorods, double arm Ag nanorods and Au sputtered single arm Ag nanorods were used and the obtained data was compared for the three substrates. It was demonstrated that maximum enhancement was obtained for Au sputtered Ag nanorods. Rigorous coupled wave analysis (RCWA) simulations were performed to study the 'hotspots' in three different SERS substrates. Further, to explore the utility of our platform and to differentiate between the clade specific X4 and R5 tropism, their corresponding SERS spectra were studied using HIV-1 strains belonging to four different HIV-1 subtypes (A, B, C and D) which showed a clear distinction, implying the usefulness of the platform in understanding the disease prognosis. Statistical analysis of the obtained SERS spectra using principal component analysis (PCA) showed good agreement with the experimental results, confirming the ability of SERS platform to quantitate HIV-1 viral load and distinguish HIV-1 strains on the basis of their SERS spectra.
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VIH-1 , Nanotubos , Humanos , Carga Viral , Oro/química , Espectrometría Raman/métodos , Nanotubos/químicaRESUMEN
Background: The giant cell tumour of the tendon sheath (GCTTS) is the second most frequent soft tissue tumour affecting the hand. No consensus exists on the etiology, prognostic factors, or recurrence rate of GCTTS. This article presents a series of 18 cases supplemented by a literature review that examines the epidemiology, presentation, gross and microscopic characteristics, and recurrence rate of GCTTS. Methods: A total of 18 patients with a histo-pathological diagnosis of a GCTTS of the hand or finger were reviewed. The location for the tumour was limited to the wrist and hand. All cases were operated under Wide Awake Local anaesthesia (WALANT), and using a magnifying loupe. Results: A total of 18 patients presented at our institution with a diagnosis of GCTTS from 2016 to 2018. Of the 18 patients, 11 were female and 7 were male. The mean age of included patients was 43.6 years (31-59 years). The most common site for the lesion was the middle finger (4/18), followed by the index finger, wrist, and thumb (3/18 each). The little and ring finger were least commonly affected with one case each. The mean size of the tumour was 2.4 cm (0.5-5 cm). None of the patients reported recurrence of the lesion on an average follow-up of 18.8 months. Conclusion: GCTTS is a benign, slowly growing lesion of the hand that typically does not cause any symptoms and is treated with surgical resection. Meticulous excision of the GCTTS using magnification loupes to ensure appropriate wide excision of the tumour is the treatment of choice to prevent a recurrence. In addition, a radiographic and histopathological examination must be performed on the tumour to rule out other diagnoses. Finally, the function of the hand should be reconstructed to minimize the loss of any functional unit.
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The arid Western Rajasthan, where the Thar Desert of India is immersed, is mostly covered by sand dunes, a common landscape. The region has confronted with fragilities of natural resources, low, erratic and ill-distributed rainfall, and is covered up with many perennial hardy shrubs. Calligonumpolygonoides, the most common perennial shrub, is widely present in some localities of this Thar Desert. In this study, we evaluated the diversity present among 54 wild Calligonum polygonoides plants, sampled from eight different locations within the Thar Desert. Our methods included chemical/nutritional characteristics and random amplified polymorphic DNA (RAPD). Both chemical and molecular methods produced wider range of diversity, however, RAPD detected comparatively more diversity. A total of 163 band positions were produced by ten RAPD primers, of which 147 were found polymorphic with 90.18% polymorphism. RAPD-based Jaccard's similarity coefficients ranged from 0.43-0.89. The analysis of various chemical and mineral constituents revealed that phog is an excellent source of calcium, potassium and phosphorous while relatively poor in zinc. Among minerals, average potassium content was found maximum (2 430mg/100g) with 0.14 CV. Zinc was observed comparably less in quantity while highest variable with CV 0.73. The chemical-based Manhattan dissimilarity coefficient values ranged from 0.01-0.22 with an average of 0.12. The comparison of the clusters obtained based on the chemical and mineral parameters with those of the RAPD data showed that the groups formed in both cases showed different patterns of relationships among the samples. Broader range of diversity might be due to the out breeding behavior of C. polygonoides and indicates the good adaptability of the plants in the region studied. However, low diversity observed in the Bikaner province is alarming and suggests that anthropogenic activities leading to heavy population disturbances can affect the genetic composition of the species in a considerable way.
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Variación Genética/genética , Polygonaceae/química , Polygonaceae/genética , Clima Desértico , India , Repeticiones de Microsatélite , Polygonaceae/clasificación , Técnica del ADN Polimorfo Amplificado AleatorioRESUMEN
Here, we present a portable, selective and cost-effective fiber-optic surface plasmon resonance (SPR) based platform for early detection of Dengue virus. NS1 protein was targeted as the biomarker of dengue. Antibody-antigen specific binding was exploited for NS1 antigen detection. The binding of antibody was assisted by a self-assembled monolayer of alkanethiols on the surface of silver-coated unclad fiber. A wavelength interrogation mode of SPR was utilized to detect NS1 antigen in the dynamic range of 0.2-2.0 µg/ml. The 40 nm thick silver coated optical fiber exhibited resonance wavelength around 500 nm and change in resonance wavelength was monitored for each attachment step on the fiber. The sensitivity at the lowest concentration of NS1 antigen was found to be 54.7 nm/(µg/ml). The limit of detection of the sensor was found to be 0.06 µg/ml, which lies in the physiological range of NS1 protein present in the infected blood, hence the present technique may provide a very early detection advantage. Real blood serum samples were also successfully tested on the set-up, confirming compatibility with the conventional methods. The presented field-deployable platform has wide applications in mass monitoring of dengue, such as during outbreaks and epidemics.
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Técnicas Biosensibles , Dengue , Dengue/diagnóstico , Tecnología de Fibra Óptica , Humanos , Plata , Resonancia por Plasmón de SuperficieRESUMEN
A host association for Syncola crypsimorpha (Meyrick, 1922) is discovered after 100 years, since its original description. In India, two blastobasid species, Syncola crypsimorpha (Meyrick, 1922) and S. pulverea (Meyrick, 1907) (Lepidoptera: Gelechioidea), are predators of cultured lac, Kerria lacca (Kerr, 1782) (Hemiptera: Kerridae). Descriptions, diagnoses, and images and illustrations of the adult stage, including the male and female genitalia, for these two species are provided to facilitate identifications. A lectotype for Syncola crypsimorpha is designated herein.
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Hemípteros , Mariposas Nocturnas , Animales , Femenino , India , MasculinoRESUMEN
BACKGROUND: The ring-less investment system is in use for dental casting, although there was no adequate scientific data to support its use either for conventional fixed dental prosthesis or implant retained fixed dental prosthesis. METHODS: An in-vitro study was undertaken to compare the vertical marginal accuracy of single full coverage metal restorations, between ring-less and metal ring investment techniques, using two different investment materials, for implant supported fixed dental prosthesis. Three groups were made of ten samples each. Group I consisted of metal ring with PCT® FlexVest (phosphate bonded investment material). Group II consisted of metal ring with Bellasun® investment material. Group III and the final group consisted of ring-less investment system and Bellasun® investment material. The wax patterns were prepared on a metal die, cast and finished. The cast restorations (samples) were again seated on the metal die and the accuracy of fit was evaluated by measuring the gap between the finish line on the die and the margins of the sample at four specific sites using a profile projector (Helios-350H, Microtecnica, LTF, Italy) having accuracy of 1µm. RESULT: Mean marginal accuracy for Group-III was found to be the least (58.87 +17.87 µm) followed by Group-II (97.23 + 16.37 µm), and Group-I (109 + 7.55 µm). However, Group I showed the least variability among the readings (SD=7.55). CONCLUSION: Ring-less system of casting can be recommended for use in fabricating implant supported fixed dental restorations.