The final piece of the Triangle of U: Evolution of the tetraploid Brassica carinata genome.

Ethiopian mustard (Brassica carinata) is an ancient crop with remarkable stress resilience and a desirable seed fatty acid profile for biofuel uses. Brassica carinata is one of six Brassica species that share three major genomes from three diploid species (AA, BB, and CC) that spontaneously hybridized in a pairwise manner to form three allotetraploid species (AABB, AACC, and BBCC). Of the genomes of these species, that of B. carinata is the least understood. Here, we report a chromosome scale 1.31-Gbp genome assembly with 156.9-fold sequencing coverage for B. carinata, completing the reference genomes comprising the classic Triangle of U, a classical theory of the evolutionary relationships among these six species. Our assembly provides insights into the hybridization event that led to the current B. carinata genome and the genomic features that gave rise to the superior agronomic traits of B. carinata. Notably, we identified an expansion of transcription factor networks and agronomically important gene families. Completion of the Triangle of U comparative genomics platform has allowed us to examine the dynamics of polyploid evolution and the role of subgenome dominance in the domestication and continuing agronomic improvement of B. carinata and other Brassica species.

Floral hemp (Cannabis sativa L.) responses to nitrogen fertilization under field conditions in the high desert.

While most studies on floral hemp (Cannabis sativa L.) concur that additions of nitrogen (N) increase plant growth, the performance of floral hemp is heavily influenced by environmental conditions, management and cultivar selection. In regions with a short growing season, the availability of soil N may determine plant developmental rates, final inflorescence biomass and cannabinoid concentrations, but no studies have addressed this for field-grown hemp under high-desert conditions. This field study evaluated the effect of no supplemental N and N fertilization at 90 kg ha-1 on three hemp cultivars (Berry Blossom, Red Bordeaux, and Tahoe Cinco) in Northern Nevada. N increased plant height, canopy cover, stem diameter and shoot biomass, but other physiological parameters were dependent on cultivar. For instance, inflorescence biomass and inflorescence-to-shoot ratio in Red Bordeaux was not affected by N fertilization. Similarly, cannabinoid concentrations were affected by timing of harvest and cultivar but not by N treatment. We evaluated the use of a SPAD meter for ease of determining leaf N deficiency, and correlations with leaf chlorophyll content showed that the SPAD meter was a reliable tool in two cultivars but not in Tahoe Cinco. N treatment increased overall CBD yield, which was driven by increases in inflorescence biomass. Tahoe Cinco was the best CBD yielding cultivar, as it maintained a high inflorescence-to-shoot ratio regardless of N treatment. Our study suggests that even though hemp may have a positive response to soil N management, adjustments based on genotype by environment interaction should be aimed at maximizing cannabinoid yield either by increasing biomass and/or CBD concentrations as long as THC levels are within the permissible <0.3% for U.S. industrial hemp cultivation.

Realizing the Potential of Camelina sativa as a Bioenergy Crop for a Changing Global Climate.

Camelina sativa (L.) Crantz. is an annual oilseed crop within the Brassicaceae family. C. sativa has been grown since as early as 4000 BCE. In recent years, C. sativa received increased attention as a climate-resilient oilseed, seed meal, and biofuel (biodiesel and renewable or green diesel) crop. This renewed interest is reflected in the rapid rise in the number of peer-reviewed publications (>2300) containing "camelina" from 1997 to 2021. An overview of the origins of this ancient crop and its genetic diversity and its yield potential under hot and dry growing conditions is provided. The major biotic barriers that limit C. sativa production are summarized, including weed control, insect pests, and fungal, bacterial, and viral pathogens. Ecosystem services provided by C. sativa are also discussed. The profiles of seed oil and fatty acid composition and the many uses of seed meal and oil are discussed, including food, fodder, fuel, industrial, and medical benefits. Lastly, we outline strategies for improving this important and versatile crop to enhance its production globally in the face of a rapidly changing climate using molecular breeding, rhizosphere microbiota, genetic engineering, and genome editing approaches.

Biochar and nitrogen application rates effect on phosphorus removal from a mixed grass sward irrigated with reclaimed wastewater.

This 2-year (2017 and 2018) field study evaluated biochar and nitrogen application rates effect on herbage phosphorus (P) and nitrogen (N) removal from a mixed-grass sward of tall fescue [Schedonorus arundinaceus (Schreb.) Dumort] and Kentucky bluegrass (Poa pratensis L.) irrigated with treated wastewater. Treatments used in this study carried out at the Main Station Field Laboratory, Reno, NV were three biochar application rates (0, 8.9, and 17.8 Mg/ha), and three N rates (0, 80, and 120 kg N/ha) arranged in a 3 × 3 factorial in a randomized complete block design experiment with four replications of each treatment combination. Responses were considered different P < 0.05. There was a linear increased in soil volumetric water content as biochar rate increased from 0 to 17.9 Mg/ha. However, biochar application rate did not affect the quantity of biomass produced, forage tissue P and N concentrations, P and N removal or interact with the other experimental variables of N rate and year to influence the response variables. There was, however, an N rate effect (P < 0.05) on biomass production and it was greater for the 80 and 120 kg N rate (average = 8.3 Mg DM/ha) relative to the 0 kg N/ha rate (6.0 Mg DM/ha). Further, cumulative P removal for the 80 and 120 kg N rate (average = 48.9 kg/ha) was greater than the 0 kg N/ha rate (38.1 kg/ha), and cumulative N removal was in the order 120 kg N/ha (321.1 kg/ha) > 80 kg N/ha (267.4 kg/ha) > 0 kg N/ha (187.8 kg/ha). There was a trend for a biochar × N rate interaction on soil P concentration and it tended to be greater for the combinations 8.9 and 17.8 Mg/ha biochar rates and 80 and 120 kg N/ha rates compared to the unamended control. Even though our study did not reveal a definitive effect of biochar on the major response parameters (biomass, tissue P and N concentrations) evaluated, the trend for a biochar × N rate interaction on soil P concentration offers hope that biochar-amended soils coupled with appropriate N fertilization will be effective in P retention on agricultural landscapes irrigated with treated wastewater.

Characterization of Adult Functional Traits of Local Populations and Cultivars of Sandberg Bluegrass and Bottlebrush Squirreltail Perennial Bunchgrasses.

Plant functional traits offer an understanding of the plant's ability to cope with varying environmental impositions. The objective of this study was to evaluate the above and belowground adult morphological and chemical composition traits of local populations of Sandberg bluegrass (Poa secunda J. Presl) and Bottlebrush squirreltail (Elymus elymoides (Raf.) Swezey) collected in Nevada and their cultivated varieties. A total of six replications (one seedling each) from each population and cultivar of the two native perennial bunchgrasses were used in a randomized complete block design experiment. Each of the six seedlings from each sourced population was transplanted into individual tree pots (28 cm diameter × 61 cm height) containing 20.4 kg of air-dried Orr gravelly sandy loam soil in mid-November, 2015 and remained in the pots for the duration of the study (23 June, 2016). Traits evaluated were, plant height, leaf length, inflorescence length, shoot biomass, forage nutritive value, root morphological traits, and root carbon and nitrogen content. Traits means were considered different at P < 0.05. For Sandberg bluegrass, the cultivar 'Mountain Home' and the population from Panther Valley tended to have greater biomass than the population from Button Point but overall, the average of the two cultivars (10.8 g/plant) did not differ in shoot biomass relative to the local populations (7.6 g/plant). For squirreltail, plant height for the George St. Sonoma and Grass Valley populations (71.3 cm) was greater than the cultivars 'Toe Jam Creek' and 'Vale' (40.5 cm) but cultivars had greater biomass (12.6 g/plant) than the local populations (5.8 g/plant). Total root length and root diameter were not different among the Sanberg bluegrass and squirreltail populations. The results from traits expounded on in this study indicate the closeness of these populations for both species at their adult stage and provide insights for building a unified framework approach among the different agencies and restoration practitioners to aid in plant assemblages for restoration success in the Great Basin and beyond.