RESUMEN
BACKGROUND: This study was aimed at investigating the role and molecular mechanism of Sam68 in cervical cancer lymph node metastasis. MATERIALS AND METHODS: Sam68 expression profile was detected by quantitative polymerase chain reaction, western blotting and immunohistochemical staining. Short hairpin RNA interfering approach was employed to suppress endogenous Sam68 expression in cervical cancer cells to determine its role in metastasis and the possible mechanism. RESULTS: Sam68 expression in cervical cancer was significantly up-regulated at both messenger RNA and protein levels compared with that in normal cervical tissues. The high expression level of Sam68 and its cytoplasmic localization were significantly associated with risk factors including pelvic lymph node metastasis (P < 0.001), and served as independent prognostic factors for predicting shortening of the overall survival time and disease-free survival time in patients with early-stage cervical cancer. Moreover, down-regulation of Sam68 in cervical cancer cells remarkably inhibited cellular motility and invasion. In addition, down-regulation of Sam68 reversed epithelial-mesenchymal transition through inhibiting the Akt/ GSK-3ß/Snail pathway. CONCLUSION: This study demonstrated that Sam68 could induce cervical cancer lymph node metastasis through regulating epithelial-mesenchymal transition, and Sam68 expression profile possessed the potential to serve as predictors of pelvic lymph node metastasis in cervical cancer patients.
Asunto(s)
Proteínas Adaptadoras Transductoras de Señales/biosíntesis , Biomarcadores de Tumor/análisis , Carcinoma de Células Escamosas/patología , Proteínas de Unión al ADN/biosíntesis , Proteínas de Unión al ARN/biosíntesis , Neoplasias del Cuello Uterino/patología , Proteínas Adaptadoras Transductoras de Señales/genética , Adulto , Anciano , Western Blotting , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/metabolismo , Citoplasma/química , Citoplasma/metabolismo , Proteínas de Unión al ADN/genética , Transición Epitelial-Mesenquimal/genética , Femenino , Perfilación de la Expresión Génica , Humanos , Inmunohistoquímica , Estimación de Kaplan-Meier , Metástasis Linfática/genética , Persona de Mediana Edad , Estadificación de Neoplasias , Pronóstico , Proteínas de Unión al ARN/genética , Neoplasias del Cuello Uterino/genética , Neoplasias del Cuello Uterino/metabolismoRESUMEN
PPD-IgG antibody in cerebrospinal fluid (CSF) of 55 patients with tuberculous meningitis (TBM) and 37 patients with non-TBM was determined by ABC-ELISA method, and compared with ELISA. The positive rate of ABC-ELISA and ELISA was 96.4% and 83.6% respectively (P < 0.05). Specificity of two methods was 97.3%. Meanwhile the positive rate in CSF (96.4%) is markedly higher than that in sera (85.5%) (P < 0.05). The results showed that this method can be as a supporting method for diagnosis of TBM.