High-throughput phenotyping reveals expansive genetic and structural underpinnings of immune variation.

By developing a high-density murine immunophenotyping platform compatible with high-throughput genetic screening, we have established profound contributions of genetics and structure to immune variation (http://www.immunophenotype.org). Specifically, high-throughput phenotyping of 530 unique mouse gene knockouts identified 140 monogenic 'hits', of which most had no previous immunologic association. Furthermore, hits were collectively enriched in genes for which humans show poor tolerance to loss of function. The immunophenotyping platform also exposed dense correlation networks linking immune parameters with each other and with specific physiologic traits. Such linkages limit freedom of movement for individual immune parameters, thereby imposing genetically regulated 'immunologic structures', the integrity of which was associated with immunocompetence. Hence, we provide an expanded genetic resource and structural perspective for understanding and monitoring immune variation in health and disease.

IFNγ-Dependent Tissue-Immune Homeostasis Is Co-opted in the Tumor Microenvironment.

Homeostatic programs balance immune protection and self-tolerance. Such mechanisms likely impact autoimmunity and tumor formation, respectively. How homeostasis is maintained and impacts tumor surveillance is unknown. Here, we find that different immune mononuclear phagocytes share a conserved steady-state program during differentiation and entry into healthy tissue. IFNγ is necessary and sufficient to induce this program, revealing a key instructive role. Remarkably, homeostatic and IFNγ-dependent programs enrich across primary human tumors, including melanoma, and stratify survival. Single-cell RNA sequencing (RNA-seq) reveals enrichment of homeostatic modules in monocytes and DCs from human metastatic melanoma. Suppressor-of-cytokine-2 (SOCS2) protein, a conserved program transcript, is expressed by mononuclear phagocytes infiltrating primary melanoma and is induced by IFNγ. SOCS2 limits adaptive anti-tumoral immunity and DC-based priming of T cells in vivo, indicating a critical regulatory role. These findings link immune homeostasis to key determinants of anti-tumoral immunity and escape, revealing co-opting of tissue-specific immune development in the tumor microenvironment.

The GA and ABA signaling is required for hydrogen-mediated seed germination in wax gourd.

BACKGROUND: Hydrogen gas (H2), a novel and beneficial gaseous molecule, plays a significant role in plant growth and development processes. Hydrogen-rich water (HRW) is regarded as a safe and easily available way to study the physiological effects of H2 on plants. Several recent research has shown that HRW attenuates stress-induced seed germination inhibition; however, the underlying modes of HRW on seed germination remain obscure under non-stress condition. RESULTS: In this current study, we investigated the possible roles of gibberellin (GA) and abscisic acid (ABA) in HRW-regulated seed germination in wax gourd (Benincasa hispida) through pharmacological, physiological, and transcriptome approaches. The results showed that HRW application at an optimal dose (50% HRW) significantly promoted seed germination and shortened the average germination time (AGT). Subsequent results suggested that 50% HRW treatment stimulated GA production by regulating GA biosynthesis genes (BhiGA3ox, BhiGA2ox, and BhiKAO), whereas it had no effect on the content of ABA and the expression of its biosynthesis (BhiNCED6) and catabolism genes (BhiCYP707A2) but decreased the expression of ABA receptor gene (BhiPYL). In addition, inhibition of GA production by paclobutrazol (PAC) could block the HRW-mediated germination. Treatment with ABA could hinder HRW-mediated seed germination and the ABA biosynthesis inhibitor sodium tungstate (ST) could recover the function of HRW. Furthermore, RNA-seq analysis revealed that, in the presence of GA or ABA, an abundance of genes involved in GA, ABA, and ethylene signal sensing and transduction might involve in HRW-regulated germination. CONCLUSIONS: This study portrays insights into the mechanism of HRW-mediated seed germination, suggesting that HRW can regulate the balance between GA and ABA to mediate seed germination through ethylene signals in wax gourd.

Effect of N-oleoyl dopamine on myofibroblast trans-differentiation of retinal pigment epithelial cells.

Retinal pigment epithelial (RPE) cells contribute to several clinical conditions resulting in retinal fibrotic scars. Myofibroblast trans-differentiation of RPE cells is a critical step in the process of retinal fibrosis. In this study, we investigated the effects of N-oleoyl dopamine (OLDA), a newer endocannabinoid with a structure distinct from classic endocannabinoids, on TGF-ß2-induced myofibroblast trans-differentiation of porcine RPE cells. Using an in vitro collagen matrix contraction assay, OLDA was found to inhibit TGF-ß2 induced contraction of collagen matrices by porcine RPE cells. This effect was concentration-dependent, with significant inhibition of contraction observed at 3 µM and 10 µM. OLDA did not affect the proliferation of porcine RPE cells. Immunocytochemistry showed that at 3 µM, OLDA decreased incorporation of α-SMA in the stress fibers of TGF-ß2-treated RPE cells. In addition, western blot analysis showed that 3 µM OLDA significantly downregulated TGF-ß2-induced α-SMA protein expression. Taken together these results demonstrate that OLDA inhibits TGF-ß induced myofibroblast trans-differentiation of RPE cells. It has been established that classic endocannabinoid such as anandamide, by activating the CB1 cannabinoid receptor, promote fibrosis in multiple organ systems. In contrast, this study demonstrates that OLDA, an endocannabinoid with a chemical structure distinct from classic endocannabinoids, inhibits myofibroblast trans-differentiation, an important step in fibrosis. Unlike classic endocannabinoids, OLDA has weak affinity for the CB1 receptor. Instead, OLDA acts on non-classic cannabinoid receptors such as GPR119, GPR6, and TRPV1. Therefore, our study indicates that the newer endocannabinoid OLDA and its non-classic cannabinoid receptors could potentially be novel therapeutic targets for treating ocular diseases involving retinal fibrosis and fibrotic pathologies in other organ systems.

Fine mapping and gene silencing pinpoint Capana10g002229 as a strong candidate gene regulating the deciduous character of ripe pepper fruit (Capsicum spp.).

KEY MESSAGE: The pepper S locus, which controls the deciduous character of ripe fruit, was first fine mapped into an interval with a physical length of ~ 38.03 kb on chromosome P10. Capana10g002229, encoding a polygalacturonase, was proposed as a strong candidate gene based on sequence comparison, expression pattern analysis and virus-induced gene silencing (VIGS). The deciduous character of ripe fruit, which is controlled by the dominant S locus, is a domesticated trait with potential value in the pepper processing industry (Capsicum spp.). However, the gene associated with the S locus has not been identified. Here, one major QTL designated S10.1 was detected by using the F2 population (n = 155) derived from BA3 (Capsicum annuum) × YNXML (Capsicum frutescens) and was further verified in an intraspecific backcross population (n = 254) derived from the cross between BB3 (C. annuum) and its wild relative Chiltepin (C. annuum var. glabriusculum) with BB3 as the recurrent parent. Then, a large BC1F2 population derived from the self-pollination of BB3 × (BB3 × Chiltepin) individuals and comprising 4217 individuals was used to screen the recombinants, and the S locus was ultimately delimited into a 38.03-kb region on chromosome P10 harbouring four annotated genes. Capana10g002229, encoding a polygalacturonase (PG), was proposed as the best candidate gene for S based on sequence comparison and expression pattern analyses. Downregulation of Capana10g002229 in fruits through VIGS significantly delayed fruit softening and abscission from the fruit-receptacle junction. Taken together, the results show that Capana10g002229 could be regarded as a strong candidate gene associated with the S locus in pepper. These findings not only lay a foundation for deciphering the molecular mechanisms underlying pepper domestication but also provide a strategy for genetic improvement of the deciduous character of ripe fruit using a marker-assisted selection approach.

Elevated serum anti-Saccharomyces cerevisiae antibody accompanied by gut mycobiota dysbiosis as a biomarker of diagnosis in patients with de novo Parkinson disease.

BACKGROUND AND PURPOSE: Intestinal inflammation and gut microbiota dysbiosis contribute to Parkinson disease (PD) pathogenesis, and growing evidence suggests associations between inflammatory bowel diseases (IBD) and PD. Considered as markers of chronic gastrointestinal inflammation, elevated serum anti-Saccharomyces cerevisiae antibody (ASCA) levels, against certain gut fungal components, are related to IBD, but their effect on PD is yet to be investigated. METHODS: Serum ASCA IgG and IgA levels were measured using an enzyme-linked immunosorbent assay, and the gut mycobiota communities were investigated using ITS2 sequencing and analyzed using the Qiime pipeline. RESULTS: The study included 393 subjects (148 healthy controls [HCs], 140 with PD, and 105 with essential tremor [ET]). Both serum ASCA IgG and IgA levels were significantly higher in the PD group than in the ET and HC groups. Combining serum ASCA levels and the occurrence of constipation could discriminate patients with PD from controls (area under the curve [AUC] = 0.81, 95% confidence interval [CI] = 0.76-0.86) and from patients with ET (AUC = 0.85, 95% CI = 0.79-0.89). Furthermore, the composition of the gut fungal community differed between the PD and HC groups. The relative abundances of Saccharomyces cerevisiae, Aspergillus, Candida solani, Aspergillus flavus, ASV601_Fungi, ASV866_Fungi, and ASV755_Fungi were significantly higher in the PD group, and enriched Malassezia restricta was found in the HC group. CONCLUSIONS: Our study identified elevated serum ASCA levels and enriched gut Saccharomyces cerevisiae in de novo PD.

Boron Removal from Reverse Osmosis Permeate Using an Electrosorption Process: Feasibility, Kinetics, and Mechanism.

Boron is present in the form of boric acid (B(OH)3 or H3BO3) in seawater, geothermal waters, and some industrial wastewaters but is toxic at elevated concentrations to both plants and humans. Effective removal of boron from solutions at circumneutral pH by existing technologies such as reverse osmosis is constrained by high energy consumption and low removal efficiency. In this work, we present an asymmetric, membrane-containing flow-by electrosorption system for boron removal. Upon charging, the catholyte pH rapidly increases to above ∼10.7 as a result of water electrolysis and other Faradaic reactions with resultant deprotonation of boric acid to form B(OH)4- and subsequent removal from solution by electrosorption to the anode. Results also show that the asymmetric flow-by electrosorption system is capable of treating feed streams with high concentrations of boron and RO permeate containing multiple competing ionic species. On the basis of the experimental results obtained, a mathematical model has been developed that adequately describes the kinetics and mechanism of boron removal by the asymmetric electrosorption system. Overall, this study not only provides new insights into boron removal mechanisms by electrosorption but also opens up a new pathway to eliminate amphoteric pollutants from contaminated source waters.

Incorporating social payoff into reinforcement learning promotes cooperation.

Reinforcement learning has been demonstrated to be an effective approach to investigate the dynamic of strategy updating and the learning process of agents in game theory. Most studies have shown that Q-learning failed to resolve the problem of cooperation in well-mixed populations or homogeneous networks. To this aim, we investigate the self-regarding Q-learning's effect on cooperation in spatial prisoner's dilemma games by incorporating the social payoff. Here, we redefine the reward term of self-regarding Q-learning by involving the social payoff; that is, the reward is defined as a monotonic function of the individual payoff and the social payoff represented by its neighbors' payoff. Numerical simulations reveal that such a framework can facilitate cooperation remarkably because the social payoff ensures agents learn to cooperate toward socially optimal outcomes. Moreover, we find that self-regarding Q-learning is an innovative rule that ensures cooperators coexist with defectors even at high temptations to defection. The investigation of the emergence and stability of the sublattice-ordered structure shows that such a mechanism tends to generate a checkerboard pattern to increase agents' payoff. Finally, the effects of Q-learning parameters are also analyzed, and the robustness of this mechanism is verified on different networks.

Chromosome-Level Genome Assembly of a Fragrant Japonica Rice Cultivar 'Changxianggeng 1813' Provides Insights into Genomic Variations between Fragrant and Non-Fragrant Japonica Rice.

East Asia has an abundant resource of fragrant japonica rice that is gaining increasing interest among both consumers and producers. However, genomic resources and in particular complete genome sequences currently available for the breeding of fragrant japonica rice are still scarce. Here, integrating Nanopore long-read sequencing, Illumina short-read sequencing, and Hi-C methods, we presented a high-quality chromosome-level genome assembly (~378.78 Mb) for a new fragrant japonica cultivar 'Changxianggeng 1813', with 31,671 predicated protein-coding genes. Based on the annotated genome sequence, we demonstrated that it was the badh2-E2 type of deletion (a 7-bp deletion in the second exon) that caused fragrance in 'Changxianggeng 1813'. Comparative genomic analyses revealed that multiple gene families involved in the abiotic stress response were expanded in the 'Changxianggeng 1813' genome, which further supported the previous finding that no generalized loss of abiotic stress tolerance associated with the fragrance phenotype. Although the 'Changxianggeng 1813' genome showed high genomic synteny with the genome of the non-fragrant japonica rice cultivar Nipponbare, a total of 289,970 single nucleotide polymorphisms (SNPs), 96,093 small insertion-deletion polymorphisms (InDels), and 8690 large structure variants (SVs, >1000 bp) were identified between them. Together, these genomic resources will be valuable for elucidating the mechanisms underlying economically important traits and have wide-ranging implications for genomics-assisted breeding in fragrant japonica rice.

Cannabidiol Signaling in the Eye and Its Potential as an Ocular Therapeutic Agent.

Cannabidiol (CBD), the major non-intoxicating constituent of Cannabis sativa, has gained recent attention due to its putative therapeutic uses for a wide variety of diseases. CBD was discovered in the 1940s and its structure fully characterized in the 1960s. However, for many years most research efforts related to cannabis derived chemicals have focused on D9-tetrahydrocannabinol (THC). In contrast to THC, the lack of intoxicating psychoactivity associated with CBD highlights the potential of this cannabinoid for clinical drug development. This review details in vitro and in vivo studies of CBD related to the eye, the therapeutic potential of cannabidiol for various ocular conditions, and molecular targets and mechanisms for CBD-induced ocular effects. In addition, challenges of CBD applications for clinical ocular therapeutics and future directions are discussed.

Optimization of photocatalytic degradation conditions and toxicity assessment of norfloxacin under visible light by new lamellar structure magnetic ZnO/g-C3N4.

Degradation of norfloxacin (NFX) by zinc oxide (ZnO)/g-C3N4, a magnetic sheet ZnO with g-C3N4 on its surface was studied. Through a new preparation system method, hydrothermal reaction provides a solid-layered magnetic ZnO material basis, and the simple thermal condensation method was used to transform the urea into g-C3N4 on the magnetic sheet ZnO in a uniform and orderly manner to increase the stability and photocatalytic performance of the material. Compared with previous studies, the pore volume and photocatalytic performance of the material are improved, and became more stable. By studying the degradation effect of basic and photocatalytic materials prepared in different proportions, the kinetic constant of ZGF is 0.01446 (min-1). The response surface methodology (RSM) was used to study the optimization and effect of solution pH (4-12), photocatalyst concentration (0.2-1.8 g/L), and NFX concentration (3-15 mg/L) on the degradation rate of NFX during photocatalytic degradation. The R2 value of the RSM model was 0.9656. The NFX removal rate is higher than 90% when the amount of catalyst is 1.43 g/L, the solution pH is 7.12, and the NFX concentration is less than 8.61 mg/L. After 5 cycles, the degradation rate of magnetic materials decreased to 92.8% of the first time. The capture experiment showed that the photocatalytic machine Toxicities was mainly hole action. The TOC removal rate within 2 h was 30%, a special intermediate toxicity analysis method was adopted according to the characteristics of NFX's inhibitory effect on Escherichia coli community. The toxicity of degraded NFX solution disappeared, and the possibility of non-toxic harm of by-products was verified. LC-Q-TOF method was used to detect and analyze various intermediate products converted from NFX after photocatalytic degradation, and the photocatalytic degradation pathway of NFX was proposed.

Targeting against the activity of the NLRP3 inflammasome is a potential therapy for rat testicular tissue cryopreservation and transplantation.

The objective of the present experiment was to explore the role of NLRP3 inflammasome in the testicular tissue freezing, thawing and grafting; furthermore, the potential effect of a NLRP3 inhibitor on the function of testis transplant was explored. Tissues from male Wistar rats in pre-pubertal age were cryopreserved, thawed and auto-transplanted into the scrotum treated or not treated with the MCC950 (a NLRP3 inhibitor). After grafting, cryopreserved tissue was removed and analysed. Quantitative morphometric, immunohistochemical techniques and Western blotting were used to evaluate the survival of spermatogonia and the activation of the NLRP3 inflammasome after freezing/thawing/grafting. Moreover, serum IL-1ß level was assessed with ELISA kits. The testicular transplants exhibited upregulated expression of the NLRP3 pathway meditors (NLRP3, IL-1ß). In NLRP3 inhibition group, the rate of recovered grafts, the percentage of intact tubules and spermatogonial number were significantly higher than that in cryopreserved graft group. Moreover, serum concentration of IL-1ß in NLRP3 inhibition group was significantly lower than that in cryopreserved graft group. Testicular tissue cryopreservation and transplantation exhibited upregulated expression of NLRP3 pathway and NLRP3 inflammasome blockade improves testicular graft function. These finding suggest that NLRP3 inflammasome is a therapeutic target for testicular tissue cryopreservation and transplantation.

The effects of sodium chloride on proteins aggregation, conformation and gel properties of pork myofibrillar protein Running Head: Relationship aggregation, conformation and gel properties.

The objective of this study was to evaluate relationship with aggregation, secondary structures and gel properties of pork myofibrillar protein with different sodium chloride (1%, 2% and 3%). When the sodium chloride increased from 1 to 3%, the active sulfhydryl, surface hydrophobicity, hardness and cooking yield of myofibrillar protein were increased significantly (p < 0.05), the particle size, total sulfhydryl and Zeta potential were decreased significantly (p < 0.05), these meant the aggregations of pork myofibrillar protein were decreased. The changes of proteins aggregation induced the strongest intensity band of Amide I shifted up from 1660 cm-1 to 1661 cm-1, meanwhile, the ß-sheet structure content was increased significantly (p < 0.05) with the sodium chloride increased. From the above, the lower proteins aggregation and higher ß-sheet structure content could improve the water holding capacity and texture of pork myofibrillar protein gel.

Discovery of endogenous inverse agonists for G protein-coupled receptor 6.

The orphan G protein-coupled receptor 6 (GPR6) is highly expressed in the striatum and has been linked to multiple striatal pathologies. The identification of endogenous ligands and their mechanisms of action at GPR6 will help to elucidate the physiological and pathological roles of the receptor. In the current study, we tested the concentration-dependent effects of a variety of endocannabinoid-like N-acylamides on GPR6 signaling. Here, we demonstrate for the first time that N-arachidonoyl dopamine, N-docosahexaenoyl dopamine, N-oleoyl dopamine and N-palmitoyl dopamine exert inverse agonism at GPR6. This effect was concentration-dependent, with potencies in the micromolar range, and functionally selective for ß-arrestin2 recruitment. Structure-activity relationship studies demonstrate that both the N-acyl side chain and the dopamine head group are important for these ligands to act on GPR6. Our discovery of these N-acyl dopamines as endogenous inverse agonists for GPR6 moves us one step further in understanding the roles GPR6 play in neurodegenerative and neuropsychiatric disorders related to striatal dysfunction.

Involvement of dopamine receptor in the actions of non-psychoactive phytocannabinoids.

Nematode Caenorhabditis elegans (C. elegans) exhibited a vigorous swimming behavior in liquid medium. Addition of dopamine inhibited the swimming behavior, causing paralysis in 65% of wild-type nematodes. Interestingly, phytocannabinoids cannabidiol (CBD) or cannabidivarin (CBDV), caused paralysis in 40% of the animals. Knockout of DOP-3, the dopamine D2-like receptor critical for locomotor behavior, eliminated the paralysis induced by dopamine, CBD, and CBDV. In contrast, both CBD and CBDV caused paralysis in animals lacking CAT-2, an enzyme necessary for dopamine synthesis. Co-administration of dopamine with either CBD or CBDV caused paralysis similar to that of either phytocannabinoid treatment alone. These data support the notion that CBD and CBDV act as functional partial agonists on dopamine D2-like receptors in vivo. The discovery that dopamine receptor is involved in the actions of phytocannabinoids moves a significant step toward our understanding of the mechanisms for medical uses of cannabis in the treatment of neurological and psychiatric disorders.

Micrometer-level 3D measurement techniques in complex scenes based on stripe-structured light and photometric stereo.

Compared with existing depth cameras, such as RGB-D, RealSense and Kinect, stripe-based structured light (SL) has the potential for micrometer-level 3D measurement; this can be attributed to its higher coding capacity. While surface texture, high-reflective region, and occlusion remain some of the main sources leading to degraded reconstruction quality in complex objects, methods that are only based on SL cannot completely solve such problems in complex object reconstruction. In this paper, we developed an advanced fusion strategy for the reconstruction of complex objects in micrometer-level 3D measurement. This includes solving the above-mentioned inherent problems of a stripe-based SL system with the aid of photometric stereo (PS). Firstly, to improve the robustness of decoding and eliminate the effects of noise and occlusion on stripe detection, a novel scene-adaptive decoding algorithm based on a binary tree was proposed. Further, a robust and practical calibration method for area light sources in the PS system, which utilizes the absolute depth information from SL system, was introduced. A piecewise integration algorithm, which is based on a subregion divided by Gray code, was proposed by combining the depth values from SL with the normal information from PS. Remarkably, this method eliminates the effects of surface texture and high-reflective region on the reconstruction quality and improves the resolution to camera-level resolution. In experimental parts, a regular cylinder was reconstructed to demonstrate micrometer-level measurement accuracy and resolution enhancement by the proposed method. Then, improvement of the reconstruction accuracy for objects with surface texture was validated with a regular pyramid that had textures on it and a white paper with characters printed on it. Lastly, a complex object containing multiple phenomena was reconstructed with the newly proposed method to show its effectiveness for micrometer-level 3D measurement in complex objects. Evaluation of our proposed method shows the improvement of the proposed method on the existing methods being used for micrometer-level 3D measurement in complex objects.

Selective Arsenic Removal from Groundwaters Using Redox-Active Polyvinylferrocene-Functionalized Electrodes: Role of Oxygen.

In this work, we investigate selective sorption of arsenic from simulated groundwaters at pH 8 by a redox-active polyvinylferrocene (PVF)-functionalized electrode using a modified double potential step chronoamperometry (DPSC) method. Our results show that effective and sustainable As(III) removal can be achieved at 0 V once the electrode is activated via anodic polarization. During activation, ferrocene (Fc) in PVF is oxidized to the ferrocenium ion (Fc+) with the latter facilitating As(III) sorption and subsequent oxidation as well as As(V) sorption. The high affinity of Fc+ to As and weak attraction to competing anions at 0 V ensure high selectivity of As over Cl-, SO42-, and NO3- at concentrations typical of groundwaters. Following the removal process, efficient regeneration of the electrode is achieved at -1.2 V wherein Fc+ is reduced to Fc thereby facilitating As desorption from the electrode surface. Our results further show that O2 and associated generation of hydrogen peroxide during the regeneration step drive the oxidation of Fc to Fc+, thereby maintaining the constant generation of Fc+ required to achieve As(III) removal in subsequent cycles. Our results show that 91.8 ± 0.6% of As(III) could be selectively removed from simulated groundwater over 10 cycles at an ultralow energy consumption of 0.12 kWh m-3.

Improved aerobic and anaerobic swimming performance after exercise training and detraining in Schizothorax wangchiachii: Implications for fisheries releases.

Swimming performance (aerobic and anaerobic) is often used to predict the ability of fish to adapt and survive. Fish raised in captivity are typically poor swimmers and have lower survival rates than wild conspecifics when released into the natural environment. We investigated the potential for exercise training to enhance the swimming performance of Schizothorax wangchiachii held in captivity. Juvenile fish (mean body mass 1.40 ± 0.13 g, mean body length 4.36 ± 0.24 cm) were trained under five different regimes [3 cm·s-1 control group (C), 10 cm·s-1 for 6 (L6) and 12 h (L12) per day and 20 cm·s-1 for 6 (H6) and 12 h (H12) per day] for 30 days and then detrained for 20 days (i.e. no training). Aerobic (i.e. critical swimming speed, Ucrit), anaerobic swimming performance (i.e. endurance time at 1.2 or 1.5 Ucrit), and morphological parameters were measured at the beginning (T0), after 30 days of exercise training (T30) and after 20 days of detraining (DT20). Aerobic exercise training significantly improved the Ucrit, endurance time at 1.2 and 1.5 Ucrit of juvenile S. wangchiachii (P < .05). After 20 days of detraining, both the aerobic and anaerobic swimming performance of the H6 and H12 groups declined and no longer differed from the control group indicating a failure to maintain improved swimming performance, whereas improved swimming performance was maintained in L6 and L12 groups. No significant difference in swimming performance was found between 6 and 12 hours training at 10 cm·s-1. Thus, exercise at close to 10 cm·s-1 for 6 h per day for 30 days or a longer time periods prior to release appears to be a suitable regime for swimming performance enhancement, potentially increasing survivability of released S. wangchiachii in wild.

Eliminating the Effect of Reflectance Properties on Reconstruction in Stripe Structured Light System.

The acquisition of the geometry of general scenes is related to the interplay of surface geometry, material properties and illumination characteristics. Surface texture and non-Lambertian reflectance properties degrade the reconstruction results by structured light technique. Existing structured light techniques focus on different coding strategy and light sources to improve reconstruction accuracy. The hybrid system consisting of a structured light technique and photometric stereo combines the depth value with normal information to refine the reconstruction results. In this paper, we propose a novel hybrid system consisting of stripe-based structured light and photometric stereo. The effect of surface texture and non-Lambertian reflection on stripe detection is first concluded. Contrary to existing fusion strategy, we propose an improved method for stripe detection to reduce the above factor's effects on accuracy. The reconstruction problem for general scene comes down to using reflectance properties to improve the accuracy of stripe detection. Several objects, including checkerboard, metal-flat plane and free-form objects with complex reflectance properties, were reconstructed to validate our proposed method, which illustrates the effectiveness on improving the reconstruction accuracy of complex objects. The three-step phase-shifting algorithm was implemented and the reconstruction results were given and also compared with ours. In addition, our proposed framework provides a new feasible scheme for solving the ongoing problem of the reconstruction of complex objects with variant reflectance. The problem can be solved by subtracting the non-Lambertian components from the original grey values of stripe to improve the accuracy of stripe detection. In the future, based on stripe structured light technique, more general reflection models can be used to model different types of reflection properties of complex objects.

Modified Double Potential Step Chronoamperometry (DPSC) Method for As(III) Electro-oxidation and Concomitant As(V) Adsorption from Groundwaters.

Constrained by low energy efficiency and ineffectiveness in As(III) removal under circumneutral pH conditions by many exsiting technologies, As(III) removal has become an issue. In this work we present proof of concept of a modified double potential step chronoamperometry (DPSC) method of As(III) oxidation and concomitant As(V) electro-sorption from aqueous solution. Results show that in situ anodic As(III) oxidation, As(V) electro-sorption, and As(V) electro-desorption are affected by aqueous pH with high oxidation and sorption/desorption rates observed at the elevated pH. We particularly show that effective As(III) oxidation and concomitant As(V) adsorption are related to (i) the rapid oxidation of the deprotonated species compared to the protonated species and (ii) stronger electrochemical interaction between the multicharged As(V) species and the electrodes. At 1.2 V and an electric energy consumption of 0.06 kWh m-3, the total As concentration can be reduced from 150 to 15 µg L-1 using an electrochemical cell with electrode area of 10 × 8 cm2 and electro-sorption time of 120 min. On the basis of the experimental results, we have developed a mathematical model to describe the kinetics and mechanism of arsenic removal by the modified DPSC method with this model of use in predicting, and potentially optimizing, process performance under various conditions.