Footprint of the plastisphere on freshwater zooplankton.

Changes in the functional groups of zooplankton were studied in autumn in a temperate floodplain lake (Lake Sakadas, Kopacki Rit Nature Park, Croatia) and in the Drava River (in the Croatian part of the river). Various abiotic parameters as well as available food sources (phytoplankton and microphytes (algae and cyanobacteria) developing on epixylon, epilithon and artificially introduced microplastics called "plastisphere") were also studied. The lake was hydrologically isolated from the main river during the study, while the water level of the Drava River fluctuated, resulting in larger variations in limnological parameters. Due to stable conditions in the lake, zooplankton abundance, biomass, and species richness were higher than in the Drava River. In both environments, zooplankton species feeding on bacteria, detrital suspensions, and small algae were most abundant, with predators and microfilter-feeders being more abundant in the lake. Microphytes were diverse and mostly small and medium-sized in phytoplankton and all substrate types. Stable lake conditions promoted higher abundance of the zooplankton group, which effectively uses larger algae as a food source. The lower abundance of zooplankton feeding on larger algae and predatory species in the river suggests that the epilithon and plastisphere community was a less mature community compared to the lake, and the heterotrophic component with ciliates and/or other small heterotrophs was not well developed. The importance of plastispheres was particularly evident under the turbid hydrologic conditions that prevailed in the river at the end of the study, when phytoplankton biomass decreased and zooplankton abundance steadily increased, suggesting that microphytes colonised on microplastics were an additional food source for higher trophic levels.

Maintenance of High Phytoplankton Diversity in the Danubian Floodplain Lake over the Past Half-Century.

Riverine floodplains are recognized as centers of biodiversity, but due to intense anthropogenic pressures, many active floodplains have disappeared during the last century. This research focuses on the long-term changes in phytoplankton diversity in the floodplain lake situated in the Kopacki Rit (Croatia), one of the largest conserved floodplains in the Middle Danube. The recent dataset from 2003 to 2016 and historical data from the 1970s and 1980s indicate high phytoplankton diversity, summarising 680 taxa for nearly half a century. The variability of species richness is driven by specific in-lake variables, particularly water temperature, water depth, total nitrogen, pH, and transparency, determined by a redundancy analysis of the current data. The high phytoplankton diversity levels are sustained regardless of intense pressures on the lake environment, including exposure to strong anthropogenic pollution in the past and extreme hydrological events, both droughts and floods, which have increasingly affected this part of the Danube in the last decades. The conserved hydrological connection between various biotopes along the river-floodplain gradient seems crucial in maintaining high phytoplankton diversity. Accordingly, conserving natural flooding is mandatory to maintain high biodiversity in complex and dynamic river-floodplain systems.

Distribution of selenium: A case study of the Drava, Danube and associated aquatic biotopes.

This paper presents the results of the research on the overall distribution of selenium (Se) in various aquatic compartments (water, sediment, plankton and macrophytes) at six selected sites of the Croatian part of the Drava and Danube rivers, the connected floodplain lake and the melioration channel system carried out in two sampling periods (flooding in June and the drought period in September). In addition, the physicochemical water properties, plankton composition and biomass were analysed. Our study revealed low mean Se contents in sediments and water, indicating Se deficiency in the studied freshwater systems. The physicochemical environment, including Se distribution, was primarily influenced by hydrology rather than site-specific biogeochemical and morphological characteristics. The flooding period was characterised by higher Se content in water and higher transparency, nitrate and total nitrogen concentrations than drought conditions. At the river sites, sediment Se content was the highest during the flood period, while at all other sites, higher concentrations were found during the drought, reaching the maximum in the lake. Although Se concentrations were below the threshold for aquatic ecotoxicity, they increased in the following order: water (0.021-0.187 µg Se L-1) < sediments (0.005-0.352 mg Se kg-1) < macrophytes (0.010-0.413 mg Se kg-1) < plankton (0.044-0.518 mg Se kg-1) indicating its possible biomagnification at the bottom of the food chain. Species known for high Se accumulation potential dominated the biomass of the main plankton groups and the composition of the macrophyte community, which may provide a more sensitive and accurate steady-state compartment monitor for Se assessment in freshwater biotopes.

Assessment of flood-induced changes of phytoplankton along a river-floodplain system using the morpho-functional approach.

In this research, we aimed to find out how the differences in hydrological connectivity between the main river channel and adjacent floodplain influence the changes in phytoplankton community structure along a river-floodplain system. The research was performed in the River Danube floodplain (Croatian river section) in the period 2008-2009 characterised by different flooding pattern on an annual time scale. By utilising the morpho-functional approach and multivariate analyses, the flood-derived structural changes of phytoplankton were analysed. The lake stability during the isolation phase triggered the specific pattern of morpho-functional groups (MFG) which were characterised by cyanobacterial species achieving very high biomass. Adversely, the high water turbulence in the lake during the frequent and extreme flooding led to evident similarity between lake and river assemblages. Besides different diatom species (groups of small and large centrics and pennates), which are the most abundant representatives in the river phytoplankton, many other groups such as cryptophytes and colonial phytomonads appeared to indicate altered conditions in the floodplain driven by flooding. Having different functional properties, small centric diatom taxa sorted to only one MFG cannot clearly reflect environmental changes that are shown by the species-level pattern. Disadvantages in using the MFG approach highlight that it is still necessary to combine it with taxonomical approach in monitoring of phytoplankton in the river-floodplain ecosystems.

Plastisphere development in relation to the surrounding biotic communities.

To study the early colonization processes, polyethylene terephthalate (PET) microfragments were immersed in Lake Sakadas and the Drava River and sampled weekly together with the surrounding biotic communities - phytoplankton, zooplankton, epixylon in the lake and epilithon in the river. At the end of the study, a rise in water level occurred in the river, which altered the environmental conditions and plankton communities. In studied environments, all of the sampled biotic communities were diverse and abundant. Plastispheres formed in both waters by the seventh day of incubation and developed rapidly, reaching a peak in abundance on the last day of the study. Initial colonization was supported equally by planktonic and periphytic taxa in both environments, but after initial settlement, plastisphere assemblages were affected differently in the river and lake. This study suggests that PET microfragments are a suitable substrate for microphyte settlement and may provide an important pathway for their transport in dynamic freshwater floodplains and river systems.

S-metolachlor promotes oxidative stress in green microalga Parachlorella kessleri - A potential environmental and health risk for higher organisms.

The estimation of the toxic influences of herbicide products on non-target aquatic organisms is essential for evaluation of environmental contamination. We assessed the effects of the herbicide S-metolachlor (S-MET) on unicellular green microalga Parachlorella kessleri during 4-72 in vitro exposure to concentrations in the range 2-200µg/L. The results have shown that S-MET had a significant effect on algae, even in doses 10 and 20 times lower than the EC50 values obtained for P. kessleri (EC50-72h=1090µg/L). It generates reactive oxygen species in algae, decreases their growth and photosynthetic pigment concentration, changes their ultrastructure and alters the cellular antioxidant defence capacities. The levels of protein adducts with the reactive aldehyde 4-hydroxy-2-nonenal (HNE), the end-product of lipid peroxidation, were significantly elevated in S-MET treated cells revealing the insufficient effectiveness of P. kessleri antioxidant mechanisms and persistent lipid peroxidation. Since algae are fundamental aquatic food component, the damaged algal cells, still capable of dividing while having persistently increased content of HNE upon S-MET contamination could represent an important environmental toxic factor that might further affect higher organisms in the food chain.

A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins.

Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment.