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1.
Bioorg Chem ; 108: 104682, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33556696

RESUMEN

Opportunistic parasitic protozoa of genus Acanthamoeba are responsible to cause severe infections in humans such as Acanthamoeba Keratitis or Amoebic Granulomatous Encephalitis. Current treatments are usually toxic and inefficient and there is a need to access new therapeutic agents. The antiamoebic effects of nephthediol (1) and fourteen germacranolide and eudesmanolide sesquiterpene lactones (2-5, 7-12) isolated from the indigenous zoanthid Palythoa aff. clavata collected at the coast of Lanzarote, Canary Islands were studied against Acanthamoeba castellanii Neff, and the clinical strains A. polyphaga and A. griffini. 4-epi-arbusculin A (11) presented the lowest IC50 value (26,47 ± 1,69 µM) against A. castellanii Neff and low cytotoxicity against murine macrophages, followed by isobadgerin (2), which also showed to be active against A. castellanii Neff cysts. The studies on the mode of action of compounds 2 and 11 revealed these sesquiterpene lactones induce mechanisms of PDC on A. castellanii Neff.


Asunto(s)
Acanthamoeba/efectos de los fármacos , Antozoos/química , Antiprotozoarios/farmacología , Lactonas/farmacología , Sesquiterpenos/farmacología , Animales , Antiprotozoarios/química , Antiprotozoarios/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Lactonas/química , Lactonas/aislamiento & purificación , Estructura Molecular , Pruebas de Sensibilidad Parasitaria , Sesquiterpenos/química , Sesquiterpenos/aislamiento & purificación , Relación Estructura-Actividad
2.
Biomed Pharmacother ; 147: 112694, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-35131659

RESUMEN

Naegleria fowleri is the causative agent the primary amoebic meningoencephalitis (PAM), a fatal disease in more than the 90% of the reported cases that affects the central nervous system. The amoeba infects the nasal cavity of mostly children and young adults who report previous aquatic exposure in warm water sources. The rapid progression of the disease and the lack of effective and safety therapeutic options make the search of new anti-amoebic compounds an urgent issue. In this study, twelve sesquiterpene lactones isolated from the zoanthid Palythoa aff. clavata were tested against the trophozoite stage of Naegleria fowleri. Anhydroartemorin (2) and 1(10)Z,4E,14-acetoxy-costunolide (3) showed the best anti-amoeboid activity values with IC50 23.02 ± 1.26 and 28.34 ± 6.27, respectively. In addition, the mechanisms of programmed cell death induction of these two molecules were evaluated with positive results for both compounds. Finally, a structure-activity relationship was analyzed to reveal the dependence of reactivity and lipophilicity on the biological activity. The log P values of the compounds were calculated to postulate them as good candidates to cross the blood-brain barrier, a limiting factor in the development of new anti-Naegleria treatments. Therefore, the mentioned sesquiterpene lactones could be considered as potential PAM therapeutic options in the future.


Asunto(s)
Naegleria fowleri/efectos de los fármacos , Sesquiterpenos/farmacología , Thoracica , Extractos de Tejidos/farmacología , Animales , Apoptosis/efectos de los fármacos , Barrera Hematoencefálica/metabolismo , Línea Celular , Supervivencia Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Sesquiterpenos/química , Relación Estructura-Actividad
3.
Pharmaceuticals (Basel) ; 14(11)2021 Oct 28.
Artículo en Inglés | MEDLINE | ID: mdl-34832876

RESUMEN

Leishmaniasis and Chagas disease are neglected tropical diseases that cause problems in developing countries. The causative agents, Leishmania spp. and Trypanosoma cruzi, produce a clinical picture that can be fatal for the patient, such as Chagas heart disease, visceral leishmaniasis and megacolon, among others. Current treatments for these diseases are not very effective and highly toxic, since they require very prolonged treatments. The development of innovative, effective and safe drugs to fight infections caused by these parasites remains a challenge. For this reason, in recent years, there has been an increase in the search for new therapies. In this study, the antikinetoplastid activity of 13 sesquiterpene lactones obtained from Palythoa aff. clavata was screened against L. amazonensis, L. donovani and T. cruzi. The results revealed that the sesquiterpene lactones anhydroartemorin (2), cis,trans-costunolide-14-acetate (3) and 4-hydroxyarbusculin A (11) were the most selective against the kinetoplastid species studied. These molecules seem to induce the mechanisms involved in an apoptotic-like death or programmed cell death (PCD) in the kinetoplastids, and since they do not cause necrosis, the inflammatory events associated with this type of cell death will not be triggered.

4.
J Nat Prod ; 68(4): 596-9, 2005 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-15844958

RESUMEN

The toxic profile of dinoflagellates varies even among identical species, raising an interesting question about the mechanism of toxin regulation and production. In consequence, it also poses a considerable problem in their control methods. In this paper, we report on the isolation and structural elucidation of several new ester derivatives of okadaic acid (OA) from artificial cultures of the genus Prorocentrum. These new compounds enlarge the range of target molecules that must be considered in the monitoring programs.


Asunto(s)
Dinoflagelados/química , Toxinas Marinas/farmacología , Ácido Ocadaico/análogos & derivados , Ácido Ocadaico/farmacología , Animales , Toxinas Marinas/química , Estructura Molecular , Ácido Ocadaico/química , Intoxicación por Mariscos
5.
J Nat Prod ; 66(9): 1294-6, 2003 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-14510622

RESUMEN

The dinoflagellate Prorocentrum lima produces toxins involved in the red tide phenomenon known as diarrhetic shellfish poisoning (DSP). This paper reports the isolation and spectroscopic structural elucidation of new compounds related to DSP toxins, isolated from a laboratory culture of strain PLV2. Their structures were established from their spectroscopic data.


Asunto(s)
Dinoflagelados/química , Toxinas Marinas/aislamiento & purificación , Ácido Ocadaico/análogos & derivados , Ácido Ocadaico/aislamiento & purificación , Animales , Cromatografía Líquida de Alta Presión , Espectroscopía de Resonancia Magnética , Toxinas Marinas/química , Toxinas Marinas/farmacología , Estructura Molecular , Ácido Ocadaico/química , Ácido Ocadaico/farmacología , Estereoisomerismo , Relación Estructura-Actividad
6.
Chem Rec ; 4(1): 1-9, 2004.
Artículo en Inglés | MEDLINE | ID: mdl-15057864

RESUMEN

Marine toxins have drawn wide interest because their economical impact and disastrous effect upon the shellfish industry and public health in many parts of the world. One of the most interesting group of substances of marine toxins, from structural and pharmacological points of view are polyether compounds, which generally present a great diversity in size and potent biological activities. The subject of this work was about to biosynthesis of okadaic acid skeleton as leader as DSP toxins. Its biosynthesis attracts considerable attention since the carbon skeleton has been shown to be synthesised via an unusual route. In this paper we report on stable isotope incorporation experiments on DSP toxin in artificial cultures of dinoflagellate. The comparison of the degrees of incorporation in these samples measured by different methods led to contradictory results. This implies that further experimental data is needed in order to propose a logical biogenetic scheme.


Asunto(s)
Toxinas Marinas/biosíntesis , Toxinas Marinas/química , Animales , Isótopos de Carbono , Deuterio , Dinoflagelados/metabolismo , Toxinas Marinas/metabolismo , Modelos Moleculares , Estructura Molecular , Ácido Ocadaico/química , Ácido Ocadaico/metabolismo
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