Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p.

OBJECTIVE: Diabetic nephropathy (DN) manifests a critical aspect in the form of renal tubular injury. The current research aimed to determine the function and mechanism of long non-coding ribonucleic acid (LncRNA) differentiation antagonising non-protein coding RNA (DANCR), with a focus on its impact on renal tubular injury. METHODS: Quantitative reverse transcription polymerase chain reaction was employed to analyze the RNA levels of DANCR in the serum of patients with DN or human proximal tubular epithelial cells (human kidney 2 [HK2]). The diagnostic significance of DANCR was assessed using a receiver operating characteristic curve. A DN model was established by inducing HK-2 cells with high glucose (HG). Cell proliferation, apoptosis, and the levels of inflammatory factors, reactive oxygen species (ROS), and malondialdehyde (MDA) were detected using the Cell Counting Kit - 8, flow cytometry, and enzyme-linked immunosorbent assay. The interaction between microRNA (miR)-214-5p and DANCR or Krüppel-like factor 5 (KLF5) was investigated using RNA immunoprecipitation and dual-luciferase reporter assays. RESULTS: Elevated levels of DANCR were observed in the serum of patients with DN and HG-inducted HK-2 cells (P < 0.05). DANCR levels effectively identified patients with DN from patients with type 2 diabetes mellitus. Silencing of DANCR protected against HG-induced tubular injury by restoring cell proliferation, inhibiting apoptosis, and reducing the secretion of inflammatory factors and oxidative stress production (P < 0.05). DANCR functions as a sponge for miR-214-5p, and the mitigation of DANCR silencing on HG-induced renal tubular injury was partially attenuated with reduced miR-214-5p (P < 0.05). Additionally, KLF5 was identified as the target of miR-214-5p. CONCLUSION: DANCR was identified as diagnostic potential for DN and the alleviation of renal tubular injury via the miR-214-5p/KLF5 axis, following DANCR silencing, introduces a novel perspective and approach to mitigating DN.

Ruthenium-Mediated [2 + 2 + 2] Cyclization: A Route to Forge Indane and Isoindoline Core and Its Application in DNA-Encoded Library Technology.

Indane and isoindoline are attractive bicyclic systems in biologically active compounds but are rarely reported in DNA-encoded libraries. In this paper, we reported an efficient and versatile approach for assembling indane and isoindoline scaffolds via a ruthenium-catalyzed [2 + 2 + 2] cyclotrimerization reaction. This method exhibits a broad substrate scope and has been successfully applied to construct a 53K-membered DNA-encoded library (DEL). In order to test its application, we carried out a preliminary selection of this DEL against Aurora A protein and identified a hit compound with 9.3 µM inhibition activity.

Macromolecular Probe Based on a NiII/TbIII Coordination Polymer for Sensitive Recognition of Human Serum Albumin (HSA) and MnO4.

Reported here are the design and fluorescence characters of a NiII/TbIII polymer, [Tb2Ni3(HCAM)6(H2O)12] n (1) (H3CAM = chelidamic acid). Under physiological conditions, the binding of biocompatible water soluble 1 to human serum albumin (HSA) was studied by spectroscopy techniques, which revealed that 1 could inherent the fluorescent light of HSA in a static quencher course and change the HSA second-level structure. The marked enhancement in 1 and its fluorescence intensity provide conclusive evidence that 1 can play the role of a "turn-on" sensor for recognition and detection of HSA in other biological interferents with a K sv value of 7.68 × 104 M-1 and a detection limit of 0.14 µM. Luminescence experiments show that 1 has high selectivity and sensitivity to MnO4 - in other anions. Its quenching efficiency (K sv) is 5.54 × 103 M-1, and the detection limit is 0.29 µM.

Adaptive iterative learning control of a class of nonlinear time-delay systems with unknown backlash-like hysteresis input and control direction.

This paper presents an adaptive iterative learning control scheme for a class of nonlinear systems with unknown time-varying delays and control direction preceded by unknown nonlinear backlash-like hysteresis. Boundary layer function is introduced to construct an auxiliary error variable, which relaxes the identical initial condition assumption of iterative learning control. For the controller design, integral Lyapunov function candidate is used, which avoids the possible singularity problem by introducing hyperbolic tangent funciton. After compensating for uncertainties with time-varying delays by combining appropriate Lyapunov-Krasovskii function with Young's inequality, an adaptive iterative learning control scheme is designed through neural approximation technique and Nussbaum function method. On the basis of the hyperbolic tangent function's characteristics, the system output is proved to converge to a small neighborhood of the desired trajectory by constructing Lyapunov-like composite energy function (CEF) in two cases, while keeping all the closed-loop signals bounded. Finally, a simulation example is presented to verify the effectiveness of the proposed approach.

Determination of 17beta-oestradiol by fluorescence immunoassay with streptavidin-conjugated quantum dots as label.

A sensitive and selective method for the determination of 17beta-oestradiol by fluorescence immunoassay (FIA) was established on the basis of quantum dots (QDs) as label. The complex of biotin-labelled anti-rabbit IgG and strepavidin conjugated by quantum dots (QD-SA) was regarded as a probe in this system and the strepavidin-biotin system as signal amplification system. After optimising the conditions of the immunoreaction, such as the concentration of the reagent and the pH of the buffer solution, the linear range and the limit of detection of 17beta-oestradiol were 0.01-10,000ngml(-1) and 0.00542ngml(-1), respectively. This method was applied to determine oestradiol in water samples, with the percent recoveries in the range of 86-113%.