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1.
Br J Dermatol ; 177(6): 1732-1736, 2017 12.
Artículo en Inglés | MEDLINE | ID: mdl-28369922

RESUMEN

Patients with deficiency of interleukin-36 receptor antagonist (DITRA), due to mutation of IL36RN, exhibit psoriatic phenotypes, typically generalized pustular psoriasis (GPP). We report a paediatric patient with DITRA, whose cutaneous lesions varied from psoriasis vulgaris in infancy to annular pustular psoriasis with acute exacerbation to GPP at 13 years of age. Conventional systemic treatments for GPP, which include oral retinoids, ciclosporin and methotrexate, are controversial in paediatric cases, because of their adverse effects and uncertain long-term consequences. Granulocyte monocyte apheresis, a process associated with few adverse events, promptly controlled the GPP of our paediatric patient, and has potential as a suitable alternative treatment for paediatric patients with DITRA.


Asunto(s)
Citaféresis/métodos , Granulocitos , Interleucinas/genética , Monocitos , Psoriasis/terapia , Adolescente , Humanos , Masculino , Mutación/genética , Psoriasis/genética , Resultado del Tratamiento
4.
Clin Exp Dermatol ; 41(3): 290-3, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26338057

RESUMEN

Mutations in the keratin 10 gene (KRT10) have been shown to underlie several forms of epidermolytic ichthyosis (EI), including generalized, annular and naevoid variants. We investigated an autosomal dominant pedigree with ichthyosis in which there was intrafamilial clinical heterogeneity, with the affected individual family members presenting with features of either erythrokeratoderma progressiva, annular EI, localized or superficial EI, or more generalized EI. Sanger sequencing identified a new heterozygous missense mutation (c.457C>A; p.Leu153Met) in KRT10 in all affected individuals. No additional mutations were identified in the genes for keratin 1 (KRT1) keratin 2 (KRT2), connexin 31 (GJB3) or connexin 30.3 (GJB4) that might account for the clinical heterogeneity seen in this family. Our findings illustrate the intrafamilial variability in phenotype and diverse clinical presentations that can occur in EI resulting from a single mutation in KRT10.


Asunto(s)
Hiperqueratosis Epidermolítica/genética , Queratina-10/genética , Mutación Missense , Adulto , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , Linaje
11.
Br J Dermatol ; 172(5): 1407-11, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25308318

RESUMEN

Autosomal recessive congenital ichthyosis (ARCI) is a genetically heterogeneous disorder for which subtyping through molecular analysis can help determine the eventual phenotype and prognosis. We used whole-exome sequencing to identify a new homozygous splice-site mutation in ST14 (IVS5+1G>A), encoding matriptase, in a 4-year-old girl with ARCI from a consanguineous Kuwaiti family. Clinically, she also had hypotrichosis, which supported a diagnosis of ARCI type 11. Only four previous examples of pathogenic mutations in ST14 have been reported, and our findings expand the genotype-phenotype correlation for this subtype of ARCI. Our patient was the second child born to these parents; the first (deceased) and third children had congenital brain and eye abnormalities, of uncertain aetiology and with no precise diagnosis. Further analysis of our patient's exome dataset revealed heterozygosity for a splice-site mutation in POMT1 (IVS4+1G>T), encoding the protein O-mannosyltransferase, a gene implicated in Walker-Warburg syndrome. DNA sequencing in the third child showed homozygosity for this mutation in POMT1. The first-cousin parents were both heterozygous for the splice-site mutations in ST14 and POMT1. In this family, whole-exome sequencing provided accurate subtyping of a form of ARCI in one child and provide an explanation for an undiagnosed developmental disorder in two other children, findings that improve the prospects for diagnostic accuracy and genetic counselling, and demonstrate the impact of next-generation sequencing technologies on clinical genetics.


Asunto(s)
Trastornos de los Cromosomas/diagnóstico , Ictiosis/diagnóstico , Manosiltransferasas/genética , Mutación/genética , Serina Endopeptidasas/genética , Preescolar , Trastornos de los Cromosomas/genética , Consanguinidad , Exoma , Femenino , Estudio de Asociación del Genoma Completo/métodos , Heterocigoto , Homocigoto , Humanos , Ictiosis/genética , Sitios de Empalme de ARN/genética
12.
Br J Dermatol ; 172(2): 527-31, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25059916

RESUMEN

Only two homozygous nonsense mutations in the epidermal isoform of the dystonin gene, DST-e, have been reported previously in autosomal recessive epidermolysis bullosa simplex (EBS); the affected pedigrees were Kuwaiti and Iranian. This subtype of EBS is therefore considered to be a rare clinicopathological entity. In this study, we identified four seemingly unrelated Kuwaiti families in which a total of seven individuals had predominantly acral trauma-induced blistering since infancy. All affected individuals were homozygous for the mutation p.Gln1124* in DST-e, the same mutation that was identified in the originally reported family from Kuwait. Haplotype analysis in the five pedigrees (including the previous case) revealed a shared block of ~60 kb of genomic DNA across the site of the mutation, consistent with a founder effect. Most heterozygotes had no clinical abnormalities although one subject had mild transient skin fragility during childhood, an observation noted in the previously reported Iranian pedigree, suggesting that the condition may also be semidominant in some pedigrees rather than purely autosomal recessive. Our study reveals propagation of a mutant ancestral allele in DST-e throughout Kuwait, indicating that this subtype of EBS may be more common in Kuwait, and perhaps other Middle Eastern countries, than is currently appreciated.


Asunto(s)
Proteínas Portadoras/genética , Codón sin Sentido/genética , Proteínas del Citoesqueleto/genética , Epidermólisis Ampollosa Simple/genética , Dermatosis del Pie/genética , Dermatosis de la Mano/genética , Proteínas del Tejido Nervioso/genética , Vesícula/genética , Consanguinidad , Distonina , Femenino , Efecto Fundador , Genotipo , Heterocigoto , Homocigoto , Humanos , Kuwait , Masculino , Linaje , Fenotipo , Recurrencia
13.
Br J Dermatol ; 172(4): 1111-5, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25234635

RESUMEN

The physiology of human skin pigmentation is varied and complex, with an extensive melanogenic paracrine network involving mesenchymal and epithelial cells, contributing to the regulation of melanocyte survival and proliferation and melanogenesis. Mutations in several genes, involving predominantly the KIT ligand/c-Kit and Ras/mitogen-activated protein kinase signalling pathways, have been implicated in a spectrum of diseases in which there is hyperpigmentation, hypopigmentation or both. Here, we report on a 12-year-old girl from Taiwan with a 6-year history of diffuse progressive skin hyperpigmentation resulting from a different aetiology: an inborn metabolic disorder of vitamin B12 (cobalamin), designated cblJ. Using whole-exome sequencing we identified a homozygous mutation in ABCD4 (c.423C>G; p.Asn141Lys), which encodes an ATP-binding cassette transporter with a role in the intracellular processing of cobalamin. The patient had biochemical and haematological evidence of cobalamin deficiency but no other clinical abnormalities apart from a slight lightening of her previously black hair. Of note, she had no neurological symptoms or signs. Treatment with oral cobalamin (3 mg daily) led to metabolic correction and some reduction in the skin hyperpigmentation at the 3-month follow-up. This case demonstrates that defects or deficiencies of cobalamin should be remembered in the differential diagnosis of diffuse hyperpigmentary skin disorders.


Asunto(s)
Transportadoras de Casetes de Unión a ATP/genética , Hiperpigmentación/genética , Errores Innatos del Metabolismo/genética , Mutación/genética , Polimorfismo de Nucleótido Simple/genética , Deficiencia de Vitamina B 12/genética , Niño , Femenino , Homocigoto , Humanos , Hiperpigmentación/tratamiento farmacológico , Errores Innatos del Metabolismo/tratamiento farmacológico , Vitamina B 12/uso terapéutico , Deficiencia de Vitamina B 12/tratamiento farmacológico , Complejo Vitamínico B/uso terapéutico
14.
Br J Dermatol ; 172(1): 94-100, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24947307

RESUMEN

BACKGROUND: Subtypes of inherited epidermolysis bullosa (EB) vary significantly in their clinical presentation and prognosis. Establishing an accurate diagnosis is important for genetic counselling and patient management. Current approaches in EB diagnostics involve skin biopsy for immunohistochemistry and transmission electron microscopy, and Sanger sequencing of candidate genes. Although informative in most cases, this approach can be expensive and laborious and may fail to identify pathogenic mutations in ~15% of cases. OBJECTIVES: Next-generation DNA sequencing (NGS) technologies offer a fast and efficient complementary diagnostic strategy, but the value of NGS in EB diagnostics has yet to be explored. The aim of this study was to undertake whole-exome sequencing (WES) in nine cases of EB in which established diagnostic methods failed to make a genetic diagnosis. METHODS: Whole-exome capture was performed using genomic DNA from each case of EB, followed by massively parallel sequencing. Resulting reads were mapped to the human genome reference hg19. Potentially pathogenic mutations were subsequently confirmed by Sanger sequencing. RESULTS: Analysis of WES data disclosed biallelic pathogenic mutations in each case, with all mutations occurring in known EB genes (LAMB3, PLEC, FERMT1 and COL7A1). This study demonstrates that NGS can improve diagnostic sensitivity in EB compared with current laboratory practice. CONCLUSIONS: With appropriate diagnostic platforms and bioinformatics support, WES is likely to increase mutation detection in cases of EB and improve EB diagnostic services, although skin biopsy remains an important diagnostic investigation in current clinical practice.


Asunto(s)
Análisis Mutacional de ADN/métodos , Epidermólisis Ampollosa/diagnóstico , Exoma/genética , Mutación/genética , Adulto , Moléculas de Adhesión Celular/genética , Colágeno Tipo VII/genética , Epidermólisis Ampollosa/genética , Femenino , Humanos , Recién Nacido , Masculino , Proteínas de la Membrana/genética , Persona de Mediana Edad , Proteínas de Neoplasias/genética , Plectina/genética , Kalinina
16.
Clin Exp Dermatol ; 40(5): 529-32, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25683132

RESUMEN

Ichthyosis follicularis, atrichia and photophobia (IFAP) syndrome (OMIM 308205) is a rare X-linked genetic disorder. Mutations in MBTPS2 underlie IFAP syndrome, with 19 different mutations reported to date. Keratosis follicularis spinulosa decalvans (KFSD) is an allelic disorder that results from a single recurrent mutation, p.Asn508Ser. We report a case from the UK of IFAP syndrome resulting from a new mutation, p.Asn508Thr, emphasizing the significant overlap between IFAP and KFSD at both the molecular and clinical levels. An area of alopecia on the scalp of the proband's mother was also noted, suggesting lyonization.


Asunto(s)
Alopecia/genética , Ictiosis/genética , Metaloendopeptidasas/genética , Mutación Missense , Fotofobia/genética , Preescolar , Predisposición Genética a la Enfermedad , Humanos , Masculino , Linaje
17.
Clin Exp Dermatol ; 40(8): 860-4, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26179221

RESUMEN

BACKGROUND: Familial progressive hyper- and hypopigmentation (FPHH) is an autosomal dominant skin condition presenting in childhood with generalized macular dyspigmentation, usually reported in patients of East Asian origin. It overlaps phenotypically with other dyschromatoses, but can now be distinguished by mutations in the KIT ligand gene (KITLG). AIM: We report two unrelated white families with similar phenotypic presentations of FPHH developing in early childhood in several generations. METHODS: Sanger sequencing of the exons and flanking introns of KITLG was performed. RESULTS: This identified a new heterozygous missense mutation in each family (p.Thr34Asn and p.Val37Gly, respectively). Of the six affected individuals examined by us, two had cancer: a 62-year-old man in family 1 had developed two primary melanomas and a pharyngeal carcinoma, and a 42-year-old woman in family 2 had developed thyroid carcinoma. All had unusually sparse lateral eyebrows, a finding not previously reported in this condition. CONCLUSIONS: We summarize the genetic spectrum of the dyschromatoses and discuss a possible increased risk of malignancy in FPHH.


Asunto(s)
Hiperpigmentación/genética , Hipopigmentación/genética , Mutación Missense , Factor de Células Madre/genética , Adulto , Niño , Preescolar , Exones , Femenino , Humanos , Intrones , Masculino , Persona de Mediana Edad , Neoplasias/complicaciones , Linaje
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