A "Dirty" Footprint: Macroinvertebrate diversity in Amazonian Anthropic Soils.

Amazonian rainforests, once thought to be pristine wilderness, are increasingly known to have been widely inhabited, modified, and managed prior to European arrival, by human populations with diverse cultural backgrounds. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by pre-Columbian societies with sedentary habits. Much is known about the chemistry of these soils, yet their zoology has been neglected. Hence, we characterized soil fertility, macroinvertebrate communities, and their activity at nine archeological sites in three Amazonian regions in ADEs and adjacent reference soils under native forest (young and old) and agricultural systems. We found 673 morphospecies and, despite similar richness in ADEs (385 spp.) and reference soils (399 spp.), we identified a tenacious pre-Columbian footprint, with 49% of morphospecies found exclusively in ADEs. Termite and total macroinvertebrate abundance were higher in reference soils, while soil fertility and macroinvertebrate activity were higher in the ADEs, and associated with larger earthworm quantities and biomass. We show that ADE habitats have a unique pool of species, but that modern land use of ADEs decreases their populations, diversity, and contributions to soil functioning. These findings support the idea that humans created and sustained high-fertility ecosystems that persist today, altering biodiversity patterns in Amazonia.

Amazonian earthworm biodiversity is heavily impacted by ancient and recent human disturbance.

Despite the importance of earthworms for soil formation, more is needed to know about how Pre-Columbian modifications to soils and the landscape. Gaining a deeper understanding is essential for comprehending the historical drivers of earthworm communities and the development of effective conservation strategies in the Amazon rainforest. Human disturbance can significantly impact earthworm diversity, especially in rainforest soils, and in the particular case of the Amazonian rainforest, both recent and ancient anthropic practices may be important. Amazonian Dark Earths (ADEs) are fertile soils found throughout the Amazon Basin, created by sedentary habits and intensification patterns of pre-Colombian societies primarily developed in the second part of the Holocene period. We have sampled earthworm communities in three Brazilian Amazonian (ADEs) and adjacent reference soils (REF) under old and young forests and monocultures. To better assess taxonomic richness, we used morphology and the barcode region of the COI gene to identify juveniles and cocoons and delimit Molecular Operational Taxonomic Units (MOTUs). Here we suggest using Integrated Operational Taxonomical units (IOTUs) which combine both morphological and molecular data and provide a more comprehensive assessment of diversity, while MOTUs only rely on molecular data. A total of 970 individuals were collected, resulting in 51 taxonomic units (IOTUs, MOTUs, and morphospecies combined). From this total, 24 taxonomic units were unique to REF soils, 17 to ADEs, and ten were shared between both soils. The highest richness was found in old forest sites for ADEs (12 taxonomic units) and REFs (21 taxonomic units). The beta-diversity calculations reveal a high species turnover between ADEs and REF soils, providing evidence that ADEs and REFs possess distinct soil biota. Furthermore, results suggest that ADE sites, formed by Pre-Columbian human activities, conserve a high number of native species in the landscape and maintain a high abundance, despite their long-term nature.

[Soil mesofauna in differents systems of land use soil in Upper River Solimões, AM, Brazil]. / Mesofauna do solo em diferentes sistemas de uso da terra no Alto Rio Solimões, AM.

The mesofauna has an important function in the soil and it is represented mainly by Acari Oribatida and Collembola. We report the first data on the density and diversity of the soil mesofauna in Benjamin Constant, Amazonas State, Brazil. The following systems were evaluated: primary forest, secondary forest, agroforestry system, cultivated areas and pastures. A total of 101 samples were collected 100 m apart from each other and specimens were collected by using Berlese-Tullgren method. The highest density was registered in secondary forest (29,776 specimens.m-2). Acari Oribatida was the dominant group (7.072 specimens.m-2) in the pasture, suggesting that mites show higher capacity of adaptation to disturbed environments and/or due to the presence of gregarious species. The density of Collembola (5,632 specimens.m-2) was higher in secondary forest. Formicidae was the dominant group (27,824 specimens.m-2) and its highest density occurred in the secondary forest (12,336 specimens.m-2). Seven species and ten morphospecies of Isoptera and three species of Symphyla were identified. The highest density and diversity were found in secondary forest. One supposes that the low density of mesofauna found in all of the studied systems is being influenced by soil structure and composition as well as litter volume. For SUT, the composition of taxonomic groups in the cultivated areas is similar to the one found in primary forest, while the groups found in the agroforestry system are similar to those in the pasture, which may help to decide on land use strategies.

Mesofauna do solo em diferentes sistemas de uso da terra no Alto Rio Solimões, AM / Soil mesofauna in differents systems of land use soil in upper River Solimões, AM, Brazil

The mesofauna has an important function in the soil and it is represented mainly by Acari Oribatida and Collembola. We report the first data on the density and diversity of the soil mesofauna in Benjamin Constant, Amazonas State, Brazil. The following systems were evaluated: primary forest, secondary forest, agroforestry system, cultivated areas and pastures. A total of 101 samples were collected 100 m apart from each other and specimens were collected by using Berlese-Tullgren method. The highest density was registered in secondary forest (29,776 specimens.m-2). Acari Oribatida was the dominant group (7.072 specimens.m-2) in the pasture, suggesting that mites show higher capacity of adaptation to disturbed environments and/or due to the presence of gregarious species. The density of Collembola (5,632 specimens.m-2) was higher in secondary forest. Formicidae was the dominant group (27,824 specimens.m-2) and its highest density occurred in the secondary forest (12,336 specimens.m-2). Seven species and ten morphospecies of Isoptera and three species of Symphyla were identified. The highest density and diversity were found in secondary forest. One supposes that the low density of mesofauna found in all of the studied systems is being influenced by soil structure and composition as well as litter volume. For SUT, the composition of taxonomic groups in the cultivated areas is similar to the one found in primary forest, while the groups found in the agroforestry system are similar to those in the pasture, which may help to decide on land use strategies.