Asunto(s)
Factores de Elongación de Péptidos , Polinucleótidos , ARN de Transferencia , Ribosomas/metabolismo , Acetatos , Acilación , Secuencia de Bases , Isótopos de Carbono , Cromatografía en Gel , Escherichia coli/citología , Escherichia coli/efectos de los fármacos , Escherichia coli/metabolismo , Guanosina Trifosfato/metabolismo , Magnesio/farmacología , Biosíntesis de Péptidos , Factores de Elongación de Péptidos/metabolismo , Factores de Elongación de Péptidos/farmacología , Factores de Iniciación de Péptidos/farmacología , Fenilalanina/metabolismo , Isótopos de Fósforo , Poli U , ARN de Transferencia/metabolismo , Ribosomas/efectos de los fármacos , Tritio , LevadurasRESUMEN
Native Escherichia coli polynucleotide phosphorylase can be retained on blue-dextran--Sepharose. The bound enzyme cannot be displaced by its mononucleotide substrates such as ADP, UDP, CDP, GDP and IDP, but it is easily eluted by its polymeric substrates. Under identical conditions, lactate dehydrogenase, bound on blue-dextran--Sepharose, is not eluted by poly(I) but can be specifically displaced by NADH. On the other hand, the trypsinized polynucleotide phosphorylase, known to be an active enzyme which has lost its polynucleotide site, does not bind to the affinity column. The native polynucleotide phosphorylase can also be tightly bound to poly(U)--agarose and displaced from it only by high salt concentration. The trypsinized enzyme is not bound at all on poly(I)--AGAROSe. Moreover, the native enzyme linked on blue-dextran--Sepharose, remains active indicating a free access of nucleoside diphosphates to the active center. These results taken together show that the dye ligand is not inserted onto the mononucleotide binding site and suggest rather that it binds to the polynucleotide binding region. The implications of this study and the application of blue-dextran--Sepharose affinity chromatography to other proteins having affinity for nucleic acids are discussed.
Asunto(s)
Polirribonucleótido Nucleotidiltransferasa/aislamiento & purificación , Polirribonucleótidos , Cromatografía de Afinidad/métodos , Colorantes , Escherichia coli/enzimología , Cinética , Polirribonucleótido Nucleotidiltransferasa/metabolismo , Ribonucleótidos , SefarosaRESUMEN
Inhibition of soybean lipoxygenase (L-1) and potato 5-lipoxygenase (5-PLO) by the pyrazoline derivatives phenidone and BW755C only occurs after oxidation of these compounds by the peroxidase-like activity of the lipoxygenases. There is a clear relationship between this oxidation and the irreversible inactivation of L-1. The final product of phenidone oxidation by L-1, 4,5-didehydrophenidone, is not responsible of this inactivation, but the species derived from a one-electron oxidation of phenidone plays a key role in L-1 inactivation. In the absence of O2, inactivation of 1 mol of L-1 occurs after the oxidation of 34 mol of phenidone and the covalent binding of 0.8 mol of phenidone-derived metabolite(s) to L-1. In the presence of O2, inactivation of 1 mol of L-1 occurs already after oxidation of 11 mol of phenidone and only involves the covalent binding of 0.4 mol of phenidone-derived metabolite(s) to L-1. A mechanism is proposed for L-1 inactivation by phenidone, which involves the irreversible binding of a phenidone metabolite to the protein and the oxidation of an L-1 amino acid residue (in the presence of O2).
Asunto(s)
4,5-dihidro-1-(3-(trifluorometil)fenil)-1H-pirazol-3-amina/farmacología , Inhibidores de la Lipooxigenasa , Pirazoles/farmacología , Sal Disódica del Ácido 1,2-Dihidroxibenceno-3,5-Disulfónico/farmacología , Araquidonato 5-Lipooxigenasa/sangre , Ácido Ascórbico/farmacología , Catalasa/farmacología , Espectroscopía de Resonancia por Spin del Electrón , Activación Enzimática/efectos de los fármacos , Compuestos Férricos/química , Humanos , Leucocitos/efectos de los fármacos , Leucocitos/enzimología , Lipooxigenasa/química , Oxidación-Reducción , Oxígeno/fisiología , Solanum tuberosum/enzimología , Glycine max/enzimología , Compuestos de Sulfhidrilo/farmacología , Superóxido Dismutasa/farmacologíaRESUMEN
Urine of rats treated with thiophene contains a very major metabolite which represents about 30% of the administered dose. A detailed analysis of its 1H and 13C NMR spectra and a study of its IR and mass spectra clearly showed that it was a 2,5-dihydrothiophene sulfoxide bearing a N-acetyl-cysteinyl group on position 2. Upon heating, it lost water with formation of N-acetyl-S-(2-thienyl)-L-cysteine. A likely mechanism for the formation of this metabolite should involve the S-oxidation of thiophene as a primary step and the addition of glutathione to the very reactive thiophene-S-oxide. These data provide a first evidence for the intermediate formation in vivo of thiophene-S-oxides as reactive metabolites.
Asunto(s)
Acetilcisteína/análogos & derivados , Tiofenos/metabolismo , Tiofenos/orina , Acetilcisteína/química , Acetilcisteína/orina , Animales , Biotransformación , Isótopos de Carbono , Hidrógeno , Espectroscopía de Resonancia Magnética , Masculino , Ratas , Ratas Endogámicas , Tiofenos/química , TritioRESUMEN
A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse cells; (2) its antiviral activity was species specific; (3) its activity was completely neutralized by mouse anti-interferon serum. The synthesis of interferon in this cell-free system requires the presence of spermine.
Asunto(s)
Interferones/biosíntesis , Plantas/metabolismo , Biosíntesis de Proteínas , ARN Mensajero/metabolismo , Reacciones Antígeno-Anticuerpo , Línea Celular , Interferones/inmunología , Interferones/farmacología , Cinética , Simplexvirus/efectos de los fármacos , Especificidad de la Especie , Triticum/metabolismo , Virus de la Estomatitis Vesicular Indiana/efectos de los fármacosRESUMEN
E. coli tryptophanyl-tRNA synthetase can form a complex with Blue-dextran Sepharose, in the presence or in the absence of Mg++. In its absence, the complex is dissociated by either ATP or cognate tRNATrp. However, in the presence of Mg++, only tRNATrp can dissociate the complex whereas ATP has no effect. E. coli total tRNA or tRNAMet, at the same concentration, cannot displace the synthetase from the complex. It is suggested that the Blue-dextran binds to the synthetase through its tRNA binding domain. This hypothesis is supported by previous findings with polynucleotide phosphorylase showing that Blue-dextran Sepharose can be used in affinity chromatography to recognize a polynucleotide binding site of the protein. The selective elution by its cognate tRNA of Trp-tRNA synthetase bound to Blue-dextran Sepharose provides a rapid and efficient purification of the enzyme. Examples of other synthetases and nucleotidyl transferases are also discussed.
Asunto(s)
Aminoacil-ARNt Sintetasas/aislamiento & purificación , Escherichia coli/enzimología , Triptófano-ARNt Ligasa/aislamiento & purificación , Cromatografía de Afinidad/métodos , Magnesio , SefarosaRESUMEN
Mouse interferon (IFN) was produced to high titres after induction of Ehrlich ascites tumour cells with Sendai virus by using an improved procedure. The IFN molecules were labelled during their synthesis by the incorporation of [3H]leucine and [3H]lysine. Electrophoretically homogeneous labelled IFN with a molecular weight of 34000 was obtained after a two-step purification procedure using poly(I)-agarose and octyl-Sepharose column chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The specific radioactivity of this IFN was about 10 ct/min/IFN unit.
Asunto(s)
Interferones/aislamiento & purificación , Virus de la Parainfluenza 1 Humana/inmunología , Animales , Carcinoma de Ehrlich , Células Cultivadas , Cromatografía en Agarosa , Electroforesis en Gel de Poliacrilamida , Interferones/biosíntesis , Ratones , TritioRESUMEN
[1,2-14C]Oct-l-yne was used to investigate metabolic activation of the ethynyl substituent in vitro. Activation of octyne by liver microsomal cytochrome P-450-dependent enzymes gave intermediate(s) that bound covalently to protein, DNA and to haem. The time course and extent of covalent binding of octyne to haem and to protein were similar. However, two different activating mechanisms are probably involved. Whereas covalent binding to protein or to DNA was inhibited by nucleophiles such as N-acetylcysteine, that to haem was little affected. When N-acetylcysteine was included in the reaction mixtures, two major octyne-N-acetylcysteine adducts were isolated and purified by high-pressure liquid chromatography. G.l.c.-mass spectrometry and n.m.r. suggest that these are the cis-trans isomers of S-3-oxo-oct-1-enyl-N-acetylcysteine. Oct-1-yn-3-one reacted non-enzymically with N-acetylcysteine at pH 7.4 and 37 degrees C with a t1/2 of about 6 s also to yield S-3-oxo-oct-l-enyl-N-acetylcysteine. The same product was formed when microsomal fractions were incubated with oct-1-yn-3-ol, N-acetylcysteine and NAD(P)+. Octyn-3-one did not appear to react with haem or protoporphyrin IX. 5. A mechanism for the metabolic activation of oct-1-yne is proposed, consisting in (a) microsomal hydroxylation of the carbon atom alpha to the acetylenic bond and (b) oxidation to yield octyn-3-one as the reactive species.
Asunto(s)
Alquinos/metabolismo , ADN/metabolismo , Hemo/metabolismo , Proteínas/metabolismo , Compuestos de Sulfhidrilo/farmacología , Acetilcisteína/síntesis química , Acetilcisteína/metabolismo , Alquinos/síntesis química , Animales , Cromatografía Líquida de Alta Presión , Técnicas In Vitro , Espectroscopía de Resonancia Magnética , Masculino , Microsomas Hepáticos/metabolismo , Unión Proteica , RatasRESUMEN
Hexanal phenylhydrazone (1; 70:30 E:Z mixture) at micromolar concentration irreversibly inactivates soybean lipoxygenase 1 (L-1) in the presence of dioxygen. L-1 catalyzes the oxidation of 1 into its alpha-azo hydroperoxide 2 [C5H11CH(OOH)N = NC6H5]. 2 is an efficient inactivator of L-1. The aerobic reaction between 1 and L-1 follows a branched pathway leading to the release of 2 into the medium or to L-1 inactivation. The respective parameters corresponding to this inactivation by the (E)-1 and (Z)-1 isomers are Ki = 0.25 and 0.40 microM and kinact = 0.8 and 2.1 min-1. Linoleic acid protection agrees with a mechanism-based inactivation process. The oxidation of a minimum of 13 +/- 3 molar equiv of 1 is required for complete L-1 inactivation, but up to 70 equiv is necessary in the presence of a very large excess of 1. The inactivation is actually the result of two pathways: one is due to a reaction of 2 as soon as it is formed at the active site (20%); the other is due to 2 released into the medium and coming back to the active site (80%). The inactivation is accompanied by the oxidation of 1.8 +/- 0.8 methionine residues of the protein into the corresponding sulfoxide. The inactivated L-1 is electron paramagnetic resonance (EPR) silent with an effective magnetic moment of mu = 5.0 +/- 0.1 Bohr magnetons corresponding to an S = 2 spin state. An inactivation mechanism is proposed on the basis of EPR and magnetic susceptibility data obtained from the anaerobic and aerobic reactions of L-1 with 1 and 2.
Asunto(s)
Hidrazonas/farmacología , Inhibidores de la Lipooxigenasa , Plantas/enzimología , Espectroscopía de Resonancia por Spin del Electrón , Glutatión Peroxidasa/metabolismo , Hidrazonas/síntesis química , Cinética , Lipooxigenasa/aislamiento & purificación , Glycine maxRESUMEN
Human placenta contains, in addition to the ubiquitous p120-GTPase-activating protein (GAP), another isoform of 100 kDa, which is specific to this organ. We have established a method for purifying this placental p100-GAP to near homogeneity. The purified p100-GAP allowed the preparation of polyclonal and monoclonal anti Ras-GAP antibodies. Two monoclonal antibodies were selected for a two-site enzyme immunoassay. This simple and accurate assay in turn facilitated the detection of the GAPs during purification. The purified p100-GAP has a specific activity identical to and catalytic properties similar to those of native p120-GAP. Sequence analysis of p100-GAP revealed almost total identity to the known corresponding sequences predicted by the cDNA. The purified p100-GAP kept its activity for 1 year when stored at -80 degrees C. Our immunometric assay showed GAP to be present in human placental extracts at the exceptional abundance of about 0.1% of the total protein content. Quantitative assays showed p100-GAP to be up to 10 times more abundant than p120-GAP. Use of our antibodies allowed the specific localization of placental GAPs to cytotrophoblasts and in the syncytiotrophoblast barrier. Hence p100-GAP is shown to be found only in trophoblasts. The large quantity of p100-GAP in trophoblasts suggests that it may play a regulatory role in the proliferation or the differentiation of this cell type.
Asunto(s)
Placenta/química , Proteínas/aislamiento & purificación , Secuencia de Aminoácidos , Anticuerpos Monoclonales , Western Blotting , Electroforesis en Gel de Poliacrilamida , Femenino , Proteínas Activadoras de GTPasa , Humanos , Técnicas para Inmunoenzimas , Datos de Secuencia Molecular , Peso Molecular , Fragmentos de Péptidos/química , Proteínas/química , Proteínas/metabolismo , Análisis de Secuencia , Distribución Tisular , Trofoblastos/química , Proteínas Activadoras de ras GTPasaRESUMEN
Human leukocyte interferon can be separated into two classes of subspecies by polynucleotide-agarose affinity chromatography; 30-40% of the molecular species have the polynucleotide-binding property and 60-70% lack affinity for the polynucleotide ligand. When analyzed on sodium dodecyl sulfate/polyacrylamide gel electrophoresis, the former class of interferon has a slower mobility corresponding to the migration of a polypeptide of 21,000 daltons, while the latter class has a faster mobility corresponding to a polypeptide of 13,500-15,000 daltons. By analogy to the behavior of other interferons and a class of nucleotidyl transferases on the polynucleotide-agarose chromatography, we suggest that the human leukocyte interferon having the polynucleotide-binding site is in a possibly "native" conformation and the loss of affinity for polynucleotide results from a degradative alteration of the native molecules. Moreover, the alteration of interferon is accompanied by an increase in heterospecific activity on bovine cells. It is suggested that the polypeptide domain responsible for species specificity may be closely related to the polynucleotide binding area. The modified interferon molecule, however, still conserves its antiviral activity. The simplicity and the high capacity of polynucleotide-agarose chromatography make this a powerful technique for the purification of interferon. The easy separation of these two classes of human leukocyte interferon makes the purification procedures more rational and will facilitate the preparation of both subspecies to a high degree of molecular homogeneity.