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In recent years, Climate-Smart Forestry (CSF) has emerged as an innovative approach to sustainable forest management, aiming to enhance forest resilience and to balance the provision of ecosystem services facing climate-related threats. This study introduces for the first time a new composite climate-smart index (ICSF) to assess CSF. The methodological approach comprises the following steps: (i) the selection and evaluation of CSF indicators; (ii) the weighting of these indicators; and (iii) the assessment of CSF for Mediterranean forests in two distinct periods, specifically 2005 and 2015. Eight indicators were selected from a systematic literature review. The Analytic Hierarchy Process was applied to translate the preferences obtained through an online questionnaire from a network of CSF-expert stakeholders into weights, at both indicators and criteria levels (i.e., adaptation, mitigation, and the social dimension). Results reveals that indicators "tree species composition", "forest damage", and "regeneration" are of crucial importance for CSF assessment. The comparison of the CSF value between the years 2005 and 2015, shows a slight increase in CSF ratings. The ICSF serves as a comprehensive index of CSF covering all aspects of that concept, i.e. adaptation, mitigation, and the social dimension (including production). The national-scale analysis provides an overview of the dynamics that involve forest management of Mediterranean forests against climate change. The study offers a practicable method for CSF evaluation with its allover set of indicators, representing a suitable tool for supporting forest managers to mitigate the negative impacts of climate change.
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Despite growing interest in predicting plant phenological shifts, advanced spring phenology by global climate change remains debated. Evidence documenting either small or large advancement of spring phenology to rising temperature over the spatio-temporal scales implies a potential existence of a thermal threshold in the responses of forests to global warming. We collected a unique data set of xylem cell-wall-thickening onset dates in 20 coniferous species covering a broad mean annual temperature (MAT) gradient (-3.05 to 22.9°C) across the Northern Hemisphere (latitudes 23°-66° N). Along the MAT gradient, we identified a threshold temperature (using segmented regression) of 4.9 ± 1.1°C, above which the response of xylem phenology to rising temperatures significantly decline. This threshold separates the Northern Hemisphere conifers into cold and warm thermal niches, with MAT and spring forcing being the primary drivers for the onset dates (estimated by linear and Bayesian mixed-effect models), respectively. The identified thermal threshold should be integrated into the Earth-System-Models for a better understanding of spring phenology in response to global warming and an improved prediction of global climate-carbon feedbacks.
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Tracheophyta , Teorema de Bayes , Bosques , Frío , Temperatura , Cambio Climático , Estaciones del AñoRESUMEN
Wood formation consumes around 15% of the anthropogenic CO2 emissions per year and plays a critical role in long-term sequestration of carbon on Earth. However, the exogenous factors driving wood formation onset and the underlying cellular mechanisms are still poorly understood and quantified, and this hampers an effective assessment of terrestrial forest productivity and carbon budget under global warming. Here, we used an extensive collection of unique datasets of weekly xylem tissue formation (wood formation) from 21 coniferous species across the Northern Hemisphere (latitudes 23 to 67°N) to present a quantitative demonstration that the onset of wood formation in Northern Hemisphere conifers is primarily driven by photoperiod and mean annual temperature (MAT), and only secondarily by spring forcing, winter chilling, and moisture availability. Photoperiod interacts with MAT and plays the dominant role in regulating the onset of secondary meristem growth, contrary to its as-yet-unquantified role in affecting the springtime phenology of primary meristems. The unique relationships between exogenous factors and wood formation could help to predict how forest ecosystems respond and adapt to climate warming and could provide a better understanding of the feedback occurring between vegetation and climate that is mediated by phenology. Our study quantifies the role of major environmental drivers for incorporation into state-of-the-art Earth system models (ESMs), thereby providing an improved assessment of long-term and high-resolution observations of biogeochemical cycles across terrestrial biomes.
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Tracheophyta/crecimiento & desarrollo , Madera/crecimiento & desarrollo , Xilema/crecimiento & desarrollo , Clima , Cambio Climático , Ecosistema , Bosques , Calentamiento Global , Modelos Biológicos , Fotoperiodo , Estaciones del Año , Temperatura , Tracheophyta/genética , Árboles/crecimiento & desarrolloRESUMEN
INTRODUCTION: Silver fir (Abies alba Mill.) is one of the most valuable conifer wood species in Europe. Among the main opportunistic pathogens that cause root and butt rot on silver fir are Armillaria ostoyae and Heterobasidion abietinum. Due to the different enzymatic pools of these wood-decay fungi, different strategies in metabolizing the phenols were available. OBJECTIVE: This work explores the changes in phenolic compounds during silver fir wood degradation. METHODOLOGY: Phenols were analyzed before and after fungus inoculation in silver fir macerated wood after 2, 4 and 6 months. All samples were analyzed using high-performance liquid chromatography coupled to a hybrid quadrupole-orbitrap mass spectrometer. RESULTS: Thirteen compounds, including simple phenols, alkylphenyl alcohols, hydroxybenzoketones, hydroxycinnamaldehydes, hydroxybenzaldehydes, hydroxyphenylacetic acids, hydroxycinnamic acids, hydroxybenzoic acids and hydroxycoumarins, were detected. Pyrocatechol, coniferyl alcohol, acetovanillone, vanillin, benzoic acid, 4-hydroxybenzoic acid and vanillic acid contents decreased during the degradation process. Methyl vanillate, ferulic acid and p-coumaric were initially produced and then degraded. Scopoletin was accumulated. Pyrocatechol, acetovanillone and methyl vanillate were found for the first time in both degrading and non-degrading wood of silver fir. CONCLUSIONS: Despite differences in the enzymatic pool, both fungi caused a significant decrease in the amounts of phenolic compounds with the accumulation of the only scopoletin. Principal component analysis revealed an initial differentiation between the degradation activity of the two fungal species during degradation, but similar phenolic contents at the end of wood degradation.
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Fenoles , Madera , Cromatografía Líquida de Alta Presión , Hongos/metabolismo , Hidroxibenzoatos/análisis , Fenoles/análisis , Madera/químicaRESUMEN
Mesophyll conductance (gm) determines the diffusion of CO2 from the substomatal cavities to the site of carboxylation in the chloroplasts and represents a critical component of the diffusive limitation of photosynthesis. In this study, we evaluated the average effect sizes of different environmental constraints on gm in Populus spp., a forest tree model. We collected raw data of 815 A-Ci response curves from 26 datasets to estimate gm, using a single curve-fitting method to alleviate method-related bias. We performed a meta-analysis to assess the effects of different abiotic stresses on gm. We found a significant increase in gm from the bottom to the top of the canopy that was concomitant with the increase of maximum rate of carboxylation and light-saturated photosynthetic rate (Amax). gm was positively associated with increases in soil moisture and nutrient availability, but was insensitive to increasing soil copper concentration and did not vary with atmospheric CO2 concentration. Our results showed that gm was strongly related to Amax and to a lesser extent to stomatal conductance (gs). Moreover, a negative exponential relationship was obtained between gm and specific leaf area, which may be used to scale-up gm within the canopy.
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Populus , Dióxido de Carbono , Células del Mesófilo , Fotosíntesis , Hojas de la Planta , Estomas de Plantas , Estrés FisiológicoRESUMEN
Conductance of CO2 across the mesophyll (Gm) frequently constrains photosynthesis (PN) but cannot be measured directly. We examined Gm of cherry (Prunus avium L.) subjected to severe drought using the variable J method and carbon-isotopic composition (δ13C) of sugars from the centre of the leaf, the leaf petiole sap, and sap from the largest branch. Depending upon the location of the plant from which sugars are sampled, Gm may be estimated over scales ranging from a portion of the leaf to a canopy of leaves. Both the variable J and δ13C of sugars methods showed a reduction in Gm as soil water availability declined. The δ13C of sugars further from the source of their synthesis within the leaf did not correspond as closely to the diffusive and C-isotopic discrimination conditions reflected in the instantaneous measurement of gas exchange and chlorophyll-fluorescence utilised by the variable J approach. Post-photosynthetic fractionation processes and/or the release of sugars from stored carbohydrates (previously fixed under different environmental and C-isotopic discrimination conditions) may reduce the efficacy of the δ13C of sugars from leaf petiole and branch sap in estimating Gm in a short-term study. Consideration should be given to the spatial and temporal scales at which Gm is under observation in any experimental analysis.
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Isótopos de Carbono/metabolismo , Sequías , Células del Mesófilo/metabolismo , Hojas de la Planta/metabolismo , Prunus avium/metabolismo , Azúcares/metabolismo , Dióxido de Carbono/química , Dióxido de Carbono/metabolismo , Clorofila/metabolismo , Modelos Biológicos , Fotosíntesis/fisiología , Suelo , Azúcares/química , AguaRESUMEN
The allocation and stoichiometry of plant nutrients in leaves reflect fundamental ecosystem processes, biotic interactions, and environmental drivers such as water availability. Climate change will lead to increases in drought severity and frequency, but how canopy nutrients will respond to drought, and how these responses may vary with community composition along aridity gradients is poorly understood. We experimentally addressed this issue by reducing precipitation amounts by 66% during two consecutive growing seasons at three sites located along a natural aridity gradient. This allowed us to assess drought effects on canopy nitrogen (N) and phosphorus (P) concentrations in arid and semiarid grasslands of northern China. Along the aridity gradient, canopy nutrient concentrations were positively related to aridity, with this pattern was driven primarily by species turnover (i.e., an increase in the relative biomass of N- and P-rich species with increasing aridity). In contrast, drought imposed experimentally increased N but decreased P concentrations in plant canopies. These changes were driven by the combined effects of species turnover and intraspecific variation in leaf nutrient concentrations. In addition, the sensitivity of canopy N and P concentrations to drought varied across the three sites. Canopy nutrient concentrations were less affected by drought at drier than wetter sites, because of the opposing effects of species turnover and intraspecific variation, as well as greater drought tolerance for nutrient-rich species. These contrasting effects of long-term aridity vs. short-term drought on canopy nutrient concentrations, as well as differing sensitivities among sites in the same grassland biome, highlight the challenge of predicting ecosystem responses to future climate change.
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Sequías , Ecosistema , China , Cambio Climático , NutrientesRESUMEN
The sediments dredged from a waterway and decontaminated through a phytoremediation process have been used as substrates alternatively to the traditional forest nursery substrate for pot productions of holm oak (Quercus ilex L.) planting stocks. The substrates, made by mixing decontaminated sediments to agricultural soil at different degrees, were tested in order to evaluate their suitability as growth substrates. The experiment was carried out at the nursery of the Department of Agricultural, Food and Forestry Systems of the University of Florence (Italy). The experimental design consisted of four randomized blocks with six pots as replicates for each of the following treatments: 100% sediments, 66% sediments, 33% sediments, 100% agronomic soil and 100% traditional peat based substrate. In each pot, one holm oak acorn was seeded. Germination and both physiological and morphological traits of the seedlings were analysed during and at the end of the first growing season. Holm oak grown in phytoremediated sediments at higher concentrations showed germination levels comparable to those in the traditional substrate, and survival capacity (especially in 66% sediments) slightly higher than in 100% soil. Physiological performance of seedlings resembled that on the traditional substrate which required the addition of fertilizer, at least for the first growing season. Seedlings grown in mixed substrates with higher sediment concentrations occasionally showed better photosynthetic capacity with improved connectivity between the units of the photosystem II. At the end of the first growing season, height as well as the number of growth flushes of the seedlings grown in sole sediment or soil-sediment substrates were similar to what generally is observed for forest nursery stock of Quercus spp.. Regarding the root-system articulation and growth in depth, results in the mixed substrates were comparable to those for seedlings grown in the traditional forest nursery media, and higher than seedlings grown in 100% agronomic soil. According to our results, the reclamation of dredged sediments can provide appropriate nursery substrate for germination beds for forestry species.
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Agricultura Forestal , Sedimentos Geológicos , Árboles , Biodegradación Ambiental , Bosques , Italia , Quercus , Plantones , SueloRESUMEN
Tree mortality is a key process shaping forest dynamics. Thus, there is a growing need for indicators of the likelihood of tree death. During the last decades, an increasing number of tree-ring based studies have aimed to derive growth-mortality functions, mostly using logistic models. The results of these studies, however, are difficult to compare and synthesize due to the diversity of approaches used for the sampling strategy (number and characteristics of alive and death observations), the type of explanatory growth variables included (level, trend, etc.), and the length of the time window (number of years preceding the alive/death observation) that maximized the discrimination ability of each growth variable. We assess the implications of key methodological decisions when developing tree-ring based growth-mortality relationships using logistic mixed-effects regression models. As examples, we use published tree-ring datasets from Abies alba (13 different sites), Nothofagus dombeyi (one site), and Quercus petraea (one site). Our approach is based on a constant sampling size and aims at (1) assessing the dependency of growth-mortality relationships on the statistical sampling scheme used, (2) determining the type of explanatory growth variables that should be considered, and (3) identifying the best length of the time window used to calculate them. The performance of tree-ring-based mortality models was reasonably high for all three species (area under the receiving operator characteristics curve, AUC > 0.7). Growth level variables were the most important predictors of mortality probability for two species (A. alba, N. dombeyi), while growth-trend variables need to be considered for Q. petraea. In addition, the length of the time window used to calculate each growth variable was highly uncertain and depended on the sampling scheme, as some growth-mortality relationships varied with tree age. The present study accounts for the main sampling-related biases to determine reliable species-specific growth-mortality relationships. Our results highlight the importance of using a sampling strategy that is consistent with the research question. Moving towards a common methodology for developing reliable growth-mortality relationships is an important step towards improving our understanding of tree mortality across species and its representation in dynamic vegetation models.
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Modelos Biológicos , Árboles/fisiología , Modelos Logísticos , Análisis Multivariante , Tamaño de la MuestraRESUMEN
Tree mortality and forest dieback episodes are increasing due to drought and heat stress. Nevertheless, a comprehensive understanding of mechanisms enabling trees to withstand and survive droughts remains lacking. Our study investigated basal area increment (BAI), and δ13C-derived intrinsic water-use-efficiency (iWUE), to elucidate beech resilience across four healthy stands in Italy with varying climates and soil water availability. Additionally, fist-order autocorrelation (AR1) analysis was performed to detect early warning signals for potential tree dieback risks during extreme drought events. Results reveal a negative link between BAI and vapour pressure deficit (VPD), especially in southern latitudes. After the 2003 drought, BAI decreased at the northern site, with an increase in δ13C and iWUE, indicating conservative water-use. Conversely, the southern sites showed increased BAI and iWUE, likely influenced by rising CO2 and improved water availability. In contrast, the central site sustained higher transpiration rates due to higher soil water holding capacity (SWHC). Despite varied responses, most sites exhibited reduced resilience to future extreme events, indicated by increased AR1. Temperature significantly affected beech iWUE and BAI in northern Italy, while VPD strongly influenced the southern latitudes. The observed increase in BAI and iWUE in southern regions might be attributed to an acclimation response.
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Fagus , Agua , Dióxido de Carbono/análisis , Bosques , Árboles , Italia , Sequías , SueloRESUMEN
Wood growth is key to understanding the feedback of forest ecosystems to the ongoing climate warming. An increase in spatial synchrony (i.e., coincident changes in distant populations) of spring phenology is one of the most prominent climate responses of forest trees. However, whether temperature variability contributes to an increase in the spatial synchrony of spring phenology and its underlying mechanisms remains largely unknown. Here, we analyzed an extensive dataset of xylem phenology observations of 20 conifer species from 75 sites over the Northern Hemisphere. Along the gradient of increase in temperature variability in the 75 sites, we observed a convergence in the onset of cell enlargement roughly toward the 5th of June, with a convergence in the onset of cell wall thickening toward the summer solstice. The increase in rainfall since the 5th of June is favorable for cell division and expansion, and as the most hours of sunlight are received around the summer solstice, it allows the optimization of carbon assimilation for cell wall thickening. Hence, the convergences can be considered as the result of matching xylem phenological activities to favorable conditions in regions with high temperature variability. Yet, forest trees relying on such consistent seasonal cues for xylem growth could constrain their ability to respond to climate warming, with consequences for the potential growing season length and, ultimately, forest productivity and survival in the future.
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Tracheophyta , Temperatura , Ecosistema , Cambio Climático , Xilema , Estaciones del Año , ÁrbolesRESUMEN
Process-based models and empirical modelling techniques are frequently used to (i) explore the sensitivity of tree growth to environmental variables, and (ii) predict the future growth of trees and forest stands under climate change scenarios. However, modelling approaches substantially influence predictions of the sensitivity of trees to environmental factors. Here, we used tree-ring width (TRW) data from 1630 beech trees from a network of 70 plots established across European mountains to build empirical predictive growth models using various modelling approaches. In addition, we used 3-PG and Biome-BGCMuSo process-based models to compare growth predictions with derived empirical models. Results revealed similar prediction errors (RMSE) across models ranging between 3.71 and 7.54 cm2 of basal area increment (BAI). The models explained most of the variability in BAI ranging from 54 % to 87 %. Selected explanatory variables (despite being statistically highly significant) and the pattern of the growth sensitivity differed between models substantially. We identified only five factors with the same effect and the same sensitivity pattern in all empirical models: tree DBH, competition index, elevation, Gini index of DBH, and soil silt content. However, the sensitivity to most of the climate variables was low and inconsistent among the empirical models. Both empirical and process-based models suggest that beech in European mountains will, on average, likely experience better growth conditions under both 4.5 and 8.5 RCP scenarios. The process-based models indicated that beech may grow better across European mountains by 1.05 to 1.4 times in warmer conditions. The empirical models identified several drivers of tree growth that are not included in the current process-based models (e.g., different nutrients) but may have a substantial effect on final results, particularly if they are limiting factors. Hence, future development of process-based models may build upon our findings to increase their ability to correctly capture ecosystem dynamics.
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Ecosistema , Fagus , Cambio Climático , Bosques , ÁrbolesRESUMEN
European beech (Fagus sylvatica L.) is a widespread and economically important temperate tree species in Europe. The warmer temperatures and severe drought events expected in the future, especially in Mediterranean areas, could affect the vitality and productivity of beech stands that have been intensively used in these areas in the past. Here, we aim to assess the wood anatomical responses of beech to environmental variability and silvicultural practices by investigating three beech stands along an elevational gradient (1,200 to 1,950 m a.s.l.) in the Apennines (Italy). Therefore, we quantified several anatomical traits of the xylem vessels related to tree hydraulics from five trees per stand and investigated variability between and within tree rings. Our results suggest generally limited trait plasticity, with higher plasticity of mean vessel lumen area and theoretical hydraulic conductivity, while maximum vessel size and mean hydraulic diameter were less plastic, likely because of the stronger determination by tree height. High-elevation trees were hydraulically more limited than trees at a mid and lower elevation as indicated by the more conservative anatomical configuration, i.e., comparatively smaller vessels and a 50% tighter trait coordination. Cessation of coppicing resulted in a hydraulically safer anatomy with comparatively smaller vessels at the most intensively used site (1,200 m), triggered by increased water demand due to an increase in canopy density, and thus, an increase in stand transpiration. Furthermore, maximum vessel size at the beginning showed different climate sensitivity compared to the rest of the tree ring, while intra-ring anatomical profiles showed little difference between normal and the 5 years with the highest and lowest mean temperature and precipitation. Overall, this study highlights the challenges to separate the externally induced medium- to longer-term responses from ontogenetically determined patterns. We, therefore, call for more comprehensive studies to further explore and verify the plasticity of wood anatomical traits in European beech in response to short- to long-term environmental fluctuations to gain a mechanistic understanding useful for sustainable forest ecosystems.
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The main objectives of this research were to (i) investigate the concentration; (ii) characterize the distribution; (iii) determine the sources apportionment; (iv) estimate environmental and health risks of heavy metals in soil from mountain beech forest. A total of 76 soil samples from 20 pure beech forest stands from Bosnia and Herzegovina (BA), Bulgaria (BG), Check Republic (CZ), Germany (DE), Italy (IT), Poland (PL), Romania (RO), Serbia (RS), Slovakia (SK), Slovenia (SL), and Spain (ES) were collected. The content of major elements was measured by X-ray fluorescence spectroscopy (XRF). The content of heavy metals was measured by inductively coupled plasma-optical emission spectrometry (ICP/OES). Heavy metals had a specific concentration range, which followed in soil samples from depth 0-40 cm the common order (low to high): Hg < Cd < As < Co < Pb < Ni < Cu < Cr < Zn, and from depth 40-80 cm: Hg < Cd < As < Pb < Co < Ni < Cu < Cr < Zn. The grouping of the examined parameters according to rock types, soil types, and localities indicated the separation of carbonate rocks from other substrates, luvisol, and rendzina from other soil types, and samples from BA, SL, and IT from other localities. According to sources apportionment As, Pb and Zn are of anthropogenic origin, Cd, Co, Cr, and Ni are of geogenic origin, while the middle position of Cu and Hg indicates a combined contribution of both sources. Elements Cd and Hg indicated severe to extremely severe enrichment with a mean value of 24.3 and 70.6, respectively. Based on the determined values Ni, Cr, As and Cd do not pose a health risk.
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Fagus , Mercurio , Metales Pesados , Contaminantes del Suelo , Suelo/química , Contaminantes del Suelo/análisis , Cadmio/análisis , Plomo/análisis , Monitoreo del Ambiente/métodos , Medición de Riesgo , Metales Pesados/análisis , Bosques , Carbonatos/análisis , Mercurio/análisis , ChinaRESUMEN
Arundo donax L. is an invasive grass species with high tolerance to a wide range of environmental stresses. The response of potted A. donax plants to soil stress characterized by prolonged exposure (43 days) to salinity (+Na), to high concentration of phosphorus (+P), and to the combination of high Na and P (+NaP) followed by 14 days of recovery under optimal nutrient solution, was investigated along the entire time-course of the experiment. After an exposure of 43 days, salinity induced a progressive decline in stomatal conductance that hampered A. donax growth through diffusional limitations to photosynthesis and, when combined with high P, reduced the electron transport rate. Isoprene emission from A. donax leaves was stimulated as Na+ concentration raised in leaves. Prolonged growth in P-enriched substrate did not significantly affect A. donax performance, but decreased isoprene emission from leaves. Prolonged exposure of A. donax to + NaP increased the leaf level of H2O2, stimulated the production of carbohydrates, phenylpropanoids, zeaxanthin and increased the de-epoxidation state of the xanthophylls. This might have resulted in a higher stress tolerance that allowed a fast and full recovery following stress relief. Moreover, the high amount of ABA-glucose ester accumulated in leaves of A. donax exposed to + NaP might have favored stomata re-opening further sustaining the observed prompt recovery of photosynthesis. Therefore, prolonged exposure to high P exacerbated the negative effects of salt stress in A. donax plants photosynthetic performances, but enhanced activation of physiological mechanisms that allowed a prompt and full recovery after stress.
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Fósforo , Poaceae , Estrés Salino , Suelo , Peróxido de Hidrógeno , Fósforo/farmacología , Fotosíntesis , Hojas de la Planta/efectos de los fármacos , Poaceae/efectos de los fármacos , Estrés Salino/genética , Suelo/químicaRESUMEN
Changes of stem diameter were continuously monitored during winter in two field-grown poplar clones, using automatic point dendrometers. The objective of this study was to find an analytical solution to seasonal synchronization of stem diameter oscillations and low air temperatures. The study identified to what extent and with what frequency low air temperature induced stem diameter variation in 'Dvina' (P. deltoides) and 'I-214' (Populus x canadensis) poplar clones, after exposure to summer drought. The patterns of reversible stem shrinkage were related to the cycles of low air temperature. Hourly and daily evidence showed that 'I-214' was more sensitive to low air temperatures than 'Dvina'. The analysis of raw data and graphic details implemented with the study of derivative tests allowed an increase in the general sensitivity of the investigation applied to describe the response of poplar clones to environmental conditions. Given these diameter fluctuation patterns, automatic point dendrometers were confirmed to be a reliable non-invasive method for testing the sensitivity of diameter variation to cold temperature. Variation in rate and duration of daily stem shrinkage in response to low air temperature in winter appeared to occur independently of the effects of water deficit suffered by plants the previous summer.
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Populus/fisiología , Frío , Sequías , Tallos de la Planta/química , Tallos de la Planta/genética , Tallos de la Planta/fisiología , Populus/química , Populus/genética , Estaciones del AñoRESUMEN
The potential of Arundo donax to grow in degraded soils, characterized by excess of salinity (Na+), and phosphorus deficiency (-P) or excess (+P) also coupled with salinity (+NaP), was investigated by combining in vivo plant phenotyping, quantification of metabolites and ultrastructural imaging of leaves with a transcriptome-wide screening. Photosynthesis and growth were impaired byâ¯+â¯Na, -P andâ¯+â¯NaP. Whileâ¯+â¯Na caused stomatal closure, enhanced biosynthesis of carotenoids, sucrose and isoprene and impaired anatomy of cell walls, +P negatively affected starch production and isoprene emission, and damaged chloroplasts. Finally, +NaP largely inhibited photosynthesis due to stomatal limitations, increased sugar content, induced/repressed a number of genes 10 time higher with respect toâ¯+â¯P andâ¯+â¯Na, and caused appearance of numerous and large plastoglobules and starch granules in chloroplasts. Our results show that A. donax is sensitive to unbalances of soil ion content, despite activation of defensive mechanisms that enhance plant resilience, growth and biomass production of A. donax under these conditions.
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Fósforo/metabolismo , Poaceae/fisiología , Estrés Salino , Sodio/metabolismo , Fósforo/deficiencia , Poaceae/genética , Sodio/efectos adversos , Suelo/químicaRESUMEN
The present study investigated accumulation, translocation and tolerance of autotrophic Populus alba clone "Villafranca" in response to excess concentrations of cadmium (Cd) and copper (Cu) provided to the plants. For this purpose, increasing concentrations of Cd (0, 5, 50 and 250 µM) and Cu (0, 5, 50, 250 and 500 µM) were administered to the growth medium in which micropropagated poplar plantlets were exposed to metal treatments for 15 days. Filter bags, instead of the conventional in vitro screening, were applied to improve the experimental design. Results showed that Cd and Cu increased in shoots and roots at increasing metal concentration in the medium. The highest Cd content was found in leaves, while the highest Cu content was found in roots. In "Villafranca", Cu showed toxic effects on the development of the seedlings, especially at the highest concentrations, reducing plant dry mass. However, the tolerance index (Ti) indicated good tolerance in this clone under exposure to excess metal concentrations, whereas plants had higher translocation factor (Tf). We recommend in vitro selection of tolerant genotypes, aimed at providing early indication on accumulation potentiality and tolerance capability in research on plant sensitivity to excess heavy metal concentrations.
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Biodegradación Ambiental , Cadmio/metabolismo , Cobre/metabolismo , Populus/metabolismo , Contaminantes del Suelo/metabolismo , Cadmio/toxicidad , Cobre/toxicidad , Hojas de la Planta , Raíces de Plantas/crecimiento & desarrollo , Populus/efectos de los fármacos , Populus/crecimiento & desarrollo , Plantones , Contaminantes del Suelo/toxicidadRESUMEN
Elevation is a complex environmental factor altering temperature, light, moisture and soil nutrient availability, and thus may affect plant growth and physiology. Such effects of elevation may also depend on seasons. Along an elevational gradient of the Balang Mountain, southwestern China, we sampled soil and 2-year old leaves, 2-year old shoots, stem sapwood and fine roots (diameter<5mm) of Quercus aquifolioides at 2843, 2978, 3159, 3327, 3441 and 3589m a.s.l. in both summer and winter. In summer, the concentrations of tissue non-structural carbohydrates (NSC) did not decrease with increasing elevation, suggesting that the carbon supply is sufficient for plant growth at high altitude in the growing season. The concentration of NSC in fine roots decreased with elevation in winter, and the mean concentration of NSC across tissues in a whole plant showed no significant difference between the two sampling seasons, suggesting that the direction of NSC reallocation among plant tissues changed with season. During the growing season, NSC transferred from leaves to other tissues, and in winter NSC stored in roots transferred from roots to aboveground tissues. Available soil N increased with elevation, but total N concentrations in plant tissues did not show any clear elevational pattern. Both available soil P and total P concentrations in all plant tissues decreased with increasing elevation. Thus, tissue N:P ratio increased with elevation, suggesting that P may become a limiting element for plant growth at high elevation. The present study suggests that the upper limit of Q. aquifolioides on Balang Mountain may be co-determined by winter root NSC storage and P availability. Our results contribute to better understanding of the mechanisms for plants' upper limit formation.