RESUMEN
Mobile herbicides have a high potential for groundwater contamination. An alternative to decrease the mobility of herbicides is to apply materials with high sorbent capacity to the soil, such as biochars. The objective of this research was to evaluate the effect of eucalyptus, rice hull, and native bamboo biochar amendments on sorption and desorption of hexazinone, metribuzin, and quinclorac in a tropical soil. The sorption-desorption was evaluated using the batch equilibrium method at five concentrations of hexazinone, metribuzin, and quinclorac. Soil was amended with eucalyptus, rice hull, and native bamboo biochar at a rate of 0 (control-unamended) and 1% (w w-1), corresponding to 0 and 12 t ha-1, respectively. The amount of sorbed herbicides in the unamended soil followed the decreasing order: quinclorac (65.9%) > metribuzin (21.4%) > hexazinone (16.0%). Native bamboo biochar provided the highest sorption compared to rice hull and eucalyptus biochar-amended soils for the three herbicides. The amount of desorbed herbicides in the unamended soil followed the decreasing order: metribuzin (18.35%) > hexazinone (15.9%) > quinclorac (15.1%). Addition of native bamboo biochar provided the lowest desorption among the biochar amendments for the three herbicides. In conclusion, the biochars differently affect the sorption and desorption of hexazinone, metribuzin, and quinclorac mobile herbicides in a tropical soil. The addition of eucalyptus, rice hull, and native bamboo biochars is a good alternative to increase the sorption of hexazinone, metribuzin, and quinclorac, thus, reducing mobility and availability of these herbicides to nontarget organisms in soil.
Asunto(s)
Eucalyptus , Herbicidas , Oryza , Quinolinas , Sasa , Contaminantes del Suelo , Triazinas , Carbón Orgánico , Suelo , Adsorción , Monitoreo del Ambiente , Herbicidas/análisis , Contaminantes del Suelo/análisisRESUMEN
Land use and management changes affect the composition and diversity of soil bacteria and fungi, which in turn may alter soil health and the provision of key ecological functions, such as pesticide degradation and soil detoxification. However, the extent to which these changes affect such services is still poorly understood in tropical agroecosystems. Our main goal was to evaluate how land-use (tilled versus no-tilled soil), soil management (N-fertilization), and microbial diversity depletion [tenfold (D1 = 10-1) and thousandfold (D3 = 10-3) dilutions] impacted soil enzyme activities (ß-glycosidase and acid phosphatase) involved in nutrient cycles and glyphosate mineralization. Soils were collected from a long-term experimental area (35 years) and compared to its native forest soil (NF). Glyphosate was selected due to its intensive use in agriculture worldwide and in the study area, as well as its recalcitrance in the environment by forming inner sphere complexes. Bacterial communities played a more important role than the fungi in glyphosate degradation. For this function, the role of microbial diversity was more critical than land use and soil management. Our study also revealed that conservation tillage systems, such as no-tillage, regardless of nitrogen fertilizer use, mitigates the negative effects of microbial diversity depletion, being more efficient and resilient regarding glyphosate degradation than conventional tillage systems. No-tilled soils also presented much higher ß-glycosidase and acid phosphatase activities as well as higher bacterial diversity indexes than those under conventional tillage. Consequently, conservation tillage is a key component for sustaining soil health and its functionality, providing critical ecosystem functions, such as soil detoxification in tropical agroecosystems.
Asunto(s)
Ecosistema , Suelo , Microbiología del Suelo , Agricultura , Bacterias/genética , Bacterias/metabolismo , GlifosatoRESUMEN
Deltamethrin is an insecticide with high toxicity to non-target aquatic organisms. Environment-friendly alternatives to removing insecticides from water bodies, like phytoremediation, require species to uptake and/or dissipate pesticides from water. Our research investigated the ability of Egeria densa plants to absorb and dissipate 14C-deltamethrin from water, and bioaccumulation in Danio rerio. The variables were four densities of E. densa (0, 234, 337, and 468 g dry weight m-3), in tanks with seven adults of D. rerio, with three replicates. Dissipation was evaluated at 0, 24, 48, 72, and 96 h after application (HAA). After 96 HAA, the uptake of 14C-deltamethrin by plants and accumulation in fish were assessed. The E. densa increased 14C-deltamethrin dissipation and reduced bioaccumulation in zebrafish. The DT50 decreased 3-fold in treatments with 337 and 468 g m-3 of E. densa. The plants absorbed 32% of the 14C-deltamethrin applied, regardless of plant density. The bioaccumulation in fish was 8.21% without E. densa and only 1% in treatments with 468 g m-3 of plants. These results suggest phytoremediation using E. densa is a possible alternative to removing deltamethrin from water and reducing the accumulation in non-target organisms, reducing the environmental impact of insecticides in aquatic ecosystems.
Asunto(s)
Insecticidas , Contaminantes Químicos del Agua , Animales , Pez Cebra , Insecticidas/toxicidad , Ecosistema , Bioacumulación , Contaminantes Químicos del Agua/toxicidad , AguaRESUMEN
Witches' broom disease of cacao is caused by the pathogenic fungus Moniliophthora perniciosa. By using tomato (Solanum lycopersicum) cultivar Micro-Tom (MT) as a model system, we investigated the physiological and metabolic consequences of M. perniciosa infection to determine whether symptoms result from sink establishment during infection. Infection of MT by M. perniciosa caused reductions in root biomass and fruit yield, a decrease in leaf gas exchange, and down-regulation of photosynthesis-related genes. The total leaf area and water potential decreased, while ABA levels, water conductance/conductivity, and ABA-related gene expression increased. Genes related to sugar metabolism and those involved in secondary cell wall deposition were up-regulated upon infection, and the concentrations of sugars, fumarate, and amino acids increased. 14C-glucose was mobilized towards infected MT stems, but not in inoculated stems of the MT line overexpressing CYTOKININ OXIDASE-2 (35S::AtCKX2), suggesting a role for cytokinin in establishing a sugar sink. The up-regulation of genes involved in cell wall deposition and phenylpropanoid metabolism in infected MT, but not in 35S::AtCKX2 plants, suggests establishment of a cytokinin-mediated sink that promotes tissue overgrowth with an increase in lignin. Possibly, M. perniciosa could benefit from the accumulation of secondary cell walls during its saprotrophic phase of infection.
Asunto(s)
Agaricales , Cacao , Solanum lycopersicum , Agaricales/genética , Cacao/genética , Pared Celular , Citocininas , Solanum lycopersicum/genética , Solanum lycopersicum/microbiología , Enfermedades de las Plantas/microbiología , Azúcares , AguaRESUMEN
Sulfometuron-methyl is a broad-spectrum herbicide, used throughout Brazil; however, its environmental impacts in biochar (BC) amended soils is not fully understood. Biochar is known to enhance soil quality but can also have undesired effects such as altering the bioavailability and behavior of herbicides. Microbial communities can degrade herbicides such as sulfometuron-methyl in soils; however, they are known to be affected by BC. Therefore, it is important to understand the tripartite interaction between these factors. This research aimed to evaluate the sorption-desorption and biodegradation of sulfometuron-methyl in Amazonian soils amended with BC, and to assess the effects of the interactions between BC and sulfometuron-methyl on soil bacterial communities. Soil samples were collected from field plots amended with BC at three doses (0, 40 and 80 t ha-1) applied ten years ago. The herbicide sorption and desorption were evaluated using a batch equilibrium method. Mineralization and biodegradation studies were conducted in microcosms incubated with 14C-sulfometuron-methyl for 80 days. Systematic soil sampling, followed by DNA extraction, quantification (qPCR) and 16S rRNA amplicon sequencing were performed. The presence of BC increased the sorption of the herbicide to the soil by 11% (BC40) and 16% (BC80) compared to unamended soil. The presence of BC also affected the degradation of 14C-sulfometuron-methyl, reducing the mineralization rate and increasing the degradation half-life times (DT50) from 36.67 days in unamended soil to 52.11 and 55.45 days in BC40 and BC80 soils, respectively. The herbicide application altered the bacterial communities, affecting abundance and richness, and changing the taxonomic diversity (i.e., some taxa were promoted and other inhibited). A tripartite interaction was found between BC, the herbicide and soil bacterial communities, suggesting that it is important to consider the environmental impact of soil applied herbicides in biochar amended soils.
Asunto(s)
Biodegradación Ambiental , Herbicidas/análisis , Contaminantes del Suelo/análisis , Compuestos de Sulfonilurea/análisis , Adsorción , Bacterias/metabolismo , Disponibilidad Biológica , Brasil , Carbón Orgánico , ARN Ribosómico 16S/metabolismo , SueloRESUMEN
Quinclorac and tebuthiuron are residual herbicides that may remain in the soil longer than for the cropping season. The objective of this research was to evaluate the use of green manure plants to remediate soils treated with quinclorac and tebuthiuron. Soils were separately treated with 14C-quinclorac and 14C-tebuthiuron at 266.4 and 132 g ha-1, respectively. After 21 days, four green manure plants, namely Crotalaria spectabilis, Canavalia ensiformis, Stizolobium aterrimum, and Lupinus albus, were separately sown in the treated soils. Overall, all four species absorbed more 14C-tebuthiuron [C. ensiformes (22.49%), S. aterrimum (16.71%), L. albus (15%), and C. spectabilis (4.48%)] than 14C-quinclorac [C. ensiformis (13.44%), L. albus (10.02%), S. aterrimum (6.2%), and C. spectabilis (1.75%)]. Quinclorac translocation in all four plants was greater in young leaves compared to old leaves, cotyledons, or roots, and 14C-tebuthiuron translocation in all four plant species was greater in old leaves and cotyledons compared to young leaves or roots. Regardless of the differences in translocation between the two herbicides, the four green manure plants are capable to remediate soils that have been treated with quinclorac and tebuthiuron. However, C. ensiformis is more efficient for the remediation of tebuthiuron-treated soil compared to the other plants.
Asunto(s)
Herbicidas , Contaminantes del Suelo , Biodegradación Ambiental , Estiércol , Compuestos de Metilurea , Quinolinas , Suelo , Contaminantes del Suelo/análisisRESUMEN
Saflufenacil is an herbicide that is leachable in soil and has the potential to contaminate groundwater, besides having moderate toxicity to aquatic organisms. Some macrophyte species may interfere with the availability of herbicides in water, increasing dissipation in this environment. Thus, the objective of this work was to evaluate the absorption and dissipation of 14C-saflufenacil in water by Egeria densa and Pistia stratiotes. Dissipation was performed with 14C-saflufenacil applied directly in water and quantified by liquid scintillation spectrometry (LSS). The evaluation times were 0, 3, 6, 24, 48, 72 and 96 h after application (HAA) for E. densa and 0, 12, 24, 36, 48, 60, 84 and 108 HAA for P. stratiotes. Absorption was analyzed through plant combustion in a biological oxidizer. The presence of the macrophytes increased the dissipation of 14C-saflufenacil in water. The half-life time (DT50) of the herbicide decreased by 82.6% in the presence of E. densa at 96 HAA. For P. stratiotes, the reduction in DT50 was 94.8% at 108 HAA. The absorption of 14C-saflufenacil was low for both macrophytes during the evaluated time. However, the macrophytes E. densa and P. stratiotes showed potential for the phytoremediation of water contaminated with saflufenacil.
Asunto(s)
Araceae , Contaminantes Químicos del Agua , Biodegradación Ambiental , Pirimidinonas , Sulfonamidas , Agua , Contaminantes Químicos del Agua/análisisRESUMEN
This study aimed to characterize the effect of amending soils with biochars derived from soybean residues, sugarcane bagasse, and wood chips on the sorption-desorption of indaziflam and indaziflam-triazinediamine (FDAT), indaziflam-triazine-indanone (ITI), and indaziflam-carboxylic acid (ICA) metabolites applied to soils from three Midwestern U.S. states, a silt loam and a silty clay loam. Biochars produced from different feedstock were used as soil amendments and compared with raw feedstock. Sorption-desorption experiments of indaziflam and its three metabolites were performed using the batch equilibration method and analyzed for 14C activity by liquid scintillation counting (radiometric technique). In all soils, the use of organic amendments promoted greater sorption and less desorption of indaziflam and ITI. The addition of biochar to soils promoted greater sorption of the four tested chemical products compared with the corresponding raw materials. Among the biochars, grape wood chips showed greater potential in sorb indaziflam and ITI. In general, none of the biochars affected the sorption and desorption of FDAT and ICA. Characterization of biochar to be used as a soil amendment (immobilizer) is highly recommended prior to field addition to optimize the sorption process and to prevent increased soil and water contamination of indaziflam and its metabolites following biochar addition.
Asunto(s)
Herbicidas , Contaminantes del Suelo , Adsorción , Carbón Orgánico , Herbicidas/análisis , Indenos , Suelo , Contaminantes del Suelo/análisis , TriazinasRESUMEN
Fertiactyl® is a foliar fertilizer with the potential to minimize the phytotoxicity effects caused by glyphosate drift in eucalyptus plants. As the interactions of the glyphosate and Fertiactyl® in tank mix on the plant behavior are not yet known, the objective was to evaluate the absorption and translocation of 14C-glyphosate, applied isolated and mixed in tank with Fertiactyl®, in young eucalyptus plants (clone I-144, Eucalyptus urophylla x E. grandis). The addition of Fertiactyl® to the mixture of 14C-glyphosate reduced the absorption by 94.3% in relation to the total absorbed at the end of the evaluation compared to plants treated only with 14C-glyphosate, i.e., Fertiactyl® protected the eucalyptus plants of the glyphosate intoxication by drift. The translocation rates from the treated leaves to the rest of the shoots and roots were low (<2% of the total recovered) in both treatments, suggest that restricted translocation is a mechanism of natural tolerance to glyphosate in plants of clone I-144. It is concluded that Fertiactyl®, mixed in the solution with glyphosate, protects young eucalyptus plants against glyphosate drift by reducing the amount of herbicide absorbed.
Asunto(s)
Eucalyptus/efectos de los fármacos , Fertilizantes , Glicina/análogos & derivados , Herbicidas/farmacocinética , Radioisótopos de Carbono/farmacocinética , Eucalyptus/metabolismo , Glicina/farmacocinética , Glicina/toxicidad , Herbicidas/toxicidad , Hojas de la Planta/efectos de los fármacos , Brotes de la Planta/efectos de los fármacos , Distribución Tisular , GlifosatoRESUMEN
The application of glyphosate to coffee crops can cause injuries to plants. Fertiactyl® foliar fertilizer reduces injuries when mixed with glyphosate; however, it is important to establish which mechanisms are responsible for this protective action. This study aimed to evaluate the absorption and translocation of glyphosate applied separately and in mixture with Fertiactyl® in coffee seedlings. Absorption and translocation were performed with 14C-glyphosate applied separately and in mixture with Fertiactyl® at 0, 6, 12, 24, 48, 96, and 144 hours after application (HAA). Most of the 14C-glyphosate applied to coffee seedlings was not absorbed. The 14C-glyphosate applied separately had a higher absorption by coffee seedlings (6.5%) than in a mixture with Fertiactyl® (2.7%) at 144 HAA. The maximum translocation of the 14C-glyphosate applied separately was 0.69% at 81.2 HAA and in mixture with Fertiactyl® was 0.41% at 41.2 HAA. The treated leaves retained a higher percentage of 14C-glyphosate when applied separately (5.6% at 144 HAA) than in a mixture with Fertiactyl® (2.2% at 144 HAA). Low translocation (<1%) for the rest of the plant shoots was observed both for the 14C-glyphosate applied separately and in combination with Fertiactyl®. Therefore, Fertiactyl® decreased the absorption and translocation of 14C-glyphosate in coffee seedlings.
Asunto(s)
Coffea/metabolismo , Fertilizantes , Glicina/análogos & derivados , Herbicidas/farmacocinética , Absorción Fisiológica , Transporte Biológico , Glicina/farmacocinética , Hojas de la Planta/metabolismo , Plantones/metabolismo , GlifosatoRESUMEN
Glyphosate residues in grain can be explained by the concentrations and formulations of glyphosate products. This study aimed to evaluate the residues from glyphosate formulations labeled with 14C-glyphosate applied to leaves of glyphosate-resistant soybean (GRS) in two life cycles by liquid scintillation spectrometry. Different plant tissues were analyzed after the end of the plants' life cycles. The experimental design was four repetitions of three treatments: Roundup® Original, Roundup Ready®, and unformulated glyphosate (control). The application of the dosing solution was 120 µL on the first four trifoliate leaves (10 µL per leaflet) of each plant, deposited manually with a 1-µL dispenser. All treatment solutions were calculated at a 1.2 kg a.e ha-1 of glyphosate. Glyphosate formulations of Roundup® Original and Roundup Ready® increased 14C-glyphosate distribution in GRS compared to the unformulated herbicide, regardless of the experiment (first or second cycle). Overall, the percentages of total radioactivity applied (18.33 kBq) found in grains were less than 5%. Grains, stems, and leaves showed the highest levels of herbicide residues compared to other parts of the plant. Despite the Roundup Ready® formulation having increased residues, the highest value found in grains, 1.95 mg kg-1, was less than 10 mg kg-1, the maximum residue limit (MRL) in Brazil.
Asunto(s)
Glycine max , Herbicidas , Brasil , Glicina/análogos & derivados , GlifosatoRESUMEN
Sugarcane straw may work as a physical barrier for pre-emergent herbicides and interact with their molecules, increasing sorption process. Adjuvants may change herbicides dynamics in the environment and improve their efficiency for weed control. The objective of this work was to evaluate sorption and desorption of pendimethalin alone and in mixture with adjuvant in soil and sugarcane straw. Sorption experiments were performed using pendimethalin alone and in mixture with vegetable oil with herbicide solution concentrations ranging between 2.5 and 40 µg mL-1 for both conditions. Sorption distribution coefficient (Kd) for soil was 18.48 mL g-1 using pendimethalin alone. Kd value was not determined when pendimethalin was in mixture with adjuvant due to the complete retention of the herbicide in the soil regardless of the initial aqueous phase concentration. Sugarcane straw sorption experiment had Kd values corresponding to 355.52 and 27.24 mL g-1 for pendimethalin alone and in mixture with adjuvant, respectively, indicating the addition of vegetable oil may significantly decrease pendimethalin retention in the straw and could improve weed control. Besides all desorption coefficients were higher than the respective sorption coefficients, which means that the sorption process may be considered irreversible.
Asunto(s)
Compuestos de Anilina/química , Herbicidas/química , Saccharum , Suelo/química , Adsorción , Brasil , Cromatografía Líquida de Alta Presión , Tallos de la Planta/química , Agua , Control de Malezas/métodosRESUMEN
The study was to identify the potential tolerance of Crotalaria juncea to diclosulam uptake and translocation and its effects on the physiological metabolism of plants. Two experiments were carried out; I-Evaluation of uptake and translocation of 14C-diclosulam (35 g a.i. ha-1) in C. juncea, at seven and 14 days after emergence. II-Evaluation of chlorophyll a transient fluorescence of dark-adapted C. juncea leaves when applied diclosulam in pre-emergence. Plants of C. juncea presented an anatomical/metabolic barrier to diclosulam translocation in the stem, which may confer tolerance to this herbicidal, besides reduced translocation due to low accumulation in the cotyledons. In addition, plants can maintain photosynthetic metabolism active when growing in soil with diclosulam by not changing the dynamics of energy dissipation. Thus, when cultivated in soil with residual of diclosulam, C. juncea can tolerate the herbicide to maintain plant growth.
Asunto(s)
Crotalaria/fisiología , Herbicidas/metabolismo , Sulfonamidas/metabolismo , Triazoles/metabolismo , Transporte Biológico , Clorofila A , Hojas de la Planta/metabolismo , SueloRESUMEN
Atrazine is an herbicide that is applied in corn around the world and in sugarcane in Brazil. It is known to be hazardous for animals' health, mobile in the soil, and its analysis is considered expensive and onerous. Solute movement studies are essential to provide information about dangerous molecules movement, which can avoid contamination. While field investigations demand time and financial resources, numerical models are an alternative to describe water and solute distribution in the soil profile. Thus, the objective of this work was to use HYDRUS 2-D model for simulations of atrazine movement in containers packed with tropical soil cultivated with corn and to compare simulated and observed data through statistical parameters. The research was carried out in a greenhouse during 116 days after planting. Atrazine was analyzed in the soil solution at three different depths to validate HYDRUS-2D. Simulations were carried out using hydraulic properties fitted directly to measured retention data and parameters for corn growing and atmospheric characteristics. The mixed procedure analysis indicated that there are differences in atrazine concentration among depths and along time. In general, atrazine concentration is higher at shallow depths and right after application. However, it is possible to find atrazine in deeper soil layers, which might be a concern regarding contamination. RMSE, Willmott and Pearson coefficients indicated a favorable capacity of the model to simulate atrazine concentration on corn cultivation. HYDRUS-2D is a reliable tool to obtain trends in atrazine movement under these experiment's conditions. The uptake parameters, the crop root growth and distribution parameters depend on further specific studies to better describe the relationship between the plant and atrazine and meteorological parameters need to be updated.
Asunto(s)
Atrazina , Herbicidas , Contaminantes del Suelo , Animales , Brasil , Suelo , Zea maysRESUMEN
After application, herbicides often reach the soil and affect non-target soil microorganisms, decreasing their population, diversity or affecting metabolic activity. Therefore, laboratory studies were performed to evaluate the effects of diuron, hexazinone and sulfometuron-methyl alone and mixed upon carbon transformation by soil microorganisms in clayey and sandy soils and the effect on bacterial diversity and structure. Control treatment without herbicide application was also performed. Sub-samples from the control and herbicide treatments (10 g - in triplicate) were collected before herbicide application and 7, 14, 28 and 42 days after treatment (DAT), then 1 mL of 14C-glucose solution was applied. The released 14CO2 was trapped in 2 M NaOH solution and the radioactivity was analyzed by liquid scintillation counting (LSC), 12 h after glucose application. The effect of herbicides on bacterial diversity was evaluated by T-RFLP. The experiment was conducted in a complete randomized design. Hexazinone did not affect 14CO2 evolution. Diuron showed a greater 14CO2 evolution in sandy and clayey soil, while sulfometuron-methyl led to an increase in sandy soil, at 42 DAT. A greater evolution of carbon was observed in the treatment with herbicide mixture in sandy soil, compared with the same treatment in clayey soil or control. However, the herbicide mixture application did not affect the soil biological activity measured by the respiration rate induced by substrate. On the other hand, the herbicide mixtures affected the bacterial diversity in both soils, being the strongest effect to diuron and sulfometuron-methyl in clayey soil and hexazinone in sandy soil.
Asunto(s)
Bacterias/efectos de los fármacos , Diurona/toxicidad , Microbiología del Suelo , Compuestos de Sulfonilurea/toxicidad , Triazinas/toxicidad , Bacterias/metabolismo , Carbono/metabolismo , Dióxido de Carbono/metabolismo , Herbicidas/toxicidad , Polimorfismo de Longitud del Fragmento de Restricción , Suelo/química , Contaminantes del Suelo/toxicidadRESUMEN
In Brazil, weed management in sugarcane fields is mainly done with the use of selective herbicide formulations. For many years, diuron+hexazinone was one of the main herbicide mixture formulations used in sugarcane. Later, sulfometuron-methyl was included in the same mixture, which was marketed as a new herbicide formulation for residual in-season weed control in sugarcane. The mixture diuron+hexazinone+sulfometuron-methyl has been widely used in commercial sugarcane fields in Brazil. However, recent field observations have shown that sugarcane plants at different growth stages varied in their phytotoxicity levels after treatment with diuron+hexazinone+sulfometuron-methyl. Greenhouse and laboratory studies were conducted to determine 14Csulfometuron-methyl absorption and translocation, as well as 14C distribution in sugarcane at two growth stages, 2 to 3 leaves and 5 to 6 leaves. 14Csulfometuron-methyl absorption by sugarcane did not differ between the two growth stages. Different patterns of 14C accumulation were observed, which may explain variations in sulfometuron-methyl phytotoxic responses observed in the field.
Asunto(s)
Herbicidas/farmacocinética , Saccharum/efectos de los fármacos , Saccharum/crecimiento & desarrollo , Compuestos de Sulfonilurea/farmacocinética , Brasil , Radioisótopos de Carbono/farmacocinética , Saccharum/metabolismoRESUMEN
This study aimed to evaluate the sorption-desorption and leaching of aminocyclopyrachlor from three Brazilian soils. The sorption-desorption of 14C-aminocyclopyrachlor was evaluated using the batch method and leaching was assessed in glass columns. The Freundlich model showed an adequate fit for the sorption-desorption of aminocyclopyrachlor. The Freundlich sorption coefficient [Kf (sorption)] ranged from 0.37 to 1.34 µmol (1-1/n) L1/n kg-1 and showed a significant positive correlation with the clay content of the soil, while the Kf (desorption) ranged from 3.62 to 5.36 µmol (1-1/n) L1/n kg-1. The Kf (desorption) values were higher than their respective Kf (sorption), indicating that aminocyclopyrachlor sorption is reversible, and the fate of this herbicide in the environment can be affected by leaching. Aminocyclopyrachlor was detected at all depths (0-30 cm) in all the studied soils, where leaching was influenced by soil texture. The total herbicide leaching from the sandy clay and clay soils was <0.06%, whereas, â¼3% leached from the loamy sand soil. The results suggest that aminocyclopyrachlor has a high potential of leaching, based on its low sorption and high desorption capacities. Therefore, this herbicide can easily contaminate underground water resources.
Asunto(s)
Pirimidinas/química , Contaminantes del Suelo/química , Adsorción , Silicatos de Aluminio , Brasil , Arcilla , Herbicidas/análisis , Herbicidas/química , Pirimidinas/análisis , Suelo/química , Contaminantes del Suelo/análisis , Contaminantes Químicos del Agua/químicaRESUMEN
The use of pesticides is a widely spread practice in Brazilian agriculture, and dispersion of these substances is an important factor for the fauna and flora. Atrazine is an endocrine disruptor in the xenoestrogen class that is used worldwide in agricultural practices. In Brazil, its use is permitted in several crops. Podocnemis expansa is a representative of the Testudines order that is the largest freshwater reptile of South America. Its distribution enables it to get in contact with molecules that are commonly used as pesticides, which may cause deleterious effects in target populations. In order to evaluate the possible effects of the exposure to atrazine on bone ontogeny of this species, eggs were artificially incubated in sand moistened with water contaminated with atrazine at concentrations equal to 0, 2, 20 or 200 µg/L. Embryos were collected throughout incubation and submitted to diaphanization of soft tissues with potassium hydroxide (KOH); bones were stained with Alizarin red S and cartilages by Alcian blue. Embryos were evaluated for the presence of abnormalities during the different stages of pre-natal development of skeletal elements. No effect of atrazine was observed on bone development during the embryonic phase in P. expansa individuals, in the conditions of this study.
Asunto(s)
Atrazina/toxicidad , Desarrollo Óseo/efectos de los fármacos , Monitoreo del Ambiente , Herbicidas/toxicidad , Tortugas , Contaminantes Químicos del Agua/toxicidad , Animales , BrasilRESUMEN
An on-line solid phase extraction liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method for the determination of 12 antimicrobials in sediment and surface water was developed and validated. Furthermore, the spatial and temporal antimicrobials distributions in the sediment and in the water of four fish farms located in the hydroelectric dam of Ilha Solteira Reservoir in Brazil were investigated over four seasons in three sampling sites: at the fish cages, 100 and 1,000 m downstream far from the cages. The method was performed using an Agilent Zorbax 80 SB-C8 column (9.4 × 15 mm, 5 µm) as the loading column, and the Agilent Zorbax Eclipse Plus C18 column (3.0 × 100 mm, 3.5 µm) as a separation column within a run time of 13 min. The limits of quantification were less than 9 ng·L(-1) for the antibiotics in water and 16 µg·kg(-1) in sediment; the recovery ranged from 80 to 119%, with a variation coefficient less than 11%, and the repeatability was lower than 15%. Oxytetracycline was found in the water in all sample seasons. However, florfenicol was identified in April and October 2013 and January 2014, and tetracycline was present in July 2013. Regarding the sediment, oxytetracycline and tetracycline were found in all sampling periods, but chlortetracycline was only identified in January 2014. The spatial distribution of antimicrobials showed that the main pollution source came from the fish farms. This study demonstrated that the proposed method is reliable for the monitoring of antimicrobials in water and sediments and it showed contamination in both matrices from Ilha Solteira Reservoir.
Asunto(s)
Antibacterianos/análisis , Monitoreo del Ambiente/métodos , Explotaciones Pesqueras , Sedimentos Geológicos/química , Contaminantes Químicos del Agua/análisis , Agua/química , Brasil , Cromatografía Liquida/métodos , Estaciones del Año , Extracción en Fase Sólida/métodos , Análisis Espacio-Temporal , Espectrometría de Masas en Tándem/métodos , Factores de TiempoRESUMEN
The aim of this study was to investigate the relationship between antibiotic residues found in the muscle of cage-farm-raised Nile tilapia (Oreochromisniloticus), the occurrence of resistant bacteria, and the sanitary practices adopted by farmers in Ilha Solteira reservoir, Brazil. Nine fish (three small fish, 40-200 g; three medium-sized fish, 200-500 g; and three large fish, 500-800 g) were collected from four cage farms every three months from April 2013 to January 2014. Ten antibiotic residues were determined using liquid chromatography-mass spectrometry, and bacteria were isolated and tested for antibiotic resistance to determine the multiple antibiotic resistance (MAR) index. Only three antibiotics (oxytetracycline, tetracycline, and florfenicol) were detected in the muscle of Nile tilapia, and their residues were the highest in small fish; however, the MAR index was higher in large fish. In addition, a direct positive relationship between the MAR index and the concentration of antibiotic residues in Nile tilapia was found. Overall, the adoption of prophylactic management practices improved the sanitary status of cage farms, reducing bacterial infections and hampering the development of antibiotic-resistant bacteria.