High School Girls' Volleyball Athletes' Self-Reported Management of Pain, Intentions to Report Overuse Injuries, and Intentions to Adhere to Medical Advice for Treating Overuse Injuries.

CONTEXT: Girls' high school volleyball is a popular sport with a high rate of overuse injuries and sport specialization. Health professionals perceive that high school athletes are reluctant to follow treatment plans involving sport activity reduction. This study's purpose was to describe high school girls' volleyball athletes' self-reported shoulder and knee pain, the likelihood of adhering to medical advice, and the association of factors that influence the likelihood of reporting overuse injuries and sport specialization. STUDY DESIGN: Cross-sectional. METHODS: Participants completed an online survey (demographics, sport participation measures, shoulder and knee pain information, medical adherence likelihood [4-point Likert: not at all likely to extremely likely], and factors influencing overuse injury reporting intentions). A 2 × 2 chi-square analysis compared factors that influence athletes' intentions to "not report an overuse injury" (eg, I thought my coach would get mad; yes/no) and sport specialization (nonhighly specialized/highly specialized athletes). RESULTS: There were 150 participants (highly specialized = 56%, grade: ninth = 33%, 10th = 28%, 11th = 22%, 12th = 17%). At least 60% reported shoulder and knee pain related to an overuse mechanism. Most reportedly did not seek rehabilitation led by a medical provider (shoulder pain = 66%, knee pain = 60%). Only 11% of athletes reported they were "extremely likely" to rest from sporting activity during the regular season if advised by a medical professional. Highly specialized athletes were more likely to report the pursuit of a college scholarship as a factor that influences their intention to report an overuse injury compared to nonhighly specialized athletes (13% vs 3%, respectively, P = .04). CONCLUSIONS: Most girls' volleyball athletes did not treat their pain with guided rehabilitation, which may increase their risk of a worse overuse injury or even acute injury. Clinicians, athletes, parents, and coaches need to work together to create a sport culture that empowers athletes to discuss their pain and overuse injuries with medical professionals.

Effects of Kinesio Taping on Tuck Jump Performance in Competitive Male Athletes.

This study aimed to measure the acute effects of kinesio taping at different stretches on tuck jump performance in active individuals. Seventy-five males (23.01±2.24 years, 178.35±8.12 cm, 72.47±6.58 kg) were randomly distributed into three groups: (1) kinesio taping without stretch, (2) kinesio taping with approximately 50% stretch, and (3) kinesio taping with approximately 75% stretch. The tuck jump performance of all participants was determined at baseline, immediately after applying kinesio taping, and 24 and 72 hours later. The participants' tuck jump performance did not improve immediately after the kinesio taping application, 24 hours, and 72 hours after the application. No significant differences were found between the kinesio taping groups at any time point (p>0.05). This study demonstrated no effects of kinesio taping on neuromuscular performance.

Contributions of Lower-Body Strength Parameters to Critical Power and Anaerobic Work Capacity.

ABSTRACT: Byrd, MT, Wallace, BJ, Clasey, JL, and Bergstrom, HC. Contributions of lower-body strength parameters to critical power and anaerobic work capacity. J Strength Cond Res 35(1): 97-101, 2021-This study examined the contribution of lower-body strength and isokinetic peak torque measures to the prediction of critical power (CP) and anaerobic work capacity (AWC). Fourteen recreationally trained males (mean ± SD age: 22.4 ± 2.5 years; height: 177.9 ± 7.7 cm; body mass: 84.2 ± 12.4 kg) with anaerobic training experience participated in this study. The lower-body strength measures included 1 repetition max bilateral back squat (BSq), isokinetic peak torque at 30°·s-1 [PT30], and isokinetic peak torque at 240°·s-1 [PT240] of the dominant leg. The CP and AWC were determined from the 3-minute all-out CP cycle ergometer test (CP3MT), with the resistance set at 4.5% of the total body mass. The CP was defined as the mean power output over the final 30 seconds of the test, and the AWC was calculated using the equation, AWC = 150 seconds (P150 - CP), where P150 equals the mean power output for the first 150 seconds. Stepwise regression analyses indicated that only BSq contributed significantly to the prediction of AWC (AWC = 0.0527 [BSq] + 8.094 [standard error of estimate = 2.151 kJ; p = 0.012]), with a correlation of r2 = 0.423. None of the strength parameters significantly predicted CP. These findings indicated that BSq strength accounted for 42% of the variance in AWC, but lower-body strength was not related to CP. The current results indirectly support the unique metabolic characteristics of both CP and AWC in providing separate measures of an individual's aerobic and anaerobic capabilities, respectively.

Squat Muscle Activation Patterns with Hip Rotations in Subjects with Genu Varum Deformity.

The purpose of this study was to compare muscle activation during the squat with different hip rotations (neutral, 15, 30, and 45° of internal and external hip rotation) in subjects with and without Genu Varum deformity deformity. Surface electromyography were recorded from 32 men with (n=16) and without (n=16) Genu Varum deformity. In the Genu Varum deformity group, the squats with 30, 45 and 15° of internal rotations of the hip showed significantly greater gluteus medius activation as compared to other positions. Moreover, the tensor fascia lata activity increased with greater external rotation of the hip, and significantly more than hip internal rotations (p<0.05). For vastus medialis and vastus lateralis, both hip internal and external rotation showed a significantly greater activation compared to the neutral hip positions (p<0.05). There were significant differences in the gluteus medius:tensor fascia lata activity ratio (p=0.001) and the vastus medialis: vastus lateralis activity ratio (p=0.001) between the different hip positions in the Genu Varum deformity and healthy groups. These results demonstrate that muscle activation patterns varied significantly with the position of different hip rotation in both groups. Those with Genu Varum deformity may use this information to aid in an injury prevention strategy by choosing squat positioning that favorably alters muscle activation patterns.

Effects of Relative Drop Heights of Drop Jump Biomechanics in Male Volleyball Players.

Previous cross-sectional studies have reported that higher drop heights do not always result in improved performance, and may increase injury risk during drop jumps (DJ). The purpose of this study was to analyze the kinematics and kinetics during the DJ in order to determine the relative drop height that maximize performance without exposing the lower extremity joints to unnecessary loads. Twenty male Division I college volleyball players volunteered. Data were collected using 11 infrared cameras and two force platforms. Participants performed three maximal effort countermovement jumps (CMJ). Subsequently, 50, 75, 100, 125, and 150% CMJ height (CMJH) was used to scale their relative drop height for three DJ trials per height. There was a significant increase in the landing phase impulse when the drop height exceeded 100%CMJH (p<0.05). At 125% and 150%CMJH, the negative work of knee and ankle significantly increased. The incoming velocity, kinetic energy, landing depth, maximum ground reaction force, landing impulse and power absorption of knee and ankle all increased with drop height (p<0.05). DJ height and reactive strength index following the drop landing were not statistically different between any of the drop heights (p>0.05). 50% to 100%CMJH may be the appropriate individual relative drop height for the DJ.

Muscular and Neural Contributions to Postactivation Potentiation.

Wallace, BJ, Shapiro, R, Wallace, KL, Abel, MG, and Symons, TB. Muscular and neural contributions to postactivation potentiation. J Strength Cond Res 33(3): 615-625, 2019-This study's purpose was to explain the relationship between muscle factors (twitch potentiation [TP]) and neural factors (reflex potentiation) contributing to total postactivation potentiation (PAP) that couples these. The tibial nerve of 15 participants were stimulated intermittently for 20 minutes at supramaximal (Mmax) and submaximal (Hmax) intensities on separate days under 2 conditions: (a) rest (Control) and (b) after a 10-second plantarflexion maximum voluntary isometric contraction (MVIC). Isometric twitch torque and rate of force development (RFD) as well as soleus and gastrocnemius electromyographic values were analyzed. Torque and RFD TP were significantly greater 10 and 30 seconds after MVIC vs. Control. Postactivation potentiation of torque and RFD at Hmax were highest at 3 and 4.5 minutes after MVIC, respectively, with RFD significantly elevated. Electromyographic values were not different between conditions. Twitch potentiation significantly contributed to PAP at the following time points: 20 seconds, Hmax peak, and 20 minutes after MVIC (torque: R = 0.54, 0.76, and 0.70; RFD: R = 0.46, 0.59, and 0.53). The soleus significantly contributed to PAP torque at 20 seconds and 20 minutes after MVIC, and to PAP RFD at 20 seconds, 4.5 minutes, and 20 minutes (torque: R = 0.26 and 0.34, p ≤ 0.05; RFD: R = 0.65, 0.52, and 0.41). The gastrocnemius did not significantly contribute to PAP. Both muscle and neural factors play a significant role in PAP, and neural factors may play a more prominent role in RFD potentiation than torque potentiation.

Examination of a Multi-ingredient Preworkout Supplement on Total Volume of Resistance Exercise and Subsequent Strength and Power Performance.

Bergstrom, HC, Byrd, MT, Wallace, BJ, and Clasey, JL. Examination of a multi-ingredient preworkout supplement on total volume of resistance exercise and subsequent strength and power performance. J Strength Cond Res 32(6): 1479-1490, 2018-This study examined the acute effects of a multi-ingredient preworkout supplement on (a) total-, lower-, and upper-body volume of resistance exercise and (b) the subsequent lower-body strength (isokinetic leg extension and flexion), lower-body power (vertical jump [VJ] height), upper-body power (bench throw velocity [BTv]), and cycle ergometry performance (critical power and anaerobic work capacity). Twelve men completed baseline strength and power measures before 2 experimental visits, supplement (SUP) and placebo (PL). The experimental visits involved a fatiguing cycling protocol 30 minutes after ingestion of the SUP or PL and 15 minutes before the beginning of the resistance exercise protocol, which consisted of 4 upper-body and 4 lower-body resistance exercises performed for 4 sets to failure at 75% 1 repetition maximum. The exercise volume for the total, lower, and upper body was assessed. The VJ height and BTv were measured immediately after the resistance exercise. Postexercise isokinetic leg extension and flexion strength was measured 15 minutes after the completion of a second cycling protocol. There was a 9% increase in the total-body volume of exercise and a 14% increase in lower-body volume of exercise for the SUP compared with the PL, with no effect on exercise volume for the upper body between the SUP and PL. The increased lower-body volume for the SUP did not result in greater lower-body strength and power performance decrements after exhaustive exercise, compared with the PL. These findings suggested the potential for the SUP to increase resistance exercise volume, primarily related to an increased lower-body volume of exercise.

Optimum Drop Jump Height in Division III Athletes: Under 75% of Vertical Jump Height.

Our purpose was to evaluate the vertical ground reaction force, impulse, moments and powers of hip, knee and ankle joints, contact time, and jump height when performing a drop jump from different drop heights based on the percentage of a performer's maximum vertical jump height (MVJH). Fifteen male Division III athletes participated voluntarily. Eleven synchronized cameras and two force platforms were used to collect data. One-way repeated-measures analysis of variance tests were used to examine the differences between drop heights. The maximum hip, knee and ankle power absorption during 125%MVJH and 150%MVJH were greater than those during 75%MVJH. The impulse during landing at 100%MVJH, 125%MVJH and 150%MVJH were greater than 75%MVJH. The vertical ground reaction force during 150%MVJH was greater than 50%MVJH, 75%MVJH and 100%MVJH. Drop height below 75%MVJH had the most merits for increasing joint power output while having a lower impact force, impulse and joint power absorption. Drop height of 150%MVJH may not be desirable as a high-intensity stimulus due to the much greater impact force, increasing the risk of injury, without increasing jump height performance.

Soil Aggregate Dynamics and Plant Community Response after Biosolids Application in a Semiarid Grassland.

Biosolids may improve the ecological function of degraded semiarid grasslands, but an understanding of the plant community is essential. An experiment was established in 2001 to determine the effects of a single surface application of biosolids on soil aggregate stability and the composition of the plant community in a semiarid grassland in British Columbia, Canada. Four treatments were evaluated: (i) surface biosolids application at 20 (Bio-20) and (ii) 60 Mg ha (Bio-60), (iii) mineral fertilizer, and (iv) a control. All treatments were replicated in four blocks. Soil was sampled during the spring (May), summer (June-July), and fall (October) in 2005, 2006, and 2009; the plant community was assessed in 2009. The greatest increases in size of stable aggregates relative to the control were in the spring and summer, which coincided with a 1.6- to 2.1-fold increase in the spring concentration of N within stable aggregates when biosolids were applied at 20 and 60 Mg ha , respectively. Nitrogen concentrations from the Bio-60 treatment were not different from the control, but the Bio-20 treatment had 42% greater N than all other treatments during summer. Biosolids application in this ecosystem did not increase perennial forage grass species relative to the control, and when biosolids were applied at a rate of 60 Mg ha there was a 75% reduction in the perennial forage plant species. The application of biosolids to native grasslands in semiarid environments should be done cautiously, especially when winter annual plant species (e.g., cheatgrass [ L.]) are present before application.

Differing effects of biosolids on native plants in grasslands of southern british columbia.

The objective of this study was to determine if application of biosolids is beneficial for restoring semiarid grasslands. The effects of a one-time surface application of biosolids at a rate of 20 Mg ha on individual plant species and plant community composition were examined at three degraded semiarid grassland sites located in the southern interior of British Columbia, Canada. Biosolids application did not result in desirable changes in plant species composition at the two drier sites (with annual precipitation <400 mm) yet led to overall positive plant species changes at the least dry site (with annual precipitation of 400 mm). An important late-seral species of semiarid grasslands, bluebunch wheatgrass [ (Pursh) Á. Löve], did not respond or decreased at the two drier sites but increased at the least dry site. Exotic invasive plant species increased on all sites. The total vegetative cover of native perennial grasses increased from 41 to 99% at the least dry site, with important decreases in low-growing, early-seral forb species. Although cheatgrass ( L.) was not significantly increased by the biosolids treatment, the dominance of cheatgrass and lower growing season precipitation at the two drier sites were likely key reasons for the poor biosolids restoration success at these sites. Despite some concerns, there is potential to use biosolids to restore grassland plant communities successfully within 4 or 5 yr on more mesic grassland sites; however, biosolids use on drier sites where exotic invasives are present cannot be recommended.

Examining pretrial publicity in a shadow jury paradigm: issues of slant, quantity, persistence and generalizability.

The purpose of this study was to examine the influence of pretrial publicity (PTP) on mock juror decision making. Specifically, we examined the influence of quantity and slant of the PTP (proprosecution vs. prodefense), the persistence of PTP effects over time, and whether the PTP effects demonstrated in research laboratories would also occur in more naturalistic settings (generalizability). Using a shadow jury paradigm we examined these effects using a real trial as stimulus. Mock jurors included 115 jury-eligible community members who were naturally exposed to PTP in the venue in which the actual case occurred and 156 who were experimentally exposed. We found mock jurors were significantly influenced by both the slant and quantity of the PTP to which they were exposed, such that those exposed to proprosecution or prodefense PTP tended to render decision in support of the party favored in the PTP, and those exposed to greater quantities of PTP tended to be more biased. Additionally, PTP effects persisted throughout the course of the trial and continued to influence judgments in face of trial evidence and arguments. A finding of no significant difference in the effect of exposure slant between the naturally exposed and experimentally exposed samples provides support for the external validity of laboratory studies examining PTP effects. This research helps address some of the concerns raised by courts with regard to the durability of PTP effects and the application of laboratory findings to real world settings.

Bridging the gap from medical to psychological safety assessment: consensus study in a digital mental health context.

BACKGROUND: Digital Mental Health Interventions (DMHIs) that meet the definition of a medical device are regulated by the Medicines and Healthcare products Regulatory Agency (MHRA) in the UK. The MHRA uses procedures that were originally developed for pharmaceuticals to assess the safety of DMHIs. There is recognition that this may not be ideal, as is evident by an ongoing consultation for reform led by the MHRA and the National Institute for Health and Care Excellence. AIMS: The aim of this study was to generate an experts' consensus on how the medical regulatory method used for assessing safety could best be adapted for DMHIs. METHOD: An online Delphi study containing three rounds was conducted with an international panel of 20 experts with experience/knowledge in the field of UK digital mental health. RESULTS: Sixty-four items were generated, of which 41 achieved consensus (64%). Consensus emerged around ten recommendations, falling into five main themes: Enhancing the quality of adverse events data in DMHIs; Re-defining serious adverse events for DMHIs; Reassessing short-term symptom deterioration in psychological interventions as a therapeutic risk; Maximising the benefit of the Yellow Card Scheme; and Developing a harmonised approach for assessing the safety of psychological interventions in general. CONCLUSION: The implementation of the recommendations provided by this consensus could improve the assessment of safety of DMHIs, making them more effective in detecting and mitigating risk.

Analysis of poor outcomes after laparoscopic adjustable gastric banding.

BACKGROUND: Recent studies document excess weight loss (EWL) of more than 50% with the laparoscopic adjustable gastric band (LGB). This study reviews the LGB experience at an urban academic center in terms of complications, reoperative rates, and comorbidities. METHODS: In this study, 144 consecutive patients undergoing LGB were prospectively reviewed. Data were collected including weight, body mass index (BMI), excess weight loss (EWL), comorbidities, and complications. Demographics were analyzed using a t-test. Linear regression was used to analyze the relationship of BMI, race, and age to EWL at 12 months. RESULTS: The study participants were 130 women with a mean age of 43 ± 11 years, a mean weight of 127.1 kg ± 20.5 kg, and a mean BMI of 45.6 ± 6.1. The mean follow-up period was 16 months. The mean EWL was 20% ± 14% at 6 months (n = 118), 26% ± 16% at 12 months (n = 106), 30% ± 20% at 18 months (n = 68), and 34% ± 23% at 24 months (n = 43). Patients with a BMI higher than 50 kg/m(2) had a lower EWL at 12 months than patients with a BMI lower than 50 kg/m(2) (P = 0.00005). The mean EWL at 12 months was significantly less for African Americans than for Caucasians (P = 0.0046; 95% confidence interval [CI] 3-15%). Patients older than 50 years had a lower EWL, but the difference was not statistically significant (P = 0.07). Complete and partial resolution of comorbidities occurred for 10% and 4% of the patients, respectively. Removal of the band with revision to a sleeve gastrectomy for inadequate EWL was required for 14 patients (11.5%). Complications occurred for 8% of the patients (n = 15) including port flipping, stoma obstruction, tube disconnection, port infections, dysphagia, and band slippage. Overall, 16.7% of the patients (n = 24) required reoperation. CONCLUSION: After LGB, a majority of the patients failed to achieve a 50% EWL, and 16.7% required reoperation. Laparoscopic adjustable gastric banding may not be the optimal bariatric procedure for patients older than 50 years, patients with a BMI higher than 50 kg/m(2), or African Americans.

Enfuvirtide injection site reactions: a clinical and histopathological appraisal.

BACKGROUND/OBJECTIVES: Enfuvirtide was the first of a new class of antiretroviral agents termed 'fusion inhibitors' used for the treatment of HIV-1 infection. Enfuvirtide is administered subcutaneously and injection site reactions (ISR) are commonplace (98%). The aim of this study was to analyse in detail the histopathological changes associated with striking ISR seen in four patients. METHODS: Biopsies were obtained at various times post-injection and were reviewed histologically. The changes in epidermal, dermal and subcutaneous connective tissue and the presence and nature of the inflammatory cellular infiltrate were noted. An immunohistochemical assessment was undertaken. RESULTS: All biopsy specimens demonstrated striking changes in the dermal connective tissue. Alteration in collagen was the most prominent feature and resembled a morphoea/scleroderma-like process. These changes persisted well beyond cessation of enfuvirtide (>1 year). The relative populations of dermal dendritic cells (DDC) (types 1 (Factor XIIIa) and 2 (CD34+)) were analysed and a reciprocal relationship between DDC subpopulations was observed akin to that observed in other sclerosing and fibrosing conditions. CONCLUSION: This study details histopathological changes associated with enfuvirtide ISR. We postulate that changes in DDC populations may contribute to the pathogenesis of the sclerotic process observed with enfuvirtide ISR.

Student perceptions of low-tech active learning and mastery of introductory biomechanics concepts.

This study documented student perceptions of five low-tech active learning exercises, their epistemology of learning, and examined the association between these variables and mastery of biomechanics concepts. Students (N = 152) in four introductory biomechanics courses at two universities completed the Biomechanics Concept Inventory (BCI) at the beginning and the end of the course. An additional 10-question survey was used at the end of the course to determine student perceptions of the active learning exercises and their epistemology of learning. Student learning of biomechanical concepts improved over levels reported in previous studies of traditional lecture instruction, but not as much as seen in other studies of active learning pedagogy in biomechanics and physics. Student perceptions of active learning were positive, particularly in individual rather than group exercises. A minority (12-16%) of these students had negative perceptions of group-based active learning exercises. Student perception of epistemology of learning was primarily constructivist; however, there was no evidence of these perceptions had associations with learning biomechanical concepts. Biomechanics instructors planning to use low-tech active learning exercises should communicate their philosophy of learning, expectations for the course, and progressively implement individual-based and group-based active learning experiences early in the course.

Quantification of vertical ground reaction forces of popular bilateral plyometric exercises.

The purpose of this study was to quantify the vertical ground reaction forces (VGRFs) developed during the performance of popular bilateral plyometric movements. Fourteen power-oriented track and field men of collegiate and national level randomly performed 3 trials of 9 different bilateral plyometric exercises in a single testing session. Three depth drop (DD) and 3 depth jump (DJ) conditions from 30, 60, and 90 cm heights (DD30, DD60, and DD90 and DJ30, DJ60, and DJ90) were tested, in addition to vertical jump (VJ), standing long jump (SLJ), and 2 consecutive jump (2CJ) conditions. Peak impact VGRFs were normalized to body weight. Additionally, all conditions were compared against the VJ in an intensity index. The SLJ condition resulted in a significantly higher peak VGRF than the 2CJ condition (p < or = 0.05). 90DD, 90DJ, 60DD, and SLJ had a significantly greater peak VGRF (5.39, 4.93, 4.30, and 4.22 times body weight, respectively) than the VJ condition (3.34 times body weight). The 30DJ condition had an insignificantly smaller peak VGRF (2.78 times body weight) when compared with the VJ. Practitioners may use these findings to more effectively progress athletes in these movements based on their intensities.

The effect of course format on student learning in introductory biomechanics courses that utilise low-tech active learning exercises.

Low-tech active learning (AL) exercises in face-to-face (F2F) undergraduate biomechanics courses improve student learning vs. lecture alone. This study compared learning of biomechanics concepts with AL implemented in two course formats (hybrid: HB vs. F2F). Additional aims were to investigate if student perceptions of learning epistemology and learning factors were related to course format. Students (n = 110) in four introductory biomechanics courses (two F2F, two HB) completed the 24-question Biomechanics Concept Inventory (BCI) at the beginning and the end of the course to determine their learning of biomechanical concepts. An additional eight questions were given with the post-test to determine student perceptions of the AL exercises and their epistemology of learning. Learning in the HB format was equivalent to the F2F course format when both implement AL in these students. Student perceptions of AL were generally positive and learning scores consistent with previous research on AL in biomechanics. There were mixed results of the effect of course format with one significant difference of three ratings of the nature of learning biomechanics and one significant difference of four ratings of AL by students. These results should be replicated and potential interactions with student perceptions and characteristics explored.

The influence of hip rotations on muscle activity during unilateral weight-bearing exercises in individuals with and without genu varum: A cross-sectional study.

OBJECTIVES: Hip rotation can influence gluteus medius (Gmed) muscle activity in individuals with genu varum (GV). This study examined different hip rotation positions on muscle activity during unilateral weight-bearing exercises in individuals with and without GV. DESIGN: Cross-sectional. SETTING: Outpatient physical therapy center. PARTICIPANTS: Forty-six physically active males, divided between control (n = 23) and GV groups (n = 23). MAIN OUTCOME MEASURES: Surface electromyography measured Gmed and tensor fascia latae (TFL) activity during pelvic drop (PD) and wall press (WP) exercises with different hip rotations. RESULTS: In the control group, WP with external rotation produced greater Gmed/TFL activity compared to other rotation positions. No difference was found for Gmed/TFL with PD in controls. In the GV group, Gmed activity increased with internal rotation in PD and WP; TFL activity increased only with external rotation in WP. Internal hip rotation produced higher Gmed/TFL activity than other positions in the GV group during both exercises. Greater Gmed/TFL activity was observed in the control group than GV during PD with external rotation, and WP with neutral positioning and external rotation. CONCLUSIONS: WP with external rotation in controls, and PD and WP with internal hip rotation in subjects with GV, are effective exercises to optimize GMed/TFL activity.

Creating and testing a GCP game in an asynchronous course environment: The game and future plans.

INTRODUCTION: The National Institute of Health has mandated good clinical practice (GCP) training for all clinical research investigators and professionals. We developed a GCP game using the Kaizen-Education platform. The GCP Kaizen game was designed to help clinical research professionals immerse themselves into applying International Conference on Harmonization GCP (R2) guidelines in the clinical research setting through case-based questions. METHODS: Students were invited to participate in the GCP Kaizen game as part of their 100% online academic Masters during the Spring 2019 semester. The structure of the game consisted of 75 original multiple choice and 25 repeated questions stemming from fictitious vignettes that were distributed across 10 weeks. Each question presented a teachable rationale after the answers were submitted. At the end of the game, a satisfaction survey was issued to collect player satisfaction data on the game platform, content, experience as well as perceptions of GCP learning and future GCP concept application. RESULTS: There were 71 total players who participated and answered at least one question. Of those, 53 (75%) answered all 100 questions. The game had a high Cronbach's alpha, and item analyses provided information on question quality, thus assisting us in future quality edits before re-testing and wider dissemination. CONCLUSIONS: The GCP Kaizen game provides an alternative method for mandated GCP training using principles of gamification. It proved to be a reliable and an effective educational method with high player satisfaction.

Biosolids increase soil aggregation and protection of soil carbon five years after application on a crested wheatgrass pasture.

Biosolids application to rangelands and pastures recycles nutrients and organic matter back to soils. The effects of biosolids (20 and 60 dry Mg ha(-)(1)) and N+P fertilizer on soil aggregate stability, bulk density, aeration porosity, and total C and N of stable aggregates were evaluated 4 and 5 yr after surface application to a crested wheatgrass [Agropyron cristatum (L.) Gaertn.] pasture in the southern interior of British Columbia (BC). The experiment was established in 2001 in a randomized complete block design with four replications. The 60 Mg ha(-1) biosolids treatment (Bio 60) had a greater aggregate mean weight diameter (MWD) and proportion of water-stable soil aggregates > 1 mm relative to the control and fertilizer treatments. Temporal variation in aggregate stability was attributed to seasonal variations in soil water content. Surface application of 60 Mg ha(-1) of biosolids increased C concentrations within water-stable aggregates relative to the control from 29 to 104, 24 to 79, and 12 to 38 g kg(-1) for the 2 to 6, 1 to 2, and 0.25 to 1 mm size fractions, respectively. The concentration of N within aggregates increased in similar proportions to C. Neither soil bulk density, nor aeration porosity were affected by biosolids application. Increased aggregation and the accumulation of soil C within aggregates following biosolids application creates a potential for better soil C storage, soil water retention, nutrient availability, and ultimately the overall health of semiarid perennial pastures.