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1.
Cell ; 153(5): 1012-24, 2013 May 23.
Artículo en Inglés | MEDLINE | ID: mdl-23706739

RESUMEN

Histone acetylation plays critical roles in chromatin remodeling, DNA repair, and epigenetic regulation of gene expression, but the underlying mechanisms are unclear. Proteasomes usually catalyze ATP- and polyubiquitin-dependent proteolysis. Here, we show that the proteasomes containing the activator PA200 catalyze the polyubiquitin-independent degradation of histones. Most proteasomes in mammalian testes ("spermatoproteasomes") contain a spermatid/sperm-specific α subunit α4 s/PSMA8 and/or the catalytic ß subunits of immunoproteasomes in addition to PA200. Deletion of PA200 in mice abolishes acetylation-dependent degradation of somatic core histones during DNA double-strand breaks and delays core histone disappearance in elongated spermatids. Purified PA200 greatly promotes ATP-independent proteasomal degradation of the acetylated core histones, but not polyubiquitinated proteins. Furthermore, acetylation on histones is required for their binding to the bromodomain-like regions in PA200 and its yeast ortholog, Blm10. Thus, PA200/Blm10 specifically targets the core histones for acetylation-mediated degradation by proteasomes, providing mechanisms by which acetylation regulates histone degradation, DNA repair, and spermatogenesis.


Asunto(s)
Reparación del ADN , Histonas/metabolismo , Proteínas Nucleares/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Espermatogénesis , Testículo/metabolismo , Acetilación , Secuencia de Aminoácidos , Animales , Roturas del ADN de Doble Cadena , Humanos , Masculino , Ratones , Datos de Secuencia Molecular , Proteínas Nucleares/química , Estructura Terciaria de Proteína , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Alineación de Secuencia
2.
Proc Natl Acad Sci U S A ; 120(29): e2301002120, 2023 07 18.
Artículo en Inglés | MEDLINE | ID: mdl-37428930

RESUMEN

Autophagy is a major means for the elimination of protein inclusions in neurons in neurodegenerative diseases such as Parkinson's disease (PD). Yet, the mechanism of autophagy in the other brain cell type, glia, is less well characterized and remains largely unknown. Here, we present evidence that the PD risk factor, Cyclin-G-associated kinase (GAK)/Drosophila homolog Auxilin (dAux), is a component in glial autophagy. The lack of GAK/dAux increases the autophagosome number and size in adult fly glia and mouse microglia, and generally up-regulates levels of components in the initiation and PI3K class III complexes. GAK/dAux interacts with the master initiation regulator UNC-51like autophagy activating kinase 1/Atg1 via its uncoating domain and regulates the trafficking of Atg1 and Atg9 to autophagosomes, hence controlling the onset of glial autophagy. On the other hand, lack of GAK/dAux impairs the autophagic flux and blocks substrate degradation, suggesting that GAK/dAux might play additional roles. Importantly, dAux contributes to PD-like symptoms including dopaminergic neurodegeneration and locomotor function in flies. Our findings identify an autophagy factor in glia; considering the pivotal role of glia under pathological conditions, targeting glial autophagy is potentially a therapeutic strategy for PD.


Asunto(s)
Proteínas de Drosophila , Enfermedad de Parkinson , Animales , Ratones , Drosophila/metabolismo , Auxilinas/metabolismo , Homólogo de la Proteína 1 Relacionada con la Autofagia/genética , Homólogo de la Proteína 1 Relacionada con la Autofagia/metabolismo , Autofagia , Ciclinas/metabolismo , Neuroglía/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Proteínas Relacionadas con la Autofagia/metabolismo , Proteínas de la Membrana/metabolismo
3.
J Pathol ; 262(3): 320-333, 2024 03.
Artículo en Inglés | MEDLINE | ID: mdl-38108121

RESUMEN

Bone morphogenetic protein (BMP)-Smad1/5/8 signaling plays a crucial regulatory role in lung development and adult lung homeostasis. However, it remains elusive whether BMP-Smad1/5/8 signaling is involved in the pathogenesis of emphysema. In this study, we downregulated BMP-Smad1/5/8 signaling by overexpressing its antagonist Noggin in adult mouse alveolar type II epithelial cells (AT2s), resulting in an emphysematous phenotype mimicking the typical pathological features of human emphysema, including distal airspace enlargement, pulmonary inflammation, extracellular matrix remodeling, and impaired lung function. Dysregulation of BMP-Smad1/5/8 signaling in AT2s leads to inflammatory destruction dominated by macrophage infiltration, associated with reduced secretion of surfactant proteins and inhibition of AT2 proliferation and differentiation. Reactivation of BMP-Smad1/5/8 signaling by genetics or chemotherapy significantly attenuated the morphology and pathophysiology of emphysema and improved the lung function in Noggin-overexpressing lungs. We also found that BMP-Smad1/5/8 signaling was downregulated in cigarette smoke-induced emphysema, and that enhancing its activity in AT2s prevented or even reversed emphysema in the mouse model. Our data suggest that BMP-Smad1/5/8 signaling, located at the top of the signaling cascade that regulates lung homeostasis, represents a key molecular regulator of alveolar stem cell secretory and regenerative function, and could serve as a potential target for future prevention and treatment of pulmonary emphysema. © 2023 The Pathological Society of Great Britain and Ireland.


Asunto(s)
Enfisema , Enfisema Pulmonar , Transducción de Señal , Animales , Humanos , Ratones , Células Epiteliales Alveolares/metabolismo , Enfisema/metabolismo , Pulmón/metabolismo , Enfisema Pulmonar/genética , Transducción de Señal/fisiología , Proteína Smad1/genética , Proteína Smad1/metabolismo
4.
Traffic ; 23(10): 506-520, 2022 10.
Artículo en Inglés | MEDLINE | ID: mdl-36053864

RESUMEN

Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial and sporadic Parkinson's disease. A plethora of evidence has indicated a role for LRRK2 in endolysosomal trafficking in neurons, while LRRK2 function in glia, although highly expressed, remains largely unknown. Here, we present evidence that LRRK2/dLRRK mediates a lysosomal pathway that contributes to glial cell death and the survival of dopaminergic (DA) neurons. LRRK2/dLRRK knockdown in the immortalized microglia or flies results in enlarged and swelling lysosomes fewer in number. These lysosomes are less mobile, wrongly acidified, exhibit defective membrane permeability and reduced activity of the lysosome hydrolase cathepsin B. In addition, LRRK2/dLRRK depletion causes glial apoptosis, DA neurodegeneration, and locomotor deficits in an age-dependent manner. Taken together, these findings demonstrate a functional role of LRRK2/dLRRK in regulating the glial lysosomal pathway; deficits in lysosomal biogenesis and function linking to glial apoptosis potentially underlie the mechanism of DA neurodegeneration, providing insights on LRRK2/dLRRK function in normal and pathological brains.


Asunto(s)
Catepsina B , Neuronas Dopaminérgicas , Catepsina B/genética , Catepsina B/metabolismo , Muerte Celular , Neuronas Dopaminérgicas/metabolismo , Leucina/genética , Leucina/metabolismo , Proteína 2 Quinasa Serina-Treonina Rica en Repeticiones de Leucina/genética , Proteína 2 Quinasa Serina-Treonina Rica en Repeticiones de Leucina/metabolismo , Lisosomas/metabolismo , Mutación , Neuroglía/metabolismo
5.
Genome Res ; 31(1): 13-26, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-33328167

RESUMEN

Long noncoding RNAs (lncRNAs) have emerged as diverse functional regulators involved in mammalian development; however, large-scale functional investigation of lncRNAs in mammalian spermatogenesis in vivo is lacking. Here, we delineated the global lncRNA expression landscape in mouse spermatogenesis and identified 968 germ cell signature lncRNAs. By combining bioinformatics and functional screening, we identified three functional lncRNAs (Gm4665, 1700027A15Rik, and 1700052I22Rik) that directly influence spermatogenesis in vivo. Knocking down Gm4665 hampered the development of round spermatids into elongating spermatids and disrupted key spermatogenic gene expression. Mechanistically, lncRNA Gm4665 localized in the nucleus of round spermatids and occupied the genomic regulatory region of important spermatogenic genes including Ip6k1 and Akap3 These findings provide a valuable resource and framework for future functional analysis of lncRNAs in spermatogenesis and their potential roles in other biological processes.


Asunto(s)
Espermatogénesis , Animales , Perfilación de la Expresión Génica , Masculino , Ratones , ARN Largo no Codificante/genética , Espermátides , Espermatogénesis/genética , Transcriptoma
6.
Proc Natl Acad Sci U S A ; 118(23)2021 06 08.
Artículo en Inglés | MEDLINE | ID: mdl-34078666

RESUMEN

Intrinsic mechanisms such as temporal series of transcription factors orchestrate neurogenesis from a limited number of neural progenitors in the brain. Extrinsic regulations, however, remain largely unexplored. Here we describe a two-step glia-derived signal that regulates neurogenesis in the Drosophila mushroom body (MB). In a temporal manner, glial-specific ubiquitin ligase dSmurf activates non-cell-autonomous Hedgehog signaling propagation by targeting the receptor Patched to suppress and promote the exit of MB neuroblast (NB) proliferation, thereby specifying the correct α/ß cell number without affecting differentiation. Independent of NB proliferation, dSmurf also stabilizes the expression of the cell-adhesion molecule Fasciclin II (FasII) via its WW domains and regulates FasII homophilic interaction between glia and MB axons to refine α/ß-lobe integrity. Our findings provide insights into how extrinsic glia-to-neuron communication coordinates with NB proliferation capacity to regulate MB neurogenesis; glial proteostasis is likely a generalized mechanism in orchestrating neurogenesis.


Asunto(s)
Comunicación Celular , Proliferación Celular , Cuerpos Pedunculados/embriología , Neurogénesis , Neuroglía/metabolismo , Animales , Proteínas de Drosophila/metabolismo , Drosophila melanogaster
7.
Environ Monit Assess ; 195(4): 455, 2023 Mar 09.
Artículo en Inglés | MEDLINE | ID: mdl-36892619

RESUMEN

Inter-basin water transfer (IBWT) projects have been widely constructed to alleviate the pressure on water resources in water shortage basins. However, the ecological effects of IBWT projects have often been ignored. Based on the Soil and Water Assessment Tool (SWAT) model and a constructed total ecosystem services (TES) index, the impacts of IBWT projects on recipient basin ecosystem services were analyzed in this study. The results showed that the TES index was relatively stable from 2010 to 2020, but in the wet season it was 1.36 times that of the other months with high water yield and nutrient loads. Spatially, areas with high index values were mainly distributed in the sub-basins around the reservoirs. The IBWT projects had positive impacts on ecosystem services, and the TES index with IBWT projects was 5.98% higher than that without projects. Water yield and total nitrogen were the two most affected indexes, with increased of 5.65% and 5.41%, respectively, under the impacts of IBWT projects. Seasonally, the change rates of the TES index were less than 3% while the change rates of water yield and nitrogen load peaked at 8.23% and 53.42%, respectively, in March, owing to the large amount of water released from the reservoirs. Areas affected by the three evaluated IBWT projects accounted for 61%, 18%, and 11% of the watershed, respectively. Under the impact of each project, the TES index generally increased, whereas the impact decreased as the distance from the inflow location increased. Intense changes in ecosystem services occurred in sub-basin 23, the sub-basin closest to an IBWT project, with water yield, water flow, and local climate regulation increasing the largest.


Asunto(s)
Ecosistema , Ríos , Suelo , Agua , Monitoreo del Ambiente , Nitrógeno/análisis
8.
J Biol Chem ; 296: 100512, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33676893

RESUMEN

Smad2 and Smad3 (Smad2/3) are structurally similar proteins that primarily mediate the transforming growth factor-ß (TGF-ß) signaling responsible for driving cell proliferation, differentiation, and migration. The dynamics of the Smad2/3 phosphorylation provide the key mechanism for regulating the TGF-ß signaling pathway, but the details surrounding this phosphorylation remain unclear. Here, using in vitro kinase assay coupled with mass spectrometry, we identified for the first time that nemo-like kinase (NLK) regulates TGF-ß signaling via modulation of Smad2/3 phosphorylation in the linker region. TGF-ß-mediated transcriptional and cellular responses are suppressed by NLK overexpression, whereas NLK depletion exerts opposite effects. Specifically, we discovered that NLK associates with Smad3 and phosphorylates the designated serine residues located in the linker region of Smad2 and Smad3, which inhibits phosphorylation at the C terminus, thereby decreasing the duration of TGF-ß signaling. Overall, this work demonstrates that phosphorylation on the linker region of Smad2/3 by NLK counteracts the canonical phosphorylation in response to TGF-ß signals, thus providing new insight into the mechanisms governing TGF-ß signaling transduction.


Asunto(s)
Proteínas Serina-Treonina Quinasas/farmacología , Proteína Smad2/metabolismo , Proteína smad3/metabolismo , Factor de Crecimiento Transformador beta/metabolismo , Diferenciación Celular , Movimiento Celular , Proliferación Celular , Humanos , Fosforilación , Transducción de Señal , Proteína Smad2/genética , Proteína smad3/genética , Factor de Crecimiento Transformador beta/genética
9.
Biol Reprod ; 107(5): 1331-1344, 2022 11 14.
Artículo en Inglés | MEDLINE | ID: mdl-35980806

RESUMEN

Spermatogenesis is sustained by homeostatic balance between the self-renewal and differentiation of spermatogonial stem cells, which is dependent on the strict regulation of transcription factor and chromatin modulator gene expression. Chromodomain helicase DNA-binding protein 4 is highly expressed in spermatogonial stem cells but roles in mouse spermatogenesis are not fully understood. Here, we report that the germ-cell-specific deletion of chromodomain helicase DNA-binding protein 4 resulted in complete infertility in male mice, with rapid loss of spermatogonial stem cells and excessive germ cell apoptosis. Chromodomain helicase DNA-binding protein 4-knockdown in cultured spermatogonial stem cells also promoted the expression of apoptosis-related genes and thereby activated the tumor necrosis factor signaling pathway. Mechanistically, chromodomain helicase DNA-binding protein 4 occupies the genomic regulatory region of key apoptosis-related genes, including Jun and Nfkb1. Together, our findings reveal the determinant role of chromodomain helicase DNA-binding protein 4 in spermatogonial stem cells survival in vivo, which will offer insight into the pathogenesis of male sterility and potential novel therapeutic targets.


Asunto(s)
Células Madre Germinales Adultas , Animales , Masculino , Ratones , Células Madre Germinales Adultas/metabolismo , Diferenciación Celular/genética , Diferenciación Celular/fisiología , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Espermatogénesis/genética , Espermatogonias/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , ADN Helicasas/genética , ADN Helicasas/metabolismo
10.
Int J Mol Sci ; 23(23)2022 Nov 25.
Artículo en Inglés | MEDLINE | ID: mdl-36499080

RESUMEN

Parkinson's disease (PD) is a progressive neurodegenerative disorder. The classical behavioral defects of PD patients involve motor symptoms such as bradykinesia, tremor, and rigidity, as well as non-motor symptoms such as anosmia, depression, and cognitive impairment. Pathologically, the progressive loss of dopaminergic (DA) neurons in the substantia nigra (SN) and the accumulation of α-synuclein (α-syn)-composed Lewy bodies (LBs) and Lewy neurites (LNs) are key hallmarks. Glia are more than mere bystanders that simply support neurons, they actively contribute to almost every aspect of neuronal development and function; glial dysregulation has been implicated in a series of neurodegenerative diseases including PD. Importantly, amounting evidence has added glial activation and neuroinflammation as new features of PD onset and progression. Thus, gaining a better understanding of glia, especially neuron-glia crosstalk, will not only provide insight into brain physiology events but also advance our knowledge of PD pathologies. This review addresses the current understanding of α-syn pathogenesis in PD, with a focus on neuron-glia crosstalk. Particularly, the transmission of α-syn between neurons and glia, α-syn-induced glial activation, and feedbacks of glial activation on DA neuron degeneration are thoroughly discussed. In addition, α-syn aggregation, iron deposition, and glial activation in regulating DA neuron ferroptosis in PD are covered. Lastly, we summarize the preclinical and clinical therapies, especially targeting glia, in PD treatments.


Asunto(s)
Enfermedad de Parkinson , alfa-Sinucleína , Humanos , alfa-Sinucleína/metabolismo , Enfermedad de Parkinson/patología , Cuerpos de Lewy/metabolismo , Sustancia Negra/metabolismo , Neuronas Dopaminérgicas/metabolismo , Degeneración Nerviosa/patología
11.
J Environ Manage ; 305: 114382, 2022 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-34973559

RESUMEN

The spatial distributions, sources, and source-specific risk apportionments of 26 antibiotics (5 categories) in the Fenhe River basin were determined based on sample data. The results showed that antibiotics were widely distributed in the surface water. There were significant differences between the different types of antibiotics, and the highest mean concentration was that of the sulfonamide category (33.74 ng/L), accounting for 36% of the total antibiotic concentration. Spatially, all antibiotics were mainly detected in the middle and downstream areas. The ecological risk assessment results showed that the significant risk rate of antibiotics accounted for 70% and was mainly distributed in the downstream area; however, the risks differed between the 5 categories. Quinolone antibiotics exhibited the highest significant risk rate, reaching 100%. The ecological risk associated with sulfamethoxazole was the highest among all detected antibiotics. The following five main factors influenced the antibiotic concentrations: aquaculture, pharmaceutical wastewater, livestock discharges, domestic sewage, and sewage treatment plants. Among these, pharmaceutical wastewater sources contributed the most (35%) to the total antibiotic concentration, and were distributed throughout the river. Although livestock discharges were not the main reason for the high level of ecological risk, these discharges were highest at certain sites in the midstream region. Different pollution sources posed different levels of ecological risk to the Fenhe River basin, the highest of which was pharmaceutical wastewater with a significant risk rate of 58%.


Asunto(s)
Monitoreo del Ambiente , Contaminantes Químicos del Agua , Antibacterianos/análisis , China , Medición de Riesgo , Ríos , Contaminantes Químicos del Agua/análisis
12.
Yi Chuan ; 44(12): 1103-1116, 2022 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-36927556

RESUMEN

Spermatogonial stem cells (SSCs) are germ cells (GCs) with long-term self-renewal and differentiation potential in testis, namely tissue stem cells located on the basement membrane, whose self-renewal and differentiation are regulated by the surrounding microenvironment. In recent years, the research of SSCs has made a series of important progress, which brings the hope for the clinical treatment of some male infertility patients. Among them, the microenvironment is particularly important in regulating SSCs. The microenvironment is responsible for integrating the effects of different types of cell components, extracellular matrix, extracellular regulatory molecules and hormones on SSCs, thus regulating the fate of SSCs. The research on SSCs microenvironment has gradually become one of the main contents of stem cell research. In this review, we mainly summarize the cell composition, regulatory factors and characteristics of mouse SSCs microenvironment, thereby providing background information for in-depth study on the structure and function of SSCs microenvironment, and opportunity to find more abundant cell phenotypes and microenvironmental factors through multiple research models in the future.


Asunto(s)
Infertilidad Masculina , Nicho de Células Madre , Humanos , Masculino , Animales , Ratones , Espermatogonias , Testículo , Células Madre
13.
Analyst ; 144(24): 7457-7462, 2019 Dec 21.
Artículo en Inglés | MEDLINE | ID: mdl-31710053

RESUMEN

Glutathione (GSH) plays crucial roles in various physiological and pathological processes. The fluctuation of the GSH level is closely associated with a variety of diseases and cellular functions. Hence, it is important to real-time monitor the fluctuation of GSH in living cells. In this work, we presented a rhodol-hybridized hemicyanine fluorophore (RdH) as a selective, rapid-response, ratiometric, and reversible fluorescent probe for intracellular GSH (t1/2 = 89 ms, Kd = 1.42 mM). The imaging assays in living cells revealed that RdH could be used to real-time monitor GSH dynamics in A549 cells under a laser scanning confocal microscope by ratiometric fluorescence changes.


Asunto(s)
Carbocianinas/química , Colorantes Fluorescentes/química , Glutatión/metabolismo , Imagen Óptica , Xantonas/química , Células A549 , Humanos , Espectrometría de Fluorescencia
14.
Cell Commun Signal ; 16(1): 65, 2018 10 04.
Artículo en Inglés | MEDLINE | ID: mdl-30286765

RESUMEN

BACKGROUND: Our previous work revealed that rhomboid domain-containing protein 1 (RHBDD1) participates in the modulation of cell growth and apoptosis in colorectal cancer cells. This study aimed to investigate the function of RHBDD1 in regulating breast cancer progression and its underlying molecular basis. METHODS: Immunohistochemistry was performed to evaluate RHBDD1 expression in 116 breast cancer tissue and 39 adjacent normal tissue and expression of RHBDD1, phospho-Akt (p-Akt) and cyclin-dependent kinase 2 (CDK2) in the same 84 breast cancer specimens. RHBDD1-knock-out cells were established using breast cancer cell lines. In vitro studies were carried out to estimate the function of RHBDD1 in cell proliferation, migration and invasion. Fluorescence microscopy assay and flow cytometric analysis were used to measure apoptosis and cell cycle regulation. RNA sequencing and western blot analysis were used to investigate the molecular mechanisms of RHBDD1. RESULTS: RHBDD1 was highly up-regulated in breast cancer tissue compared with that in normal tissue and associated with pathological tumor (pT) stage, pathological tumor-node-metastasis (pTNM) stage and estrogen receptor (ER) expression. RHBDD1 up-regulation was associated with poor prognosis in several subtypes of breast cancer. Deletion of RHBDD1 promoted apoptosis and suppressed proliferation, migration and invasion in breast cancer cells. RHBDD1 deletion suppressed Akt activation and decreased CDK2 protein level via proteasome pathway, thus inhibited cell cycle progression and G1/S phase transition. Moreover, the protein level of RHBDD1, p-Akt and CDK2 was significantly positively correlated in breast cancer tissue. CONCLUSIONS: Our study reveals that RHBDD1 promotes breast cancer progression by regulating p-Akt and CDK2 protein levels, and might be a potential biomarker and prognostic indicator for breast cancer patients.


Asunto(s)
Neoplasias de la Mama/patología , Quinasa 2 Dependiente de la Ciclina/metabolismo , Progresión de la Enfermedad , Fosfoproteínas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Serina Endopeptidasas/metabolismo , Apoptosis , Movimiento Celular , Proliferación Celular , Supervivencia Celular , Regulación hacia Abajo , Puntos de Control de la Fase G2 del Ciclo Celular , Eliminación de Gen , Humanos , Puntos de Control de la Fase M del Ciclo Celular , Células MCF-7 , Invasividad Neoplásica , Serina Endopeptidasas/deficiencia , Serina Endopeptidasas/genética
15.
Exp Cell Res ; 359(1): 251-256, 2017 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-28733145

RESUMEN

Cigarette smoking is one of the most important risk factors of atherosclerosis, which can induce endothelial injury. Meanwhile, adipocytes are the main cell type of perivascular adipose tissue (PVAT), the largest endocrine and paracrine organ and direct anatomical connection to adventitia, which may play a key role in the injury of endothelial cells. We used nicotine to induce dysfunctional HUVECs and adipocytes. In addition, we used a novel model to co-culture HUVECs and adipocytes in vitro by the transwell co-culture system to determine the effect of adipocytes on endothelial injury. Cell apoptosis was detected by Annexin V-FITC. Genes and proteins involved in the nuclear factor kappa B (NF-κB) signaling pathway were detected by qRT-PCR and western blot analysis, respectively. We also investigated the nuclear translocation of NF-κB p65 using immunofluorescence staining. Our results showed that nicotine dose-dependently induces the apoptosis of HUVECs and adipocytes and is associated with increased IKKß and NF-κB p65 expression and with IkBα degradation. Meanwhile through the co-culture system, adipocytes promoted the expression of IKKß and NF-κB p65, as well as the translocation of NF-κB p65, and they accelerated the degradation of IkBα, resulting in increased apoptosis of HUVECs compared with that of the single cultured system. In conclusion, adipocytes could promote endothelial injury via the NF-κB pathway. Moreover, the NF-κB pathway plays pivotal roles in nicotine-induced vascular injury.


Asunto(s)
Adipocitos/metabolismo , Adipocitos/patología , Células Endoteliales/patología , FN-kappa B/metabolismo , Nicotina/efectos adversos , Células 3T3-L1 , Adipocitos/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Apoptosis/genética , Diferenciación Celular/efectos de los fármacos , Técnicas de Cocultivo , Células Endoteliales/efectos de los fármacos , Células Endoteliales/metabolismo , Células Endoteliales de la Vena Umbilical Humana/efectos de los fármacos , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Quinasa I-kappa B/metabolismo , Ratones , FN-kappa B/antagonistas & inhibidores , Prolina/análogos & derivados , Prolina/farmacología , Regiones Promotoras Genéticas/genética , Proteolisis/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Tiocarbamatos/farmacología , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/genética
16.
J Cell Biochem ; 118(3): 615-628, 2017 03.
Artículo en Inglés | MEDLINE | ID: mdl-27662601

RESUMEN

1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP), which induces the pathological characteristics of Parkinson's disease in rodents, also specifically targets dopaminergic neurons in zebrafish embryos and larvae. Loganin, a traditional Chinese drug, was reported to regulate immune function and possess anti-inflammatory and anti-shock effects. Here, we investigate the role of loganin in MPTP-induced Parkinson-like abnormalities in zebrafish. MPTP treatment-induced abnormal development, in larvae, such as pericardium edema, increased yolk color, yolk sac edema, and retarded yolk sac resorption, as well as defects in brain development. Loganin could block MPTP-induced defects, with little toxicity to the eggs. Results of whole mount in situ hybridization showed loganin prevented the loss of both dopaminergic neurons and locomotor activity, exhibited by larvae treated with MPTP. In addition, loganin significantly rescued MPTP-induced neurotoxicity on PC12 cells, possibly through the suppression of PI3K/Akt/mTOR axis and JNK signaling pathways. In conclusion, loganin blocks MPTP-induced neurotoxicity and abnormal development in zebrafish. J. Cell. Biochem. 118: 615-628, 2017. © 2016 Wiley Periodicals, Inc.


Asunto(s)
1-Metil-4-fenil-1,2,3,6-Tetrahidropiridina , Iridoides/farmacología , Intoxicación por MPTP/prevención & control , Fármacos Neuroprotectores/farmacología , Pez Cebra/embriología , Animales , MAP Quinasa Quinasa 4/metabolismo , Intoxicación por MPTP/embriología , Células PC12 , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Ratas , Serina-Treonina Quinasas TOR/metabolismo , Proteínas de Pez Cebra/metabolismo
17.
Biochem Biophys Res Commun ; 488(3): 522-527, 2017 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-28522294

RESUMEN

Zebrafish is an excellent model to study the mechanisms underlying successful central nervous system (CNS) regeneration. Previous study shows that activating transcription factor 3 (ATF3) promotes neurite outgrowth and is involved in optic nerve regeneration in zebrafish. Here, we used zebrafish model to investigate the role of ATF3 in regeneration following spinal cord injury (SCI). Quantitative polymerase chain reaction (qPCR) and in situ hybridization revealed that ATF3 mRNA levels increased at 12 h and 6 d following SCI. Double labeled immunofluorescence showed that ATF3 expressed in motoneurons. Treatment of anti-sense ATF3 morpholino (MO) inhibited locomotor recovery and decreased axon regeneration of spinal cord injured zebrafish. Further, inhibition of ATF3 up-regulated the expression of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß). These data suggest that ATF3 could promote locomotor recovery and axon regrowth in zebrafish SCI model possibly by regulating inflammatory response.


Asunto(s)
Factor de Transcripción Activador 3/metabolismo , Regeneración de la Medula Espinal , Pez Cebra/metabolismo , Factor de Transcripción Activador 3/antagonistas & inhibidores , Factor de Transcripción Activador 3/genética , Animales , Perfilación de la Expresión Génica , Interleucina-1beta/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Factor de Necrosis Tumoral alfa/genética
18.
Biochem Biophys Res Commun ; 489(3): 275-280, 2017 07 29.
Artículo en Inglés | MEDLINE | ID: mdl-28559136

RESUMEN

Human spinal cord injury (SCI) usually causes irreversible disability beneath the injured site due to poor neural regeneration. On the contrary, zebrafish show significant regenerative ability after SCI, thus is usually worked as an animal model for studying neuroregeneration. Most of the previous SCI studies focused on the local site of SCI, the supraspinal-derived signals were rarely mentioned. Here we showed that intradiencephalon injection of histamine (HA) inhibited the locomotor recovery in adult zebrafish post-SCI. Immunofluorescence results showed that intradiencephalon HA administration increased the activated microglia 3 days post injury (dpi), promoted the proliferation of radial glial cells at 7 dpi and affected the morphology of radial glial cells at 11 dpi. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) results showed that intradiencephalon HA administration also reduced the expression of neurotrophic factors including brain-derived neurotrophic factor (BDNF) and insulin-like growth factor1 (IGF-1) at the lesion site, however, had no effect on the expression of pro-inflammatory factors such as TNF-alpha and IL-1 beta. Hence, our data suggested that exogenous intradiencephalon HA retarded locomotor recovery in spinal cord injured zebrafish via modulating the repair microenvironment.


Asunto(s)
Histamina/administración & dosificación , Histamina/farmacología , Locomoción/efectos de los fármacos , Traumatismos de la Médula Espinal/tratamiento farmacológico , Traumatismos de la Médula Espinal/fisiopatología , Pez Cebra , Animales , Inyecciones Intraventriculares , Neuroglía/efectos de los fármacos , Neuroglía/metabolismo , Traumatismos de la Médula Espinal/patología , Relación Estructura-Actividad , Pez Cebra/fisiología
19.
Cell Tissue Res ; 364(1): 199-207, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26846113

RESUMEN

Numerous investigations have focused on the detection of antisperm antibodies, which have a naturally occurring impact on male and female fertility. In this study, spermatogenic glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) was considered to be a candidate biomarker of immune infertility. The concentrations of anti-GAPDHS antibodies in the sera of sterile individuals and fertile couples were measured by enzyme-linked immunosorbent assay. Sera were collected from immune infertile (n = 175) and fertile (n = 237) individuals and were screened by tray agglutination tests (TAT). Infertile sera were further divided into two groups according to the serum titers obtained by TAT (titers ≤ 1:8, n = 58; titers > 1:8, n = 117). The concentrations of anti-GAPDHS antibodies were significantly higher in the immune infertile group than in the fertile group and were much higher with regard to the increased degrees of sperm agglutination (titers > 1:8). Surprisingly, we found statistically significantly higher concentrations of antibodies in the sera of infertile men than in those of infertile women, and a similar statistical result was obtained in the sera when primary infertility was compared with secondary infertility. Thus, anti-GAPDHS antibodies seem to be a sensitive parameter in immune infertile detection and might be one of the main factors causing immune infertility. This factor might be valuable as an indicator in the clinical diagnosis and monitoring treatment of infertility.


Asunto(s)
Autoanticuerpos , Gliceraldehído-3-Fosfato Deshidrogenasas/inmunología , Infertilidad Femenina , Infertilidad Masculina , Adulto , Autoanticuerpos/sangre , Autoanticuerpos/inmunología , Biomarcadores/sangre , Femenino , Humanos , Infertilidad Femenina/sangre , Infertilidad Femenina/inmunología , Infertilidad Masculina/sangre , Infertilidad Masculina/inmunología , Masculino , Persona de Mediana Edad
20.
Mol Reprod Dev ; 83(5): 442-54, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-27031046

RESUMEN

Trim69 contains the hallmark domains of a tripartite motif (TRIM) protein, including a Ring-finger domain, B-box domain, and coiled-coil domain. Trim69 is structurally and evolutionarily conserved in zebrafish, mouse, rat, human, and chimpanzee. The role of this protein is unclear, however, so we investigated its function in zebrafish development. Trim69 is extensively expressed in zebrafish adults and developing embryos-particularly in the testis, brain, ovary, and heart-and its expression decreases in a time- and stage-dependent manner. Loss of trim69 in zebrafish induces apoptosis and activates apoptosis-related processes; indeed, the tp53 pathway was up-regulated in response to the knockdown. Expression of human trim69 rescued the apoptotic phenotype, while overexpression of trim69 does not increase cellular apoptosis. Taken together, our results suggest that trim69 participates in tp53-mediated apoptosis during zebrafish development. Mol. Reprod. Dev. 83: 442-454, 2016. © 2016 Wiley Periodicals, Inc.


Asunto(s)
Apoptosis/fisiología , Embrión no Mamífero/embriología , Desarrollo Embrionario/fisiología , Proteínas de Motivos Tripartitos/metabolismo , Proteína p53 Supresora de Tumor/metabolismo , Proteínas de Pez Cebra/metabolismo , Pez Cebra/embriología , Animales , Animales Modificados Genéticamente/embriología , Animales Modificados Genéticamente/genética , Proteínas de Motivos Tripartitos/genética , Proteína p53 Supresora de Tumor/genética , Ubiquitina-Proteína Ligasas/genética , Ubiquitina-Proteína Ligasas/metabolismo , Pez Cebra/genética , Proteínas de Pez Cebra/genética
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