Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 61
Filtrar
Más filtros

Bases de datos
País/Región como asunto
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Entropy (Basel) ; 26(10)2024 Sep 25.
Artículo en Inglés | MEDLINE | ID: mdl-39451894

RESUMEN

Sleep position recognition plays a crucial role in enhancing individual sleep quality and addressing sleep-related disorders. However, the conventional non-invasive technology for recognizing sleep positions tends to be limited in its widespread application due to high production and computing costs. To address this issue, an enhanced stacking model is proposed based on a specific air bag mattress. Firstly, the hyperparameters of the candidate base model are optimized using the Bayesian optimization algorithm. Subsequently, the entropy weight method is employed to select extreme gradient boosting (XGBoost), support vector machine (SVM), and deep neural decision tree (DNDT) as the first layer of the enhanced stacking model, with logistic regression serving as the meta-learner in the second layer. Comparative analysis with existing machine learning techniques demonstrates that the proposed enhanced stacking model achieves higher classification accuracy and applicability.

2.
Funct Integr Genomics ; 23(3): 275, 2023 Aug 18.
Artículo en Inglés | MEDLINE | ID: mdl-37596430

RESUMEN

Liver metastasis of colorectal cancer (CRC) is a major cause of cancer morbidity and mortality. Circular RNAs (circRNAs) have been widely reported to be implicated in cancer metastasis. This study aims to investigate the effect of circSP5 (has_circ_0057010) on liver metastasis of CRC. Quantitative real-time PCR (RT-qPCR) analysis was performed to detect gene expression. The level of proteins was measured by western blot. The migration and invasion of CRC cells were assessed by wound healing assay and transwell assay. In vivo assays were performed after the construction of the CRC xenograft model and CRC model with liver metastasis. Mechanism analyses were performed via RNA-binding protein immunoprecipitation (RIP), RNA pulldown, luciferase reporter, chromatin immunoprecipitation (ChIP), and DNA pulldown assays. We found that circSP5 is significantly overexpressed in CRC with liver metastasis and its depletion suppresses the progression of CRC with liver metastasis in vitro and in vivo. Moreover, circSP5 enhances the expression of Sp5 transcription factor (SP5) via competitively sponging microRNA (miR)-1249-3p and could regulate BMP and activin membrane-bound inhibitor (BAMBI) via transcriptional activation. CircSP5 promotes the migration, invasion, and epithelial-mesenchymal transition (EMT) of CRC cells via BAMBI. In sum, circSP5 promotes liver metastasis of CRC by up-regulating SP5-mediated BAMBI transcription.


Asunto(s)
Neoplasias Colorrectales , Neoplasias Hepáticas , Animales , Humanos , Neoplasias Colorrectales/genética , Modelos Animales de Enfermedad , Neoplasias Hepáticas/genética , Proteínas de la Membrana , ARN , ARN Circular/genética
3.
Surg Endosc ; 37(11): 8498-8510, 2023 11.
Artículo en Inglés | MEDLINE | ID: mdl-37770606

RESUMEN

BACKGROUND: Extragastrointestinal stromal tumors (EGISTs) are rare mesenchymal neoplasms that originate outside the gastrointestinal tract. However, the population-level survival analysis of EGIST remains poorly grasped. Therefore, we aimed to analyze the survival of EGIST patients using the Surveillance, Epidemiology, and End Results (SEER) database. METHODS: All patients diagnosed with GIST and EGIST between 2000 and 2019 were identified through utilization of the SEER database. Missing data were handled using multiple imputation methodology. Kaplan-Meier analyses and Cox proportional hazard models were employed to assess the influence of demographic and clinical characteristics on both overall survival (OS) and cancer-specific survival (CSS). RESULTS: A total of 13,330 patients were enrolled in the study, comprising 12,627 diagnosed with GIST and 703 with EGIST. EGIST patients demonstrated significantly poorer OS [hazard ratio (HR) 1.732, 95% confidence interval (CI) 1.522-1.970, P < 0.001] and CSS (HR 2.167, 95% CI 1.821-2.577, P < 0.001) compared to GIST patients. The mean 1-year, 3-year, 5-year, and 10-year OS rates for EGIST patients were 78.3%, 61.9%, 50.5%, and 32.5%, respectively, with corresponding mean CSS rates of 84.3%, 70.8%, 61.3%, and 46.5%. Multivariate Cox regression analysis identified age, race, sex, grade, size, and surgical type as independent risk factors for OS in EGIST patients, while age, sex, year of diagnosis, grade, surgical type, and radiation therapy were identified as independent risk factors for CSS. Patients with EGIST who underwent surgical treatment exhibited significantly higher 5-year OS rates (49.0% vs. 39.9%, P = 0.035) and CSS rates (63.9% vs. 53.0%, P = 0.028) compared to those who did not undergo surgery. CONCLUSIONS: EGIST patients have a poorer prognosis compared to GIST patients; however, surgical treatment has been shown to improve the prognosis.


Asunto(s)
Tumores del Estroma Gastrointestinal , Humanos , Tumores del Estroma Gastrointestinal/patología , Análisis de Supervivencia , Pronóstico , Modelos de Riesgos Proporcionales , Estimación de Kaplan-Meier , Programa de VERF
4.
Rapid Commun Mass Spectrom ; 34(7): e8658, 2020 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-31733129

RESUMEN

RATIONALE: Molybdenum (Mo) is predominantly expelled from the human body in urine. Consequently, urinary variability in the concentration and isotopic composition of Mo may encode valuable clinical information. To access this information, however, it is first necessary to develop and demonstrate a rapid, accurate and precise methodology capable of concentrating Mo from urine for isotope analysis. METHODS: The utility of N-benzoyl-N-phenylhydroxylamine (BPHA) to effectively separate and purify Mo from urine samples without the need for acid digestion was tested. Following this approach, applying a double-spike mass bias correction, we determined the Mo isotopic compositions of a set of urine samples by multiple collector inductively coupled plasma mass spectrometry (MC-ICP-MS). RESULTS: Based on replicate analyses of an in-house urine standard, this approach demonstrates an external precision on δ98/95 Mo values of better than 0.08‰ (2SD, n = 15). Application to a sample set collected from healthy individuals in Guangzhou, China, provides the first suite of δ98/95 Mo measurements from urine samples. Samples from the female participants show δ98/95 Mo (‰) values (1.31 ± 0.19‰, Ave ± 2SD, n = 14) that are consistently lower than those from the male participants (1.55 ± 0.16‰, Ave ± 2SD, n = 17). CONCLUSIONS: The employed methodology is suitable for rapid, low-blank and high-throughput Mo isotope analysis of urine samples. Although resolvable δ98/95 Mo variability is seen in this preliminary dataset, the mechanism driving this variability is unknown. High-precision Mo isotopic analysis might be added to the urinalysis tool-kit, with the potential to provide valuable clinical information in the future.


Asunto(s)
Molibdeno/orina , Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Hidroxilaminas/química , Límite de Detección , Masculino , Espectrometría de Masas/métodos , Persona de Mediana Edad , Molibdeno/aislamiento & purificación , Urinálisis/métodos , Adulto Joven
5.
Pharmazie ; 74(9): 547-552, 2019 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-31484595

RESUMEN

Clopidogrel-induced gastric injury is an important clinical problem. However, the exact mechanism was still unclarified. This study aimed to investigate the role of high mobility group box protein 1 (HMGB1) and the toll-like receptor 4 (TLR4) pathway in normal human gastric epithelial (GES-1) cells under clopidogrel exposure. Morphological alterations of the gastric epithelial cells were observed under a microscope. A laser scanning confocal microscope was used to determine the distribution of HMGB1 and TLR4 in clopidogrel-induced injury. MTT was used to compare the viability of GES-1 cell among the pretreated Cli-095 (TLR4 inhibitor) group, pretreated ethyl pyruvate (HMGB1 inhibitor) group, clopidogrel group, and control group. Moreover, expression of the HMGB1, TLR4, tight junction (TJ) proteins occludin and the phosphorylation of the mitogen-activated protein kinases (MAPK) p38 were examined by western blot. We found the expression of HMGB1 and TLR4 in the cytoplasm increased after clopidogrel administration. Besides, inhibited TLR4, which is a receptor of HMGB1, prevented the injury and occludin reducing caused by clopidogrel challenge. Furthermore, blocking HMGB1 or TLR4 activity by ethyl pyruvate (HMGB1 inhibitor) or cli-095(TLR4 inhibitor) can partially diminish the activation of p38MAPK. Gastric mucosal damages observed by clopidogrel challenge are associated with HMGB1, TLR4, through p38MAPK signal pathway.


Asunto(s)
Clopidogrel/toxicidad , Células Epiteliales/efectos de los fármacos , Mucosa Gástrica/efectos de los fármacos , Inhibidores de Agregación Plaquetaria/toxicidad , Línea Celular , Supervivencia Celular/efectos de los fármacos , Citoplasma/efectos de los fármacos , Citoplasma/metabolismo , Células Epiteliales/patología , Mucosa Gástrica/patología , Proteína HMGB1/metabolismo , Humanos , Transducción de Señal/efectos de los fármacos , Receptor Toll-Like 4/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo
6.
Genet Sel Evol ; 50(1): 68, 2018 Dec 20.
Artículo en Inglés | MEDLINE | ID: mdl-30572816

RESUMEN

BACKGROUND: Highly diversified in morphology and structure, feathers have evolved into various forms. Frizzle feathers, which result from a developmental defect of the feather, are observed in several domestic chicken breeds. The frizzle phenotype is consistent with incomplete dominance of a major gene, but the molecular mechanisms that underlie this phenotype remain obscure. Kirin, a Chinese indigenous chicken breed that originated in the Guangdong province, is famous for its frizzle feathers. The KRT75 gene is considered as the dominant gene responsible for the frizzle trait in several chicken breeds, but this is not the case in the Kirin breed. Thus, the objective of our study was to investigate the genomic region and mutation responsible for this phenotype in this particular breed. RESULTS: A resource population was produced by crossing Kirin and Huaixiang chickens to produce F1 and F2 generations. DNA samples from 75 frizzle feather and normal feather individuals were sequenced with double-digest genotyping by sequencing (dd-GBS). After the detection of 525,561 high-quality variants, a genome-wide association analysis was carried out and the gene responsible for the frizzle phenotype was localized within the type II α-keratin cluster on chromosome 33. Sanger sequencing was used to screen for mutations in the exons of five genes of this type II α-keratin cluster. A 15-bp deletion in exon 3 of KRT75L4 that showed complete segregation with the frizzle phenotype was detected within the F2 population. Transcriptome sequencing demonstrated that KRT75L4 was expressed but that the transcript was shorter in Kirin than in Huaixiang chickens. In addition, by using Sanger sequencing, we were able to confirm that the deletion was in complete linkage with frizzle feathers. CONCLUSIONS: A deletion in the KRT75L4 gene is responsible for the frizzle feather phenotype in the Kirin chicken. The identification of this mutation, which causes a developmental defect of avian integument appendages, will improve our understanding of the mechanisms that are involved in feather formation.


Asunto(s)
Pollos/genética , Queratina-6/genética , Queratinas/genética , Animales , Secuencia de Bases , China , Exones , Plumas/patología , Genoma/genética , Estudio de Asociación del Genoma Completo/métodos , Genómica/métodos , Genotipo , Fenotipo , Eliminación de Secuencia
7.
Future Oncol ; 14(17): 1731-1739, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29956566

RESUMEN

AIM: To determine whether genetic variations in DNMT1 and DNMT3A could be associated with gastric cancer risk. MATERIALS & METHODS: A total of 466 patients and 452 healthy controls were enrolled in this study. Genotypes were examined by using the Sequenom MassARRAY platform. RESULTS: No significant differences in the distribution frequencies were observed between the groups. However, subgroup analysis revealed that, in the group aged ≤60 years, DNMT3A rs13420827 was associated with reduced gastric cancer risk, and that carrier of GC/GG genotype was associated with reduced risk of gastric cancer with low differentiation or at the T3-T4 stage. CONCLUSION: This study suggests that five genetic variations of interest in DNMT1 and DNMT3A are not associated with the presence of gastric cancer, but that rs13420827 may contribute to the gastric cancer risk for those younger individuals, the risk of which may be influenced by the characteristics of tumor.


Asunto(s)
Pueblo Asiatico/genética , Biomarcadores de Tumor/genética , ADN (Citosina-5-)-Metiltransferasa 1/genética , ADN (Citosina-5-)-Metiltransferasas/genética , Polimorfismo de Nucleótido Simple , Neoplasias Gástricas/genética , Estudios de Casos y Controles , China/epidemiología , ADN Metiltransferasa 3A , Femenino , Estudios de Seguimiento , Predisposición Genética a la Enfermedad , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Pronóstico , Neoplasias Gástricas/epidemiología , Tasa de Supervivencia
8.
Anal Bioanal Chem ; 408(12): 3359-67, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-26894762

RESUMEN

Dispersive liquid-liquid microextraction combined with collection of the extraction phase with the hollow fiber was applied to the extraction of estrogens from environmental water samples. 1-Undecanol with relatively lower toxicity was used as the extraction solvent. The hollow fiber was used to collect the extraction phase containing the analytes from the aqueous phase. Hollow fibers collecting the extraction phase were eluted with acetonitrile and the resulting eluate was analyzed by high performance liquid chromatography. Several parameters, including pH of sample, the type and volume of the extraction and dispersive solvent, salt concentration, extraction time, and collection time were optimized. Under the optimal experimental conditions, the limits of detection for estriol, 17α-estradiol, and ethynylestradiol were 4.58, 1.41, and 1.41 µg L(-1), respectively. When the present method was applied to the analysis of real water samples, the recoveries of estrogens at two spiked levels were in the range of 55.8-107.4%. In this method, the separation of the extraction phase and aqueous phase becomes easy with no need for centrifugation, refrigeration-thaw, or any special device. The hollow fiber was commercially available and the collection procedure was easy to perform, which make the present method have potential for automation and wide promotion. Small sizes of pores on the walls of the hollow fibers can block large molecules, which makes the present method have the potential for the treatment of complex matrices.

9.
Zhonghua Wai Ke Za Zhi ; 54(4): 286-91, 2016 Apr 01.
Artículo en Zh | MEDLINE | ID: mdl-27029204

RESUMEN

OBJECTIVE: To investigate a method that constructing a tissue-engineered tendon with a continuous and heterogeneous transition region. METHODS: Fibroblasts derived from rabbit epithelial tissue were cultured in vitro and collagen gel was prepared. The experimental groups were scaffold only group, fibroblasts+ chondrocytes group (Fb+ CC group), fibroblasts+ osteoblasts group (Fb+ OB group), fibroblasts+ chondrocytes+ osteoblasts group (Fb+ CC+ OB group). Heterogeneous cell populations(fibroblasts, chondrocytes and osteoblasts) with collagen gel were seeded within three predesigned specific regions (fibrogenesis, chondrogenesis, and osteogenesis) of decellularized rabbit achilles tendons to fabricate a stratified scaffold containing three biofunctional regions supporting fibrogenesis, chondrogenesis, and osteogenesis. The tests of morphology, architecture and cytocompatibility of the scaffolds were performed. Gradient tissue-specific matrix formation was analysed within the predesignated regions via histological staining and immunofluorescence assays. RESULTS: The HE staining and scanning electron microscopy analysis demonstrated that no major cell fragments or nuclear material was evident, and increased intra-fascicular and inter-fascicular spaces were found, the cytocompatibility of the scaffolds showed that the numbers of viable cells on the scaffold surfaces increase steadily, no significant differences were found between the scaffold only containing ordinary culture medium and scaffold containing gel groups. Histological staining and immunofluorescence assays demonstrated that the cartilage-related markers (GAG, COL2A1) were found only in the chondrogenesis region, but bone-related proteins only in the osteogenesis region of bone tunnel, and fibrosis was remarkable for the fibrogenesis region in the joint cavity. The transitional architecture with ligament-fibrocartilage-bone was constructed in the ligament-bone tunnel interface. CONCLUSIONS: A transitional interface (fiber-fiberocartilage-bone) could be replicated in a decellularized tendon through stratified tissue integration in vitro. The cell-tendon complex offers the advantages of a multi-tissue transition involving controlled cellular interactions and matrix heterogeneity.


Asunto(s)
Tendones , Ingeniería de Tejidos/métodos , Animales , Huesos , Células Cultivadas , Condrocitos/citología , Colágeno , Fibroblastos/citología , Ligamentos , Osteoblastos/citología , Conejos
10.
J Sep Sci ; 38(23): 4127-35, 2015 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-26450655

RESUMEN

Matrix solid-phase dispersion coupled with homogeneous ionic liquid microextraction was developed and applied to the extraction of some sulfonamides, including sulfamerazine, sulfamethazine, sulfathiazole, sulfachloropyridazine, sulfadoxine, sulfisoxazole, and sulfaphenazole, in animal tissues. High-performance liquid chromatography was applied to the separation and determination of the target analytes. The solid sample was directly treated by matrix solid-phase dispersion and the eluate obtained was treated by homogeneous ionic liquid microextraction. The ionic liquid was used as the extraction solvent in this method, which may result in the improvement of the recoveries of the target analytes. To avoid using organic solvent and reduce environmental pollution, water was used as the elution solvent of matrix solid-phase dispersion. The effects of the experimental parameters on recoveries, including the type and volume of ionic liquid, type of dispersant, ratio of sample to dispersant, pH value of elution solvent, volume of elution solvent, amount of salt in eluate, amount of ion-pairing agent (NH4 PF6 ), and centrifuging time, were evaluated. When the present method was applied to the analysis of animal tissues, the recoveries of the analytes ranged from 85.4 to 118.0%, and the relative standard deviations were lower than 9.30%. The detection limits for the analytes were 4.3-13.4 µg/kg.


Asunto(s)
Técnicas de Química Analítica , Cromatografía Líquida de Alta Presión , Líquidos Iónicos/química , Microextracción en Fase Líquida , Sulfonamidas/análisis , Animales , Riñón/química , Límite de Detección , Hígado/química , Estructura Molecular , Músculos/química
11.
Front Med (Lausanne) ; 11: 1361777, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38725470

RESUMEN

Background: High altitudes are characterized by low-pressure oxygen deprivation. This is further exacerbated with increasing altitude. High altitudes can be associated with reduced oxygenation, which in turn, can affect labor, as well as maternal and fetal outcomes. Epidural anesthesia can significantly relieve labor pain. This study aimed to assess the effects of elevation gradient changes at high altitude on the analgesic effect of epidural anesthesia, labor duration, and neonatal outcomes. Methods: We divided 211 women who received epidural anesthesia into groups according to varying elevation of their residence (76 in Xining City, mean altitude 2,200 m; 63 in Haibei Prefecture, mean altitude 3,655 m; and 72 in Yushu Prefecture, mean altitude 4,493 m). The analgesic effect was assessed using a visual analog scale (VAS). Labor duration was objectively recorded. The neonatal outcome was assessed using Apgar scores and fetal umbilical artery blood pH. Results: VAS scores among the three groups did not differ significantly (p > 0.05). The neonatal Apgar scores in descending order were: Xining group > Haibei group > Yushu group (p < 0.05). The stage of labor was similar among the three groups (p > 0.05). Fetal umbilical artery blood pH in descending order were: Xining group > Haibei group > Yushu group (p < 0.05). Conclusion: Elevation gradient changes in highland areas did not affect the efficacy of epidural anesthesia or labor duration. However, neonatal outcomes were affected.

12.
Food Chem ; 460(Pt 1): 140525, 2024 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-39047472

RESUMEN

The determination of vitamin B6 (VB6) in food is of great significance due to its vital role in maintaining health and its necessity for ingestion through dietary sources. Therefore, based on ionic liquid-based yellow-emitting carbon dots (Y-CDs), a novel fluorescence-smartphone dual-mode method was first developed. The present method was applied to the detection of VB6 in milk. In the fluorescence method, the formation of complexes between VB6 and Y-CDs results in a significant decrease of the fluorescence intensity of Y-CDs. VB6 in milk samples was successfully determined according to this method, which exhibited a low detection limit (5 × 10-5 mg/mL) and excellent recoveries (98.80%-103.80%), demonstrating its feasibility in real sample analysis. In addition, the smartphone-based analysis method was established by researching the correlation between different VB6 concentrations and the (R + B) values of Y-CDs. When this method was applied, the detection process of VB6 was simplified. By combining the two methods, the possibility of incorrect analysis results can be effectively reduced, and the reliability of detection results can be improved through cross-validation of the two methods. Compared with traditional chromatography and electrochemical methods, the dual-mode method was more rapid, convenient, accurate, and suitable for the detection of VB6.


Asunto(s)
Carbono , Líquidos Iónicos , Leche , Puntos Cuánticos , Teléfono Inteligente , Vitamina B 6 , Leche/química , Animales , Carbono/química , Puntos Cuánticos/química , Líquidos Iónicos/química , Vitamina B 6/análisis , Fluorescencia , Límite de Detección , Espectrometría de Fluorescencia/métodos , Contaminación de Alimentos/análisis , Bovinos
13.
J Chromatogr A ; 1717: 464692, 2024 Feb 22.
Artículo en Inglés | MEDLINE | ID: mdl-38320432

RESUMEN

A simple, fast, and efficient ultrasonic-assisted supramolecular solvent microextraction combined with high performance liquid chromatography method was developed for the determination of coumarins in Cortex fraxini, including esculin, esculetin and fraxetin. In this study, a novel supramolecular solvent was prepared with 1-octanol, tetrahydrofuran and water for the first time, and its composition, viscosity, density, structure, and micromorphology were characterized. The prepared supramolecular solvent exhibited vesicular structures and had the characteristics of low viscosity. Through single-factor experiments, response surface methodology and artificial neural network-genetic algorithm, the optimal extraction conditions were obtained as follows: NaCl concentration of 1 mol mL-1, pH value of 10, solid-liquid ratio of 10:1, vortex time of 30 s, ultrasonic power of 100 W, ultrasonic temperature of 60 °C, ultrasonic time of 15 min, centrifugation speed of 5000 rpm, and centrifugation time of 1 min. The results demonstrated that the artificial neural network model exhibited maximum R-values of 0.98703, 0.97440, 0.99836, and 0.95447 for training, testing, validation, and all dataset, respectively. The minimum mean square errors were 0.75, 10.15, 1.99, and 2.63, respectively. This indicated that the predicted values were almost consistent with the actual values. Under the optimal conditions, the total extraction yields of target analytes reached 2.80 %. The calibration curves for each analyte exhibited excellent linearity within the linear range (r > 0.9993). The limits of detection and quantification ranged from 4.87 to 6.55 ng mL-1 and 16.24 to 21.84 ng mL-1, respectively. The recoveries ranged from 98.71 % to 111.01 % with relative standard deviations of less than 3.6 %. The present method had the advantages of short extraction time (15 min) and less solvent consumption (0.5 mL). The prepared supramolecular solvent was proved to have great potential in extracting coumarins from medicinal plants.


Asunto(s)
Medicamentos Herbarios Chinos , Microextracción en Fase Líquida , Solventes/química , Ultrasonido , Microextracción en Fase Líquida/métodos , Cumarinas , Medicamentos Herbarios Chinos/química , Cromatografía Líquida de Alta Presión/métodos , Algoritmos , Límite de Detección
14.
Food Chem ; 450: 139298, 2024 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-38615532

RESUMEN

A convenient, efficient, and green dispersive liquid-liquid microextraction based on the in situ formation of solidified supramolecular solvents combined with high performance liquid chromatography was developed for the determination of four phenylurea herbicides in liquid samples, including monuron, monolinuron, isoproturon, and chlortoluron. Herein, a novel supramolecular solvent was prepared by the in situ reaction of [P4448]Br and NH4PF6, which had the advantages of low melting point, high density, and good dispersibility. In addition, the microscopic morphology and physical properties of supramolecular solvent were characterized, and the extraction conditions were optimized. The results showed that the analytes had good linearity (R2 > 0.9998) within the linear range. The limits of detection and quantification for the four phenylurea herbicides were in the range of 0.13-0.19 µg L-1 and 0.45-0.65 µg L-1, respectively. The prepared supramolecular solvent is suitable for the efficient extraction of phenylurea herbicides in water, fruit juice, and milk.


Asunto(s)
Jugos de Frutas y Vegetales , Herbicidas , Microextracción en Fase Líquida , Leche , Compuestos de Fenilurea , Solventes , Microextracción en Fase Líquida/métodos , Herbicidas/química , Herbicidas/aislamiento & purificación , Herbicidas/análisis , Leche/química , Compuestos de Fenilurea/aislamiento & purificación , Compuestos de Fenilurea/química , Compuestos de Fenilurea/análisis , Jugos de Frutas y Vegetales/análisis , Solventes/química , Animales , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Contaminación de Alimentos/análisis
15.
Int J Biol Macromol ; 281(Pt 4): 136665, 2024 Oct 16.
Artículo en Inglés | MEDLINE | ID: mdl-39423976

RESUMEN

Salivary pepsin has emerged as a promising biomarker for rapid screening and diagnosis of gastroesophageal reflux disease (GERD). Pepsin mainly exists in strongly acidic environments and exhibits its highest activity. However, the poor stability of most fluorescent sensors in strong acidic environments brings a significant challenge for pepsin detection. Herein, an innovative biosensor was developed for the highly specific and sensitive detection of pepsin on the basis of green-emitting ionic liquid-based carbon dots (G-IL-CDs) conjugated with whey proteins (WPs). The G-IL-CDs exhibited aggregation-induced fluorescence enhancement when interacting with WP, and the fluorescence intensity decreased after incubation with pepsin due to the disruption of the aggregation structure. This strategy is highly selective for pepsin due to the strongly acidic environment in which other proteases are inactivated. Under optimal experimental conditions, this biosensor successfully detected pepsin in real human saliva with a satisfying recovery. Furthermore, this study not only developed a CDs-based sensor for detecting pepsin but also laid a solid theoretical foundation for the future development of novel biosensors combining CDs and proteins.

16.
In Vivo ; 38(1): 213-225, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38148070

RESUMEN

BACKGROUND/AIM: Cisplatin resistance often leads to treatment futility and elevated mortality rates in patients with lung cancer. One promising strategy to address this challenge involves the integration of traditional Chinese medicine (TCM) with chemotherapeutic drugs. Currently, the potential synergistic effect and underlying mechanism of polyphyllin II (PPII) and cisplatin combination in combating cisplatin (DDP) resistance in lung cancer remain unexplored. MATERIALS AND METHODS: In this study, we established a cisplatin resistance model using A549 cells and explored the underlying mechanisms of PPII in combination with cisplatin in A549/DDP resistant cells. Specifically, we assessed the impact of PPII combined with cisplatin on A549/DDP cell proliferation, viability, and the expression of apoptosis-related proteins. To gain deeper insights into the underlying mechanism, we examined the effects of PPII and cisplatin on mitochondrial function in A549/DDP cells. RESULTS: This combination induced cell cycle arrest at both the S phase and G2/M phase in A549/DDP cells, thereby promoting apoptosis. Western blotting confirmed that DDP acted synergistically with PPII to enhance the expression of apoptotic proteins, diminish the expression of anti-apoptotic proteins, and promote the expression of anti-proliferation proteins in the mitochondrial pathway of A549/DDP cells. CONCLUSION: The combination of PPII and cisplatin effectively modulated the mitochondrial function, thereby reversing drug resistance in A549/DDP cells. This innovative combination therapy shows significant promise as a novel strategy for overcoming cisplatin resistance in lung cancer.


Asunto(s)
Antineoplásicos , Neoplasias Pulmonares , Humanos , Cisplatino , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Células A549 , Resistencia a Antineoplásicos/genética , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/metabolismo , Apoptosis , Proteínas Reguladoras de la Apoptosis/metabolismo , Mitocondrias/metabolismo , Metabolismo Energético , Proliferación Celular , Línea Celular Tumoral
17.
J Sep Sci ; 36(21-22): 3527-33, 2013 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24106035

RESUMEN

In this paper, a magnetic bar microextraction was developed to extract schisandrin A, schisantherin A, and deoxyschizandrin from Wuweizi. The analytes were determined by HPLC. A stainless-steel wire was inserted into the hollow of the hollow fiber to make the magnetic bar. The bar can be used to stir the extraction system and extract the analytes, and was isolated from the extract system by magnetic force. Several experimental parameters, including type and volume of extraction solvent, the number of magnetic bars, extraction temperature and time, stirring speed and NaCl concentration were investigated and optimized. The LODs for schisandrin A, schisantherin A, and deoxyschizandrin were 0.14, 0.06, and 0.10 g/mL, respectively. The recoveries were in the range of 70.90-106.67% and the RSDs were < 8.84%. Compared with ultrasound-assisted and Soxhlet extraction, when the present method was applied, the extraction time was shorter, the sample amount was smaller, and the consumption of organic solvent was lower.


Asunto(s)
Lignanos/análisis , Microextracción en Fase Líquida/métodos , Schisandra/química , Cromatografía Líquida de Alta Presión , Magnetismo
18.
J Sep Sci ; 36(14): 2348-57, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23677705

RESUMEN

Nine nucleosides and nucleobases, including uracil, adenine, thymine, uridine, adenosine, thymidine, cytidine, guanosine, and cordycepin in natural Cordyceps sinensis, cultured Cordyceps mycelia, and Cordyceps fruiting bodies were extracted by matrix solid-phase dispersion (MSPD) and determined by HPLC. The experimental conditions for the MSPD extraction were optimized. Florisil was used as dispersant, petroleum ether as washing solvent, and methanol as elution solvent. The Florisil-to-sample ratio was selected to be 4:1 and no additional clean-up sorbent was needed. The calibration curves had good linear relationships (r > 0.9997). The LOD and LOQ were in the range of 12~79 and 41~265 ng/mL, respectively. The intra- and interday precision were lower than 8.3%. The recoveries were between 61.5 and 93.2%. The present method consumed less sample compared with ultrasonic extraction and heating reflux extraction (HRE). The extraction yields obtained by using the present method are much higher than those obtained by UE and comparable to those obtained by HRE.


Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Cordyceps/química , Nucleósidos/análisis , Nucleótidos/análisis , Extractos Vegetales/análisis , Extracción en Fase Sólida/métodos , Nucleósidos/aislamiento & purificación , Nucleótidos/aislamiento & purificación , Extractos Vegetales/aislamiento & purificación
19.
Ann Med ; 55(2): 2261461, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37751485

RESUMEN

BACKGROUND: Previous reports have not reached consistent results regarding the prognostic significance of the controlling nutritional status (CONUT) score in biliary tract cancer (BTC). Therefore, the present meta-analysis was conducted to investigate the precise role of the CONUT score in predicting the prognosis of BTC. METHODS: Electronic platforms including Web of Science, PubMed, Cochrane Library, and Embase were comprehensively searched up to May 2, 2023. We also determined combined hazard ratios (HRs) and 95% confidence intervals (CIs) to estimate the role of the CONUT score in predicting the prognosis of patients with BTC. RESULTS: Ten studies involving 1,441 patients were included in the present study. Nine studies treated patients with surgical resection, and one study used percutaneous transhepatic biliary stenting (PTBS) plus 125I seed intracavitary irradiation. Based on the combined data, a higher CONUT score significantly predicted dismal overall survival (OS) (HR = 1.94, 95%CI = 1.41-2.66, p < 0.001), inferior recurrence-free survival (RFS) (HR = 1.79, 95%CI = 1.48-2.17, p < 0.001) in BTC, and low differentiation (OR = 1.57, 95%CI = 1.15-2.14, p = 0.004). Nonetheless, the CONUT score was not related to sex, lymph node metastasis, microvascular invasion, perineural invasion, distant metastasis, TNM stage, or tumor number in patients with BTC. CONCLUSION: Higher CONUT scores significantly predicted worse OS and RFS in patients with BTC. Moreover, BTC patients with high CONUT scores tended to have poor tumor differentiation. The CONUT score could help clinicians stratify high-risk patients with BTC and devise individualized treatment plans.


As far as we know, this study is the first to analyze whether pretreatment CONUT is significant for predicting the prognosis of BTC.A high CONUT significantly predicted worse OS and RFS in BTC patients.CONUT could help clinicians stratify high-risk BTC patients and devise individualized treatment plans.


Asunto(s)
Neoplasias del Sistema Biliar , Radioisótopos de Yodo , Humanos , Estado Nutricional , Pronóstico , Neoplasias del Sistema Biliar/terapia
20.
Front Cell Infect Microbiol ; 13: 1294379, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-38089809

RESUMEN

Background: The increasing prevalence of antibiotic resistance in cases of Helicobacter pylori (H. pylori) infection has emerged as a significant global issue. This study offers a comprehensive examination of the alterations in drug resistance exhibited by H. pylori in the Nanjing region of China during the preceding five years. Another important objective is to investigate the influence of levofloxacin medication history on genotypic and phenotypic resistance. Methods: This research screened 4277 individuals diagnosed with H. pylori infection between April 2018 and May 2023. The phenotype and genotypic resistance were evaluated using the Kirby-Bauer disk diffusion and PCR method. Results: The most recent primary resistance rates for metronidazole, clarithromycin, levofloxacin, amoxicillin, furazolidone, and tetracycline were recorded at 77.23% (2385/3088), 37.24% (1150/3088), 27.72% (856/3088), 0.52% (16/3088), 0.19% (6/3088), and 0.06% (2/3088), respectively. For the recent five years, we observed a notable upsurge in the rate of metronidazole resistance and a slight elevation of clarithromycin and levofloxacin resistance. The documented resistance rates to single-drug, dual-drug, triple-drug, and quadruple-drug regimens were 35.39%, 28.32%, 25.72%, and 0.21%, respectively. The prevalence of multidrug-resistant strains escalated, rising from 37.96% in 2018 to 66.22% in 2023. The rate of phenotypic and genotypic resistance rate (57.10% and 65.57%) observed in strains obtained from patients without a levofloxacin treatment history was significantly lower than the rate in strains obtained from those with a history of levofloxacin treatment (88.73% and 94.74%). The prevailing gyrA mutations were primarily N87K (52.35%, 345/659), accompanied by D91N (13.96%, 92/659), and closely followed by D87G (10.77%, 71/659). For gyrA mutations, the 91-amino acid mutants exhibit a higher likelihood of discrepancies between phenotypic and genotypic resistance than the 87-amino acid mutants. Conclusion: The extent of antibiotic resistance within H. pylori remains substantial within the Nanjing region. If levofloxacin proves ineffective in eradicating H. pylori during the initial treatment, its use in subsequent treatments is discouraged. The employment of levofloxacin resistance genotype testing can partially substitute conventional antibiotic sensitivity testing. Notably, predicting phenotypic resistance of levofloxacin through PCR requires more attention to the mutation type of gyrA to improve prediction accuracy.


Asunto(s)
Infecciones por Helicobacter , Helicobacter pylori , Humanos , Claritromicina/farmacología , Claritromicina/uso terapéutico , Metronidazol/farmacología , Metronidazol/uso terapéutico , Helicobacter pylori/genética , Levofloxacino/farmacología , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Infecciones por Helicobacter/tratamiento farmacológico , Infecciones por Helicobacter/epidemiología , Farmacorresistencia Microbiana , China/epidemiología , Aminoácidos/farmacología , Farmacorresistencia Bacteriana/genética , Pruebas de Sensibilidad Microbiana
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA