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1.
Sci Adv ; 10(25): eadn0014, 2024 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-38905346

RESUMEN

The central nervous system coordinates peripheral cellular stress responses, including the unfolded protein response of the mitochondria (UPRMT); however, the contexts for which this regulatory capability evolved are unknown. UPRMT is up-regulated upon pathogenic infection and in metabolic flux, and the olfactory nervous system has been shown to regulate pathogen resistance and peripheral metabolic activity. Therefore, we asked whether the olfactory nervous system in Caenorhabditis elegans controls the UPRMT cell nonautonomously. We found that silencing a single inhibitory olfactory neuron pair, AWC, led to robust induction of UPRMT and reduction of oxidative phosphorylation dependent on serotonin signaling and parkin-mediated mitophagy. Further, AWC ablation confers resistance to the pathogenic bacteria Pseudomonas aeruginosa partially dependent on the UPRMT transcription factor atfs-1 and fully dependent on mitophagy machinery. These data illustrate a role for the olfactory nervous system in regulating whole-organism mitochondrial dynamics, perhaps in preparation for postprandial metabolic stress or pathogenic infection.


Asunto(s)
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans , Mitocondrias , Mitofagia , Olfato , Animales , Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/fisiología , Mitocondrias/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/genética , Olfato/fisiología , Respuesta de Proteína Desplegada , Pseudomonas aeruginosa/fisiología , Ubiquitina-Proteína Ligasas/metabolismo , Ubiquitina-Proteína Ligasas/genética , Fosforilación Oxidativa , Transducción de Señal , Serotonina/metabolismo , Factores de Transcripción
2.
Cell Rep Methods ; 3(4): 100455, 2023 04 24.
Artículo en Inglés | MEDLINE | ID: mdl-37159672

RESUMEN

Brain glucose metabolism is highly heterogeneous among brain regions and continues postmortem. In particular, we demonstrate exhaustion of glycogen and glucose and an increase in lactate production during conventional rapid brain resection and preservation by liquid nitrogen. In contrast, we show that these postmortem changes are not observed with simultaneous animal sacrifice and in situ fixation with focused, high-power microwave. We further employ microwave fixation to define brain glucose metabolism in the mouse model of streptozotocin-induced type 1 diabetes. Using both total pool and isotope tracing analyses, we identified global glucose hypometabolism in multiple brain regions, evidenced by reduced 13C enrichment into glycogen, glycolysis, and the tricarboxylic acid (TCA) cycle. Reduced glucose metabolism correlated with a marked decrease in GLUT2 expression and several metabolic enzymes in unique brain regions. In conclusion, our study supports the incorporation of microwave fixation for more accurate studies of brain metabolism in rodent models.


Asunto(s)
Encéfalo , Microondas , Animales , Ratones , Encéfalo/diagnóstico por imagen , Metaboloma , Glucosa , Glucógeno
3.
EMBO Mol Med ; 14(11): e16029, 2022 11 08.
Artículo en Inglés | MEDLINE | ID: mdl-36059248

RESUMEN

Glycogen dysregulation is a hallmark of aging, and aberrant glycogen drives metabolic reprogramming and pathogenesis in multiple diseases. However, glycogen heterogeneity in healthy and diseased tissues remains largely unknown. Herein, we describe a method to define spatial glycogen architecture in mouse and human tissues using matrix-assisted laser desorption/ionization mass spectrometry imaging. This assay provides robust and sensitive spatial glycogen quantification and architecture characterization in the brain, liver, kidney, testis, lung, bladder, and even the bone. Armed with this tool, we interrogated glycogen spatial distribution and architecture in different types of human cancers. We demonstrate that glycogen stores and architecture are heterogeneous among diseases. Additionally, we observe unique hyperphosphorylated glycogen accumulation in Ewing sarcoma, a pediatric bone cancer. Using preclinical models, we correct glycogen hyperphosphorylation in Ewing sarcoma through genetic and pharmacological interventions that ablate in vivo tumor growth, demonstrating the clinical therapeutic potential of targeting glycogen in Ewing sarcoma.


Asunto(s)
Neoplasias Óseas , Osteosarcoma , Sarcoma de Ewing , Masculino , Humanos , Animales , Ratones , Niño , Sarcoma de Ewing/patología , Glucógeno , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos
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