First Report of Alternaria tenuissimain Causing Leaf Spot of Celery (Apium graveolens) in China.

Celery (Apium graveolens) is one of the most widely grown vegetables in the world. A survey in Anding District of Gansu Province in 2019 showed that the incidence of celery leaf spot was 25%-45%. The disease mainly occurs in late June and July. The leaf spot is conducive to the onset at high temperature and humidity environment. The initial symptoms were many small light brown, irregular-shaped on the leaves. The lesions gradually enlarged in the later stage of the disease, and multiple lesions coalesced to form large irregular brown spots, eventually the whole leaves died. A 3~4mm leaf tissue was cut from the junction of the diseased leaf and the healthy area, the leaf tisse was surface-sterilized in 1.5% NaClO for 1 min and washed with sterile water. Then, it was incubated on potato dextrose agar (PDA) and obtained the pure culture (Q1). After 5 days of cultivation at 25°C, the fungal colonies were olivaceous to dark olive with white margins and abundant aerial mycelia. The conidia were obclavate or ellipsoid, pale brown, with 3~4 longitudinal septa and 2~7 transverse septa, and measured 20.0 to 50.0 × 3.5 to 14.0µm (n=50). Conidiophores were septate, arising singly, and measured 3.5 to 40.0 × 2.5 to 4.5 µm (n=50). Based on morphological characteristics, the fungus was preliminarily identified as A.tenuissima (Simmons 2007). To further confirm the identification, the internal transcribed spacer region (ITS), translation elongation factor 1-α gene (TEF), RNA polymerase II second largest subunit (RPB2), major allergen Alt a 1 gene (Alt a 1), endopolygalacturonase gene (endoPG), anonymous gene region (OPA10-2) and glyceraldehyde 3-phos-phatedehydrogenase (GAPDH) were amplified and sequenced using primers ITS1/ITS4 (Peever et al. 2004), EF1-728F/EF1-986R (Carbone et al. 1999), RPB2-5F2/RPB2-5R (Sung et al. 2007), Alt-for/Alt-rev (Hong et al. 2005), EPG-specific/EPG-3b (Peever et al. 2004), OPA10-2R/OPA10-2L (Peever et al. 2004) and Gpd1/Gpd2 (Berbee et al. 1999) (GenBank accession no.MN046364, MW016001, MW016002, MW016003, MW016004, MW016005, MW016006). DNA sequences of TEF, RPB2, endoPG, OPA10-2 and GAPDH were 100% identical to those of A. tenuissima (MN256108, MK605866, KP789503, JQ859829 and MK683802), but ITS and Alt a 1 were 100% similarity with A. tenuissima (MN615420, JQ282277) and A. alternate (MT626589, KP123847). The ITS and Alt a 1 sequence did not distinguish A. tenuissima from the A. alternate complex. Maximum likelihood phylogenetic analyses were performed for the combined data set with TEF, RPB2, and endoPG using MEGA6 under the Tamura-Nei model (Kumar et al. 2016). The isolate Q1 clustered with type strain A. tenuissima CBS 918.96. The 20 celery plants of 4-7 leaf age were used test the pathogenicity of Q1, the ten plants were sprayed with 20ml of spore suspension (1×105 spores/ml), the control was sprayed with 20mL sterile water, which were placed in a growth chamber (25℃, a 14h light and 10h dark period, RH > 80%). Eight days after inoculation, 40% of the leaves formed lesions, which were consistent with the field observation，the control group was asymptomatic. The pathogen was reisolated from infected leaves to fulfill Koch's postulates. To our knowledge, this is the first report of A. tenuissima causing leaf spot on celery in China.

Ecophysiological responses of mangrove Kandelia obovata seedlings to bed-cleaning sludge from coastline shrimp ponds.

Cumulative effect of bed-cleaning sludge (BCS) from shrimp ponds on the physiology of Kandelia obovata seedling were investigated. Based on the accumulation rate of BCS discharged from shrimp ponds in mangrove forests, four types of sediment coverage thicknesses (SCT) of 0, 2, 4, and 8 cm were set up. With the increases in SCTs, photosynthetic rate, stomatal conductance, transpiration rates were lowest in SCT8; intercellular CO2 concentrations were lowest in SCT4. Leaf superoxide dismutase and peroxidase activities rose and then fell with the increases in SCTs, and Leaf malonaldehyde contents significantly increased. However, contents of leaf free proline, soluble protein and soluble sugar were lowest for SCT4. Root activity was highest for SCT4. Leaves had high N contents, while roots had high P contents. Overall, as for physiological parameters of K. obovata seedlings, SCTs <4 cm were suitable and the values up to 8 cm formed some stresses.

Biodegradation and Decolorization of Crystal Violet Dye by Cocultivation with Fungi and Bacteria.

Microbial degradation of dyes is vital to understanding the fate of dyes in the environment. In this study, a fungal strain A-3 and a bacterial strain L-6, which were identified as Aspergillus fumigatus and Pseudomonas fluorescens, respectively, had been proven to efficiently degrade crystal violet (CV) dye. The decolorization of CV dye by fungal and bacterial cocultivation was investigated. The results showed that the decolorization rate of cocultures was better than monoculture (P. fluorescens in L-6 (PF), and that of A. fumigatus A-3 (AF)). Furthermore, enzymatic analysis further revealed that Lac, MnP, Lip, and NADH-DCIP reductases were involved in the biodegradation of CV dyes. UV-visible spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS) were used to examine the degradation products. GC-MS analysis showed the presence of 4-(dimethylamino) benzophenone, 3-dimethylaminophenol, benzyl alcohol, and benzaldehyde, indicating that CV was degraded into simpler compounds. The phytotoxicity tests revealed that CV degradation products were less toxic than the parent compounds, indicating that the cocultures detoxified CV dyes. As a result, the cocultures are likely to have a wide range of applications in the bioremediation of CV dyes.

Micro-transplantation in an elderly patient with very high risk MDS:A case report and literature review.

Introduction: The prognosis of patients with myelodysplastic syndromes (MDS) (very high risk) is poor.HLA-mismatched allogeneic T-cell infusion which is called micro-transplantation can not only shorten the time of bone marrow suppression, but also improve the treatment response of patients. Case presentation: A 74-year-old woman presented with fatigue and showed pancytopenia on routine blood count. She was diagnosed with MDS (very high risk) after bone marrow examination,then she received 4 cycles of micro-transplantation. The progression-free survival was 22 months and overall survival was 33 months. Discussion: The patient showed good tolerance to micro-transplantation with manageable toxicities and short myelosuppression time.

Electroacupuncture Before Gastrectomy Accelerates Recovery from Gastrointestinal Dysfunction: A Feasibility Study.

Objective: This study investigated the feasibility and effectiveness of preoperative electroacupuncture (EA), given within 30 minutes before surgery, on postoperative gastrointestinal dysfunction (PGD) in patients undergoing open gastrectomy. Materials and Methods: Patients (N = 60) undergoing open gastrectomy were allocated randomly to a usual care (UC) group (n = 30) or an EA group (n = 30). Patients in the EA group were given bilateral EA on ST-36 (Zusanli), ST-37 (Shangjuxv), and ST-39 (Xiajuxv) within 30 minutes before the surgery. The UC group had no acupuncture treatment. Primary outcomes were feasibility of recruitment, retention, acceptability, and patients' global satisfaction. Secondary outcomes included time to first flatus, defecation, liquid diet, incidence and severity of abdominal distension (AD), and incidence of postoperative nausea (PON) and postoperative vomiting (POV). EA-related adverse events were recorded. Results: Of the 61 recruited patients, 1 declined to participate and 60 were randomized into the 2 study groups. All participants completed the interventions. On the acceptability questionnaire, participants' acceptance of EA was statistically improved after the treatment (P < 0.001). Global satisfaction was higher in the EA group (P < 0.001) at 8 (range: 7-8) versus the UC group at 6 (range: 5-7), and the proportion of patients with at least good satisfaction (numerical scale of more than 7 of 10) reached 80% in the EA group. Compared to the UC group, the EA group had a shorter time to first flatus (EA: 57.67 ± 23.09 hours versus 71.27 ± 17.78 hours; P = 0.013). There were no significant differences in time to first defecation (P = 0.081) and liquid diet (P = 0.068), AD (P = 0.436), PON (P = 0.667), or POV (P = 1.000). EA-related adverse events were similar in the 2 groups (P = 1.000). Conclusions: EA is feasible, acceptable to patients, and associated with higher postoperative satisfaction in patients undergoing open gastrectomy. A large multicentered trial is needed to test the effectiveness of EA on PGD.

Decolourization and biodegradation of methylene blue dye by a ligninolytic enzyme-producing Bacillus thuringiensis: Degradation products and pathway.

In this study, a ligninolytic enzyme-producing strain F5 was isolated and identified as Bacillus thuringiensis, which can efficiently degrade methylene blue (MB) dye. The optimal pH, temperature, rotation speed, NaCl concentration, and inoculum of strain F5 for MB degradation were pH 6.0, 30 °C, 140 rpm, 10 g/L NaCl, 4% inoculum (v/v), and the strain F5 had salt tolerance, the MB decolorization rate reached 95% after 12 h. The degraded products were characterized by UV-vis, FT-IR, and GC-MS. Based on products analysis, four different intermediates were identified, and a new pathway for the degradation of MB was proposed. The degradation of MB by strain F5 was due to the synergistic effects of laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP), and NADH-DCIP reductase; among them, Lac and MnP were the key enzymes. The phytotoxicity results showed that MB degraded metabolites' toxicity was lower than that of the parent compound, indicating that the strain F5 had a detoxification effect on MB dyes.

Non-Ablative Chemotherapy Followed by HLA-Mismatched Allogeneic CD3+ T-Cells Infusion Causes An Augment of T-Cells With Mild CRS: A Multi-Centers Single-Arm Prospective Study on Elderly Acute Myeloid Leukemia and int-2/High Risk Myelodysplastic Syndrome Patients.

OBJECTIVE: To evaluate the efficacy and safety of standard or low-dose chemotherapy followed by HLA-mismatched allogeneic T-cell infusion (allo-TLI) for the treatment of elderly patients with acute myeloid leukemia (AML) and patients with intermediate-2 to high-risk myelodysplastic syndrome (MDS). METHODS: We carried out a prospective, multicenter, single-arm clinical trial. Totally of 25 patients were enrolled, including 17 AML patients and 8 MDS patients. Each patient received four courses of non-ablative chemotherapy, with HLA-mismatched donor CD3+ allo-TLI 24 h after each course. AML patients received chemotherapy with decitabine, idarubicin, and cytarabine, and MDS patients received decitabine, cytarabine, aclarubicin, and granulocyte colony-stimulating factor. RESULTS: A total of 79 procedures were performed. The overall response rates of the AML and MDS patients were 94% and 75% and the 1-year overall survival rates were 88% (61-97%) and 60% (13-88%), respectively. The overall 60-day treatment-related mortality was 8%. Compared with a historical control cohort that received idarubicin plus cytarabine (3 + 7), the study group showed significantly better overall response (94% vs. 50%, P=0.002) and overall survival rates (the 1-year OS rate was 88% vs. 27%, P=0.014). Post-TLI cytokine-release syndrome (CRS) occurred after 79% of allo-TLI operations, and 96% of CRS reactions were grade 1. CONCLUSION: Elderly AML patients and intermediate-2 to high-risk MDS patients are usually insensitive to or cannot tolerate regular chemotherapies, and may not have the opportunity to undergo allogeneic stem cell transplantation. Our study showed that non-ablative chemotherapy followed by HLA-mismatched allo-TLI is safe and effective, and may thus be used as a first-line treatment for these patients. CLINICAL TRIAL REGISTRATION: https://www.chictr.org.cn/showproj.aspx?proj=20112.

Biosorption of Congo Red from Aqueous Solutions Based on Self-Immobilized Mycelial Pellets: Kinetics, Isotherms, and Thermodynamic Studies.

In the current study, Aspergillus fumigatus and Pseudomonas putida were co-cultured to obtain self-immobilized mycelial pellets to evaluate the decolorization efficiency of Congo red (CR). The obtained co-culture exhibited the highest decolorization efficiency of 99.22% compared to monoculture of A. fumigatus (89.20%) and P. putida (55.04%). The morphology and surface properties of the mycelial pellets were characterized by SEM, FTIR, BET, and XPS. The adsorption kinetics and isotherms were well described by pseudo-second-order and Langmuir models. The findings revealed that the removal efficiency of the mycelial pellet for CR was significantly influenced by physicochemical parameters. Thermodynamic result showed that the biosorption process was endothermic. The maximum adsorption capacity can be obtained from the Langmuir model, which is 316.46 mg/g, it suggests that mycelial pellet was an efficient biosorbent to remove CR from aqueous solution. This study indicates that the mycelial pellet can develop a sustainable approach to eliminate CR from the wastewater.