RESUMEN
BACKGROUND: Tamoxifen (TAM) is a frequently-used treatment for breast cancer (BC). But the TAM resistance seriously affects the patient therapeutic effect. Previous research indicated that circular RNAs (circRNAs) might participate in the regulatory processes of BC. Here, we discovered the parts of circular RNA tripartite motif-containing 28 (circTRIM28) in BC. METHODS: CircTRIM28, microRNA-409-3p (miR-409-3p), and high mobility group AT-hook 2 (HMGA2) levels were perceived by qRT-PCR and western blot. Moreover, the biological functions of the cells were examined. Furthermore, dual-luciferase report was employed to reconnoiter the targeted relationship between miR-409-3p and circTRIM28 or HMGA2. RESULTS: CircTRIM28 and HMGA2 were augmented, and the miR-409-3p was repressed in BC. Silencing circTRIM28 enhanced tamoxifen sensitivity and cell apoptosis, whereas hampered cell development in BC cells. In mechanism, circTRIM28 could sponge miR-409-3p to increase HMGA2. In addition, silencing circTRIM28 impeded tumor growth. CONCLUSION: CircTRIM28 facilitated the BC via miR-409-3p/HMGA2.
Asunto(s)
Neoplasias de la Mama , MicroARNs , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/genética , Neoplasias de la Mama/patología , Línea Celular Tumoral , Movimiento Celular/genética , Proliferación Celular/genética , Femenino , Regulación Neoplásica de la Expresión Génica , Proteína HMGA2/metabolismo , Humanos , MicroARNs/genética , ARN Circular/genética , Tamoxifeno/farmacología , Tamoxifeno/uso terapéuticoRESUMEN
METHODS: In this article, cultivated and wild Rhizoma Polygonati Odorati have been compared in macroscopical identificaation, microscopical identification, contents of odoratan of Rhizoma Polygonati Odorati. RESULTS: Cultivated and wild Rhizoma Polygonati Odorati are different in macroscopical identification, microscopical identification. Contents of odoratan of Rhizoma Polygonati Odorati: wild Rhizoma Polygonati Odorati 9.67%, cultivated Rhizoma Polygonati Odorait 8.05%, wild > cultivated.
Asunto(s)
Plantas Medicinales/anatomía & histología , Polygonatum/anatomía & histología , Polisacáridos/análisis , Epidermis de la Planta/anatomía & histología , Epidermis de la Planta/química , Epidermis de la Planta/citología , Polygonatum/química , Polygonatum/citología , Polvos , Rizoma/anatomía & histología , Rizoma/química , Rizoma/citología , Espectrofotometría UltravioletaRESUMEN
OBJECTIVE: To study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control. METHOD: The ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid (5:4:1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultraviolet light (365 nm). The plate was scanned at wavelengths of lambdaR = 500 nm, lambdaS = 280 nm. RESULT: A fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up. CONCLUSION: The method can be used for quality control of P. odoratum.
Asunto(s)
Cromatografía en Capa Delgada/métodos , Flavonoides/análisis , Plantas Medicinales/química , Polygonatum/química , Flavonoides/química , Control de Calidad , Rizoma/químicaRESUMEN
OBJECTIVE: To observe the effects of lipopolysaccharides of Bacterium prodigiosum (BP-LPS) in inhibiting tumor growth and improving immunosuppression in mice. METHODS: In mice bearing S180 tumor and a mouse model of immunosuppression induced by cyclophosphamide (CTX), the tumor growth, indexes of the immune organs and peripheral white blood cell count were measured after intraperitoneal injection of BP-LPS. RESULTS: Injections of BP-LPS (40 U/kg) for 8 consecutive days resulted in a significant inhibition of the tumor growth in mice bearing S180 tumor (P<0.01), with a dose-dependent increase of the spleen indexes but no obvious changes in the thymus indexes. Intraperitoneal injections of BP-LPS for 7 days inhibited the reduction of peripheral white blood cells and spleen indexes in immunosuppressive mice, but did not produce any significant changes in normal mice. CONCLUSION: BP-LPS can inhibit the tumor growth in tumor-bearing mice and enhance the immune functions of immunosuppressive mice.