RESUMEN
OBJECTIVE: To develop a method of quality control for Hugan qingzhi tablets. METHODS: Fructus Crataegi, Rhizoma Alismatis and Radix Notoginseng were identified by TLC. HPLC was used for the determination of ursolic acid in Hugan qingzhi tablets. RESULTS: The chromatographic spots were identified without the interference of negative control. Ursolic acid had a good linearity over the concentration range of 40-200 microg/mL (r = 1.000). The average recoveries was 99.05% with relatively standard deviations of 1.3%. CONCLUSION: This method is reliable, accurate and specific and can be used for the quality control of Hugan qingzhi tablets.
Asunto(s)
Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/normas , Hipolipemiantes/química , Hipolipemiantes/normas , Plantas Medicinales , Triterpenos/análisis , Alisma/química , Cromatografía Líquida de Alta Presión , Cromatografía en Capa Delgada , Crataegus/química , Combinación de Medicamentos , Medicamentos Herbarios Chinos/administración & dosificación , Hipolipemiantes/administración & dosificación , Panax notoginseng/química , Plantas Medicinales/química , Control de Calidad , Reproducibilidad de los Resultados , Rizoma/química , Comprimidos , Ácido UrsólicoRESUMEN
OBJECTIVE: To study the extraction process for reforming the traditional preparation process of Baohe Wan. METHOD: Using the recovery rate of total flavonoids of Baohe Wan and pharmacodynamic effects as indices, different extraction processes were compared. RESULTS: Supercritical carbon dioxide fluid extraction combined with water extraction was optimal for the extraction, and the extract prepared by the new process was pharmacodynamically more potent than the original Baohe Wan, as demonstrated by the rate of carbon powder moving in the small intestine and stomach emptying rate in mice. CONCLUSION: Supercritical carbon dioxide fluid extraction combined with water extraction is practical for optimizing the preparation of Baohe Wan.
Asunto(s)
Medicamentos Herbarios Chinos , Vaciamiento Gástrico/efectos de los fármacos , Medicina Tradicional China , Tecnología Farmacéutica/métodos , Animales , Medicamentos Herbarios Chinos/farmacología , Femenino , Flavonoides/análisis , Masculino , RatonesRESUMEN
OBJECTIVE: To evaluate the effect of sustained-release alpha-lipoic acid tablets (SRLA) on blood lipid, glucose and insulin levels in hyperlipidemic New Zealand rabbits. METHODS: Twenty-four New Zealand rabbits were randomized into normal diet group, high-fat diet group, and high-fat diet + SRLA (300 mg/tablet) group with corresponding feed. At the beginning and 4 weeks after the feeding, the serum levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), blood glucose, and serum insulin were measured, and insulin sensitivity index (ISI) was calculated. RESULTS: Four weeks after feeding with high-fat diet, the insulin levels was elevated and the ISI lowered in the New Zealand rabbits, indicating successful establishment of the animal model of hyperlipidemia. Compared with the high-fat diet group, the serum levels of TG, TC, LDL-C and insulin were significantly reduced (P<0.05), and the ISI was significantly increased (P<0.05) in high fat diet + SRLA group. But no statistically significant difference was found in the blood glucose among the 3 groups. CONCLUSION: SRLA can significantly correct blood lipid and insulin disorders in hyperlipidemic New Zealand rabbits and prevent the occurrence of insulin resistance and hyperlipidemia.