Spatial frequency shift super-resolution imaging based on quasiperiodic grating and deep learning.

In this study, we propose a pioneering spatially frequency-shifted super-resolution microscopy technique that utilizes the synergy of quasiperiodic gratings and deep learning. First, a quasiperiodic grating capable of converting evanescent waves into propagating waves is designed. The grating is positioned between the object under investigation and the objective lens, and the high-frequency information carried by the evanescent waves in the near-field region of the object is shifted into the detection window and becomes accessible in the far field for imaging. Subsequently, we provide two deep learning models for image and video reconstructions to achieve the reconstruction of static and dynamic samples respectively. Simulation results demonstrate the high feasibility of the proposed method, and both static and dynamic objects with sub-wavelength features can be resolved. The developed method paves the way to the realization of super-resolution imaging by using a traditional bright-field microscope without the need for an extensive optical system design.

Surface plasmon-enhanced dark-field microsphere-assisted microscopy.

We present for the first time a surface plasmon-enhanced dark-field microsphere-assisted microscopy in imaging both low-contrast dielectric objects and metallic ones. We demonstrate, using an Al patch array as the substrate, the resolution and contrast in imaging low-contrast dielectric objects are improved compared to that of the metal plate substrate and a glass slide in dark-field microscopy (DFM). 365-nm-diameter hexagonally arranged SiO nanodots assembled on the three substrates can be resolved, with the contrast varied from 0.23 to 0.96, and the 300-nm-diameter hexagonally close-packed polystyrene nanoparticles can only be discerned on the Al patch array substrate. The resolution can be further improved by using the dark-field microsphere-assisted microscopy, and an Al nanodot array with a nanodot diameter of ∼65 nm and a center-to-center spacing of 125 nm can be just resolved, which cannot be distinguished in a conventional DFM. The focusing effect of the microsphere, as well as the excitation of the surface plasmons, provides evanescent illumination with enhanced local electric field (E-field) on an object. The enhanced local E-field acts as a near-field excitation source to enhance the scattering of the object, resulting in the improvement of imaging resolution.

Microsphere-assisted dark-field microscopy based on a fully immersed low refractive index microsphere.

Here we find that a fully immersed low refractive index SiO2 microsphere (or a microcylinder, a yeast cell) can clearly distinguish a sample with sub-diffraction features in dark-field illumination mode. The resolvable area of the sample by microsphere-assisted microscopy (MAM) is composed of two regions. One region locates below the microsphere, and a virtual image of this part of the sample is formed by the microsphere first and then the virtual image is received by the microscope. The other region is around the edge of the microsphere, and this part of the sample is directly imaged by the microscope. The simulated region of the enhanced electric field on the sample surface formed by the microsphere is consistent with the resolvable region in the experiment. Our studies show that the enhanced electric field on the sample surface generated by the fully immersed microsphere plays an important role in dark-field MAM imaging, and this finding will have a positive effect on exploring novel mechanisms in resolution improvement of MAM.

Super-resolution imaging of plasmonic nanostructures by microsphere-assisted microscopy.

We fabricate both triangularly and circularly shaped Au, Ag, and Cr nanoparticle arrays and observe the imaging properties of these plasmonic nanostructures by BaTiO3 glass (BTG) microsphere-assisted microscopy. We experimentally find that the resolution of triangularly shaped Ag nanoparticle arrays is higher than that of Au and Cr ones, and a gap resolution of ∼λ/7.7 is demonstrated for the circularly shaped Au, Ag, and Cr nanostructures. Numerical simulations show that when a fully immersed BTG microsphere is dispersed on the surface of a plasmonic nanostructure sample, an enhanced electric field is generated in the vicinity of the sample, especially at the gap of the microsphere and the sample, due to the focusing effect of the microsphere and the excitation of localized surface plasmon resonance in the plasmonic nanostructure. The enhanced electric field in Ag nanostructures is significantly stronger than that in Au and Cr ones. Besides, the microsphere collects, amplifies, and propagates the enhanced near-field information to the far field, resulting in the improvement of imaging resolution.

Direct observation Brownian motion of individual nanoparticles in water using microsphere-assisted microscopy.

Observing Brownian motion of nanoscale objects through a traditional optical microscope is still a challenge. Here, we present a method to overcome this challenge by using a traditional optical microscope assisted with a removable microsphere-embedded thin film. The diffusion coefficient of individual unconstrained polystyrene (PS) nanoparticles with a diameter of 300 nm in water is calculated from their respective mean-square displacement versus time curves, and the measured diffusion coefficient shows good agreement with the theoretical Stokes-Einstein one, proving the feasibility of our method. In addition, the experimental results show that the movement of the PS nanoparticles is slowed down near a plane wall, and the diffusion coefficient is consistent with the theoretical constrained diffusion coefficient, which shows that our method can also study the constrained Brownian motion of nanoparticles constrained near a plane wall. Our research results are helpful for the application of microsphere-assisted microscopy in new fields and also provide a new method for nanoparticle tracking.

Pharmacological activation of CB2 receptor protects against ethanol-induced myocardial injury related to RIP1/RIP3/MLKL-mediated necroptosis.

Chronic ethanol abuse can lead to harmful consequences for the heart, resulting in systolic dysfunction, variability in the heart rate, arrhythmia, and cardiac remodelling. However, the precise molecular mechanism responsible for ethanol-induced cardiomyopathy is poorly understood. In this regard, the present study aimed to describe the RIP1/RIP3/MLKL-mediated necroptotic cell death that may be involved in ethanol-induced cardiomyopathy and characterize CBR-mediated effects on the signalling pathway and myocardial injury. We performed an ethanol vapour administration experiment to analyse the effects of ethanol on cardiac structure and function in male C57BL/6J mice. Ethanol induced a significant decline in the cardiac structure and function, as evidenced by a decline in ejection fraction and fractional shortening, and an increase in serum Creatine Kinase levels, myocardial collagen content, and inflammatory reaction. Furthermore, ethanol also upregulated the expression levels of necroptosis-related markers such as p-RIP1, p-RIP3, and p-MLKL in the myocardium. Nec-1 treatment exerted significant cardioprotective effects by salvaging the heart tissue, improving the cardiac function, and mitigating inflammation and necroptosis. In addition, ethanol abuse caused an imbalance in the endocannabinoid system and regulated two cannabinoid receptors (CB1R and CB2R) in the myocardium. Treatment with selective CB2R agonists, JWH-133 or AM1241, markedly improved the cardiac dysfunction and reduced the ethanol-induced necroptosis in the myocardium. Altogether, our data provide evidence that ethanol abuse-induced cardiotoxicity can possibly be attributed to the RIP1/RIP3/MLKL-mediated necroptosis. Moreover, pharmacological activation of CB2R may represent a new cardioprotective strategy against ethanol-induced cardiotoxicity.

Cannabinoid 1 Receptor Antagonists Play a Neuroprotective Role in Chronic Alcoholic Hippocampal Injury Related to Pyroptosis Pathway.

BACKGROUND: Alcohol use disorders affect millions of people worldwide, and there is growing evidence that excessive alcohol intake causes severe damage to the brain of both humans and animals. Numerous studies on chronic alcohol exposure in animal models have identified that many functional impairments are associated with the hippocampus, which is a structure exhibiting substantial vulnerability to alcohol exposure. However, the precise mechanisms that lead to structural and functional impairments of the hippocampus are poorly understood. Herein, we report a novel cell death type, namely pyroptosis, which accounts for alcohol neurotoxicity in mice. METHODS: For this study, we used an in vivo model to induce alcohol-related neurotoxicity in the hippocampus. Adult male C57BL/6 mice were treated with 95% alcohol vapor either alone or in combination with selective cannabinoid receptor antagonists or agonists, and VX765 (Belnacasan), which is a selective caspase-1 inhibitor. RESULTS: Alcohol-induced in vivo pyroptosis occurs because of an increase in the levels of pyroptotic proteins such as nucleotide-binding domain (NOD)-like receptor protein 3 (NLRP3), caspase-1, gasdermin D (GSDMD), and amplified inflammatory response. Our results indicated that VX765 suppressed the expression of caspase-1 and inhibited the maturation of the proinflammatory cytokines interleukin-1ß (IL-1ß) and IL-18. Additionally, chronic alcohol intake created an imbalance in the endocannabinoid system and regulated 2 cannabinoid receptors (CB1R and CB2R) in the hippocampus. Specific antagonists of CB1R (AM251 and AM281) significantly ameliorated alcohol-induced pyroptosis signaling and inactivated the inflammatory response. CONCLUSIONS: Alcohol induces hippocampal pyroptosis, which leads to neurotoxicity, thereby indicating that pyroptosis may be an essential pathway involved in chronic alcohol-induced hippocampal neurotoxicity. Furthermore, cannabinoid receptors are regulated during this process, which suggests promising therapeutic strategies against alcohol-induced neurotoxicity through pharmacologic inhibition of CB1R.

Proteomic Analysis of Brain Regions Reveals Brain Regional Differences and the Involvement of Multiple Keratins in Chronic Alcohol Neurotoxicity.

AIMS: Alcohol abuse has attracted public attention and chronic alcohol exposure can result in irreversible structural changes in the brain. The molecular mechanisms underlying alcohol neurotoxicity are complex, mandating comprehensive mining of spatial protein expression profile. METHODS: In this study, mice models of chronic alcohol intoxication were established after 95% alcohol vapor administration for 30 consecutive days. On Day 30, striatum (the dorsal and ventral striatum) and hippocampus, the two major brain regions responsible for learning and memorizing while being sensitive to alcohol toxicity, were collected. After that, isobaric tags for relative and absolute quantitation -based quantitative proteomic analysis were carried out for further exploration of the novel mechanisms underlying alcohol neurotoxicity. RESULTS: Proteomic results showed that in the striatum, 29 proteins were significantly up-regulated and 17 proteins were significantly down-regulated. In the hippocampus, 72 proteins were significantly up-regulated, while 2 proteins were significantly down-regulated. Analysis of the overlay proteins revealed that a total of 102 proteins were consistently altered (P < 0.05) in both hippocampus and striatum regions, including multiple keratins such as Krt6a, Krt17 and Krt5. Ingenuity pathway analysis revealed that previously reported diseases/biofunctions such as dermatological diseases and developmental disorders were enriched in those proteins. Interestingly, the glucocorticoid receptor (GR) signaling was among the top enriched pathways in both brain regions, while multiple keratins from the GR signaling such as Krt1 and Krt17 exhibited significantly opposite expression patterns in the two brain nuclei. Moreover, there are several other involved pathways significantly differed between the hippocampus and striatum. CONCLUSIONS: Our data revealed brain regional differences upon alcohol consumption and indicated the critical involvement of keratins from GR signaling in alcohol neurotoxicity. The differences in proteomic results between the striatum and hippocampus suggested a necessity of taking into consideration brain regional differences and intertwined signaling pathways rather than merely focusing on single nuclei or molecule during the study of drug-induced neurotoxicity in the future.

Size confinement and origins of two-photon absorption and refraction in CdSe quantum dots.

Tuning a semiconductor nanomaterial with large two-photon absorption cross section in the near-infrared wavelength and investigating the correlation and origins between its size and third-order nonlinear optical properties are very important in possible applications. In this work, CdSe quantum dots (QDs) with various sizes were successfully prepared, and their size-confined third-order nonlinear optical properties were investigated by Z-scan technique with 100 fs laser pulses at 800 nm wavelength. Both the two-photon absorption and nonlinear refraction were enhanced about 8.1 times with size decrease and then weakened to 2 times with further size decrease. QDs with the diameter of 4.9 nm had the largest nonlinear optical susceptibility of 7.8 × 10-12 esu. The effects of photoinduced dipole moment and local electric field were proposed to explain this trend. And the intrinsic dipole moment and defects in CdSe QDs also had an effect on this nonlinear process.

Example of metal-multi-dielectric-metal cooling metamaterial use in engineering thermal radiation.

This paper introduces radiative cooling metamaterial use in engineering thermal radiation based on metal-multi-dielectric-metal (MMDM) structure. The measured average absorption of the MMDM from 15° to 75° in the "atmosphere window" is 0.81, and the absorption range of the structure hardly changes with the incident angles. Without considering solar radiation, at the thermal conductivity of 6.9 W·m-2·K-1, the surface temperature is 10.8°C lower than the ambient temperature (300 K), and the cooling power is 102 W·m-2. Importantly, when the surface temperature is higher than the ambient temperature, this radiative cooler can lower the surface temperature in engineering thermal radiation with a higher thermal conductivity at the same cooling power.

Surrogate analyte-based quantification of main endocannabinoids in whole blood using liquid chromatography-tandem mass spectrometry.

Endocannabinoids (eCBs) are endogenous ligands of the endocannabinoid system that are known to regulate several physiological and behavioral processes. Previous studies have developed methods for the detection of main eCBs including arachidonylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), mostly in serum or plasma. Whole blood is a superior biomaterial for eCBs analysis owing to the nature of the shortened isolation procedure and decreased risk of 2-AG isomerization during preparation. In this study, a surrogate analyte-based liquid chromatography-tandem mass spectrometry assay was developed for the measurement of AEA, 2-AG and its isomer 1-arachidonoylglycerol (1-AG) using a maximum of 100 µL whole blood. Chromatographic separation was achieved using a reverse-phase column and a gradient elution. Detection was performed in selected reaction monitoring mode with an electrospray ionization source. The limits of detection of three eCBs were 0.05-0.1 ng/mL. Good linearity was observed over the concentration range. Intra- and inter-assay accuracy and precision were ≤10.9 and ≤8.7% at four quality control levels. The response factor and parallelism experiment illustrated that the surrogate analytes were suitable for accurate quantification of the main eCBs in whole blood. This surrogate analyte approach was successfully applied to authentic blood samples obtained from alcohol negative drivers and those under the influence of alcohol.

Super-resolution imaging properties of cascaded microsphere lenses.

In this paper, the imaging properties of a cascaded microsphere lens are studied. The cascaded microsphere lens consists of two lenses. A hexagonally close-packed 960-nm-diameter array of polystyrene microspheres is used as the first lens. The second lens is a single silica microsphere with a diameter of about 5 µm. A Blu-ray disc is observed by both the cascaded microsphere lens and the single silica microsphere. Studies reveal that the magnification of the cascaded microsphere lens is about 1.4 times greater than that of the single silica microsphere, while the field of view of the cascaded microsphere lens, which is close to the diameter of the polystyrene microsphere, is decreased. The focal position of the cascaded lens microsphere must be close to the sample in order to observe it.

Reduced distortion in high-index microsphere imaging by partial immersion.

We experimentally demonstrate that pincushion distortion is obvious when using a BaTiO3 glass (BTG) microsphere fully immersed in ethanol or SU-8 2002 resist to image a Blu-ray disk with sub-diffraction features. The distortion is related to the layer thickness of the immersing medium. For a BTG microsphere partially immersed in the SU-8 resist where the SU-8 thickness is around 4/5 the diameter of the microsphere, its distortion decreases dramatically, but it can still clearly resolve the Blu-ray disk. For such a partially immersed microsphere, the calculated position of the photonic nanojet is outside the microsphere and close to the object, indicating the microsphere has a super-resolution imaging property, and the distortion simulated by ZEMAX is decreased.

Influence of the photonic nanojet of microspheres on microsphere imaging.

The imaging properties of BaTiO3 glass (BTG) microspheres in the diameter range of 5-50 µm which are fully immersed in a polydimethylsiloxane layer are experimentally studied. Our experimental results show that for both Blu-ray disc samples and the single-layer hexagonally close-packed microsphere array samples, with the increase of the diameter of BTG microspheres, the range of focal image positions (RFIP) increases linearly. When the diameter of BTG microspheres increases from 5 to 50 µm, the RFIP changes from 4 to 25 µm. For the microsphere array samples, Talbot effect is observed, and both the position of Talbot images and the Talbot distance depend on the diameter of BTG microspheres. Numerical simulations indicate that the length of the photonic nanojet changes from 2.9 to 7.1 µm when the BTG microsphere size increases from 5 to 50 µm, and the calculated RFIP is between 6 and 24 µm. The calculated RFIPs match well with the experimental ones. Our researches reveal that the RFIP depends on the length of the photonic nanojet of the BTG microsphere.

Analysis of aroma components changes in Gannan navel orange at different growth stages by HS-SPME-GC-MS, OAV, and multivariate analysis.

The ripe Gannan navel oranges have an appealing aroma, but few studies have reported the changes of these aromatic substances during the growth of navel oranges. In this study, changes of aroma components in Gannan navel orange from 119 to 245 days after flowering were systematically studied using headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) coupled with multivariate analysis, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). A total of 43 and 54 aroma components were identified in pulp and peel of navel orange, respectively. The odor active value (OAV) results indicated that 14 substances were the key aroma components during the growth of navel orange. Among them, the contribution of linalool, ß-myrcene and limonene were the highest. The multivariate statistical analysis further confirmed that 14 and 18 compounds could be used as key markers to distinguish the pulp and peel at different growth stages, respectively. Results from this study contributed to a better understanding of the dynamic variation and retention of aroma compounds during navel orange growth, and have great potential for industrial application.

Parenting styles and personal belief in a just world among Chinese children and adolescents: gender, living location, and age as moderators.

Introduction: The positive development of the personal belief in a just world (PBJW) plays a vital role in academic achievement and mental health among children and adolescents. Therefore, it is necessary to understand the influencing factors of PBJW better. The association between parenting styles and PBJW has been well established, but whether this association varies among different groups remains an open issue. The present study aimed to examine the strength of the associations between parenting styles and PBJW among Chinese children and adolescents and the role of certain moderators (gender, living location, and age) in these associations. Methods: This study employed hierarchical regression and simple slopes analyses to examine data from the National Children's Study of China. The database includes 24,013 Chinese children and adolescents in grades 49 (M = 12.76 years, SD = 1.73), with 53.50% boys. Results: The results indicated that (1) authoritative parenting was positively correlated with PBJW; (2) both authoritarian and permissive parenting styles were negatively correlated with PBJW; (3) the positive relationship between authoritative parenting and PBJW was more prominent in urban regions; the negative relationship between authoritarian and PBJW was stronger in urban regions; and the negative relationship between permissive parenting and PBJW was more pronounced among girls and older children and adolescents. Discussion: These findings highlight important associations between parenting styles and the development of PBJW among Chinese children and adolescents, and suggest strategies for policy-makers, educators, and parents to improve PBJW for different types of Chinese children and adolescents.

Experimental far-field imaging properties of a ~5-µm diameter spherical lens.

Microscale lenses are mostly used as near-sighted lenses. The far-field imaging properties of a microscale spherical lens, where the lens is spatially separated from the object, are experimentally studied. Our experimental results show that, for a blu-ray disc (an object) whose spacing is 300 nm, the lens can magnify the stripe patterns of the disc when the lens is spatially separated from the object. In the experimentally tested range (0-14 µm), all the magnified images are virtual images. When the distance is increased from 0 to 14 µm the magnification decreases from 1.47× to 1.20× and the field of view increases from 3.8 to 12.2 µm. The image magnification cannot be described by standard geometrical optics.

Gravity-assisted convective assembly of centimeter-sized uniform two-dimensional colloidal crystals.

We have developed an inexpensive, robust, and easily controlled method, a gravity-assisted convective self-assembly method, to fabricate centimeter-sized uniform two-dimensional colloidal crystals. In this method, centimeter-sized two-dimensional colloidal crystals can be formed when the suspension concentration is in the range of 0.32-2.5 wt %. Once the ordering process starts, the formation of two-dimensional colloidal crystals is not affected when the environmental temperature gradually increases from 3 to 10 °C. Experimental results indicate that, in this method, gravity plays an important role in the colloidal crystal formation. The colloidal particles are transported to the edge of the suspension-glass interface, and the extra particles can be eventually moved to the edge of the slide by gravity.

Mimicking bicolor by changing the reflectance of the substrate in a one-dimensional periodic structure.

In nature, some beetles can display bicolor on their elytra. In order to explore the bicolor mechanism, we experimentally studied the optical and structural properties of the Carabus lafossei beetle. We found a multilayer structure in the cuticle of the beetle. Due to the different multilayer thicknesses in different areas, the beetle displayed bicolor. Here, we provide another approach to fabricate bicolor by depositing the same multilayer stack on a substrate with different reflectances at different areas. In this paper, the substrate with different reflectances is achieved by prefabricating sculpted hexagons (SU-8) on a silicon substrate. By coating a (ZnS/MgF2)3.5 multilayer, the sculpted structure displays green color at the ridges (SU-8/silicon area) and yellow color at the basins (silicon area).

Simultaneous Quantification of 38 Psychotropic Drugs and Relevant Metabolites in Blood using LC-MS-MS.

The trend for the concomitant prescription of antidepressants and antipsychotics is increasing. This calls for a veracious screening and quantifying method for forensic and clinical use. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the simultaneous determination and quantification of 38 antidepressants, antipsychotics and relevant metabolites in small volumes (200 µL) of human whole blood. Analytes and deuterated internal standards were extracted using liquid-liquid extraction. The separation, determination and quantification of the analytes were performed using an LC-MS-MS system equipped with an ACQUITY UPLC® BEH Phenyl Column under a positive electrospray ionization mode. After validation, the analytical procedure was proved to be highly sensitive, with a limit of detection ranging from 0.0005 to 1 ng/mL and a lower limit of quantification ranging from 0.002 to 2 ng/mL. Bias and within- and between-run precision were within 14.7% for all analytes. Recoveries were reproducible and those of 35 analytes were >50%. Dilution integrity was evaluated to ensure that the therapeutic and toxic blood concentration ranges of target compounds were fully covered. Finally, this method was applied to authentic whole blood samples collected from two forensic cases, which demonstrated its practical usefulness of providing accurate and comprehensive information concerning the previous medication of the deceased.