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Although droplet-based microfluidics has been broadly used as a versatile tool in biology, chemistry, and nanotechnology, its rather complicated microfabrication process and the requirement of specialized hardware and operating skills hinder researchers fully unleashing the potential of this powerful platform. Here, we develop an integrated microdroplet generator enabled by a spinning conical frustum for the versatile production of near-monodisperse microdroplets in a high-throughput and off-chip manner. The construction and operation of this generator are simple and straightforward without the need of microfabrication, and we demonstrate that the generator is able to passively and actively control the size of the produced microdroplets. In addition to water microdroplets, this generator can produce microdroplets of liquid metal that would be difficult to produce in conventional microfluidic platforms as liquid metal has high surface tension. Moreover, we demonstrate that this generator can produce solid hydrogel microparticles and fibers using integrated ultraviolet (UV) light. In the end, we further explore the ability of this generator for forming double emulsions by coflowing two immiscible liquids. Given the remarkable abilities demonstrated by this platform and the tremendous potential of microdroplets, this user-friendly method may revolutionize the future of droplet-based chemical synthesis and biological analysis.
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Técnicas Analíticas Microfluídicas/métodos , Emulsiones/análisis , Hidrogeles/análisis , Metales/análisis , Técnicas Analíticas Microfluídicas/instrumentación , Microfluídica/métodos , Microtecnología , Tamaño de la Partícula , Tensión Superficial , Rayos Ultravioleta , Agua/químicaRESUMEN
Functional nanoparticles comprised of liquid metals, such as eutectic gallium indium (EGaIn) and Galinstan, present exciting opportunities in the fields of flexible electronics, sensors, catalysts, and drug delivery systems. Methods used currently for producing liquid metal nanoparticles have significant disadvantages as they rely on both bulky and expensive high-power sonication probe systems, and also generally require the use of small molecules bearing thiol groups to stabilize the nanoparticles. Herein, an innovative microfluidics-enabled platform is described as an inexpensive, easily accessible method for the on-chip mass production of EGaIn nanoparticles with tunable size distributions in an aqueous medium. A novel nanoparticle-stabilization approach is reported using brushed polyethylene glycol chains with trithiocarbonate end-groups negating the requirements for thiol additives while imparting a "stealth" surface layer. Furthermore, a surface modification of the nanoparticles is demonstrated using galvanic replacement and conjugation with antibodies. It is envisioned that the demonstrated microfluidic technique can be used as an economic and versatile platform for the rapid production of liquid metal-based nanoparticles for a range of biomedical applications.
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Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point-of-care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean-room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample-reagent mixing. With the integration of smartphone-based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone-based detection platform is portable for on-site quantitative detection.
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Técnicas Analíticas Microfluídicas/métodos , Proteinuria/orina , Teléfono Inteligente/instrumentación , Estereolitografía , Colorimetría/métodos , Diseño de Equipo/instrumentación , Humanos , Imagen Óptica/instrumentación , Procesos Fotoquímicos , Polimetil Metacrilato/química , Albúmina Sérica Bovina/química , Propiedades de SuperficieRESUMEN
This work presents a simple, low-cost method to fabricate semi-circular channels using solder paste, which can amalgamate the cooper surface to form a half-cylinder mold using the surface tension of Sn-Pd alloy (the main component in solder paste). This technique enables semi-circular channels to be manufactured with different dimensions. These semi-circular channels will then be integrated with a polymethylmethacrylate frame and machine screws to create miniaturized, portable microfluidic valves for sequential liquid delivery and particle synthesis. This approach avoids complicated fabrication processes and expensive facilities and thus has the potential to be a useful tool for lab-on-a-chip applications.
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Microfluídica , Aleaciones/química , Diseño de Equipo/instrumentación , Dispositivos Laboratorio en un Chip , Paladio/química , Tensión Superficial , Estaño/químicaRESUMEN
In this work, a novel double-layer microfluidic device for enhancing particle focusing was presented. The double-layer device consists of a channel with expansion-contraction array and periodical slanted grooves. The secondary flows induced by the grooves modulate the flow patterns in the expansion-contraction-array (ECA) channel, further affecting the particle migration. Compared with the single ECA channel, the double-layer channel can focus the particles over a wider range of flow rate. Due to the differentiation of lateral migration, the double-layer channel is able to distinguish the particles with different sizes. Furthermore, the equilibrium positions could be modulated by the orientation of grooves. This work demonstrates the possibility to enhance and adjust the inertial focusing in an ECA channel with the assistance of grooves, which may provide a simple and portable platform for downstream filtration, separation, and detection.
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Dispositivos Laboratorio en un Chip , Tamaño de la PartículaRESUMEN
Prosthetic hands play a vital role in restoring forearm functionality for patients who have suffered hand loss or deformity. The hand gesture intention recognition system serves as a critical component within the prosthetic hand system. However, accurately and swiftly identifying hand gesture intentions remains a challenge in existing approaches. Here, a real-time motion intention recognition system utilizing liquid metal composite sensor bracelets is proposed. The sensor bracelet detects pressure signals generated by forearm muscle movements to recognize hand gesture intent. Leveraging the remarkable pressure sensitivity of liquid metal composites and the efficient classifier based on the optimized recognition algorithm, this system achieves an average offline and real-time recognition accuracy of 98.2% and 92.04%, respectively, with an average recognition speed of 0.364 s. Thus, this wearable system shows advantages in superior recognition speed and accuracy. Furthermore, this system finds applications in master-slave control of prosthetic hands in unmanned scenarios, such as electrically powered operations, space exploration, and telemedicine. The proposed system promises significant advances in next-generation intent-controlled prosthetic hands and robots.
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Materials following Murray's law are of significant interest due to their unique porous structure and optimal mass transfer ability. However, it is challenging to construct such biomimetic hierarchical channels with perfectly cylindrical pores in synthetic systems following the existing theory. Achieving superior mass transport capacity revealed by Murray's law in nanostructured materials has thus far remained out of reach. We propose a Universal Murray's law applicable to a wide range of hierarchical structures, shapes and generalised transfer processes. We experimentally demonstrate optimal flow of various fluids in hierarchically planar and tubular graphene aerogel structures to validate the proposed law. By adjusting the macroscopic pores in such aerogel-based gas sensors, we also show a significantly improved sensor response dynamics. In this work, we provide a solid framework for designing synthetic Murray materials with arbitrarily shaped channels for superior mass transfer capabilities, with future implications in catalysis, sensing and energy applications.
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Materials with programmable conductivity and stiffness offer new design opportunities for next-generation engineered systems in soft robotics and electronic devices. However, existing approaches fail to harness variable electrical and mechanical properties synergistically and lack the ability to self-respond to environmental changes. We report an electro-mechano responsive Field's metal hybrid elastomer exhibiting variable and tunable conductivity, strain sensitivity, and stiffness. By synergistically harnessing these properties, we demonstrate two applications with over an order of magnitude performance improvement compared to state-of-the-art, including a self-triggered multiaxis compliance compensator for robotic manipulators, and a resettable, highly compact, and fast current-limiting fuse with an adjustable fusing current. We envisage that the extraordinary electromechanical properties of our hybrid elastomer will bring substantial advancements in resilient robotic systems, intelligent instruments, and flexible electronics.
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Flexible pressure sensors based on polymer elastomers filled with conductive fillers show great advantages in their applications in flexible electronic devices. However, integratable high-sensitivity pressure sensors remain understudied. This work improves the conductivity and sensitivity of PDMS-Fe/Ni piezoresistive composites by introducing silver flakes and magnetic-assisted alignment techniques. As secondary fillers, silver flakes with high aspect ratios enhance the conductive percolation network in composites. Meanwhile, a magnetic field aligns ferromagnetic particles to further improve the conductivity and sensitivity of composites. The resistivity of the composite decreases sharply by 1000 times within a tiny compression strain of 1%, indicating excellent sensing performance. On the basis of this, we demonstrate an integratable miniature pressure sensor with a small size (2 × 2 × 1 mm), high sensitivity (0.966 kPa-1), and wide sensing range (200 kPa). Finally, we develop a flexible E-skin system with 5 × 5 integratable sensor units to detect pressure distribution, which shows rapid real-time response, high resolution, and high sensitivity.
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Portability and low-cost analytic ability are desirable for point-of-care (POC) diagnostics; however, current POC testing platforms often require time-consuming multiple microfabrication steps and rely on bulky and costly equipment. This hinders the capability of microfluidics to prove its power outside of laboratories and narrows the range of applications. This paper details a self-contained microfluidic device, which does not require any external connection or tubing to deliver insert-and-use image-based analysis. Without any microfabrication, magnetorheological elastomer (MRE) microactuators including pumps, mixers and valves are integrated into one modular microfluidic chip based on novel manipulation principles. By inserting the chip into the driving and controlling platform, the system demonstrates sample preparation and sequential pumping processes. Furthermore, due to the straightforward fabrication process, chips can be rapidly reconfigured at a low cost, which validates the robustness and versatility of an MRE-enabled microfluidic platform as an option for developing an integrated lab-on-a-chip system.
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Correction for 'Modular off-chip emulsion generator enabled by a revolving needle' by Yuxin Zhang et al., Lab Chip, 2020, 20, 4592-4599, DOI: 10.1039/D0LC00939C.
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Soft crawling robots have potential applications for surveillance, rescue, and detection in complex environments. Despite this, most existing soft crawling robots either use nonadjustable feet to passively induce asymmetry in friction to actuate or are only capable of moving on surfaces with specific designs. Thus, robots often lack the ability to move along arbitrary directions in a two-dimensional (2D) plane or in unpredictable environments such as wet surfaces. Here, leveraging the electrochemically tunable interfaces of liquid metal, we report the development of liquid metal smart feet (LMSF) that enable electrical control of friction for achieving versatile actuation of prismatic crawling robots on wet slippery surfaces. The functionality of the LMSF is examined on crawling robots with soft or rigid actuators. Parameters that affect the performance of the LMSF are investigated. The robots with the LMSF prove capable of actuating across different surfaces in various solutions. Demonstration of 2D locomotion of crawling robots along arbitrary directions validates the versatility and reliability of the LMSF, suggesting broad utility in the development of advanced soft robotic systems.
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Microfluidic chips have demonstrated unparalleled abilities in droplet generation, including precise control over droplet size and monodispersity. And yet, their rather complicated microfabrication process and operation can be a barrier for inexperienced researchers, which hinders microdroplets from unleashing their potential in broader fields of research. Here, we attempt to remove this barrier by developing an integrated and modular revolving needle emulsion generator (RNEG) to achieve high-throughput production of uniformly sized droplets in an off-chip manner. The RNEG works by driving a revolving needle to pinch the dispersed phase in a minicentrifuge tube. The system is constructed using modular components without involving any microfabrication, thereby enabling user-friendly operation. The RNEG is capable of producing microdroplets of various liquids with diameters ranging from tens to hundreds of micrometres. We further examine the principle of operation using numerical simulations and establish a simple model to predict the droplet size. Moreover, by integrating curing and centrifugation processes, the RNEG can produce hydrogel microparticles and transfer them from an oil phase into a water phase. Using this ability, we demonstrate the encapsulation and culture of single yeast cells within hydrogel microparticles. We envisage that the RNEG can become a versatile and powerful tool for high-throughput production of emulsions to facilitate diverse biological and chemical research.
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Nanoparticles (NPs) and microparticles (MPs) have been widely used in different areas of research such as materials science, energy, and biotechnology. On-demand synthesis of NPs and MPs with desired chemical and physical properties is essential for different applications. However, most of the conventional methods for producing NPs/MPs require bulky and expensive equipment, which occupies large space and generally need complex operation with dedicated expertise and labour. These limitations hinder inexperienced researchers to harness the advantages of NPs and MPs in their fields of research. When problems individual researchers accumulate, the overall interdisciplinary innovations for unleashing a wider range of directions are undermined. In recent years, modular and integrated systems are developed for resolving the ongoing dilemma. In this review, we focus on the development of modular and integrated systems that assist the production of NPs and MPs. We categorise these systems into two major groups: systems for the synthesis of (1) NPs and (2) MPs; systems for producing NPs are further divided into two sections based on top-down and bottom-up approaches. The mechanisms of each synthesis method are explained, and the properties of produced NPs/MPs are compared. Finally, we discuss existing challenges and outline the potentials for the development of modular and integrated systems.
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Química Física , Nanopartículas/químicaRESUMEN
Conductive elastic composites have been used widely in soft electronics and soft robotics. These composites are typically a mixture of conductive fillers within elastomeric substrates. They can sense strain via changes in resistance resulting from separation of the fillers during elongation. Thus, most elastic composites exhibit a negative piezoconductive effect, i.e. the conductivity decreases under tensile strain. This property is undesirable for stretchable conductors since such composites may become less conductive during deformation. Here, we report a liquid metal-filled magnetorheological elastomer comprising a hybrid of fillers of liquid metal microdroplets and metallic magnetic microparticles. The composite's resistivity reaches a maximum value in the relaxed state and drops drastically under any deformation, indicating that the composite exhibits an unconventional positive piezoconductive effect. We further investigate the magnetic field-responsive thermal properties of the composite and demonstrate several proof-of-concept applications. This composite has prospective applications in sensors, stretchable conductors, and responsive thermal interfaces.
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Microalgae cells have been recognized as a promising sustainable resource to meet worldwide growing demands for renewable energy, food, livestock feed, water, cosmetics, pharmaceuticals, and materials. In order to ensure high-efficiency and high-quality production of biomass, biofuel, or bio-based products, purification procedures prior to the storage and cultivation of the microalgae from contaminated bacteria are of great importance. The present work proposed and developed a simple, sheathless, and efficient method to separate microalgae Chlorella from bacteria Bacillus Subtilis in a straight channel using the viscoelasticity of the medium. Microalgae and bacteria migrate to different lateral positions closer to the channel centre and channel walls respectively. Fluorescent microparticles with 1 µm and 5 µm diameters were first used to mimic the behaviours of bacteria and microalgae to optimize the separating conditions. Subsequently, size-based separation in Newtonian fluid and in viscoelastic fluid in straight channels with different aspect ratios was compared and demonstrated. Under the optimal condition, the removal ratio for 1 µm microparticles and separation efficiency for 5 µm particles can reach up to 98.28% and 93.85% respectively. For bacteria and microalgae cells separation, the removal ratio for bacteria and separation efficiency for microalgae cells is 92.69% and 100% respectively. This work demonstrated the continuous and sheathless separation of microalgae from bacteria for the first time by viscoelastic microfluidics. This technique can also be applied as an efficient and user-friendly method to separate mammalian cells or other kinds of cells.
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Bacillus subtilis/química , Microalgas/química , Técnicas Analíticas Microfluídicas , Sustancias Viscoelásticas/química , Microalgas/citología , Técnicas Analíticas Microfluídicas/instrumentación , Tamaño de la PartículaRESUMEN
Numerous lab-on-a-chip applications benefit from channels with complex structures and configurations in the areas of tissue engineering and clinical diagnostics. The current fabrication approaches require time-consuming, complicated processes and bulky, expensive facilities. In this work, we propose a novel method for the fabrication of complex channels with the assistance of amalgamation of liquid metal with copper tape. This new technique enables the rapid fabrication of liquid metal molds with various dimensions and diverse structures. Two proof-of-concept experiments were conducted to verify the utilization of this method. First, the channel replicated from the liquid metal mold is used to enhance the mixing performance of liquids flowing through the channel. Second, a channel with a semicircular cross-section is fabricated to achieve 3D focusing in a simple way. This proposed technique can be readily used for fabricating complex channels for a wide range of applications.