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1.
Nature ; 593(7859): 435-439, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33953403

RESUMEN

Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and apoptosis1-3. Dysregulation has been linked to neurodegeneration3,4, cardiovascular disease3 and cancer5. Key components of the fission machinery include the endoplasmic reticulum6 and actin7, which initiate constriction before dynamin-related protein 1 (DRP1)8 binds to the outer mitochondrial membrane via adaptor proteins9-11, to drive scission12. In the mitochondrial life cycle, fission enables both biogenesis of new mitochondria and clearance of dysfunctional mitochondria through mitophagy1,13. Current models of fission regulation cannot explain how those dual fates are decided. However, uncovering fate determinants is challenging, as fission is unpredictable, and mitochondrial morphology is heterogeneous, with ultrastructural features that are below the diffraction limit. Here, we used live-cell structured illumination microscopy to capture mitochondrial dynamics. By analysing hundreds of fissions in African green monkey Cos-7 cells and mouse cardiomyocytes, we discovered two functionally and mechanistically distinct types of fission. Division at the periphery enables damaged material to be shed into smaller mitochondria destined for mitophagy, whereas division at the midzone leads to the proliferation of mitochondria. Both types are mediated by DRP1, but endoplasmic reticulum- and actin-mediated pre-constriction and the adaptor MFF govern only midzone fission. Peripheral fission is preceded by lysosomal contact and is regulated by the mitochondrial outer membrane protein FIS1. These distinct molecular mechanisms explain how cells independently regulate fission, leading to distinct mitochondrial fates.


Asunto(s)
Mitocondrias/metabolismo , Dinámicas Mitocondriales , Mitofagia , Actinas , Animales , Células COS , Supervivencia Celular , Células Cultivadas , Chlorocebus aethiops , ADN Mitocondrial/análisis , ADN Mitocondrial/metabolismo , Dinaminas , Retículo Endoplásmico , Humanos , Lisosomas , Proteínas de la Membrana , Ratones , Mitocondrias/genética , Proteínas Mitocondriales
2.
Nucleic Acids Res ; 45(10): 6135-6146, 2017 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-28335001

RESUMEN

FASTK family proteins have been identified as regulators of mitochondrial RNA homeostasis linked to mitochondrial diseases, but much remains unknown about these proteins. We show that CRISPR-mediated disruption of FASTKD1 increases ND3 mRNA level, while disruption of FASTKD4 reduces the level of ND3 and of other mature mRNAs including ND5 and CYB, and causes accumulation of ND5-CYB precursor RNA. Disrupting both FASTKD1 and FASTKD4 in the same cell results in decreased ND3 mRNA similar to the effect of depleting FASTKD4 alone, indicating that FASTKD4 loss is epistatic. Interestingly, very low levels of FASTKD4 are sufficient to prevent ND3 loss and ND5-CYB precursor accumulation, suggesting that FASTKD4 may act catalytically. Furthermore, structural modeling predicts that each RAP domain of FASTK proteins contains a nuclease fold with a conserved aspartate residue at the putative active site. Accordingly, mutation of this residue in FASTKD4 abolishes its function. Experiments with FASTK chimeras indicate that the RAP domain is essential for the function of the FASTK proteins, while the region upstream determines RNA targeting and protein localization. In conclusion, this paper identifies new aspects of FASTK protein biology and suggests that the RAP domain function depends on an intrinsic nucleolytic activity.


Asunto(s)
Citocromos b/genética , Complejo I de Transporte de Electrón/genética , Mitocondrias/metabolismo , Proteínas Mitocondriales/genética , Proteínas Mitocondriales/fisiología , ARN Mensajero/metabolismo , Proteínas de Unión al ARN/fisiología , ARN/metabolismo , Secuencia de Aminoácidos , Sistemas CRISPR-Cas , Regulación de la Expresión Génica , Técnicas de Silenciamiento del Gen , Células HEK293 , Humanos , Mitocondrias/ultraestructura , Proteínas Mitocondriales/química , Modelos Moleculares , Conformación Proteica , Dominios Proteicos , ARN/genética , ARN Mensajero/genética , ARN Mitocondrial , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/genética , Alineación de Secuencia , Homología de Secuencia , Transcripción Genética
3.
J Biol Chem ; 292(11): 4519-4532, 2017 03 17.
Artículo en Inglés | MEDLINE | ID: mdl-28082677

RESUMEN

Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized into distinct foci, which we have previously termed mitochondrial RNA granules (MRGs). Although an increasing number of proteins have been localized to MRGs, a comprehensive analysis of the proteome of these structures is still lacking. Here, we have applied a microscopy-based approach that has allowed us to identify novel components of the MRG proteome. Among these, we have focused our attention on RPUSD4, an uncharacterized mitochondrial putative pseudouridine synthase. We show that RPUSD4 depletion leads to a severe reduction of the steady-state level of the 16S mitochondrial (mt) rRNA with defects in the biogenesis of the mitoribosome large subunit and consequently in mitochondrial translation. We report that RPUSD4 binds 16S mt-rRNA, mt-tRNAMet, and mt-tRNAPhe, and we demonstrate that it is responsible for pseudouridylation of the latter. These data provide new insights into the relevance of RNA pseudouridylation in mitochondrial gene expression.


Asunto(s)
Transferasas Intramoleculares/metabolismo , ARN/metabolismo , Línea Celular , Humanos , Transferasas Intramoleculares/análisis , Transferasas Intramoleculares/genética , Mitocondrias/genética , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Transporte de Proteínas , Interferencia de ARN , ARN Mitocondrial , ARN Ribosómico 16S/metabolismo , ARN Interferente Pequeño/genética , ARN de Transferencia de Metionina/metabolismo , ARN de Transferencia de Fenilalanina/metabolismo
4.
Nat Cell Biol ; 22(10): 1180-1186, 2020 10.
Artículo en Inglés | MEDLINE | ID: mdl-32989247

RESUMEN

Mitochondria contain the genetic information and expression machinery to produce essential respiratory chain proteins. Within the mitochondrial matrix, newly synthesized RNA, RNA processing proteins and mitoribosome assembly factors form punctate sub-compartments referred to as mitochondrial RNA granules (MRGs)1-3. Despite their proposed importance in regulating gene expression, the structural and dynamic properties of MRGs remain largely unknown. We investigated the internal architecture of MRGs using fluorescence super-resolution localization microscopy and correlative electron microscopy, and found that the MRG ultrastructure consists of compacted RNA embedded within a protein cloud. Using live-cell super-resolution structured illumination microscopy and fluorescence recovery after photobleaching, we reveal that MRGs rapidly exchange components and can undergo fusion, characteristic properties of fluid condensates4. Furthermore, MRGs associate with the inner mitochondrial membrane and their fusion coincides with mitochondrial remodelling. Inhibition of mitochondrial fission or fusion leads to an aberrant accumulation of MRGs into concentrated pockets, where they remain as distinct individual units despite their close apposition. Together, our findings reveal that MRGs are nanoscale fluid compartments, which are dispersed along mitochondria via membrane dynamics.


Asunto(s)
Mitocondrias/fisiología , Dinámicas Mitocondriales , Membranas Mitocondriales/fisiología , Proteínas Mitocondriales/metabolismo , Ribosomas Mitocondriales/fisiología , ARN Mitocondrial/metabolismo , Proteínas de Unión al ARN/metabolismo , Células HeLa , Humanos , Microscopía Fluorescente , Proteínas Mitocondriales/genética , ARN Mitocondrial/genética , Proteínas de Unión al ARN/genética
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