RESUMEN
Dendritic cells (DCs) phagocytose large particles like bacteria at sites of infection and progressively degrade them within maturing phagosomes. Phagosomes in DCs are also signaling platforms for pattern recognition receptors, such as Toll-like receptors (TLRs), and sites for assembly of cargo-derived peptides with major histocompatibility complex class II (MHC-II) molecules. Although TLR signaling from phagosomes stimulates presentation of phagocytosed antigens, the mechanisms underlying this enhancement and the cell surface delivery of MHC-II-peptide complexes from phagosomes are not known. We show that in DCs, maturing phagosomes extend numerous long tubules several hours after phagocytosis. Tubule formation requires an intact microtubule and actin cytoskeleton and MyD88-dependent phagosomal TLR signaling, but not phagolysosome formation or extensive proteolysis. In contrast to the tubules that emerge from endolysosomes after uptake of soluble ligands and TLR stimulation, the late-onset phagosomal tubules are not essential for delivery of phagosome-derived MHC-II-peptide complexes to the plasma membrane. Rather, tubulation promotes MHC-II presentation by enabling maximal cargo transfer among phagosomes that bear a TLR signature. Our data show that phagosomal tubules in DCs are functionally distinct from those that emerge from lysosomes and are unique adaptations of the phagocytic machinery that facilitate cargo exchange and antigen presentation among TLR-signaling phagosomes.
Asunto(s)
Presentación de Antígeno/inmunología , Células Dendríticas/inmunología , Antígenos de Histocompatibilidad Clase II/inmunología , Fagosomas/inmunología , Receptores Toll-Like/metabolismo , Actinas/metabolismo , Animales , Lipopolisacáridos , Lisosomas/metabolismo , Ratones Endogámicos C57BL , Microtúbulos/metabolismo , Factor 88 de Diferenciación Mieloide/metabolismo , Péptidos/metabolismo , Transducción de Señal , Receptor Toll-Like 4/metabolismoRESUMEN
The Drosophila egg chamber is a powerful system to study epithelial cell collective migration and polarized basement membrane secretion. A strength of this system is the ability to capture these dynamic processes in ex vivo organ culture using high-resolution live imaging. Ex vivo culture also allows acute pharmacological or labeling treatments, extending the versatility of the system. However, many current ex vivo egg chamber culture setups do not permit easy medium exchange, preventing researchers from following individual egg chambers through multiple treatments. Here we present a method to immobilize egg chambers in an easy-to-construct flow chamber that permits imaging of the same egg chamber through repeated solution exchanges. This will allow researchers to take greater advantage of the wide variety of available pharmacological perturbations and other treatments like dyes to study dynamic processes in the egg chamber.
Asunto(s)
Proteínas de Drosophila , Drosophila , Animales , Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Movimiento Celular , Diagnóstico por Imagen , Membrana Basal/metabolismo , Proteínas de Drosophila/metabolismo , OogénesisRESUMEN
Epithelial tissues are lined with a sheet-like basement membrane (BM) extracellular matrix at their basal surfaces that plays essential roles in adhesion and signaling. BMs also provide mechanical support to guide morphogenesis. Despite their importance, we know little about how epithelial cells secrete and assemble BMs during development. BM proteins are sorted into a basolateral secretory pathway distinct from other basolateral proteins. Because BM proteins self-assemble into networks, and the BM lines only a small portion of the basolateral domain, we hypothesized that the site of BM protein secretion might be tightly controlled. Using the Drosophila follicular epithelium, we show that kinesin-3 and kinesin-1 motors work together to define this secretion site. Similar to all epithelia, the follicle cells have polarized microtubules (MTs) along their apical-basal axes. These cells collectively migrate, and they also have polarized MTs along the migratory axis at their basal surfaces. We find follicle cell MTs form one interconnected network, which allows kinesins to transport Rab10+ BM secretory vesicles both basally and to the trailing edge of each cell. This positions them near the basal surface and the basal-most region of the lateral domain for exocytosis. When kinesin transport is disrupted, the site of BM protein secretion is expanded, and ectopic BM networks form between cells that impede migration and disrupt tissue architecture. These results show how epithelial cells can define a subdomain on their basolateral surface through MT-based transport and highlight the importance of controlling the exocytic site of network-forming proteins.
Asunto(s)
Proteínas de Drosophila , Drosophila , Animales , Membrana Basal/metabolismo , Drosophila/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Células Epiteliales/metabolismo , Cinesinas , Proteínas de la Membrana/metabolismoRESUMEN
Membrane traffic can be studied by imaging a cargo protein as it transits the secretory pathway. The best tools for this purpose initially block export of the secretory cargo from the endoplasmic reticulum (ER) and then release the block to generate a cargo wave. However, previously developed regulatable secretory cargoes are often tricky to use or specific for a single model organism. To overcome these hurdles for budding yeast, we recently optimized an artificial fluorescent secretory protein that exits the ER with the aid of the Erv29 cargo receptor, which is homologous to mammalian Surf4. The fluorescent secretory protein forms aggregates in the ER lumen and can be rapidly disaggregated by addition of a ligand to generate a nearly synchronized cargo wave. Here we term this regulatable secretory protein ESCargo (Erv29/Surf4-dependent secretory cargo) and demonstrate its utility not only in yeast cells, but also in cultured mammalian cells, Drosophila cells, and the ciliate Tetrahymena thermophila. Kinetic studies indicate that rapid export from the ER requires recognition by Erv29/Surf4. By choosing an appropriate ER signal sequence and expression vector, this simple technology can likely be used with many model organisms.
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Modelos Biológicos , Proteínas/metabolismo , Vías Secretoras , Animales , Drosophila melanogaster/citología , Drosophila melanogaster/metabolismo , Retículo Endoplásmico/metabolismo , Humanos , Neuronas/metabolismo , Transporte de Proteínas , Ratas , Saccharomyces cerevisiae/metabolismo , Tetrahymena/metabolismoRESUMEN
Collective migration of epithelial cells is essential for morphogenesis, wound repair, and the spread of many cancers, yet how individual cells signal to one another to coordinate their movements is largely unknown. Here, we introduce a tissue-autonomous paradigm for semaphorin-based regulation of collective cell migration. Semaphorins typically regulate the motility of neuronal growth cones and other migrating cell types by acting as repulsive cues within the migratory environment. Studying the follicular epithelial cells of Drosophila, we discovered that the transmembrane semaphorin, Sema-5c, promotes collective cell migration by acting within the migrating cells themselves, not the surrounding environment. Sema-5c is planar polarized at the basal epithelial surface such that it is enriched at the leading edge of each cell. This location places it in a prime position to send a repulsive signal to the trailing edge of the cell ahead to communicate directional information between neighboring cells. Our data show that Sema-5c can signal across cell-cell boundaries to suppress protrusions in neighboring cells and that Plexin A is the receptor that transduces this signal. Finally, we present evidence that Sema-5c antagonizes the activity of Lar, another transmembrane guidance cue that operates along leading-trailing cell-cell interfaces in this tissue, via a mechanism that appears to be independent of Plexin A. Together, our results suggest that multiple transmembrane guidance cues can be deployed in a planar-polarized manner across an epithelium and work in concert to coordinate individual cell movements for collective migration.
Asunto(s)
Movimiento Celular/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/fisiología , Células Epiteliales/fisiología , Glicoproteínas de Membrana/genética , Proteínas del Tejido Nervioso/genética , Proteínas Tirosina Fosfatasas Similares a Receptores/genética , Receptores de Superficie Celular/genética , Semaforinas/genética , Animales , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Glicoproteínas de Membrana/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Proteínas Tirosina Fosfatasas Similares a Receptores/metabolismo , Receptores de Superficie Celular/metabolismo , Semaforinas/metabolismoRESUMEN
A new study upends the clear distinction between cell-cell adhesion and cell-matrix adhesion by showing that type IV collagen is essential for inter-adipocyte adhesion in the Drosophila fat body.
Asunto(s)
Colágeno Tipo IV , Drosophila , Adipocitos/citología , Animales , Adhesión Celular , Transducción de SeñalRESUMEN
The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure-function analysis support this hypothesis. Disruption of the Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment. This behavior leads to randomly inherited centrosomes after neuroblast division. We further show that spindle polarity is maintained in neuroblasts despite centrosome detachment, with the poles remaining stably associated with the cell cortex. Finally, we provide evidence that CaM is required for Asp's spindle function; however, it is completely dispensable for Asp's role in microcephaly suppression.
Asunto(s)
Calmodulina/metabolismo , Centrosoma/metabolismo , Proteínas de Drosophila/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Células-Madre Neurales/citología , Animales , Encéfalo/patología , División Celular , Línea Celular , Polaridad Celular , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas , Drosophila melanogaster/metabolismo , Exones , Genotipo , Proteínas Fluorescentes Verdes/metabolismo , Microtúbulos/metabolismo , Mutación , Fenotipo , Unión Proteica , Interferencia de ARN , Huso Acromático/metabolismoRESUMEN
Nucleation-promoting factors (NPFs) control the spatio-temporal activity of Arp2/3 complex in cells]. Thus, WASP and the WAVE complex direct the formation of branched actin networks at the leading edge during cell motility and endo/exocytosis, whereas the WASH complex is involved in endosomal transport. Less understood are WHAMM and JMY, two NPFs with similar domain architecture. JMY is found in the nucleus and the cytosol and is involved in transcriptional regulation, cell motility, and trans-Golgi transport. WHAMM was reported to bind microtubules and to be involved in ER to cis-Golgi transport. Here, we show that WHAMM directs the activity of Arp2/3 complex for autophagosome biogenesis through an actin-comet tail motility mechanism. Macroautophagy--the process by which cytosolic material is engulfed into autophagosomes for degradation and/or recycling--was recently shown to involve actin, but the mechanism is unknown. We found that WHAMM forms puncta that colocalize and comigrate with the autophagy markers LC3, DFCP1, and p62 through a WHAMM-dependent actin-comet tail mechanism. Under starvation, WHAMM and actin are observed at the interface between neighboring autophagosomes, whose number and size increase with WHAMM expression. Interfering with actin polymerization, inhibiting Arp2/3 complex, knocking down WHAMM, or blocking its interaction with Arp2/3 complex through mutagenesis all inhibit comet tail formation and reduce the size and number of autophagosomes. Finally, JMY shows similar localization to WHAMM and could be involved in similar processes. These results reveal a link between Arp2/3-complex-dependent actin assembly and autophagy.
Asunto(s)
Complejo 2-3 Proteico Relacionado con la Actina/metabolismo , Actinas/metabolismo , Retículo Endoplásmico/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Modelos Moleculares , Fagosomas/metabolismo , Autofagia/fisiología , Compuestos Bicíclicos Heterocíclicos con Puentes , Calnexina/metabolismo , Línea Celular , Ensayo Cometa , Depsipéptidos , Dispersión Dinámica de Luz , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Proteínas Luminiscentes , Proteínas de la Membrana/química , Proteínas Asociadas a Microtúbulos/química , Nocodazol , Estructura Terciaria de Proteína , Canales de Translocación SEC , Tiazolidinas , Proteína Fluorescente RojaRESUMEN
The relationship between RNA expression and cell function can often be difficult to decipher due to the presence of both temporal and sub-cellular processing of RNA. These intricacies of RNA regulation can often be overlooked when only acquiring global measurements of RNA expression. This has led to development of several tools that allow for the real-time imaging of individual engineered RNA transcripts in living cells. Here, we describe a new technique that utilizes an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), to image RNA transcripts that were engineered to contain 96-tandem repeats of the RBMB target sequence in the 3'-untranslated region. Binding of RBMBs to the target RNA resulted in discrete bright fluorescent spots, representing individual transcripts, that could be imaged in real-time. Since RBMBs are a synthetic probe, the use of photostable, bright, and red-shifted fluorophores led to a high signal-to-background. RNA motion was readily characterized by both mean squared displacement and moment scaling spectrum analyses. These analyses revealed clear examples of directed, Brownian, and subdiffusive movements.
Asunto(s)
ARN Mensajero/metabolismo , Técnicas Biosensibles , Línea Celular Tumoral , Clonación Molecular , Humanos , Microscopía Fluorescente , Sondas de Oligonucleótidos , Transporte de ARN , ARN Mensajero/genética , Secuencias Repetidas en Tándem , Imagen de Lapso de TiempoRESUMEN
BACKGROUND: In the intracellular environment, motor-driven cargo must navigate a dense cytoskeletal network among abundant organelles. RESULTS: We investigated the effects of the crowded intracellular environment on early endosomal trafficking. Live-cell imaging of an endosomal cargo (endocytosed epidermal growth factor-conjugated quantum dots) combined with high-resolution tracking was used to analyze the heterogeneous motion of individual endosomes. The motile population of endosomes moved toward the perinuclear region in directed bursts of microtubule-based, dynein-dependent transport interrupted by longer periods of diffusive motion. Actin network density did not affect motile endosomes during directed runs or diffusive interruptions. Simultaneous two-color imaging was used to correlate changes in endosomal movement with potential obstacles to directed runs. Termination of directed runs spatially correlated with microtubule-dense regions, encounters with other endosomes, and interactions with the endoplasmic reticulum. During a subset of run terminations, we also observed merging and splitting of endosomes, deformation of the endoplasmic reticulum, and directional reversals at speeds up to 10-fold greater than characteristic in vitro motor velocities. These observations suggest that endosomal membrane tension is high during directed run termination. CONCLUSIONS: Our results indicate that the crowded cellular environment significantly impacts the motor-driven motility of organelles. Rather than simply acting as impediments to movement, interactions of trafficking cargos with intracellular obstacles may facilitate communication between membrane-bound compartments or contribute to the generation of membrane tension necessary for fusion and fission of endosomal membranes or remodeling of the endoplasmic reticulum.
Asunto(s)
Citoplasma/metabolismo , Endosomas/metabolismo , Vesículas Transportadoras/metabolismo , Animales , Transporte Biológico , Células COS , Línea Celular , Chlorocebus aethiops , Dineínas/metabolismo , Retículo Endoplásmico/metabolismo , Factor de Crecimiento Epidérmico/metabolismo , Puntos Cuánticos/metabolismoRESUMEN
Physical features of microenvironments such as matrix elasticity E can clearly influence cell morphology and cell phenotype, but many differences between model matrices raise questions as to whether a standard biological scale for E exists, especially in 3D as well as in 2D. An E-series of two distinct types of hydrogels are ligand-functionalized here with non-fibrous collagen and used to elucidate wide-ranging cell and cytoskeletal responses to E in both 2D and 3D matrix geometries. Cross-linked hyaluronic acid (HA) based matrices as well as standard polyacrylamide (PA) hydrogels show that, within hours of initial plating, the adhesion, asymmetric shape, and cytoskeletal order within mesenchymal stem cells generally depend on E nonmonotonically over a broad range of physiologically relevant E. In particular, with overlays of a second matrix the stiffer of the upper or lower matrix dominates key cell responses to 3D: the cell invariably takes an elongated shape that couples to E in driving cytoplasmic stress fiber assembly. In contrast, embedding cells in homogeneous HA matrices constrains cells to spherically symmetric shapes in which E drives the assembly of a predominantly cortical cytoskeleton. Non-muscle myosin II generates the forces required for key cell responses and is a target of a phospho-Tyrosine signaling pathway that likely regulates contractile assemblies and also depends nonmonotonically on E. The results can be understood in part from a theory for stress fiber polarization that couples to matrix elasticity as well as cell shape and accurately predicts cytoskeletal order in 2D and 3D, regardless of polymer system.
Asunto(s)
Elasticidad/fisiología , Matriz Extracelular/fisiología , Ácido Hialurónico/química , Células Madre Mesenquimatosas/citología , Miosina Tipo IIA no Muscular/metabolismo , Fosforilación/efectos de los fármacos , Fibras de Estrés/fisiología , Resinas Acrílicas/química , Resinas Acrílicas/farmacología , Actinas/metabolismo , Adhesión Celular/efectos de los fármacos , Adhesión Celular/fisiología , Proliferación Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Forma de la Célula/fisiología , Supervivencia Celular/efectos de los fármacos , Colágeno Tipo I/química , Colágeno Tipo I/metabolismo , Colágeno Tipo I/farmacología , Módulo de Elasticidad/fisiología , Matriz Extracelular/química , Gelatina/química , Gelatina/farmacología , Compuestos Heterocíclicos de 4 o más Anillos/farmacología , Humanos , Ácido Hialurónico/farmacología , Hidrogeles/síntesis química , Hidrogeles/química , Hidrogeles/farmacología , Células Madre Mesenquimatosas/efectos de los fármacos , Células Madre Mesenquimatosas/metabolismo , Microscopía de Fuerza Atómica , Microscopía Fluorescente , Cadenas Pesadas de Miosina/metabolismo , Miosina Tipo IIA no Muscular/antagonistas & inhibidores , Fosfotirosina/metabolismo , Fibras de Estrés/efectos de los fármacos , Vinculina/metabolismoRESUMEN
Huntingtin (Htt) is a membrane-associated scaffolding protein that interacts with microtubule motors as well as actin-associated adaptor molecules. We examined a role for Htt in the dynein-mediated intracellular trafficking of endosomes and lysosomes. In HeLa cells depleted of either Htt or dynein, early, recycling, and late endosomes (LE)/lysosomes all become dispersed. Despite altered organelle localization, kinetic assays indicate only minor defects in intracellular trafficking. Expression of full-length Htt is required to restore organelle localization in Htt-depleted cells, supporting a role for Htt as a scaffold that promotes functional interactions along its length. In dynein-depleted cells, LE/lysosomes accumulate in tight patches near the cortex, apparently enmeshed by cortactin-positive actin filaments; Latrunculin B-treatment disperses these patches. Peripheral LE/lysosomes in dynein-depleted cells no longer colocalize with microtubules. Htt may be required for this off-loading, as the loss of microtubule association is not seen in Htt-depleted cells or in cells depleted of both dynein and Htt. Inhibition of kinesin-1 relocalizes peripheral LE/lysosomes induced by Htt depletion but not by dynein depletion, consistent with their detachment from microtubules upon dynein knockdown. Together, these data support a model of Htt as a facilitator of dynein-mediated trafficking that may regulate the cytoskeletal association of dynamic organelles.
Asunto(s)
Citoesqueleto/metabolismo , Dineínas/genética , Endosomas/metabolismo , Lisosomas/metabolismo , Proteínas Motoras Moleculares/metabolismo , Proteínas del Tejido Nervioso/genética , Proteínas Nucleares/genética , Actinas/metabolismo , Línea Celular Tumoral , Dineínas/metabolismo , Técnicas de Silenciamiento del Gen/métodos , Células HeLa , Humanos , Proteína Huntingtina , Proteínas de Membrana de los Lisosomas/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Proteínas Asociadas a Microtúbulos/fisiología , Microtúbulos/metabolismo , Microtúbulos/fisiología , Proteínas Motoras Moleculares/genética , Proteínas del Tejido Nervioso/metabolismo , Proteínas Nucleares/metabolismo , Orgánulos/metabolismo , Polimerizacion , Transporte de Proteínas/fisiología , Interferencia de ARNRESUMEN
Leiomodin (Lmod) is a muscle-specific F-actin-nucleating protein that is related to the F-actin pointed-end-capping protein tropomodulin (Tmod). However, Lmod contains a unique â¼150-residue C-terminal extension that is required for its strong nucleating activity. Overexpression or depletion of Lmod compromises sarcomere organization, but the mechanism by which Lmod contributes to myofibril assembly is not well understood. We show that Tmod and Lmod localize through fundamentally different mechanisms to the pointed ends of two distinct subsets of actin filaments in myofibrils. Tmod localizes to two narrow bands immediately adjacent to M-lines, whereas Lmod displays dynamic localization to two broader bands, which are generally more separated from M-lines. Lmod's localization and F-actin nucleation activity are enhanced by interaction with tropomyosin. Unlike Tmod, the myofibril localization of Lmod depends on sustained muscle contraction and actin polymerization. We further show that Lmod expression correlates with the maturation of myofibrils in cultured cardiomyocytes and that it associates with sarcomeres only in differentiated myofibrils. Collectively, the data suggest that Lmod contributes to the final organization and maintenance of sarcomere architecture by promoting tropomyosin-dependent actin filament nucleation.
Asunto(s)
Proteínas de Microfilamentos/metabolismo , Proteínas Musculares/metabolismo , Miocitos Cardíacos/citología , Miocitos Cardíacos/metabolismo , Sarcómeros/metabolismo , Tropomodulina/metabolismo , Actinas/metabolismo , Animales , Sitios de Unión , Diferenciación Celular , Núcleo Celular/metabolismo , Contracción Muscular , Proteínas Mutantes/metabolismo , Miofibrillas/metabolismo , Miosina Tipo II/metabolismo , Unión Proteica , Transporte de Proteínas , Ratas , Fracciones Subcelulares/metabolismo , Tropomiosina/metabolismo , Pez CebraRESUMEN
Shape effects of synthetic carriers are largely unexplored in vivo, although recent findings suggest that flexible filaments can persist in the circulation even if microns in length. Here, to better assess biodistribution, a near-infrared fluorophore (NIRF) was incorporated into such block copolymer "filomicelles", and both in vivo and ex vivo imaging show that the majority of these wormlike micelles remain in the circulation for at least a day after intravenous injection. NIRF imaging further suggests that filomicelles convect into a tumor and some fragments can penetrate into the tumor stroma. To assess a functional effect, the hydrophobic drug paclitaxel (tax) was loaded into both filomicelles and sonication-generated spherical micelles of the same copolymer. Intravenous injection of tax-loaded filomicelles nearly doubles the maximum tolerated dose of tax in normal mice compared to tax-loaded spherical micelles. In tumor-bearing mice, the higher dose of tax produces greater and more sustained tumor shrinkage and tumor cell apoptosis. These results thus begin to address mechanisms for how nonspherical carriers deliver both imaging agents and anticancer therapeutics to solid tumors.
Asunto(s)
Portadores de Fármacos/química , Micelas , Nanoestructuras/química , Animales , Antineoplásicos Fitogénicos/administración & dosificación , Antineoplásicos Fitogénicos/farmacocinética , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Femenino , Colorantes Fluorescentes/administración & dosificación , Colorantes Fluorescentes/farmacocinética , Humanos , Ratones , Ratones Desnudos , Microscopía Fluorescente , Trasplante de Neoplasias , Neoplasias Experimentales/tratamiento farmacológico , Neoplasias Experimentales/metabolismo , Neoplasias Experimentales/patología , Paclitaxel/administración & dosificación , Paclitaxel/farmacocinética , Distribución Tisular , Trasplante HeterólogoRESUMEN
Cells may lack eyes to see and ears to hear, but cells do seem to have a sense of 'touch' that allows them to feel their microenvironment. This is achieved in part through contractility coupled adhesion to physically flexible 'soft' tissue. Here we summarize some of the known variations in elasticity of solid tissue and review some of the long-term effects of cells 'feeling' this elasticity, focusing on differentiation processes of both committed cell types and stem cells. We then highlight what is known of molecular remodeling in cells under stress on short time scales. Key roles for forces generated by ubiquitous and essential myosin-II motors in feedback remodeling are emphasized throughout.
Asunto(s)
Diferenciación Celular/fisiología , Miosinas/metabolismo , Animales , Fenómenos Biomecánicos , Adhesión Celular , Fenómenos Fisiológicos Celulares , Elasticidad , Humanos , Modelos Biológicos , Células Madre/citologíaRESUMEN
Human CMV establishes a lifelong latent infection in the majority of people worldwide. Although most infections are asymptomatic, immunocompetent hosts devote an extraordinary amount of immune resources to virus control. To increase our understanding of CMV immunobiology in an animal model, we used a genomic approach to comprehensively map the C57BL/6 CD8 T cell response to murine CMV (MCMV). Responses to 27 viral proteins were detectable directly ex vivo, the most diverse CD8 T cell response yet described within an individual animal. Twenty-four peptide epitopes were mapped from 18 Ags, which together account for most of the MCMV-specific response. Most Ags were from genes expressed at early times, after viral genes that interfere with Ag presentation are expressed, consistent with the hypothesis that the CD8 T cell response to MCMV is largely driven by cross-presented Ag. Titration of peptide epitopes in a direct ex vivo intracellular cytokine staining assay revealed a wide range of functional avidities, with no obvious correlation between functional avidity and the strength of the response. The immunodominance hierarchy varied only slightly between mice and between experiments. However, H-2(b)-expressing mice with different genetic backgrounds responded preferentially to different epitopes, indicating that non-MHC-encoded factors contribute to immunodominance in the CD8 T cell response to MCMV.