Isolation and characterization of exopolysaccharide with immunomodulatory activity from fermentation broth of Morchella conica.

UNLABELLED: BACKGROUND AND THE PURPOSE OF THIS STUDY: Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. In vitro and in vivo studies suggest that certain polysaccharides affect immune system function. Morchella conica (M. conica) is a species of rare edible mushroom whose multiple medicinal functions have been proven. Thus, the objective of this study is to isolate and characterize of exopolysaccharide from submerged mycelial culture of M. conica, and to evaluate its immunomodulatory activity. METHODS: A water-soluble Morchella conica Polysaccharides (MCP) were extracted and isolated from the fermentation broth of M. conica through a combination of DEAE-cellulose and Sephacryl S-300 HR chromatograph. NMR and IR spectroscopy has played a developing role in identification of polysaccharide with different structure and composition from fungal and plant sources, as well as complex glycosaminoglycans of animal origin. Thus, NMR and IR spectroscopy were used to analyze the chemical structure and composition of the isolated polysaccharide. Moreover, the polysaccharide was tested for its immunomodulatory activity at different concentrations using in vitro model. RESULTS: The results showed that MCP may significantly modulate nitric oxide production in macrophages, and promote splenocytes proliferation. Analysis from HPLC, infrared spectra and nuclear magnetic resonance spectroscopy showed that MCP was a homogeneous mannan with an average molecular weight of approximately 81.2 kDa. The glycosidic bond links is →6)-α-D-Man p-(1→. CONCLUSION: The results suggested that the extracted MCP may modulate nitric oxide production in macrophages and promote splenocytes proliferation, and it may act as a potent immunomodulatory agent.

[Investigation on multiband attenuation compatibility of carbon nanotubes].

The present investigation was focused on how to realize the attenuating compatibility of the electromagnetic defilade material in UV-Vis-Infrared band. Optical spectra of CNTs was investigated on the basis of analytical technologies such as Fourier transform infrared spectroscopy, UV-Vis spectrophotometer, laser scattering, and electron microscopy. It was demonstrated that the multi-band attenuation characteristic of CNTs is effectively associated with additives, concentrations and configurations. The measured sample with a concentration of 0.04 g x L(-1), in which the radius of CNTs was 30-50 nm, bore the maximum value of the extinction coefficient of 7.825 m2 x g(-1) at the point of 265 nm. Meanwhile, this kind of CNTs presents outstanding unified attenuation properties in infrared wave band when the thickness of the CNTs film is 0.1 mm and the total mass of CNTs is 0.349 mg in optical path. Especially, the unified attenuation goes beyond 90% in 4.0-6.25 and 7.0-16.7 microm.

[The effect of Doppler effect on ultraviolet absorption spectrum from difference in temperature (UVASDT)].

In this paper, the formation of UV absorption spectrum from difference in temperature (UVASDT) is discussed. Broadening of spectral lines might be one of the reasons for the formation of UVASDT. The effect of temperature on the broadening of spectral lines is analyzed. The Doppler SDT function is deduced, and the SDT of C60 and progesterone can be explained by it. It is indicated that the Doppler effect might be the primary reason for the formation of UVASDT of this kind of substance.