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Characterizing the microstructure of radiation- and chemical-sensitive lithium dendrites and its solid electrolyte interphase (SEI) is an important task when investigating the performance and reliability of lithium-ion batteries. Widely used methods, such as cryogenic high-resolution transmission electron microscopy as well as related spectroscopy, are able to reveal the local structure at nanometer and atomic scale; however, these methods are unable to show the distribution of various crystal phases along the dendrite in a large field of view. In this work, two types of four-dimensional electron microscopy diffractive imaging methods, i.e., scanning electron nanodiffraction (SEND) and scanning convergent beam electron diffraction (SCBED), are employed to show a new pathway on characterizing the sensitive lithium dendrite samples at room temperature and in a large field of view. Combining with the non-negative matrix factorization (NMF) algorithm, orientations of different lithium metal grains along the lithium dendrite as well as different lithium compounds in the SEI layer are clearly identified.
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Understanding the structure-property relationship of lithium-ion conducting solid oxide electrolytes is essential to accelerate their development and commercialization. However, the structural complexity of nonideal materials increases the difficulty of study. Here, we develop an algorithmic framework to understand the effect of microstructure on the properties by linking the microscopic morphology images to their ionic conductivities. We adopt garnet and perovskite polycrystalline oxides as examples and quantify the microscopic morphologies via extracting determined physical parameters from the images. It directly visualizes the effect of physical parameters on their corresponding ionic conductivities. As a result, we can determine the microstructural features of a Li-ion conductor with high ionic conductivity, which can guide the synthesis of highly conductive solid electrolytes. Our work provides a novel approach to understanding the microstructure-property relationship for solid-state ionic materials, showing the potential to extend to other structural/functional ceramics with various physical properties in other fields.
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Two-dimensional (2D) membranes have shown promising potential for ion-selective separation but often suffer from the trade-off between permeability and selectivity. Herein, we report an ultrathin 2D sulfonate-functionalized metal-organic framework (MOF) membrane for efficient lithium-ion sieving. The narrow pores with angstrom precision in the MOF assist hydrated ions to partially remove the hydration shell, according to different hydration energies. The abundant sulfonate groups in the MOF channels serve as hopping sites for fast lithium-ion transport, contributing to a high Li-ion permeability. Then, the difference in affinity of the Li+, Na+, K+, and Mg2+ ions to the terminal sulfonate groups further enhances the Li-ion selectivity. The reported ultrathin MOF membrane overcomes the trade-off between permeability and selectivity and opens up a new avenue for highly permselective membranes.
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Investigating the growth kinetics of Li metal in solid-state batteries is crucial to both a fundamental understanding and practical application. Here, by directly observing the formation of Li metal from Ta-doped Li6.4La3Zr1.4Ta0.6O12 (LLZTO) in a transmission electron microscope, the growth kinetics is analyzed quantitatively. The growth kinetics of Li deposits shows a cubic-curve characteristic for LLZTO with Li-source-free. Instead, a linear growth process is observed with Li-source supplied. The impact of the illuminating electron dose rate on the growth kinetics is clarified, indicating that even low dose rates (1-3 e-/Å2/s) could affect Li growth, highlighting the significance of controlling dose rates. Furthermore, a new pathway for the formation of Li metal from Li-containing materials utilizing the field-emission effect is reported. This work has implications on the failure mechanism in solid batteries by using limited Li anodes and opens pathways for regulating Li growth in LLZTO at various scenarios, which can also extend to other ionic conductors.
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Current therapy for hepatic injury induced by the accumulation of bile acids is limited. Leucine-rich repeat G protein-coupled receptor 4 (LGR4), also known as GPR48, is critical for cytoprotection and cell proliferation. Here, we reported a novel function for the LGR4 in cholestatic liver injury. In the bile duct ligation (BDL)-induced liver injury model, hepatic LGR4 expression was significantly downregulated. Deficiency of LGR4 in hepatocytes (Lgr4LKO) notably decreased BDL-induced liver injury measured by hepatic necrosis, fibrosis, and circulating liver enzymes and total bilirubin. Levels of total bile acids in plasma and liver were markedly reduced in these mice. However, deficiency of LGR4 in macrophages (Lyz2-Lgr4MKO) demonstrated no significant effect on liver injury induced by BDL. Deficiency of LGR4 in hepatocytes significantly attenuated S1PR2 and the phosphorylation of protein kinase B (AKT) induced by BDL. Recombinant Rspo1 and Rspo3 potentiated the taurocholic acid (TCA)-induced upregulation in S1PR2 and phosphorylation of AKT in hepatocytes. Inhibition of S1PR2-AKT signaling by specific AKT or S1PR2 inhibitors blocked the increase of bile acid secretion induced by Rspo1/3 in hepatocytes. Our studies indicate that the R-spondins (Rspos)-LGR4 signaling in hepatocytes aggravates the cholestatic liver injury by potentiating the production of bile acids in a S1PR2-AKT-dependent manner.NEW & NOTEWORTHY Deficiency of LGR4 in hepatocytes alleviates BDL-induced liver injury. LGR4 in macrophages demonstrates no effect on BDL-induced liver injury. Rspos-LGR4 increases bile acid synthesis and transport via potentiating S1PR2-AKT signaling in hepatocytes.
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Enfermedad Hepática Crónica Inducida por Sustancias y Drogas , Colestasis , Ratones , Animales , Proteínas Proto-Oncogénicas c-akt/metabolismo , Hígado/metabolismo , Colestasis/complicaciones , Colestasis/metabolismo , Hepatocitos/metabolismo , Ácidos y Sales Biliares/metabolismo , Conductos Biliares/metabolismo , Ligadura , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismoRESUMEN
The uncontrolled deposition/dissolution process of lithium dendrites during electrochemical cycling in batteries limits the large-scale application of Li metal anodes. Investigating the microstructure of Li dendrites is a focal point. Currently, the only way to protect and observe sensitive Li dendrites is through low-temperature transmission electron microscopy (LT-TEM), whereas room-temperature characterization is still lacking. In this work, the room-temperature microstructure of Li dendrites was obtained by TEM using both vacuum- and inert-gas-transfer methods. Detailed comparison between LT- and room-temperature (RT-)TEM characterizations was provided to show the pros and cons of each method. Especially, RT-TEM shows the advantage of flexible incorporation with multifunctional characterizations, such as 3D tomography. By using RT-TEM, microstructural evolution of Li dendrites during the electrodeposition/dissolution process, including increase of the quantity of inorganic Li2O compounds in the solid electrolyte interphase, lateral growth behavior, and two types of inactive Li, has been revealed, enriching the understanding of the structure-property relationship of Li dendrites.
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Mechanism underlying the metabolic benefit of intermittent fasting remains largely unknown. Here, we reported that intermittent fasting promoted IL-22 production by ILC3s and subsequent beigeing of subcutaneous white adipose tissue. Adoptive transfer of intestinal ILC3s increased beigeing of white adipose tissue in diet-induced-obese mice. Exogenous IL-22 significantly increased the beigeing of subcutaneous white adipose tissue. Deficiency of IL-22 receptor attenuated the beigeing induced by intermittent fasting. Single-cell sequencing of sorted intestinal immune cells revealed that intermittent fasting increased aryl hydrocarbon receptor signaling in ILC3s. Analysis of cellâcell ligand receptor interactions indicated that intermittent fasting may stimulate the interaction of ILC3s with dendritic cells (DCs) and macrophages. These results establish the role of intestinal ILC3s in beigeing of white adipose tissue, suggesting that ILC3/IL-22/IL-22R axis contributes to the metabolic benefit of intermittent fasting.
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Mechanism underlying the metabolic benefit of intermittent fasting remains largely unknown. Here, we reported that intermittent fasting promoted interleukin-22 (IL-22) production by type 3 innate lymphoid cells (ILC3s) and subsequent beigeing of subcutaneous white adipose tissue. Adoptive transfer of intestinal ILC3s increased beigeing of white adipose tissue in diet-induced-obese mice. Exogenous IL-22 significantly increased the beigeing of subcutaneous white adipose tissue. Deficiency of IL-22 receptor (IL-22R) attenuated the beigeing induced by intermittent fasting. Single-cell sequencing of sorted intestinal immune cells revealed that intermittent fasting increased aryl hydrocarbon receptor signaling in ILC3s. Analysis of cell-cell ligand receptor interactions indicated that intermittent fasting may stimulate the interaction of ILC3s with dendritic cells and macrophages. These results establish the role of intestinal ILC3s in beigeing of white adipose tissue, suggesting that ILC3/IL-22/IL-22R axis contributes to the metabolic benefit of intermittent fasting.
Obesity refers to a condition where a person has excessive fat accumulation, which can have negative impacts on their health. Managing obesity has typically relied on reducing energy intake and increasing energy use through diets and exercise. For example, intermittent fasting is a diet strategy involving periods of time in a day or week where a person does not eat any food. Research has shown that intermittent fasting may improve the metabolism and increase energy use by enhancing a process known as "beigeing" of white fat tissue. In this process, white fat cells or their precursor cells differentiate into beige fat cells, which can consume excess energy by burning fat. Consequently, understanding how beigeing of white fat cells is activated in intermittent fasting may reveal a promising strategy for tackling obesity and metabolic diseases. Immune cells found in the gut known as innate lymphoid cells (ILCs) may play a role in the metabolic benefits from intermittent fasting. However, the roles of ILCs are complex: some types of ILCs can promote obesity, while others show metabolic benefits through their release of proteins like IL-17 and IL-22, which can help the body to metabolise glucose. To find out if these immune cells play a role in intermittent fasting, Chen, Sun et al. used diet-induced obese mice that had to fast every other day. Intermittent fasting was found to cause a form of ILCs (ILC3s) to release IL-22, which resulted in beigeing of white fat cells in obese mice. Single-cell sequencing techniques of gut immune cells further revealed that intermittent fasting increased forms of signalling in ILC3s and caused ILC3s to interact with other immune cells, such as dendritic cells and macrophages. The findings demonstrate how intermittent fasting causes beigeing of white adipose tissue through ILC3s, revealing mechanisms underpinning the metabolic benefits found from intermittent fasting. More research into this process may help identify new targets for treating obesity.
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Interleucina-22 , Linfocitos , Ratones , Animales , Linfocitos/metabolismo , Inmunidad Innata , Ayuno Intermitente , Tejido Adiposo Blanco/metabolismoRESUMEN
To explore the shale gas occurrence mechanism in shale with an intact pore structure under actual reservoir conditions, an adsorption experiment on massive shale was performed. Considering the change in the pore volume of massive shale under effective stress, the adsorption mechanism and free gas storage space of massive shale were investigated. Based on the adsorption mechanism assumptions of micropore filling and mesopore multilayer adsorption, the adsorbed phase densities of pores of varying pore sizes were calculated and applied to the conversion of the absolute adsorption amount of massive shale. The results show the existence of isolated pores in the massive shale, resulting in a lower adsorption capacity in comparison to granular samples. When subjected to the combined effects of in situ stress and pore pressure, the pore volume of massive shale gradually decreases with the increase in effective stress. Shale gas is mainly adsorbed in micropores, but with increasing pressure, the adsorption amount of micropores approaches saturation, and the contribution of mesopores to the total adsorption amount gradually increases. The main adsorption mechanism of shale gas is based on micropore filling, and the multilayer surface adsorption of mesopores should also be considered. By combining the simplified local density model and the Ono-Kondo lattice model, the adsorption behavior of shale gas can be accurately described. To accurately estimate shale gas reserves, it is necessary to take into account the actual pore size distribution, pore volume compressibility, and connected porosity of the shale samples.
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Permeability is a critical parameter of tight reservoir rocks and one of the important parameters for characterizing fluid flow and production from reservoirs. It determines the feasibility of its commercial development. SC-CO2 has been used in shale gas exploitation for efficient fracturing and the added benefit of CO2 geo-storage. And SC-CO2 plays an important role in permeability evolution of shale gas reservoirs. In this paper, Firstly, the permeability characteristics of shale under CO2 injection are discussed. The experimental results show that the relationship between permeability and gas pressure is not a single exponential relationship, but there is an obvious segmentation phenomenon, which is particularly obvious when it is close to the supercritical state, and the overall trend is first decreased and then increased. Subsequently, other specimens were selected for SC-CO2 immersion, and nitrogen was used to calibrate and compare shale permeability before and after treatment to assess changes in shale permeability after SC-CO2 treatment at pressures from 7.5 to 11.5 MPa and X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM) were used to analyze the raw and CO2-treated shale particle sample, respectively. Results indicate the permeability increases significantly after SC-CO2 treated, and permeability growth is a linear function of SC-CO2 pressure. According to (XRD) analysis and (SEM) analysis, SC-CO2 not only can act as a solvent and dissolve carbonate minerals and clay minerals, but also can complex chemical reactions with mineral components in shale, Further dissolution of carbonate minerals and clay minerals, widened gas seepage channels and enhancing the permeability.
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Single-nucleotide polymorphisms in G protein-coupled receptor 180 (GPR180) are associated with hypertriglyceridemia. The aim of this study was to determine whether hepatic GPR180 impacts lipid metabolism. Hepatic GPR180 was knocked down using two approaches: Gpr180-specific short hairpin (sh)RNA carried by adeno-associated virus 9 (AAV9) and alb-Gpr180-/- transgene established by crossbreeding albumin-Cre mice with Gpr180flox/flox animals, in which Gpr180 was specifically knocked down in hepatocytes. Adiposity, hepatic lipid contents, and proteins related to lipid metabolism were analyzed. The effects of GPR180 on triglyceride and cholesterol synthesis were further verified by knocking down or overexpressing Gpr180 in Hepa1-6 cells. Gpr180 mRNA was upregulated in the liver of HFD-induced obese mice. Deficiency of Gpr180 decreased triglyceride and cholesterol contents in the liver and plasma, ameliorated hepatic lipid deposition in HFD-induced obese mice, increased energy metabolism, and reduced adiposity. These alterations were associated with downregulation of transcription factors SREBP1 and SREBP2, and their target acetyl-CoA carboxylase. In Hepa1-6 cells, Gpr180 knockdown decreased intracellular triglyceride and cholesterol contents, whereas its overexpression increased their levels. Overexpression of Gpr180 significantly reduced the PKA-mediated phosphorylation of substrates and consequent CREB activity. Hence, GPR180 might represent a novel drug target for intervention of adiposity and liver steatosis.
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Dieta Alta en Grasa , Enfermedad del Hígado Graso no Alcohólico , Ratones , Animales , Dieta Alta en Grasa/efectos adversos , Enfermedad del Hígado Graso no Alcohólico/metabolismo , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Ratones Obesos , Ratones Endogámicos , Hígado/metabolismo , Metabolismo de los Lípidos/genética , Obesidad/metabolismo , Triglicéridos/metabolismo , Colesterol/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Ratones Endogámicos C57BLRESUMEN
Interlayer electric fields in two-dimensional (2D) materials create photoelectron protecting barriers useful to mitigate electron-hole recombination. However, tuning the interlayer electric field remains challenging. Here, carbon-doped Bi3O4Cl (C:Bi3O4Cl) nanosheets are synthesized using a gas phase protocol, and n-type carriers are acquired as confirmed by the transconductance polarity of nanosheet field effect transistors. Thin C:Bi3O4Cl nanosheets show excellent 266 nm photodetector figures of merit, and an avalanche-like photocurrent is demonstrated. Decaying behaviors of photoelectrons pumped by a 266 nm laser pulse (266 nm photoelectrons) are observed using transient absorption spectroscopy, and a significant 266 nm photoelectron lifetime quality in C:Bi3O4Cl is presented. Built C:Bi3O4Cl models suggest that the interlayer electric field can be boosted by two different carbon substitutions at the inner and outer bismuth sites. This work reports a facile approach to increase the interlayer electric field in Bi3O4Cl for future UV-C photodetector applications.
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Confined liquid has attracted great attention due to its potential applications in nanofluidic devices. With the development of liquid-cell transmission electron microscopy (LC-TEM), investigating the behaviors of confined liquid can be realized in real time. However, the dynamics of the liquid layer in liquid cells have not been fully understood. Here, nanoparticles (NPs) adhered to the cell window membranes are used as reference objects to study the flow regime of the liquid layer, which causes cooperative motion of the membranes and the NPs. Two categories of motion behaviors are investigated. One is the contraction of NPs toward the interior viewing area which results from the spreading out of the liquid to the surrounding region, with the bending of the membranes increasing with the loss of liquid in the viewing area. The other motion behavior is the occasional movement of all the NPs in the same direction with the directional movement of the liquid layer. This work offers a new method to study the dynamics of liquids by LC-TEM, the discoveries of which are valuable for understanding the confined liquid dynamics.
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There is a keen interest in the use of electrochromic materials because they can regulate light and heat, thereby reducing the cooling and heating energy. However, the long response time, short cycle life, and high power consumption of an electrochromic film hinder its development. Here, we report an electrochromic material of complex niobium tungsten oxides. The Nb18W16O93 thin films in the voltage range of 0 to -1.5 V show good redox kinetics with the coloration time of 4.7 s and bleaching time of 4.0 s, respectively. The electrochromic device based on the Nb18W16O93 thin film has an optical modulation of 53.1% at a wavelength of 633 nm, with the coloration efficiency of â¼46.57 cm2 C-1. An excellent electrochemical stability of 78.1% retention after 8000 cycles is also achieved. These good performances are due to the fast and stable Li-ion intercalation/extraction in the open framework of Nb18W16O93 with multiple ion positions. Our work provides a strategy for electrochromic materials with fast response time and good cycle stability.
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Here, we report a two-dimension (2D) superlattice-like membrane composed of periodic MoS2 and GO nanosheets, which delivers enhanced salt rejection capability, high water flux, and Li ion selectivity. It opens a new perspective in assembling 2D membranes and can be utilized as a green and low-cost approach for desalination.
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Asprosin is a new hormone released from white adipose tissue (WAT) that not only promotes glucose release in the liver but also activates orexigenic neurons in the hypothalamus to promote appetite and weight gain. Its effect on skeletal muscle glucose uptake is unclear. This research, a stable asprosin expression system was formed by first constructing a eukaryotic expression vector pPIC9K-8His-Asprosin, and then transforming it into the Pichia pastoris strain GS115. Pichia pastoris methanol induction combined with Nickel-NTA magnetic beads purification strategy was used to express and purify asprosin protein. Purified asprosin can promote the phosphorylation of PKA substrate, and intraperitoneal injection of asprosin can increase blood glucose. After proteolysis and detection by mass spectrometry, asprosin was found to have 3 glycosylation sites and multiple glycosyl types. Asprosin up-regulated glucose transporter 4 (GLUT4) expression in myotubes, including mRNA and protein levels. In addition, asprosin enhanced AMP-activated protein kinase (AMPK) phosphorylation, but it had no effect on AKT phosphorylation with or without insulin treatment. Treatment with an AMPK inhibitor (compound C) reduced the asprosin-mediated glucose uptake effect. These results show that purified asprosin activated AMPK signaling in skeletal muscle and further promoted glucose uptake. From the perspective of skeletal muscle uptake of glucose, asprosin may have beneficial effects on type 2 diabetes.