Determination of dimethylated nucleosides in serum from colorectal cancer patients by hydrophilic interaction liquid chromatography-tandem mass spectrometry.

RNA modifications play a crucial regulatory role in a variety of biological processes and are closely related to numerous diseases, including cancer. The diversity of metabolites in serum makes it a favored biofluid for biomarkers discovery. In this work, a robust and accurate hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) approach was established for simultaneous determination of dimethylated nucleosides in human serum. Using the established method, we were able to accurately quantify the concentrations of N6-2'-O-dimethyladenosine (m6Am), N2,N2-dimethylguanosine (m2,2G), and 5,2'-O-dimethyluridine (m5Um) in serum samples from 53 healthy controls, 57 advanced colorectal adenoma patients, and 39 colorectal cancer (CRC) patients. The results showed that, compared with healthy controls and advanced colorectal adenoma patients, the concentrations of m6Am and m2,2G were increased in CRC patients, while the concentration of m5Um was decreased in CRC patients. These results indicate that these three dimethylated nucleosides could be potential biomarkers for early detection of colorectal cancer. Interestingly, the level of m5Um was gradually decreased from healthy controls to advanced colorectal adenoma patients to CRC patients, indicating m5Um could also be used to evaluate the level of malignancy of colorectal tumor. In addition, this study will contribute to the investigation on the regulatory mechanisms of RNA dimethylation in the onset and development of colorectal cancer.

Clinical effect of sidong wubu method in treatment of closed fracture of upper limbs.

OBJECTIVE: To objectively evaluate the clinically curative effect of sidong wubu method on closed fracture of upper limbs. METHODS: A single-blind randomized controlled trial was used to divide 654 patients with closed fracture of upper limbs at early stage into two groups. 298 patients in the surgical group were treated with open reduction and internal fixation and 356 patients in the treatment group with sidong wubu method for 6 months as a course of treatment. RESULTS: As for short-term curative effect (after 6 courses of treatment), the total effective rate was 97.7% in the treatment group and 92.9% in the control group, and the excellent and good rate was 83.7% and 76.5% respectively. Fracture-healing time, treatment cost, function-recovering time, scores of symptoms and signs obviously declined in both groups with remarkable difference between the two groups. As to long-term curative effect (after follow-up visit for one year to 5 years and 2 months), there was still noticeable difference (chi2= 7.536, P<0.05) in total curative effect and in excellent and good rate between the two groups. CONCLUSION: With low cost, short treatment course, good function and other advantages, sidong wubu method can be first used to treat closed fracture of upper limbs.