RESUMEN
Gametophytic self-incompatibility (GSI) has been widely studied in flowering plants, but studies of the mechanisms underlying pollen tube growth arrest by self S-RNase in GSI species are limited. In the present study, two leucine-rich repeat extensin genes in pear (Pyrus bretschneideri), PbLRXA2.1 and PbLRXA2.2, were identified based on transcriptome and quantitative real-time PCR analyses. The expression levels of these two LRX genes were significantly higher in the pollen grains and pollen tubes of the self-compatible cultivar 'Jinzhui' (harboring a spontaneous bud mutation) than in those of the self-incompatible cultivar 'Yali'. Both PbLRXA2.1 and PbLRXA2.2 stimulated pollen tube growth and attenuated the inhibitory effects of self S-RNase on pollen tube growth by stabilizing the actin cytoskeleton and enhancing cell wall integrity. These results indicate that abnormal expression of PbLRXA2.1 and PbLRXA2.2 is involved in the loss of self-incompatibility in 'Jinzhui'. The PbLRXA2.1 and PbLRXA2.2 promoters were directly bound by the ABRE-binding factor PbABF.D.2. Knockdown of PbABF.D.2 decreased PbLRXA2.1 and PbLRXA2.2 expression and inhibited pollen tube growth. Notably, the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2 was repressed by self S-RNase, suggesting that self S-RNase can arrest pollen tube growth by restricting the PbABF.D.2-PbLRXA2.1/PbLRXA2.2 signal cascade. These results provide novel insight into pollen tube growth arrest by self S-RNase.
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Pyrus , Ribonucleasas , Ribonucleasas/genética , Ribonucleasas/metabolismo , Tubo Polínico/metabolismo , Pyrus/genética , Pyrus/metabolismo , Polen/genética , Citoesqueleto de Actina/metabolismoRESUMEN
Polysaccharide from Asarum sieboldii Miq (ASP) was extracted and five phosphorylation polysaccharides with different degree of substitution were obtained, namely ASPP1, ASPP2, ASPP3, ASPP4, and ASPP5 (ASPPs). The physical and chemical structure and biological activities were studied. The results suggested that the carbohydrate and protein content were reduced while uronic acid was increased after phosphorylation modification. The molecular weight of ASPPs was significantly lower than that of ASP. ASPPs were acidic heteropolysaccharides mainly composed of galacturonic acid, galactose, glucose, fructose, and arabinose. The UV-vis spectrum indicated that the polysaccharides did not contain nucleic acid or protein after modification. The Fourier transform infrared spectrum demonstrated that ASPPs contained characteristic absorption peaks of P=O and P-O-C near 1270 and 980â cm-1 . ASPPs presented a triple helix conformation, but it was not presented in ASP. The scanning electron microscopy analysis showed that the surface topography and particle structure of ASP were different after modification. Compared with ASP, ASPPs enhanced the activity to scavenge DPPH and ABTS free radicals and possessed more protective ability to DNA oxidation caused by OHâ , GSâ , and AAPH free radicals. These results suggest that chemical modification is beneficial for the exploitation and utilization of natural polysaccharides.
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Antioxidantes , Asarum , Antioxidantes/farmacología , Antioxidantes/química , Fosforilación , Polisacáridos/farmacología , Polisacáridos/química , Radicales Libres , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Polyaniline (PANI) is a promising cathode material for aqueous rechargeable zinc batteries (ARZBs), mainly benefitting from its good electrical conductivity. The high conductivity of PANI requires high doping level, yet the introduced nonactive dopants (e.g., SO4 2- ) limit the gravimetric capacity of PANI (usually < 180 mAh g-1 ). Herein, an electro-active dopant (decavanadate anion, V10 O28 6- ) is employed to fabricate the PANI cathode (PANI-V10 O28 ) for ARZBs. The doped decavanadate anion with the sub-nanometer structure can fully expose the V-based active sites, exhibiting good electrochemical activity. Due to the steric hindrance effect as well as the strong interaction between decavanadate anions and PANI chains, the active dopants are trapped in the polymer chains, demonstrating good structural and electrochemical stability. PANI-V10 O28 achieves a record-high gravimetric capacity of 355 mAh g-1 at 0.1 A g-1 , which is significantly higher than other reported PANI cathodes. Experimental results suggest that the charge storage mechanism of PANI-V10 O28 includes reversible injection/extraction of Zn(H2 O)2 Cl4 2- ions in PANI, as well as the protonation/deprotonation of V10 O28 6- . This work enriches the doping chemistry of conducting polymer and pushes the development of organic cathodes for ARZBs to a new stage.
RESUMEN
Ammonium (NH4 + ) ion as charge carrier is attracting attention in aqueous batteries. Yet, most NH4 + host materials are still limited by the relatively low capacities. Here, we fabricated a manganese phosphate (MP-20) for NH4 + ion storage. MP-20 displays a high capacity of 299.6â mAh g-1 at 1â A g-1 in ammonium acetate (NH4 Ac) electrolyte, outperforming other reported NH4 + host materials. Spectroscopy studies suggest a new NH4 + /H+ co-insertion mechanism. We surprisingly discover that the NH4 Ac electrolyte plays an important role in improving the charge storage capability of the materials. Experimental and computational results indicate acetate ions can form coordination bonds with the Mn atoms, tailoring the electronic structure of the Mn atoms and the surrounding O atoms, and therefore facilitating the NH4 + storage process. Our findings provide a new NH4 + host material and propose the important role of the electrolyte-electrode coordination effect in aqueous ammonium batteries.
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NH4 + ions as charge carriers show potential for aqueous rechargeable batteries. Studied here for the first time is the NH4 + -storage chemistry using electrodeposited manganese oxide (MnOx ). MnOx experiences morphology and phase transformations during charge/discharge in dilute ammonium acetate (NH4 Ac) electrolyte. The NH4 Ac concentration plays an important role in NH4 + storage for MnOx . The transformed MnOx with a layered structure delivers a high specific capacity (176â mAh g-1 ) at a current density of 0.5â A g-1 , and exhibits good cycling stability over 10 000â cycles in 0.5â M NH4 Ac, outperforming the state-of-the-art NH4 + hosting materials. Experimental results suggest a solid-solution behavior associated with NH4 + migration in layered MnOx . Spectroscopy studies and theoretical calculations show that the reversible NH4 + insertion/deinsertion is accompanied by hydrogen-bond formation/breaking between NH4 + and the MnOx layers. These findings provide a new prototype (i.e., layered MnOx ) for NH4 + -based energy storage and contributes to the fundamental understanding of the NH4 + -storage mechanism for metal oxides.
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Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit stone cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in fruit stone cells. In this study, we have shown that microRNA (miR) miR397a regulates fruit cell lignification by inhibiting laccase (LAC) genes that encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which is the miR397a of Chinese pear (Pyrus bretschneideri), and simultaneous silencing of three LAC genes reduced the lignin content and stone cell number in pear fruit. A single nucleotide polymorphism (SNP) identified in the promoter of the PbrmiR397a gene was found to associate with low levels of fruit lignin, after analysis of the genome sequences of sixty pear varieties. This SNP created a TCA element that responded to salicylic acid to induce gene expression as confirmed using a cell-based assay system. Furthermore, stable overexpression of PbrmiR397a in transgenic tobacco plants reduced the expression of target LAC genes and decreased the content of lignin but did not change the ratio of syringyl- and guaiacyl-lignin monomers. Consistent with reduction in lignin content, the transgenic plants showed fewer numbers of vessel elements and thinner secondary walls in the remaining elements compared to wild-type control plants. This study has advanced our understanding of the regulation of lignin biosynthesis and provided useful molecular genetic information for improving pear fruit quality.
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Frutas/crecimiento & desarrollo , Lignina/metabolismo , MicroARNs/fisiología , Pyrus/crecimiento & desarrollo , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas , Genes de Plantas/genética , Genes de Plantas/fisiología , Lignina/biosíntesis , MicroARNs/genética , Filogenia , Plantas Modificadas Genéticamente , Pyrus/genética , Pyrus/metabolismo , Análisis de Secuencia de ADN , Nicotiana/genética , Nicotiana/metabolismoRESUMEN
Circular RNAs (circRNAs) represent a class of non-coding RNAs that form covalently closed RNA circles with extensive expression and conservation in mammals. Circular RNAs regulate gene expression through acting as competitive endogenous RNAs (ceRNAs) and modulating gene transcription. Accumulating evidence supports the implication of circRNAs in a variety of human diseases, but studies of circRNA role in systemic lupus erythematosus (SLE) are lacking. The present study measured the circRNA expression profiles in T cells from patients with SLE and healthy controls with human circRNA microarray and identified 127 differentially expressed circRNAs in SLE patients. Down-regulation of hsa_circ_0045272 in SLE T cells was verified with quantitative PCR. Jurkat cells with stable hsa_circ_0045272 knockdown were generated using specific lentiviral short hairpin RNA for functional studies. Flow cytometric analysis indicated that hsa_circ_0045272 knockdown significantly up-regulated the early apoptosis of Jurkat cells. Meanwhile, ELISA showed that hsa_circ_0045272 knockdown significantly enhanced interleukin-2 production of activated Jurkat cells. Then, ceRNAs were predicted for hsa_circ_0045272 and the significant down-regulation of two mRNAs predicted as ceRNAs, NM_003466 (PAX8) and NM_015177 (DTX4), but not their corresponding proteins, was validated. Furthermore, dual luciferase reporter assay indicated binding of hsa_circ_0045272 with hsa-miR-6127. Circular RNA-mRNA co-expression networks showed the correlation of circRNAs with mRNAs and provided additional clues to circRNA functions. Our study demonstrated dysregulated circRNAs in SLE and revealed the function of hsa_circ_0045272 in negatively regulating apoptosis and interleukin-2 secretion and its potential mechanism. The implication of hsa_circ_0045272 and other abnormal circRNAs in SLE merits further investigation.
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Lupus Eritematoso Sistémico/genética , Lupus Eritematoso Sistémico/metabolismo , ARN/genética , ARN/metabolismo , Apoptosis/genética , Células Cultivadas , Perfilación de la Expresión Génica , Células HEK293 , Voluntarios Sanos , Humanos , Células Jurkat , ARN CircularRESUMEN
The present study aimed to compare polyphenols among red lettuce cultivars and identify suitable cultivars for the development and utilization of healthy vegetables. Polyphenols, mineral elements, and antioxidant activity were analyzed in the leaves of six red pigmented lettuce (Lactuca sativa L.) cultivars; thereafter, we assessed the anti-tumor effects of cultivar B-2, which displayed the highest antioxidant activity. Quadrupole-Orbitrap mass spectrometry analysis revealed four classes of polyphenols in these cultivars. The composition and contents of these metabolites varied significantly among cultivars and primarily depended on leaf color. The B-2 cultivar had the highest antioxidant potential than others because it contained the highest levels of polyphenols, especially anthocyanin, flavone, and phenolic acid; furthermore, this cultivar displayed anti-tumor effects against the human lung adenocarcinoma cell line A549, human hepatoma cell line Bel7402, human cancer colorectal adenoma cell line HCT-8, and HT-29 human colon cancer cell line. Hence, the new red-leaf lettuce cultivar B-2 has a distinct metabolite profile, with high potential for development and utilization of natural phytochemical and mineral resources in lettuces and can be used as a nutrient-dense food product.
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Antineoplásicos Fitogénicos/farmacología , Antioxidantes/farmacología , Lactuca/química , Extractos Vegetales/farmacología , Antocianinas/análisis , Antineoplásicos Fitogénicos/química , Antioxidantes/química , Células HT29 , Células Hep G2 , Humanos , Lactuca/metabolismo , Extractos Vegetales/química , Polifenoles/análisisRESUMEN
A simple, effective, and robust magnetic solid-phase extraction method was developed using magnetite/reduced graphene oxide nanoparticles as the adsorbent for the simultaneous determination of Sudan dyes (I, II, III, and IV) in foodstuffs. The magnetite/reduced graphene oxide nanoparticles were characterized by X-ray diffraction, scanning electron microscopy, and vibrating sample magnetometry. The extraction parameters including extraction time, elution solution, and elution time and volume were investigated in detail. Such magnetite/reduced graphene oxide nanoparticles based magnetic solid-phase extraction in combination with high-performance liquid chromatography and variable wavelength detection gave the detection limits of 3-6 µg/kg for Sudan I-IV in chili sauce, tomato sauce, chili powder, and chili flake samples. The recoveries were 79.6-108% at three spiked levels with the intra- and inter-day relative standard deviations of 1.2-8.6 and 4.5-9.6%, respectively. The feasibility was further performed by a comparison with commercial alumina-N. This method is suitable for the routine analysis of Sudan dyes due to its sensitivity, simplicity, and low cost.
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Compuestos Azo/análisis , Óxido Ferrosoférrico/química , Análisis de los Alimentos , Contaminación de Alimentos/análisis , Nanopartículas/química , Solanum lycopersicum/química , Extracción en Fase Sólida , Cromatografía Líquida de Alta Presión , Grafito/química , Óxidos/químicaRESUMEN
KEY MESSAGE: This research reveals the different expression patterns of anthocyanin biosynthesis enzyme genes and transcription factors in six red-skinned pear cultivars with different genetic backgrounds. Skin color is an important feature of pear fruits, with red skin generally attracting consumers. However, great differences of coloration exist in different pear cultivars. To elucidate the characteristics of pigmentation in pear cultivars with different genetic backgrounds, six cultivars, belonging to P. communis, P. pyrifolia, P. ussuriensis, P. bretschneideri, and a hybrid of P. communis × P. pyrifolia, were used to detect pigment concentrations, expressions of seven anthocyanin biosynthesis enzyme genes, and three related transcription factor genes. Results showed that the occidental pears 'Starkrimson' and 'Red Bartlett' colored at the beginning of fruit setting, but color decreased with fruit maturity; the other four cultivars showed low anthocyanin accumulations and the contents increased during fruit development, but also decreased at later stages. The expression patterns of genes encoding enzymes indicated that ANS and UFGT were decisive genes for anthocyanin biosynthesis for red-skinned pear, and their different expressions led to the coloration differences between occidental and oriental pears. The expression patterns of transcription factors indicated that the different co-expression of MYB10 and bHLH33 genes and the different expressions of WD40 are involved in the differential regulation mechanisms of anthocyanin biosynthesis and coloration pattern between occidental and oriental pears.
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Antocianinas/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/genética , Pyrus/genética , Frutas/genética , Frutas/metabolismo , Fenotipo , Pigmentación , Proteínas de Plantas/metabolismo , Pyrus/metabolismo , Especificidad de la Especie , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
Toxoplasma gondii (T. gondii)-derived heat shock protein 70 (T.g.HSP70) is a toxic protein that downregulates host defense responses against T. gondii infection. T.g.HSP70 was proven to induce fatal anaphylaxis in T. gondii infected mice through cytosolic phospholipase A2 (cPLA2) activated-platelet-activating factor (PAF) production via Toll-like receptor 4 (TLR4)-mediated signaling. In this study, we investigated the effect of arctiin (ARC; a major lignan compound of Fructus arctii) on allergic liver injury using T.g.HSP70-stimulated murine liver cell line (NCTC 1469) and a mouse model of T. gondii infection. Localized surface plasmon resonance, ELISA, western blotting, co-immunoprecipitation, and immunofluorescence were used to investigate the underlying mechanisms of action of ARC on T. gondii-induced allergic acute liver injury. The results showed that ARC suppressed the T.g.HSP70-induced allergic liver injury in a dose-dependent manner. ARC could directly bind to T.g.HSP70 or TLR4, interfering with the interaction between these two factors, and inhibiting activation of the TLR4/mitogen-activated protein kinase/nuclear factor-kappa B signaling, thereby inhibiting the overproduction of cPLA2, PAF, and interferon-γ. This result suggested that ARC ameliorates T.g.HSP70-induced allergic acute liver injury by disrupting the TLR4-mediated activation of inflammatory mediators, providing a theoretical basis for ARC therapy to improve T.g.HSP70-induced allergic liver injury.
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Toxoplasma , Toxoplasmosis , Animales , Ratones , Toxoplasma/metabolismo , Receptor Toll-Like 4/metabolismo , Factor de Activación Plaquetaria , Toxoplasmosis/tratamiento farmacológico , Proteínas HSP70 de Choque Térmico/metabolismo , Hígado/metabolismo , Fosfolipasas/metabolismoRESUMEN
BACKGROUND: This study aims to explore the efficacy of Relaxation Response Meditation Training (RRMT) on elderly individuals with different levels of vividness of visual imagery. METHODS: In this randomized controlled, double-blind, multi-center clinical trial, we recruited a total of 136 elderly individuals who were over 60 years with nonorganic sleep disorders to participate in a 4-week RRMT intervention from October 2020 to October 2022. The intervention occurred twice a week, totaling eight times. These individuals were divided into high and low groups based on the vividness of visual imagery, and then randomly assigned to either the control or intervention groups, as follows: low-visualizers intervention group (LI group); low-visualizers control group (LC group); high-visualizers intervention group (HI group); high-visualizers control group (HC group). Their social and psychological parameters were assessed before and after the intervention by the Pittsburgh Sleep Quality Index (PSQI), the Revised Piper's fatigue scale (RPFS), General well-being scale (GWB), and Satisfaction rating. The alpha waves of patients were also collected through electroencephalogram to assess their level of relaxation. RESULTS: Compared to the LI group, the HI group had a greater reduction rate in the PSQI score [25.2 % (18.8 % to 31.7 %), P < 0â001], shorter sleep latency (P = 0.001), lower frequency of sleep medication (P < 0.001), lower PSQI scores (P < 0.001), and higher GWB scores (P < 0.001). There were significant differences in all indicators in the HI group vs. HC group and in the LI group vs. LC group. In the first five relaxation training sessions, there was no statistically significant difference in the proportion of α waves between the LI group and the LC group; however, from the sixth session onward, we observed a statistically significant difference (t = 2.86, P = 0.019)ï¼while The HI group and HC group showing significant differences in the first relaxation training session (t = 4.464, P < 0.001). There was a statistically significant difference in subjective satisfaction between the intervention group and the control group (x2 = 49.605, P < 0.001). CONCLUSION: In this study, we found that most elderly people benefitted from RRMT regardless of their vividness of visual imagery. However, low-visualizers experienced slower and less effective results, so these patients may benefit more from alternative approaches.
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Meditación , Terapia por Relajación , Trastornos del Inicio y del Mantenimiento del Sueño , Humanos , Método Doble Ciego , Masculino , Femenino , Anciano , Meditación/métodos , Trastornos del Inicio y del Mantenimiento del Sueño/terapia , Trastornos del Inicio y del Mantenimiento del Sueño/psicología , Terapia por Relajación/métodos , Persona de Mediana Edad , Imágenes en Psicoterapia/métodos , Resultado del Tratamiento , Calidad del SueñoRESUMEN
This study aimed to investigate the role of autophagy, ferroptosis, and pyroptosis in the antitumour mechanism of harmine (Har) and its crosstalk in ovarian cancer. By transmission electron microscopy, we found that compared with those in the control group, the cytoplasm of human ovarian cancer cells (SKOV3) treated with Har showed increased numbers of autophagic vesicles, decreased intracellular mitochondrial volume, increased bilayer membrane density, and decreased cristae. Western blot, immunofluorescence, and monodasylcadaverine (MDC) staining all suggested that Har promoted autophagy in SKOV3 cells. LY294002 and siFOXO3 rescued the inhibition of the PI3K/AKT/mTOR/FOXO3 signalling pathway and the promotion of autophagy by Har. Additionally, the levels of ferroptosis- and pyroptosis-related proteins and the levels of Fe2+ , glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD) suggested that Har promoted ferroptosis and pyroptosis in SKOV3 cells. Interestingly, pretreatment with chloroquine (CQ), erastin, rapamycin (Rap), or ferrostatin-1 (Fer-1) increased or reversed the ferroptosis and pyroptosis promoted by Har, respectively. In vivo, the volume of tumours in the Har group was decreased, and immunohistochemistry revealed decreased levels of Ki-67 and GPX4 and increased levels of ATG5 and NARL3. In conclusion, Har exerts its anti-ovarian cancer effect not only by promoting autophagy by regulating the PI3K/AKT/mTOR/FOXO3 signalling pathway but also by promoting ferroptosis and pyroptosis. Additionally, there is complex crosstalk between autophagy, ferroptosis, and pyroptosis in ovarian cancer.
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Ferroptosis , Neoplasias Ováricas , Femenino , Humanos , Piroptosis , Harmina/farmacología , Proteínas Proto-Oncogénicas c-akt/metabolismo , Fosfatidilinositol 3-Quinasas , Serina-Treonina Quinasas TOR/metabolismo , Neoplasias Ováricas/tratamiento farmacológico , Neoplasias Ováricas/metabolismo , AutofagiaRESUMEN
OBJECTIVE: To evaluate the effects of hypothermia on cerebral edema and metabolism, a porcine model of cardiac arrest was assessed by magnetic resonance imaging during the first 72 hours after restoration of spontaneous circulation (ROSC). METHODS: Ventricular fibrillation was induced in 33 pigs. After 8 minutes of untreated ventricular fibrillation, 30:2 cardiopulmonary resuscitation was performed. After ROSC, 30 survival animals were randomly divided into normothermia group (n = 15) and hypothermia group (n = 15). The hypothermia group immediately received endovascular cooling to regulate temperature to 33°C, which was maintained for 12 hours, followed by passive rewarming at 0.5°C/h to 37°C. Diffusion-weighted imaging and (1)hydrogen proton magnetic resonance spectroscopy were acquired for each group at 6, 12, 24, and 72 hours after ROSC. RESULTS: Compared with the normothermia group, the hypothermia group exhibited a higher 72-hour survival (73.3% vs. 33.3%, P = .028) and a superior neurological deficit score (P = .031). Cerebral injury was found in both groups, but a lesser decrease in the apparent diffusion coefficient and N-acetyl aspartate/creatinine (P < .05) and a greater increase in choline/creatinine (P < .05) were found in the hypothermia group. CONCLUSIONS: Magnetic resonance imaging could effectively detect the dynamic trend of cerebral injury in a porcine model of cardiac arrest within the first 72 hours after ROSC. Hypothermia produced a protective effect on neurological function by reducing brain edema and formation of adverse metabolites.
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Edema Encefálico/metabolismo , Edema Encefálico/prevención & control , Reanimación Cardiopulmonar/métodos , Paro Cardíaco/metabolismo , Paro Cardíaco/terapia , Hipotermia Inducida , Imagen por Resonancia Magnética/métodos , Animales , Edema Encefálico/fisiopatología , Paro Cardíaco/fisiopatología , Hemodinámica , Masculino , Distribución Aleatoria , Estadísticas no Paramétricas , Tasa de Supervivencia , PorcinosRESUMEN
As a typical solitary animal, adult giant pandas rely on chemical signals (sex pheromones) to transmit reproductive information during oestrous. Although researchers have confirmed that the gut microbiota is related to the emission and reception of sex pheromones, there is no clear correlation between the gut microbes and the synthesis of sex pheromone of giant pandas, that is, which gut microbes and microbial metabolites are participate in the synthesis of giant panda's sex pheromone. As a mirror of gut microbiota, fecal microbiota can reflect the composition of gut microbiota and its interaction with host to some extent. The purpose of this study is to explore how the gut microbes affect the synthesis of sex pheromones in captive giant pandas by combining analysis of the fecal microbiome and metabolomics. The results of correlation and microbial function analysis show that intestinal microorganisms such as Veillonellaceae and Lactobacillilaceae are associated with the synthesis of short chain fatty acid (acetic acid) and volatile ester metabolites, such as 1-butanol, 3-methyl, acetate, acetic acid, hexyl ester and 3-hexen-1-ol, acetate, (Z). In summary, based on this study, we believe that volatile metabolites such as fecal acetate participate in the process of mate preference of captive giant pandas and affect their expression of natural mating behavior. The possible mechanism is that the gut microbes can promote the synthesis of key chemical signaling substances in perianal glands through mediated intermediate fecal metabolites, thus affecting the normal information exchange between giant pandas individuals. The results of this study have greatly enriched our understanding of gut microbes regulating the synthesis of sex pheromones in giant pandas.
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Conservation of genetic resources is an important way to protect endangered species. At present, mesenchymal stem cells (MSCs) have been isolated from the bone marrow and umbilical cords of giant pandas. However, the types and quantities of preserved cell resources were rare and limited, and none of MSCs was derived from female reproductive organs. Here, we first isolated MSCs from the endometrium of giant panda. These cells showed fibroblast morphology and expressed Sox2, Klf4, Thy1, CD73, CD105, CD44, CD49f, and CD105. Endometrium mesenchymal stem cells (eMSCs) of giant panda could induce differentiation into three germ layers in vitro. RNA-seq analysis showed that 833 genes were upregulated and 716 genes were downregulated in eMSCs compared with skin fibroblast cells. The results of GO and the KEGG analysis of differentially expressed genes (DEGs) were mainly focused on transporter activity, signal transducer activity, pathways regulating pluripotency of stem cells, MAPK signaling pathway, and PI3K-Akt signaling pathway. The genes PLCG2, FRK, JAK3, LYN, PIK3CB, JAK2, CBLB, and MET were identified as hub genes by PPI network analysis. In addition, the exosomes of eMSCs were also isolated and identified. The average diameter of exosomes was 74.26 ± 13.75 nm and highly expressed TSG101 and CD9 but did not express CALNEXIN. A total of 277 miRNAs were detected in the exosomes; the highest expression of miRNA was the has-miR-21-5p. A total of 14461 target genes of the whole miRNAs were predicted and proceeded with functional analysis. In conclusion, we successfully isolated and characterized the giant panda eMSCs and their exosomes, and analyzed their functions through bioinformatics techniques. It not only enriched the conservation types of giant panda cell resources and promoted the protection of genetic diversity, but also laid a foundation for the application of eMSCs and exosomes in the disease treatment of giant pandas.
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Exosomas , Células Madre Mesenquimatosas , MicroARNs , Ursidae , Femenino , Animales , Ursidae/genética , Exosomas/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Células Madre Mesenquimatosas/metabolismo , MicroARNs/genética , Endometrio/metabolismoRESUMEN
Mate choice was an important factor affecting the success rate of natural mating of captive giant pandas. The influencing factors and mechanisms of the mate preference of captive giant pandas were still unclear, and it was speculated that they might be related to the psychological stress caused by the long-term confined environment restricting their free choice of physiological needs. In order to test this hypothesis, this work explored the urinary metabolites of 6 adult captive male giant pandas during breeding period. Differences in metabolite levels in giant panda urine samples were analyzed via Ultra High Performance LC-MS (UHPLC/-MS) comparing preservation to loss of natural reproductive capacity and success to failure of mating choice, trying to understand the psychological feelings of captive giant pandas in the process of mate choice from the perspective of all metabolites and related biochemical pathways, and fully revealed the mechanism of decline of their natural reproductive ability. The analysis results indicated that the loss of natural mating behavior of adult captive male giant pandas might be related to the disorder of tryptophan metabolism pathway and inhibition of arginine synthesis; the reason for the failure of mating choice caused by decreased libido might be related to the temporary decrease of androgen contents caused by the down-regulated of TCA cycle function and galactose metabolic pathway. These findings not only provide that adult male giant pandas in captivity do have psychological frustration caused by the inability to freely choose their favorite mate or failure of mating preference, but also showed that the changes in stress-related metabolic pathways caused by psychological frustration were an important reason for the decline of natural mating behavior of adult captive male giant pandas.
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Ursidae , Andrógenos/metabolismo , Animales , Masculino , Espectrometría de Masas , Metabolómica , Reproducción , Ursidae/metabolismo , Ursidae/orinaRESUMEN
Metal-organic frameworks have great potential in the field of proton conducting materials and fluorescent probes due to their structural tunability and designability. A novel water-stable metal organic framework material [Cd2(Hdpb)(H2O)3] (Cd-MOF) was synthesized based on H5dpb (H5dpb = 3,5-diphosphonobenzoic acid) and Cd2+ ions. Cd2+ ions are connected with phosphonates and carboxyl groups of H5dpb to form an infinitely extended 1D chain, which is further connected by the Hdpb4- ligand and coordinated water to form a three-dimensional network structure. There are hydrogen bond networks in the 3D structure of the Cd-MOF, which are favorable for proton transfer, achieving its maximum proton conductivity of 2.97 × 10-3 S cm-1 at 338 K and 98% relative humidity (RH). To realize its application in fuel cells, the Cd-MOF was introduced into the chitosan (CS) matrix, and a series of composite membranes (Cd-MOF@CS-X) with high proton conductivity were obtained. The results of AC impedance show that the proton conductivity of Cd-MOF@CS-5 reaches 3.55 × 10-1 S cm-1 at 358 K and 98% RH, which is comparable to the highest values reported for MOF-polymer complexes. Moreover, the Cd-MOF can be used as a selective fluorescent probe for pyridine detection, and its detection limit can reach 1.0 × 10-6 M. A bifunctional MOF with proton conduction and pyridine recognition is reported for the first time, and has important reference value for the practical application of functional MOFs in both electrochemical and luminescence sensing.
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Estructuras Metalorgánicas , Protones , Cadmio , Fluorescencia , Colorantes Fluorescentes , Estructuras Metalorgánicas/química , Piridinas , AguaRESUMEN
Aqueous rechargeable Zn-manganese dioxide (Zn-MnO2) hybrid batteries based on dissolution-deposition mechanisms exhibit ultrahigh capacities and energy densities due to the two-electron transformation between MnO2/Mn2+. However, the reported Zn-MnO2 hybrid batteries usually use strongly acidic and/or alkaline electrolytes, which may lead to environmental hazards and corrosion issues of the Zn anodes. Herein, we propose a new Zn-MnO2 hybrid battery by adding Al3+ into the sulfate-based electrolyte. The hybrid battery undergoes reversible MnO2/Mn2+ transformation and exhibits good electrochemical performances, such as a high discharge capacity of 564.7 mAh g-1 with a discharge plateau of 1.65 V, an energy density of 520.8 Wh kg-1, and good cycle life without capacity decay upon 2000 cycles. Experimental results and theoretical calculation suggest that the aquo Al3+ with Brønsted weak acid nature can act as the proton-donor reservoir to maintain the electrolyte acidity near the electrode surface and prevent the formation of Zn4(OH)6(SO4)·0.5H2O during discharging. In addition, Al3+ doping during charging introduces oxygen vacancies in the oxide structure and weakens the Mn-O bond, which facilitates the dissolution reaction during discharge. The mechanistic investigation discloses the important role of Al3+ in the electrolyte, providing a new fundamental understanding of the promising aqueous Zn-MnO2 batteries.
RESUMEN
The decline in natural mating behavior is the primary reason underlying in the poor population growth of captive giant pandas. However, the influencing factors and underlying mechanisms remain unclear to data. It is speculated that the decline in natural mating behavior could be related to the psychological stress caused by captivity, which restricts their free choice of mates. In order to test this hypothesis, we performed urinary metabolomics analysis using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC/-MS) combined with 16S rDNA sequencing for exploring the physiological mechanism underlying the decline in the natural mating behavior of captive giant panda. The results demonstrated that the decline in mating ability could be related to abnormalities in arginine biosynthesis and neurotransmitter synthesis. Additionally, the relative abundance of bacteria from the Firmicutes, Proteobacteria, and Actinobacteria phyla and the Acinetobacter, Weissella, and Pseudomonas genus was significantly reduced in the group with low natural mating behavior. These findings imply that the inhibition of arginine synthesis induced by environmental changes could be related to the poor libido and failure of mate selection in captive giant pandas during the breeding period. The results also demonstrate the relationship between the altered urinary microbes and metabolites related to arginine and neurotransmitter synthesis. These findings may aid in understanding the mechanism underlying environment-induced mate selection in captive giant pandas and propose a novel strategy for determining the sexual desire of giant pandas based on urinary microbes. The method would be of great significance in improving the natural reproductive success rate of captive giant pandas.