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OBJECTIVES: Previous report shows that corneal spot size is an important influence factor on damage threshold induced by supercontinumm (SC) source. However, damage thresholds were determined for the spot size of only 0.37 mm due to the low output of the employed SC source at that time. The objectives of this study are to determine the lowest possible corneal damage threshold at a large corneal spot size using a more powerful SC source and provide data for the future possible refinements of laser safety standards. MATERIALS AND METHODS: A series of experiments was conducted in the New Zealand white rabbit model to determine the corneal damage threshold induced by a 900-2000 nm SC source, with corneal 1/e beam diameter of about 1.2 mm. Slit-lamp biomicroscope was employed to reveal the corneal damage characteristics. By employing the action spectra determined through the analysis of current laser safety guidelines and standards, the effective damage threshold could be calculated. RESULTS: The determined damage threshold given in terms of the peak radiant exposure for the exposure duration of 0.14 s was 44.3 J/cm2 . At threshold level, corneal damages involved the epithelium and the shallower stroma. The safety factor between the effective damage threshold and the corresponding maximum permissible exposure (MPE) was about 45. CONCLUSIONS: Present corneal MPEs in the wavelength range of 700-1200 nm may be conservative and corneal damage thresholds for the infrared A wavelengths should be determined in future studies.
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We experimentally studied the supercontinuum induced by femtosecond filamentation in different liquid media. Using a Mach-Zehnder interferometer, we determined the relative filamentation thresholds (Pth) of these media. Research has shown that the value of the filamentation threshold is greater than that of Pcr (critical power for self-focusing), which can mainly be attributed to the strong dispersion effect. Changing the focal length of the focusing lens affects filamentation dynamics, thereby affecting the measured results regarding the filamentation threshold. With shorter focal lengths, the linear focusing (i.e., geometrical focusing) regime dominates, and the measured values of Pth for different liquid media are almost the same; as the focal length becomes larger, self-focusing starts to play a role, making the values of Pth for different media different from each other. This study presents an efficient method for investigating the femtosecond filamentation phenomenon in liquid media, helpful to provide further insights into the physical mechanism of supercontinuum generation via femtosecond filamentation in liquid media.
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The elastic light-scatter (ELS) technique, which detects and discriminates microbial organisms based on the light-scatter pattern of their colonies, has demonstrated excellent classification accuracy in pathogen screening tasks. The implementation of the multispectral approach has brought further advantages and motivated the design and validation of a hyperspectral elastic light-scatter phenotyping instrument (HESPI). The newly developed instrument consists of a supercontinuum (SC) laser and an acousto-optic tunable filter (AOTF). The use of these two components provided a broad spectrum of excitation light and a rapid selection of the wavelength of interest, which enables the collection of multiple spectral patterns for each colony instead of relying on single band analysis. The performance was validated by classifying microflora of green-leafed vegetables using the hyperspectral ELS patterns of the bacterial colonies. The accuracy ranged from 88.7% to 93.2% when the classification was performed with the scattering pattern created at a wavelength within the 473-709 nm region. When all of the hyperspectral ELS patterns were used, owing to the vastly increased size of the data, feature reduction and selection algorithms were utilized to enhance the robustness and ultimately lessen the complexity of the data collection. A new classification model with the feature reduction process improved the overall classification rate to 95.9%.
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Bacterias , Elasticidad , Luz , Fenómenos Fisiológicos Bacterianos , AlgoritmosRESUMEN
We demonstrate the successful implementation of an artificial neural network (ANN) to eliminate detrimental spectral shifts imposed in the measurement of laser absorption spectrometers (LASs). Since LASs rely on the analysis of the spectral characteristics of biological and chemical molecules, their accuracy and precision is especially prone to the presence of unwanted spectral shift in the measured molecular absorption spectrum over the reference spectrum. In this paper, an ANN was applied to a scanning grating-based mid-infrared trace gas sensing system, which suffers from temperature-induced spectral shifts. Using the HITRAN database, we generated synthetic gas absorbance spectra with random spectral shifts for training and validation. The ANN was trained with these synthetic spectra to identify the occurrence of spectral shifts. Our experimental verification unambiguously proves that such an ANN can be an excellent tool to accurately retrieve the gas concentration from imprecise or distorted spectra of gas absorption. Due to the global shift of the measured gas absorption spectrum, the accuracy of the retrieved gas concentration using a typical least-mean-squares fitting algorithm was considerably degraded by 40.3%. However, when the gas concentration of the same measurement dataset was predicted by the proposed multilayer perceptron network, the sensing accuracy significantly improved by reducing the error to less than ±1% while preserving the sensing sensitivity.
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In this paper, we design a silica-cladded Germania-doped ring-core fiber (RCF) that supports orbital angular momentum (OAM) modes. By optimizing the fiber structure parameters, the RCF possesses a near-zero flat dispersion with a total variation of <±30 ps/nm/km over 1770 nm bandwidth from 1040 to 2810 nm for the OAM1,1 mode. A beyond-two-octave supercontinuum spectrum of the OAM1,1 mode is generated numerically by launching a 40 fs 120 kW pulse train centered at 1400 nm into a 12 cm long designed 50 mol% Ge-doped fiber, which covers 2130 nm bandwidth from 630 nm to 2760 nm at −40 dB of power level. This design can serve as an efficient way to extend the spectral coverage of beams carrying OAM modes for various applications.
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High-intensity (â¼1 TW/cm2 and higher) region formed in the propagation of â¼60 GW, 90 fs Ti:Sapphire laser pulse on a â¼100 m path in air spans for several tens of meters and includes a plasma filament and a postfilament light channel. The intensity in this extended region is high enough to generate an infrared supercontinuum wing and to initiate laser-induced discharge in the gap between the electrodes. In the experiment and simulations, we delay the high-intensity region along the propagation direction by inserting metal-wire meshes with square cells at the laser system output. We identify the presence of a high-intensity region from the clean-spatial-mode distributions, appearance of the infrared supercontinuum wing, and occurrence of the laser-induced discharge. In the case of free propagation (without any meshes), the onset of the high-intensity zone is at 40-52 m from the laser system output with â¼30 m extension. Insertion of the mesh with 3 mm cells delays the beginning of the high-intensity region to 49-68 m with the same â¼30 m extension. A decrease in the cell size to 1 mm leads to both delay and shrinking of the high-intensity zone to 71-73 m and 6 m, respectively. Three-dimensional simulations in space confirm the mesh-induced delay of the high-intensity zone as the cell size decreases.
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BACKGROUND AND OBJECTIVES: Widespread applications of supercontinuum (SC) source lead to the possibility of ocular damages. However, the corneal damage effects induced by SC have not been explored before. The objectives of this study are to determine the rabbit corneal injury threshold for SC radiation and to examine whether the existing safety guidelines and standards are suitable for the hazard evaluation of this new kind of light source. STUDY DESIGN/MATERIALS AND METHODS: A series of experiments was conducted in the New Zealand white rabbit model to determine the corneal damage thresholds induced by a 770-2,500 nm SC source, with a corneal 1/e beam diameter of 0.37 mm. Through slit-lamp biomicroscope, optical coherence tomography (OCT), and histopathology the corneal damage characteristics at the threshold level were revealed. By employing the action spectra determined through the analysis of safety guidelines and standards, the damage thresholds for SC source could be compared with the corresponding exposure limits. RESULTS: The determined damage thresholds given in terms of the peak radiant exposure for exposure durations of 2.0 and 10.0 seconds were 2.1 × 103 and 7.4 × 103 J/cm2 , respectively. At threshold level, corneal damages involved the epithelium and the shallower stroma, and no obvious changes could be found in the deep stroma, Descemet's membrane, and endothelium. CONCLUSIONS: The exposure limits for the anterior parts of the eye in the wavelength range of 700-1,200 nm are overly conservative. The obtained results contribute to the knowledge base for the hazard evaluation of SC source. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
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Córnea/efectos de la radiación , Lesiones de la Cornea/etiología , Rayos Láser/efectos adversos , Animales , Lesiones de la Cornea/diagnóstico por imagen , Modelos Animales de Enfermedad , Relación Dosis-Respuesta en la Radiación , Femenino , Masculino , Conejos , Tomografía de Coherencia ÓpticaRESUMEN
Shaping is very necessary in order to obtain a wide and flat supercontinuum (SC). Via numerical simulations, we accurately demonstrated shaping the SC using the fiber cascading method to significantly increase the width as well as the flatness of the spectrum in silica photonic crystal fiber (PCF). The cascaded PCF contains two segments, each of which has dual zero-dispersion frequencies (ZDFs). The spectral range of the SC can be expanded tremendously by tuning the spacing between the two ZDFs of the first segmented cascaded PCF. Increasing the pump power generates more solitons at the red edge, which accelerates solitons trapping and improves the spectral flatness of the blue edge. Furthermore, cascading the second segmented PCF by choosing appropriate fiber parameters ensures the flatness of the red end of SC. Therefore, a cost-effective alternative method for broad and flat supercontinuum generation in the near-infrared range is proposed here, which can be implemented easily in any photonics laboratory, where dual ZDFs PCFs are commonly found.
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In this paper, a gas sensing system based on a conventional absorption technique using a single-mode-fiber supercontinuum source (SMF-SC) is presented. The SC source was implemented by channeling pulses from a microchip laser into a one kilometer long single-mode fiber (SMF), obtaining a flat high-spectrum with a bandwidth of up to 350 nm in the region from 1350 to 1700 nm, and high stability in power and wavelength. The supercontinuum radiation was used for simultaneously sensing water vapor and acetylene gas in the regions from 1350 to 1420 nm and 1510 to 1540 nm, respectively. The experimental results show that the absorption peaks of acetylene have a maximum depth of approximately 30 dB and contain about 60 strong lines in the R and P branches, demonstrating a high sensitivity of the sensing setup to acetylene. Finally, to verify the experimental results, the experimental spectra are compared to simulations obtained from the Hitran database. This shows that the implemented system can be used to develop sensors for applications in broadband absorption spectroscopy and as a low-cost absorption spectrophotometer of multiple gases.
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An active hyperspectral sensor (AHS) was developed for target detection and classification applications. AHS measures light scattered from a target, illuminated by a broadband near-infrared supercontinuum (SC) light source. Spectral discrimination is based on a voltage-tunable MEMS Fabry-Pérot Interferometer (FPI). The broadband light is filtered by the FPI prior to transmitting, allowing for a high spectral-power density within the eye-safety limits. The approach also allows for a cost-efficient correction of the SC instability, employing a non-dispersive reference detector. A precision of 0.1% and long-term stability better than 0.5% were demonstrated in laboratory tests. The prototype was mounted on a car for field measurements. Several road types and objects were distinguished based on the spectral response of the sensor targeted in front of the car.
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We present a fully integrated and transportable multi-species trace gas sensor based on a mid-infrared (MIR) supercontinuum light source. The high brightness (surpassing synchrotron) and ultra-broad spectral bandwidth (2-4 µm) of this light source allows simultaneous detection of multiple broadband absorbing gas species. High sensitivity in the sub-ppmv level has been achieved by utilizing an astigmatic multipass cell. A grating-based spectrometer at a scanning rate of 20 Hz is developed employing a balanced detection scheme. A multi-component global fitting algorithm is implemented into a central LabVIEW program to perform real-time data analysis. The obtained concentration values are validated by the standard gas chromatography mass spectrometry (GC-MS) method. Field application of the sensor for quality control of stored fruits at a small scale is demonstrated, involving the detection of ethylene, ethanol, ethyl acetate, acetaldehyde, methanol, acetone, and water simultaneously. The sensor also shows promising potentials for other applications, such as environmental monitoring and biomedical research.
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Hyperspectral LiDAR (HSL) technology can obtain spectral and ranging information from targets by processing the recorded waveforms and measuring the time of flight (ToF). With the development of the supercontinuum laser (SCL), it is technically easier to develop an active hyperspectral LiDAR system that can simultaneously collect both spatial information and extensive spectral information from targets. Compared with traditional LiDAR technology, which can only obtain range and intensity information at the selected spectral wavelengths, HSL detection technology has demonstrated its potential and adaptability for various quantitative applications from its spectrally resolved waveforms. However, with most previous HSLs, the collected spectral information is discrete, and such information might be insufficient and restrict the further applicability of the HSLs. In this paper, a tunable HSL technology using an acousto-optic tunable filter (AOTF) as a spectroscopic device was proposed, designed, and tested to address this issue. Both the general range precision and the accuracy of the spectral measurement were evaluated. By tuning the spectroscopic device in the time dimension, the proposed AOTF-HSL could achieve backscattered echo with continuous coverage of the full spectrum of 500-1000 nm, which had the unique characteristics of a continuous spectrum in the visible and near infrared (VNIR) regions with 10 nm spectral resolution. Yellow and green leaves from four plants (aloe, dracaena, balata, and radermachera) were measured using the AOTF-HSL to assess its feasibility in agriculture application. The spectral profiles measured by a standard spectrometer (SVC© HR-1024) were used as a reference for evaluating the measurements of the AOTF-HSL. The difference between the spectral measurements collected from active and passive instruments was minor. The comparison results show that the AOTF-based consecutive and high spectral resolution HSL was effective for this application.
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The identity of a fluorophore can be ambiguous if other fluorophores or nonspecific fluorescent impurities have overlapping emission spectra. The presence of overlapping spectra makes it difficult to differentiate fluorescent species using discrete detection channels and unmixing of spectra. The unique absorption and emission signatures of fluorophores provide an opportunity for spectroscopic identification. However, absorption spectroscopy may be affected by scattering, whereas fluorescence emission spectroscopy suffers from signal loss by gratings or other dispersive optics. Photoluminescence excitation spectra, where excitation is varied and emission is detected at a fixed wavelength, allows hyperspectral imaging with a single emission filter for high signal-to-background ratio without any moving optics on the emission side. We report a high throughput method for measuring the photoluminescence excitation spectra of individual fluorophores using a tunable supercontinuum laser and prism-type total internal reflection fluorescence microscope. We used the system to measure and sort the photoluminescence excitation spectra of individual Alexa dyes, fluorescent nanodiamonds (FNDs), and fluorescent polystyrene beads. We used a Gaussian mixture model with maximum likelihood estimation to objectively separate the spectra. Finally, we spectroscopically identified different species of fluorescent nanodiamonds with overlapping spectra and characterized the heterogeneity of fluorescent nanodiamonds of varying size.
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In this work a photonic crystal fiber made of a heavy metal oxide glass with optimized dispersion profile is proposed for supercontinuum generation in a broad range of wavelengths in the near-infrared, when pumped by a mode-locked fiber-based laser. The fiber is modelled and optimal geometrical parameters are selected to achieve flat and low dispersion in the anomalous regime. Supercontinuum generation in the range of 0.76â»2.40 µm, within the dynamics of 30 dB, when pumped at 1.56 µm with 400 fsâ»long pulses and an average power 660 mW is possible. The applicability of such fibers is also discussed.
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Incoherent broadband cavity-enhanced absorption spectroscopy (IBBCEAS) is of importance for gas detection in environmental monitoring. This review summarizes the unique properties, development and recent progress of the IBBCEAS technique. Principle of IBBCEAS for gas sensing is described, and the development of IBBCEAS from the perspective of system structure is elaborated, including light source, cavity and detection scheme. Performances of the reported IBBCEAS sensor system in laboratory and field measurements are reported. Potential applications of this technique are discussed.
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Recent advances in nanoscale bioreplication processes present the potential for novel basic and applied research into organismal behavioral processes. Insect behavior potentially could be affected by physical features existing at the nanoscale level. We used nano-bioreplicated visual decoys of female emerald ash borer beetles (Agrilus planipennis) to evoke stereotypical mate-finding behavior, whereby males fly to and alight on the decoys as they would on real females. Using an industrially scalable nanomolding process, we replicated and evaluated the importance of two features of the outer cuticular surface of the beetle's wings: structural interference coloration of the elytra by multilayering of the epicuticle and fine-scale surface features consisting of spicules and spines that scatter light into intense strands. Two types of decoys that lacked one or both of these elements were fabricated, one type nano-bioreplicated and the other 3D-printed with no bioreplicated surface nanostructural elements. Both types were colored with green paint. The light-scattering properties of the nano-bioreplicated surfaces were verified by shining a white laser on the decoys in a dark room and projecting the scattering pattern onto a white surface. Regardless of the coloration mechanism, the nano-bioreplicated decoys evoked the complete attraction and landing sequence of Agrilus males. In contrast, males made brief flying approaches toward the decoys without nanostructured features, but diverted away before alighting on them. The nano-bioreplicated decoys were also electroconductive, a feature used on traps such that beetles alighting onto them were stunned, killed, and collected.
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Escarabajos/fisiología , Conducta Sexual Animal/fisiología , Comunicación Animal , Animales , Materiales Biomiméticos , Color , Femenino , Vuelo Animal , Masculino , Modelos Biológicos , Nanoestructuras , Nanotecnología , Conducta Estereotipada , Visión OcularRESUMEN
The supercontinuum generated exclusively in the normal dispersion regime of a nonlinear fiber is widely believed to possess low optical noise and high spectral coherence. The recent development of flattened all-normal dispersion fibers has been motivated by this belief to construct a general-purpose broadband coherent optical source. Somewhat surprisingly, we identify a large short-term polarization noise in this type of supercontinuum generation that has been masked by the total-intensity measurement in the past, but can be easily detected by filtering the supercontinuum with a linear polarizer. Fortunately, this hidden intrinsic noise and the accompanied spectral decoherence can be effectively suppressed by using a polarization-maintaining all-normal dispersion fiber. A polarization-maintaining coherent supercontinuum laser is thus built with a broad bandwidth (780-1300 nm) and high spectral power (~1 mW/nm).
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This review is aimed at presenting the state-of-the-art of time domain (TD) functional near-infrared spectroscopy (fNIRS). We first introduce the physical principles, the basics of modeling and data analysis. Basic instrumentation components (light sources, detection techniques, and delivery and collection systems) of a TD fNIRS system are described. A survey of past, existing and next generation TD fNIRS systems used for research and clinical studies is presented. Performance assessment of TD fNIRS systems and standardization issues are also discussed. Main strengths and weakness of TD fNIRS are highlighted, also in comparison with continuous wave (CW) fNIRS. Issues like quantification of the hemodynamic response, penetration depth, depth selectivity, spatial resolution and contrast-to-noise ratio are critically examined, with the help of experimental results performed on phantoms or in vivo. Finally we give an account on the technological developments that would pave the way for a broader use of TD fNIRS in the neuroimaging community.
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Mapeo Encefálico/métodos , Neuroimagen Funcional/métodos , Espectroscopía Infrarroja Corta/métodos , Mapeo Encefálico/instrumentación , Interpretación Estadística de Datos , Diagnóstico por Imagen , Neuroimagen Funcional/instrumentación , Humanos , Espectroscopía Infrarroja Corta/instrumentaciónRESUMEN
Supercontinuum generation (SCG) from silica-based photonic crystal fibers (PCFs) is of highly technological significance from microscopy to metrology, but has been hindered by silica's relatively low intrinsic optical nonlinearity. The prevailing approaches of filling PCF with nonlinear gases or liquids can endow fibre with enhanced optical nonlinearity and boosted SCG efficiency, yet these hybrids are easily plagued by fusion complexity, environmental incompatibility or transmission mode instability. Here this work presents a strategy of embedding solid-state 2D MoS2 atomic layers into the air-holes of PCF to efficiently enhance SCG. This work demonstrates a 4.8 times enhancement of the nonlinear coefficient and a 70% reduction of the threshold power for SCG with one octave spanning in the MoS2-PCF hybrid. Furthermore, this work finds that the SCG enhancement is highly layer-dependent, which only manifests for a real 2D regime within the thickness of five atomic layers. Theoretical calculations reveal that the critical thickness arises from the trade-off among the layer-dependent enhancement of the nonlinear coefficient, leakage of fundamental mode and redshift of zero-dispersion wavelength. This work provides significant advances toward efficient SCG, and highlights the importance of matching an appropriate atomic layer number in the design of functional 2D material optical fibers.
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We propose a single material microstructured optical fiber with more than five octave spanning supercontinuum generation. Due to using single material, compatibility checking between core and cladding material need not required. Moreover, the material is such chosen that optical transmission range is quite high in comparison to others. The fiber is designed in COMSOL platform and according to finite element method (FEM), numerical analysis is accomplished. Dispersion, confinement loss, nonlinearity etc. are investigated and exposed for a wavelength range of 2000-3000 nm. The proposed fiber reveals zero dispersion at 2200 nm, nonlinearity of 14,370 W-1km-1 and confinement loss around 10-9 order in dB/m. Finally, supercontinuum generation has been investigated inside the structure and obtained high coherence that span (from 1500 nm to 34,500 nm) over five octave. The generated spectrum fulfills the requirements for being applicable in frequency metrology, fiber laser and biophotonics. With the best of our knowing, such coherent and widened supercontinuua for single material fiber is yet to be proposed.