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Nucleic Acids Res ; 49(16): 9526-9538, 2021 09 20.
Artículo en Inglés | MEDLINE | ID: mdl-33836081

RESUMEN

The use of a small molecule compound to reduce toxic repeat RNA transcripts or their translated aberrant proteins to target repeat-expanded RNA/DNA with a G4C2 motif is a promising strategy to treat C9orf72-linked disorders. In this study, the crystal structures of DNA and RNA-DNA hybrid duplexes with the -GGGCCG- region as a G4C2 repeat motif were solved. Unusual groove widening and sharper bending of the G4C2 DNA duplex A-DNA conformation with B-form characteristics inside was observed. The G4C2 RNA-DNA hybrid duplex adopts a more typical rigid A form structure. Detailed structural analysis revealed that the G4C2 repeat motif of the DNA duplex exhibits a hydration shell and greater flexibility and serves as a 'hot-spot' for binding of the anthracene-based nickel complex, NiII(Chro)2 (Chro = Chromomycin A3). In addition to the original GGCC recognition site, NiII(Chro)2 has extended specificity and binds the flanked G:C base pairs of the GGCC core, resulting in minor groove contraction and straightening of the DNA backbone. We have also shown that Chro-metal complexes inhibit neuronal toxicity and suppresses locomotor deficits in a Drosophila model of C9orf72-associated ALS. The approach represents a new direction for drug discovery against ALS and FTD diseases by targeting G4C2 repeat motif DNA.


Asunto(s)
Esclerosis Amiotrófica Lateral/tratamiento farmacológico , Proteína C9orf72/genética , ADN de Forma A/ultraestructura , Demencia Frontotemporal/tratamiento farmacológico , Esclerosis Amiotrófica Lateral/genética , Esclerosis Amiotrófica Lateral/patología , Antracenos/química , Antracenos/farmacología , Complejos de Coordinación/química , Complejos de Coordinación/farmacología , ADN/efectos de los fármacos , ADN/ultraestructura , ADN de Forma A/efectos de los fármacos , Demencia Frontotemporal/genética , Demencia Frontotemporal/patología , Humanos , Conformación de Ácido Nucleico/efectos de los fármacos , Bibliotecas de Moléculas Pequeñas/farmacología
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