RESUMEN
The human microbiome contains genetic information that regulates metabolic processes in response to host health and disease. While acidic vaginal pH is maintained in normal conditions, the pH level increases in infectious vaginitis. We propose that this change in the vaginal environment triggers the biosynthesis of anti-vaginitis metabolites. Gene expression levels of Chryseobacterium gleum, a vaginal symbiotic bacterium, were found to be affected by pH changes. The distinctive difference in the metabolic profiles between two C. gleum cultures incubated under acidic and neutral pH conditions was suggested to be an anti-vaginitis molecule, which was identified as phenylacetic acid (PAA) by spectroscopic data analysis. The antimicrobial activity of PAA was evaluated in vitro, showing greater toxicity toward Gardnerella vaginalis and Candida albicans, two major vaginal pathogens, relative to commensal Lactobacillus spp. The activation of myeloperoxidase, prostaglandin E2, and nuclear factor-κB, and the expression of cyclooxygenase-2 were reduced by an intravaginal administration of PAA in the vaginitis mouse model. In addition, PAA displayed the downregulation of mast cell activation. Therefore, PAA was suggested to be a messenger molecule that mediates interactions between the human microbiome and vaginal health.
Asunto(s)
Chryseobacterium , Fenilacetatos , Vagina , Femenino , Animales , Fenilacetatos/metabolismo , Fenilacetatos/farmacología , Vagina/microbiología , Ratones , Humanos , Chryseobacterium/metabolismo , Candida albicans/metabolismo , Candida albicans/efectos de los fármacos , Simbiosis , Concentración de Iones de Hidrógeno , Gardnerella vaginalis/metabolismo , Gardnerella vaginalis/efectos de los fármacos , Modelos Animales de Enfermedad , Vaginitis/microbiología , Vaginitis/metabolismo , Vaginitis/tratamiento farmacológicoRESUMEN
El papanicolaou es un examen utilizado para la detección oportuna del cáncer cérvicouterino y a la vez ayuda a detectar los diferentes procesos inflamatorios e infecciosos del tracto genital inferior. El presente trabajo expone los resultados citológicos así como los gérmenes encontrados con mayor frecuencia en los frotis de un grupo de mujeres de la parroquia Atahualpa Ambato en el que el germen predominante fue la gardnerella vaginalis en un 29.87 por ciento del total de la muestra.