RESUMEN
Hypobiosis (facultative developmental arrest) is the most important life-cycle adaptation ensuring survival of parasitic nematodes under adverse conditions. Little is known about such survival mechanisms, although ascarosides (ascarylose with fatty acid-derived side chains) have been reported to mediate the formation of dauer larvae in the free-living nematode Caenorhabditis elegans. Here, we investigated the role of a key gene acox-1, in the larval development of Haemonchus contortus, one of the most important parasitic nematodes that employ hypobiosis as a routine survival mechanism. In this parasite, acox-1 encodes three proteins (ACOXs) that all show a fatty acid oxidation activity in vitro and in vivo, and interact with a peroxin PEX-5 in peroxisomes. In particular, a peroxisomal targeting signal type1 (PTS1) sequence is required for ACOX-1 to be recognised by PEX-5. Analyses on developmental transcription and tissue expression show that acox-1 is predominantly expressed in the intestine and hypodermis of H. contortus, particularly in the early larval stages in the environment and the arrested fourth larval stage within host animals. Knockdown of acox-1 and pex-5 in parasitic H. contortus shows that these genes play essential roles in the post-embryonic larval development and likely in the facultative arrest of this species. A comprehensive understanding of these genes and the associated ß-oxidation cycle of fatty acids should provide novel insights into the developmental regulation of parasitic nematodes, and into the discovery of novel interventions for species of socioeconomic importance.
Asunto(s)
Acil-CoA Oxidasa/metabolismo , Haemonchus/metabolismo , Proteínas del Helminto/metabolismo , Larva/metabolismo , Receptor de la Señal 1 de Direccionamiento al Peroxisoma/metabolismo , Animales , Hemoncosis/metabolismo , Haemonchus/crecimiento & desarrollo , Larva/crecimiento & desarrollo , Conejos , OvinosRESUMEN
Haemonchus contortus is a hematophagous endoparasite of small ruminants, which is responsible for huge economic losses in livestock sector. Hyaluronidase produced by infective larvae of H. contortus can degrade hyaluronic acid present in the host's abomasal tissue. Thus, it facilitates larval tissue invasion and early establishment. We herein explored this ability of hyaluronidase in H. contortus, and tested whether hyaluronidase is utilized as a virulence factor by H. contortus while establishing the infection. We first successfully blocked the hyaluronidase gene in L3 larvae by RNA interference (RNAi), which was subsequently confirmed by qPCR, enzymatic activity, and immunohistochemistry assays. Using these larvae we then conducted in vivo and in vitro assays on sheep to assess the effects of hyaluronidase suppression on larval invasion and establishment of infection. The in vivo assay showed a significant drop in worm burden in siRNA treated group in comparison to control group. During in vitro assay we applied an ovine ex vivo model where siRNA treated group of larvae showed significantly reduced invasion of the abomasal tissue explants as compared to control group. These findings indicate that hyaluronidase plays a key role in host's tissue invasion and larval establishment, and it is used as a virulence factor by H. contortus while establishing the infection. As an invasive virulence molecule, its functional research is thus conducive to the prevention of haemonchosis.
Asunto(s)
Hemoncosis/veterinaria , Haemonchus/fisiología , Proteínas del Helminto/metabolismo , Hialuronoglucosaminidasa/metabolismo , Enfermedades de las Ovejas/metabolismo , Animales , Hemoncosis/metabolismo , Hemoncosis/parasitología , Haemonchus/enzimología , Haemonchus/genética , Haemonchus/crecimiento & desarrollo , Larva/enzimología , Larva/genética , Larva/crecimiento & desarrollo , Larva/fisiología , Ovinos , Enfermedades de las Ovejas/parasitología , Oveja DomésticaRESUMEN
The aims of this study were to evaluate if the use of copper oxide wire particles, isolated or in association with closantel, in lambs infected with Haemonchus contortus enhances the anthelmintic efficacy of closantel, as well as to evaluate the effects of treatment in hepatic energy metabolism, inflammatory markers and hematological and biochemical tests. The lambs were randomly divided into five groups (6 animals each), as follows: uninfected animals (Control); animals infected with H. contortus (HC); infected and treated with closantel (HC + CL); infected and treated with copper oxide wire particles (HC + Cu); and infected and treated with closantel plus copper oxide wire particles (HC + CL + Cu). The animals of infected groups were infected orally with H. contortus (5,000 L3 -larvae) and on day 14 post infection (p.i) the treatments were initiated. The egg per gram of feces (EPG), butyrylcholinesterase (BuChE), myeloperoxidase (MPO), adenylate kinase (AK) and pyruvate kinase (PK) activities and hematological and biochemical tests were evaluated. Treatments with copper oxide (isolated and associated) were able to reduce the EPG count on days 28, 35, 42 and 49 p.i when compared to HC group, while closantel was able to reduce EPG only from day 35 p.i. Moreover, treatment with closantel (isolated or associated) was able to prevent the inhibition of hepatic AK and PK activities caused by H. contortus infection, which may contribute to efficient intracellular energetic communication in order to maintain the balance between cellular ATP consumption and production. Butyrylcholinesterase and MPO activities were higher in infected lambs compared to uninfected, while treated groups showed lower enzymatic activity compared to the group HC. The use of all therapeutic protocols was able to reduce the EPG count. Based on these evidences, the use of copper oxide plus closantel may be considered an alternative to treat lambs infected by H. contortus.
Asunto(s)
Antihelmínticos/administración & dosificación , Cobre/administración & dosificación , Hemoncosis/veterinaria , Inflamación/veterinaria , Salicilanilidas/administración & dosificación , Enfermedades de las Ovejas/tratamiento farmacológico , Abomaso/metabolismo , Adenilato Quinasa/metabolismo , Administración Oral , Animales , Antihelmínticos/farmacología , Antihelmínticos/uso terapéutico , Análisis Químico de la Sangre/veterinaria , Butirilcolinesterasa/sangre , Cápsulas , Metabolismo Energético/efectos de los fármacos , Recuento de Eritrocitos/veterinaria , Heces/parasitología , Hemoncosis/complicaciones , Hemoncosis/tratamiento farmacológico , Hemoncosis/metabolismo , Hematócrito/veterinaria , Hemoglobinas/análisis , Inflamación/tratamiento farmacológico , Inflamación/prevención & control , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/metabolismo , Masculino , Recuento de Huevos de Parásitos/veterinaria , Peroxidasa/sangre , Piruvato Quinasa/metabolismo , Distribución Aleatoria , Salicilanilidas/farmacología , Salicilanilidas/uso terapéutico , Ovinos , Enfermedades de las Ovejas/metabolismo , Enfermedades de las Ovejas/prevención & controlRESUMEN
The present study aimed to assess the antioxidant and oxidant status of goats naturally infected with Haemonchus contortus. Based upon the parasite burden, infection in goats was categorized as heavy (> 500 worms), mild (100-500 worms) or low (< 100 worms). Abomasal tissues from non-infected and infected goats were used for the determination of catalase (CAT), glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), aspartate (AST) and alanine (ALT) aminotransferases, acid (ACP) and alkaline (ALP) phosphatases, superoxide content (O2-), protein carbonyl (PC), malondialdehyde (MDA) and reduced glutathione (GSH). A significantly higher level of CAT, GST and GR activity and a lower level of GPx activity were recorded in infected compared to non-infected tissue. A significant increase in the level of AST, ALT, ALP and ACP was found in the abomasal tissue of the infected animals, which was related to the worm burden. The oxidative stress markers were also altered, with a significant decline in GSH levels, whereas MDA, PC and O2- concentrations showed a marked increase. In conclusion, it has been demonstrated that haemonchosis in goats resulted in considerable oxidative stress, which was directly related to the worm burden.
Asunto(s)
Antioxidantes/metabolismo , Enfermedades de las Cabras/metabolismo , Hemoncosis/veterinaria , Haemonchus/fisiología , Oxidantes/metabolismo , Animales , Catalasa/metabolismo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Glutatión Transferasa/metabolismo , Enfermedades de las Cabras/parasitología , Cabras , Hemoncosis/metabolismo , Hemoncosis/parasitología , Malondialdehído/metabolismo , Estrés Oxidativo , Superóxidos/metabolismoRESUMEN
The aim of this study was to evaluate the effect of copper edetate on biochemical parameters, oxidative profile, cholinesterase's activities, as well as its capacity to control gastrointestinal parasites in infected sheep. Thus, Lacaune sheep (n = 18) infected by Haemonchus contortus were used and divided into three groups of six animal each: the group A was composed of untreated animals (the control group), the group B was formed by animals treated with 0.3 mg/kg of copper edetate, and the group C was composed of animals treated with 0.5 mg/kg of copper edetate. Blood collection was performed on days 0, 10, 20 and 30 after mineral supplementation and different variables were measured. Cholinergic system was evaluated to determine the acetylcholinesterase (AChE) activity in total blood and butyrylcholinesterase (BChE) activity in serum. Eggs per gram of feces (EPG) were evaluated. There were no significant differences (P > 0.05) between groups regarding total protein, albumin, globulin and urea levels, GGT activity, as well as the hematocrit, and EPG. ALT activity decreased (P < 0.05) on groups B and C on day 30 compared to the control group (the group A). AChE activity decreased (P < 0.05) in the group C on days 10 and 30 compared to the control group, such decrease (P < 0.05) was also observed for BChE activity in the group C on day 10. ROS levels increased in the group C compared to groups A and B on day 10, while the SOD activity increased in the group C on days 20 and 30 compared to the control group (P < 0.05). CAT activity did not differ between groups (P > 0.05). In summary, the copper edetate was not efficient to control gastrointestinal parasites, but efficiently activated SOD, an important antioxidant enzyme. In addition, copper edetate was able to partially inhibit cholinesterase's activities when supplementated at its highest dose.
Asunto(s)
Colinesterasas/sangre , Ácido Edético/administración & dosificación , Hemoncosis/veterinaria , Haemonchus/efectos de los fármacos , Enfermedades de las Ovejas/parasitología , Superóxido Dismutasa/sangre , Acetilcolinesterasa/sangre , Animales , Butirilcolinesterasa/sangre , Inhibidores de la Colinesterasa/farmacología , Ácido Edético/farmacología , Activación Enzimática , Heces/parasitología , Hemoncosis/metabolismo , Hemoncosis/parasitología , Haemonchus/fisiología , Oviposición/efectos de los fármacos , Recuento de Huevos de Parásitos , Especies Reactivas de Oxígeno/metabolismo , Ovinos , Enfermedades de las Ovejas/metabolismoRESUMEN
BACKGROUND: The ghrelin axis is involved in the regulation of metabolism, energy balance, and the immune, cardiovascular and reproductive systems. The manipulation of this axis has potential for improving economically valuable traits in production animals, and polymorphisms in the ghrelin (GHRL) and ghrelin receptor (GHSR) genes have been associated with growth and carcass traits. Here we investigate the structure and expression of the ghrelin gene (GHRL) in sheep, Ovis aries. RESULTS: We identify two ghrelin mRNA isoforms, which we have designated Δex2 preproghrelin and Δex2,3 preproghrelin. Expression of Δex2,3 preproghrelin is likely to be restricted to ruminants, and would encode truncated ghrelin and a novel C-terminal peptide. Both Δex2 preproghrelin and canonical preproghrelin mRNA isoforms were expressed in a range of tissues. Expression of the Δex2,3 preproghrelin isoform, however, was restricted to white blood cells (WBC; where the wild-type preproghrelin isoform is not co-expressed), and gastrointestinal tissues. Expression of Δex2 preproghrelin and Δex2,3 preproghrelin mRNA was elevated in white blood cells in response to parasitic worm (helminth) infection in genetically susceptible sheep, but not in resistant sheep. CONCLUSIONS: The restricted expression of the novel preproghrelin variants and their distinct WBC expression pattern during parasite infection may indicate a novel link between the ghrelin axis and metabolic and immune function in ruminants.
Asunto(s)
Clonación Molecular , Ghrelina/metabolismo , Hemoncosis/veterinaria , Enfermedades de las Ovejas/metabolismo , Secuencia de Aminoácidos , Animales , Regulación de la Expresión Génica , Predisposición Genética a la Enfermedad , Ghrelina/genética , Hemoncosis/genética , Hemoncosis/metabolismo , Haemonchus , Leucocitos/metabolismo , Datos de Secuencia Molecular , Isoformas de Proteínas , Ovinos , Enfermedades de las Ovejas/genéticaRESUMEN
The aim of this study was to evaluate the acetylcholinesterase (AChE) activity in lymphocytes of lambs experimentally infected by Haemonchus contortus. A total of 14 healthy lambs were used, divided into two groups of seven animals each. Group A (negative control) represented the uninfected animals, and Group B (positive control) was formed by animals infected with 15,000 larvae of H. contortus. Blood was drawn on the days 15, 45 and 75 post-infection (PI) in order to perform the white blood cells (WBC) count, as well as the evaluation of AChE activity in lymphocytes. Parasitological stool exam (eggs per gram of feces - EPG) was performed on the same days to follow up the evolution of the infection. On day 15 PI it was verified negative EPG; however, on days 45 and 75 PI it was observed positive EPG only in the animals of group B. In the three evaluated periods was observed a lower number of leukocytes, associated with decreased lymphocytes and neutrophils in lambs infected by this gastrointestinal nematodes. Lambs infected with H. contortus showed significant (P<0.01) lower AChE activity in lymphocytes compared uninfected. Statistically, there was a positive correlation (P<0.05) between AChE activity in lymphocytes and number of lymphocytes (r=0.69). The lymphocytes are cells with direct participation in the cholinergic system; therefore, based on these results, it can be concluded that the experimental infection with H. contortus influences the number of lymphocytes, and consequently the AChE activity in these cells.
Asunto(s)
Acetilcolinesterasa/sangre , Hemoncosis/veterinaria , Linfocitos/enzimología , Enfermedades de las Ovejas/metabolismo , Animales , Heces/parasitología , Hemoncosis/sangre , Hemoncosis/metabolismo , Recuento de Leucocitos/veterinaria , Masculino , Recuento de Huevos de Parásitos/veterinaria , Ovinos , Enfermedades de las Ovejas/sangre , Enfermedades de las Ovejas/parasitologíaRESUMEN
The evolution of drug resistant bacteria is a severe public health problem, both in hospitals and in the community. Currently, some countries aim at concentrating highly specialized services in large hospitals in order to improve patient outcomes. Emergent resistant strains often originate in health care facilities, but it is unknown to what extent hospital size affects resistance evolution and the resulting spillover of hospital-associated pathogens to the community. We used two published datasets from the US and Ireland to investigate the effects of hospital size and controlled for several confounders such as antimicrobial usage, sampling frequency, mortality, disinfection and length of stay. The proportion of patients acquiring both sensitive and resistant infections in a hospital strongly correlated with hospital size. Moreover, we observe the same pattern for both the percentage of resistant infections and the increase of hospital-acquired infections over time. One interpretation of this pattern is that chance effects in small hospitals impede the spread of drug-resistance. To investigate to what extent the size distribution of hospitals can directly affect the prevalence of antibiotic resistance, we use a stochastic epidemiological model describing the spread of drug resistance in a hospital setting as well as the interaction between one or several hospitals and the community. We show that the level of drug resistance typically increases with population size: In small hospitals chance effects cause large fluctuations in pathogen population size or even extinctions, both of which impede the acquisition and spread of drug resistance. Finally, we show that indirect transmission via environmental reservoirs can reduce the effect of hospital size because the slow turnover in the environment can prevent extinction of resistant strains. This implies that reducing environmental transmission is especially important in small hospitals, because such a reduction not only reduces overall transmission but might also facilitate the extinction of resistant strains. Overall, our study shows that the distribution of hospital sizes is a crucial factor for the spread of drug resistance.
Asunto(s)
Ancylostoma/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/metabolismo , Depsipéptidos/farmacología , Haemonchus/metabolismo , Canales de Potasio de Gran Conductancia Activados por el Calcio/metabolismo , Actividad Motora/genética , Mutación , Ancylostoma/genética , Anquilostomiasis/tratamiento farmacológico , Anquilostomiasis/genética , Anquilostomiasis/metabolismo , Animales , Antihelmínticos/farmacología , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Depsipéptidos/antagonistas & inhibidores , Antagonismo de Drogas , Evaluación Preclínica de Medicamentos/métodos , Resistencia a Medicamentos/efectos de los fármacos , Resistencia a Medicamentos/genética , Regulación de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica/genética , Hemoncosis/tratamiento farmacológico , Hemoncosis/genética , Hemoncosis/metabolismo , Haemonchus/genética , Canales de Potasio de Gran Conductancia Activados por el Calcio/genética , Actividad Motora/efectos de los fármacos , Micotoxinas/farmacología , Especificidad de la EspecieRESUMEN
The aim of this study was to evaluate the in vitro immune modulation of two de novo peptides with hypothetical identity to the serine protease family (S28) from Haemonchus spp. Expression of mRNAs encoding these peptides was confirmed by RTqPCR in L3 and adult stage parasites. Antibodies from serum samples collected from an H. contortus-infected lamb at 60 days post infection detected both peptides, as assessed by indirect ELISA. Lamb peripheral blood mononuclear cells (PBMCs) were exposed to each peptide, as well as to the peptide mixture, and cell proliferation assays were performed at 24, 48 and 72 h. The relative expression of the IL4, IL5, IL6, IL13, CXCL8 and FCεR1A genes was quantified by RTqPCR from lamb PBMCs exposed to the peptide mixture at 24 and 48 h. With respect to immune gene expression, 15- and 3-fold upregulation at 24 h was observed with IL5 and CXCL8, respectively, and 2-fold upregulation of CXCL8 at 48 h. In contrast, downregulation of IL5 was stimulated at 48 h. These data suggest that these peptides (pep-hsp and pep-pcx), which show high identity with intestinal and excretion/secretion serine proteases, can trigger immunogenic activity, and suggest that they may be useful as potential parasite vaccines.
Asunto(s)
Hemoncosis , Haemonchus , Animales , Ovinos , Leucocitos Mononucleares/metabolismo , Serina Proteasas/genética , Serina Proteasas/metabolismo , Haemonchus/genética , Interleucina-5/genética , Interleucina-5/metabolismo , Regulación hacia Arriba , Hemoncosis/veterinaria , Hemoncosis/metabolismoRESUMEN
The influence of the administration route on the relationship between efficacy and ivermectin concentration profiles achieved in the bloodstream, the gastrointestinal mucosal tissues/fluid contents and within a target abomasal parasite (Haemonchus contortus) was evaluated in lambs. Twenty-six (26) parasitized lambs were assigned into three experimental groups: untreated (control) and ivermectin treated by the subcutaneous and intraruminal route at 0.2mg/kg. Blood samples were collected between 0 and 15 days post-treatment (plasma disposition study). Four animals from each group were sacrificed at day 3 post-treatment. Mucosa and content samples from abomasum and small intestine and adult specimens of H. contortus were collected. Drug concentrations were measured by HPLC. Individual fecal egg counts were evaluated at -1, 3 and 15 days post treatment. Post-mortem examination was done at day 15 post-treatment. Adult nematodes recovered from the digestive tract were counted and identified by species. Ivermectin plasma availability was higher (P<0.05) after the subcutaneous administration (129 ng.d/ml) compared to the intraruminal treatment (58.4 ng.d/ml). However, ivermectin concentrations measured in the gastrointestinal contents were higher in lambs treated by the intraruminal route. The mean ivermectin concentrations achieved (3 days post-treatment) in the abomasal content were 143 ng/g (intraruminal) and 2.53 ng/g (subcutaneous). Ivermectin concentrations were 15-fold higher in H. contortus recovered from intraruminally treated lambs. Whereas the subcutaneous administration reduced the number of adult nematodes from 4376 to 1300, the number of adult nematodes after the treatment with ivermectin given by the intraruminal route was 206 (P<0.05). The higher ivermectin concentrations achieved in the digestive tract shortly after the intraruminal treatment may account for the observed enhanced efficacy compared to the parenteral administration against parasites of reduced susceptibility.
Asunto(s)
Antiparasitarios/administración & dosificación , Hemoncosis/veterinaria , Haemonchus/efectos de los fármacos , Ivermectina/administración & dosificación , Enfermedades de las Ovejas/tratamiento farmacológico , Abomaso/metabolismo , Abomaso/parasitología , Administración Oral , Animales , Antiparasitarios/análisis , Antiparasitarios/farmacocinética , Antiparasitarios/farmacología , Área Bajo la Curva , Resistencia a la Enfermedad , Heces/parasitología , Tracto Gastrointestinal/metabolismo , Tracto Gastrointestinal/parasitología , Hemoncosis/tratamiento farmacológico , Hemoncosis/metabolismo , Haemonchus/metabolismo , Inyecciones Subcutáneas/veterinaria , Ivermectina/análisis , Ivermectina/farmacocinética , Ivermectina/farmacología , Yeyuno/metabolismo , Yeyuno/parasitología , Recuento de Huevos de Parásitos/veterinaria , Rumen , Ovinos , Enfermedades de las Ovejas/metabolismo , Enfermedades de las Ovejas/parasitologíaRESUMEN
The aim of this project was to study the influence of haemonchosis, a common parasitic infection of small ruminants caused by Haemonchus contortus, on the activity of biotransformation enzymes and on in vitro flubendazole (FLU) biotransformation in liver and small intestine of lambs (Ovis aries). Twelve lambs were divided into three groups: non-infected animals, animals orally infected with larvae of H. contortus ISE strain for 7 weeks and for 11 weeks. At the end of the experiment, hepatic and intestinal subcellular fractions were prepared and used for assays of biotransformation enzymes activities and FLU metabolism testing. The activities of hepatic cytochromes P450, flavine monooxygenases and carbonyl-reducing enzymes were decreased in infected animals. UDP-glucuronosyl transferase activity was significantly lower (by 35%) in 11 weeks infected animals than that in control animals. When in vitro metabolism of FLU was compared in control and infected animals, significantly lower velocity of FLU reduction was found in infected animals. Slower FLU reduction may be beneficial for the haemonchosis treatment using FLU, because FLU will remain longer in the organism and could cause longer contact of parasites with FLU.
Asunto(s)
Hemoncosis/veterinaria , Mebendazol/análogos & derivados , Enfermedades de las Ovejas/metabolismo , Animales , Biotransformación , Hemoncosis/tratamiento farmacológico , Hemoncosis/metabolismo , Haemonchus/efectos de los fármacos , Hígado/enzimología , Hígado/metabolismo , Masculino , Mebendazol/metabolismo , Mebendazol/uso terapéutico , OvinosRESUMEN
The aim of this study was to evaluate the effects of medicinal herbal mixtures rich in phenolic, flavonoid and alkaloid compounds on ruminal fermentation and microbial populations, and fatty acid (FA) concentrations and lipid oxidation in tissues of lambs infected with the gastrointestinal nematode (GIN) parasite (Haemonchus contortus). Parallel in vitro and in vivo studies were performed using two different herbal mixtures (Mix1 and Mix2). The in vitro study was conducted in a 2 (infection status; non-infected versus infected) × 3 (diets; control, Mix1 and Mix2) factorial design. In the in vivo study, 24 lambs were equally divided into four treatments: non-infected lambs fed a control diet, infected lambs fed the control diet, infected lambs fed a diet with Mix1 and infected lambs fed a diet with Mix2. Herbal mixtures (100 g dry matter (DM)/d) were added to the basal diets of meadow hay (ad libitum) and a commercial concentrate (500 g DM/d). The experimental period lasted for 70 days. Ruminal fermentation characteristics and methane production were not affected by infection in vivo or in vitro. Both herbal mixture supplementation increased total volatile fatty acid (VFA) concentrations (P < 0.01) and DM digestibility (P < 0.01) in vitro. Archaea population was slightly diminished by both herbal mixtures (P < 0.05), but they did not lower methane production in vitro or in vivo (P > 0.05). Infection of H. contortus or herbal mixtures modulated FA proportion mainly in the liver, especially the long chain FA proportion. Concentrations of thiobarbituric acid reactive substances (TBARS) in serum were significantly higher after 70 days post-infection in the infected lambs. Herbal Mix1 supplementation reduced TBARS concentrations in meat after seven days of storage. In conclusion, supplementing of herbal mixtures to the diets of GIN parasite infected lambs did not affect the basic ruminal fermentation parameters. Herbal mixtures may improve few FA proportions mainly in liver as well as decrease lipid oxidation in meat.
Asunto(s)
Suplementos Dietéticos , Fermentación/efectos de los fármacos , Microbioma Gastrointestinal/efectos de los fármacos , Hemoncosis/veterinaria , Haemonchus , Metabolismo de los Lípidos/efectos de los fármacos , Plantas Medicinales/metabolismo , Rumen/metabolismo , Enfermedades de las Ovejas/parasitología , Animales , Dieta/veterinaria , Ácidos Grasos/metabolismo , Femenino , Hemoncosis/metabolismo , Rumen/microbiología , Rumen/parasitología , Ovinos/metabolismo , Ovinos/microbiología , Ovinos/parasitología , Enfermedades de las Ovejas/metabolismoRESUMEN
Sheep have a varying ability to resist infection with gastrointestinal nematodes. This ability is due in part to genetic differences that exist between individuals. In order to define these differences we have used real-time PCR to quantify gene expression responses in the gut mucosal surface of genetically resistant and susceptible sheep, following a nematode challenge. Expression profiles were determined in response to two different nematode species, Haemonchus contortus and Trichostrongylus colubriformis, and in divergent sheep originating from two different genetic backgrounds. Results show that the response generated differs between resistant and susceptible animals and is further impacted by the origin of the sheep and nematode species used for challenge. However, some conserved features of a response mounted by a resistant or a susceptible animal were identified. Genes found to be more abundantly expressed in resistant animals include markers of an early inflammatory response, several Toll-like receptors (TLR2, 4, 9) and free radical producing genes (DUOX1 and NOS2A). Conversely, genes differentiating susceptible animals indicate a prolonged response and development of a chronic inflammatory state, characterised by elevated expression of members of the NF-kappabeta signalling pathway (IKBKB and NFKBIA) together with delayed expression of regulatory markers such as IL2RA (CD25), IL10 and TGFbeta2. While multiple nematode response pathways were identified, the identification of conserved aspects of the response which associate with resistance provides evidence that alternative nematode control strategies, such as breeding for resistant animals, may be feasible.
Asunto(s)
Secuencia Conservada , Hemoncosis/metabolismo , Parasitosis Intestinales/metabolismo , Mucosa Intestinal/metabolismo , Enfermedades de las Ovejas/metabolismo , Tricostrongiliasis/metabolismo , Animales , Expresión Génica , Predisposición Genética a la Enfermedad , Haemonchus , Mucosa Intestinal/parasitología , Recuento de Huevos de Parásitos , Parasitología/métodos , Ovinos , Enfermedades de las Ovejas/parasitología , TrichostrongylusRESUMEN
Calreticulin (CalR), a Ca(2+) binding multifunctional protein, is secreted by the parasitic nematode Haemonchus contortus. We have earlier observed binding of this protein to a 24-kDa polypeptide (p24) present in an enriched preparation of prothrombin. In the present study, the identity of p24 was established as a C-reactive protein (CRP) by several criteria. CalR binding to CRP is an elegant strategy devised by the parasite to survive in the host. The secreted CalR may achieve this either by limiting the free concentration of CRP, which has antiparasite activity or inhibit the activation of the classical complement pathway triggered on binding of CRP to C1q protein. CalR binding to CRP would also ensure a check on the procoagulant activity of the CRP enabling parasite to feed on the host blood. Thus, targeting CalR could be a novel strategy to tackle this parasite, which has developed resistance to many anthelmintics.
Asunto(s)
Proteína C-Reactiva/metabolismo , Calreticulina/metabolismo , Hemoncosis/inmunología , Hemoncosis/metabolismo , Haemonchus/fisiología , Proteínas del Helminto/metabolismo , Secuencia de Aminoácidos , Animales , Proteína C-Reactiva/genética , Proteína C-Reactiva/aislamiento & purificación , Calreticulina/inmunología , Activación de Complemento , Complemento C1q/metabolismo , Cabras/sangre , Cabras/inmunología , Datos de Secuencia MolecularRESUMEN
Previously, chemical analysis of gastric fundic mucin showed that infection of sheep with Haemonchus contortus or Teladorsagia circumcincta changed the proportions of monosaccharides and decreased terminal mucin fucosylation and sialylation. To identify the effects of these parasites on the two mucin-secreting cell lineages, fundic and antral tissues were collected for histochemistry from 69 lambs aged from 3-4 to 9-10 months-of-age which had received a single infection of either H. contortus or T. circumcincta and euthanased at Day 21 or 28 post- infection respectively. All fundic tissues were stained separately with: (1) with Periodic Acid Schiff (PAS) for all mucins; (2) Alcian Blue (AB) pH 2.5 for acidic mucins (sialylated and sulphated); (3) AB pH 1 for sulphated mucins and (4) High Iron Diamine (HID) for sulphated mucins. Antral and fundic tissues from 24 lambs were also stained for acidic and neutral mucins or with specific lectins for α-1-linked fucose and for α-2,3- and α-2,6-linked sialic acids. Only mucin sulphation appeared to differ visually in uninfected lambs over this age range: there was weak staining with HID in tissues from lambs 3-6 months-of-age, but was generally more intense in those over 7 months-of-age. Sulphomucins were not apparent in surface mucous cells (SMC) or generally in the upper pits. Sialylomucins were located predominantly in the pits and glands, with small amounts of sialylated mucins in SMC and on the luminal surface, mainly in younger animals up to 6 months-of-age and less in the older animals. Parasitism markedly reduced the predominantly neutral surface mucin5AC of the SMC and pit cells, despite pit elongation in both antrum and fundus, whereas the acidic Muc6 secreted by mucus neck cells (MNC) increased along with MNC hyperplasia. Sulphated mucins were present mainly from the mid-pits downward and heavy staining was more common in older animals. In these sheep, the markedly reduced neutral mucin in the SMC and pit cells in both antrum and fundus contrasts with reported hypersecretion of mucus in the intestine, which is believed to aid in parasite expulsion. It has been proposed that intestinal goblet cell hypersecretion occurs only in resistant animals, therefore reduced mucins in the abomasum may be indicative of susceptibility to abomasal parasites.
Asunto(s)
Abomaso/metabolismo , Haemonchus/metabolismo , Mucinas/metabolismo , Enfermedades de las Ovejas/metabolismo , Trichostrongyloidea/metabolismo , Tricostrongiloidiasis/veterinaria , Abomaso/parasitología , Abomaso/patología , Factores de Edad , Animales , Heces/parasitología , Fundus Gástrico/metabolismo , Fundus Gástrico/parasitología , Fundus Gástrico/patología , Glicosilación , Hemoncosis/metabolismo , Hemoncosis/veterinaria , Lectinas/metabolismo , Masculino , Mucina 5AC/metabolismo , Mucina 6/metabolismo , Mucinas/clasificación , Naftoquinonas , Antro Pilórico/metabolismo , Antro Pilórico/parasitología , Antro Pilórico/patología , Ovinos , Enfermedades de las Ovejas/parasitología , Tricostrongiloidiasis/metabolismoRESUMEN
The effect of Haemonchus contortus infection in sheep fed with a moderate and high protein content diet was evaluated in two breeds of sheep. Forty-eight Ile de France and Santa Ines lambs were maintained indoors since birth, in worm-free conditions. The lambs were allocated after weaning in four groups of six animals per breed, which were either infected or remain uninfected and given access to either a moderately or highly metabolizable protein diet. The moderately and highly metabolizable protein diets were calculated to supply 75 and 129 g metabolizable protein per kg of dry matter (MP/kg DM), respectively. The infection consisted of a trickle infection with 300 infective larvae, three times a week, for 12 weeks. Significant differences were observed for mast cell, globule leukocyte and eosinophil counts in the abomasal mucosa of the infected groups compared to the control of both breeds (P<0.05), regardless of the diet supplied. Significantly higher IgA anti-L5 antibody was detected in the infected Santa Ines groups than in the infected Ile de France groups (P<0.05). Increased metabolizable protein supply resulted in larger body weight gain and higher packed cell volumes for both breeds (P<0.05). Both breeds showed an increased ability to withstand the pathophysiological effects of H. contortus infection when given access to the highly metabolizable protein diet. However, increased metabolizable protein supply resulted in reduced worm burdens in Santa Ines lambs but not in the Ile de France lambs (P<0.05). The present results show that the increase in protein content in growing lamb diets may benefit resistance and resilience to gastrointestinal parasites but that these benefits may vary among breeds.
Asunto(s)
Proteínas en la Dieta/administración & dosificación , Enfermedades Gastrointestinales/veterinaria , Hemoncosis/veterinaria , Haemonchus/crecimiento & desarrollo , Enfermedades de las Ovejas/parasitología , Abomaso/parasitología , Animales , Recuento de Células Sanguíneas/veterinaria , Peso Corporal/fisiología , Proteínas en la Dieta/inmunología , Proteínas en la Dieta/metabolismo , Ensayo de Inmunoadsorción Enzimática/veterinaria , Heces/parasitología , Femenino , Mucosa Gástrica/parasitología , Enfermedades Gastrointestinales/inmunología , Enfermedades Gastrointestinales/metabolismo , Enfermedades Gastrointestinales/parasitología , Predisposición Genética a la Enfermedad , Hemoncosis/metabolismo , Hemoncosis/parasitología , Hematócrito/veterinaria , Histamina/análisis , Histocitoquímica/veterinaria , Modelos Lineales , Masculino , Ovinos , Enfermedades de las Ovejas/inmunología , Enfermedades de las Ovejas/metabolismoRESUMEN
Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.
Asunto(s)
Colectinas/biosíntesis , Galectinas/biosíntesis , Hemoncosis/veterinaria , Enfermedades de las Ovejas/metabolismo , Animales , Colectinas/genética , Femenino , Galectinas/genética , Expresión Génica , Hemoncosis/genética , Hemoncosis/metabolismo , Haemonchus , Masculino , OvinosRESUMEN
Ujumqin sheep are susceptible to infection by the gastrointestinal nematode Haemonchus contortus, which reduces productivity and total meat yield in sheep. Thus, the effects of green tea polyphenol (GTP) supplements (0, 2, 4, or 6g of GTP/kg feed) on dietary nutrient digestibility and meat quality in lambs infected with H. contortus were examined; control lambs were not infected. H. contortus infections did not affect digestion but the apparent digestibilities of nutrients were decreased by dietary 2g of GTP/kg feed supplementation. There was an interaction between treatment and sampling time on plasma total protein, urea nitrogen, and amino acid concentrations. The antioxidant activity and meat color of INFGTP0 lambs decreased. In conclusion, H. contortus infections in lambs decreased meat quality, but appropriate levels of dietary GTP supplementation diminished these negative effects though lower dose of GTP supplement showed negative effects on digestion.
Asunto(s)
Dieta/veterinaria , Digestión , Hemoncosis/veterinaria , Músculo Esquelético/metabolismo , Polifenoles/uso terapéutico , Enfermedades de las Ovejas/dietoterapia , Té/química , Aminoácidos/análisis , Aminoácidos/sangre , Animales , Antioxidantes/administración & dosificación , Antioxidantes/efectos adversos , Antioxidantes/análisis , Antioxidantes/uso terapéutico , Camellia sinensis/química , China , Dieta/efectos adversos , Heces/química , Heces/parasitología , Manipulación de Alimentos , Calidad de los Alimentos , Hemoncosis/dietoterapia , Hemoncosis/metabolismo , Hemoncosis/parasitología , Haemonchus/aislamiento & purificación , Masculino , Carne/análisis , Carne/parasitología , Músculo Esquelético/química , Pigmentos Biológicos/análisis , Pigmentos Biológicos/metabolismo , Hojas de la Planta/química , Polifenoles/administración & dosificación , Polifenoles/efectos adversos , Ovinos , Enfermedades de las Ovejas/sangre , Enfermedades de las Ovejas/metabolismo , Enfermedades de las Ovejas/parasitología , Oveja Doméstica , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
Abomasal pH and serum pepsinogen and gastrin were increased in parasite-naive sheep by infection with either larval or adult H. contortus. Four sheep received 10000 larvae intraruminally and 9000 adult worms were given directly via an abomasal cannula to another 4 sheep. The latter animals were dosed orally with 0.4 mg kg-1 ivermectin 4 days after worm transfer and their recovery was monitored for a further 8 days. Whereas the presence of adult worms rapidly induced changes in the secretory activity of the abomasum, the early larval stages had minimal effects up to the 4th day post-infection. After either larval or adult infection, the initial hypergastrinaemia began at the same time as the increase in abomasal pH, but serum gastrin continued to increase after abomasal pH had reached a maximum and returned more slowly to normal values after drenching. The increase in serum pepsinogen did not precede those in the other parameters, unlike the earlier hyperpepsinogenaemia which occurs with Ostertagia infection. Three of the 8 infected sheep had no detectable serum pepsinogen increase during the parasitism while showing typical effects on abomasal pH and serum gastrin. The rapidity of the responses to the transfer of adult worms and to their removal by treatment with anthelmintic supports a role for worm excretory/secretory (ES) products which possibly are not produced by the early larval stages. The similarity of responses to H. contortus and O. circumcincta infection suggests the involvement of the same or very similar ES products.
Asunto(s)
Abomaso/metabolismo , Gastrinas/sangre , Hemoncosis/veterinaria , Haemonchus/crecimiento & desarrollo , Pepsinógenos/sangre , Enfermedades de las Ovejas/metabolismo , Animales , Heces/parasitología , Hemoncosis/metabolismo , Hemoncosis/parasitología , Concentración de Iones de Hidrógeno , Larva/crecimiento & desarrollo , Recuento de Huevos de Parásitos , Ovinos , Enfermedades de las Ovejas/parasitologíaRESUMEN
Within 12 h of reducing the daily intake of a 50:50 lucerne and wheaten hay ration from 800 to 400 g, there was an increase in digesta marker concentration indicating a reduction in the flow rate of digesta fluid and particulates through the abomasum of sheep. The efficacy of the recommended dose of oxfendazole (OFZ) was then tested against benzimidazole resistant Trichostrongylus colubriformis and Haemonchus contortus in sheep whose feed intake was halved 36 h before and after drug treatment. In animals fed the reduced ration there was a 60% reduction of T. colubriformis and 94% reduction of H. contortus compared with a 19% and 60% reduction respectively on the higher feed intake. It is therefore suggested that greater anthelmintic efficacy can be achieved in sheep by temporary feed reduction.