Leukocyte migration in ovarian carcinoma: comparison of inhibitory activity of tumor extracts.

Inhibition of migration of leukocytes from patients with serous cystadenocarcinoma of the ovary was studied by the use of several different types of ovarian carcinoma extract as antigen. KCl extract of an ovarian carconoma was found to be the most effective antigen preparation in comparison with saline, deoxycholate, and perchloric acid extracts. Low concentrations of KCl ovarian carcinoma extract significantly inhibited migration of leukocytes from 11 of 17 patients with ovarian carcinoma (migration index, less than 0.74). Leukocytes from patients with breast, colon, or endometrial carcinoma showed minimal reactivity with ovarian carcinoma KCl extract, and leukocytes from patients with ovarian carcinoma showed minimal reactivity with KCl extracts of breast, colon, and endometrial carcinoma. These results suggested that the 3 M KCl procedure is superior for the isolation of antigens active in the leukocyte migration inhibition test and that this test may be of use for the isolation of tumor-associated antigen and the immunodiagnosis of ovarian carcinoma.

Brief communication: technical modifications of the human agarose microdroplet leukocyte migration inhibition assay.

Direct leukocyte migration inhibition assays using the capillary tube technique can be used to demonstrate cell-mediated immunity in vitro. Unfortunately, the cumbersome nature of this technique makes it time consuming and difficult to perform. Similar results have been obtained using the direct agarose microdroplet leukocyte migration inhibition assay. In this paper, modifications of the agarose technique are outlined which insure standardization of droplets and ease of performance of the assay. Additionally a technique is described to reduce the time required for calculation of results.

The macrophage electrophoretic mobility test (MEM) some technical considerations.

The following parameters of the cytopherometric test system have been studied. 1. Temperature is less important for the production of macrophage slowing factor (MSF) by lymphocyte-antigen interaction than it is for the activity of MSF on macrophages. 2. Lymphocyte number titration curves allow discrimination between 'weak' and 'strong' antigen responses. 3. Lymphocyte-antigen reaction reaches a plateau after 30 min incubation, whilst maximum MSF-macrophage reaction requires 60 min. 4. Interaction of MSF with macrophages is sensitive to cycloheximide. 5. Irradiation of peritoneal exudate cells (200 rad) is essential in order to obtain a maximal result in the MEM test. In the 'split MEM test, 200 rad to the indicator peritoneal exudate cells produces a greater result, i.e. it in some way amplifies the macrophage response to MSF. 6. Excess of peritoneal exudate cells, especially when non-irradiated, suppresses the test result. This is not due to consumption of MSF produced. Probably macrophages rather than lymphocytes are responsible for the suppression.

Leukocyte migration inhibitory factor in HSV infections.

The cell-mediated immune (CMI) response as measured by a direct assay of leukocyte migration inhibition factor (LMIF) was determined in a population of patients with recurrent herpes simplex virus (HSV) infections in the quiescent stage as well as in healthy volunteers. The migration of leukocytes incubated in the presence of HSV antigens was compared to that without viral antigens for the calculation of the migration index (MI). Eleven of 41 control subjects (16.8%) had a MI below 0.8, indicating a positive CMI response. In contrast, all the herpes patients tested had a MI above 0.8, suggesting an impairment in the production of LMIF at this stage of their disease. This difference was statistically significant (t = 4.296; p less than 0.001) and was not dependent on the age of the population. This study indicates that individuals with recurrent HSV infections have impaired CMI response betweeen attacks which may be associated with the stage of the disease.

[Macrophage electrophoretic mobility inhibition test (MET) in immunological diagnosis of malignant lymphomas in Papio baboons]. / Metod tormozheniia elektroforeticheskoi podvizhnosti makrofagov (MEM-test) v immunodiagnostike zlokachrstvennykh limfom pavianov gamadrilov.

Reactivity of lymphocytes from P. hamadryas monkeys carrying malignant lymphomas, directed against soluble antigens from allogeneic lymphomas, was investigated, using macrophage electrophoretic mobility (MEM) test. Twenty-three monkeys have been examined. Fourteen of them carried malignant lymphoma, including those with initial manifestations of malignant lymphoma, animals with a generalized process and those in the period of spontaneous remission, and 9 control animals (8 healthy baboons and one animal wih hepatoma). The macrophage electrophoretic mobility reduction higher than 10% was considered as positive. A positive reaction was observed in 78% of monkeys with malignant lymphoma. The positive MEM-test was noted already in the initial stage of the disease, and it remained positive in the period of spontaneous remission.

[Migration of polynuclear neutrophils in vivo. Standardization of the technic]. / Contribution à l'étude de la migration des polynucléaires neutrophiles in vivo. Standardisation de la technique.

The migration of neutrophils across a skin barrier is a commonly employed method for the study of the inflammatory response. This report aims at comparing the leukocyte migration in the presence of two different chemoattractants : autologous serum and pool of serum. The reproducibility of this method has proved to be increasing by using duplicate skin chambers in 30 healty volunteers. The neutrophil migration with the pool of serum appears more homogeneous than with autologous serum. These results permit to obtain a standard curve of the neutrophil migration using a pool of serum.

[Examination of cell-mediated immunity using the leukocyte migration inhibition test in agarose]. / Prüfung der zellvermittelten Immunität mittles Leukozytenmigrationshemmtestes in Agarose

The 2-phase-leucocyte-migration-inhibition-test in agarose after Clausen is suitable for the proof of the cell-mediated immunity. Compared with the capillary technique of Soborg and Bendixen it has some methodical advantages. Between the migration inhibition against tuberculin and the result of the intracutaneous tuberculin testing a significant negative correlation was the result. The migration inhibition against a streptolysin-streptokinase-mixture was provable in 21 out of 22 patients with renal insufficiency in the same size as in clinically healthy persons.

Leukocyte migration inhibition in bacillus Calmette-Guérin vaccinated healthy adults and in tuberculosis assayed by a rapid photoelectric procedure.

A sensitive photoelectric method was used for reading migrations of human peripheral blood leukocytes from agarose microdroplets. Its rapidity enabled the use of a wide range (12 logs) of in vitro concentrations of antigen. Inhibition of migration of leukocytes (LMI) of healthy subjects having had BCG vaccination in childhood was found to occur in two zones of PPD concentrations, one high, from 10(-1) to 1000 micrograms/ml with a peak at 100 micrograms/ml, and one low, from 10(-8) to 10(-2) micrograms/ml. While three-fifths of subjects showed high zone LMI, in one-fifth it was bizonal and in another fifth observed only in the low zone. In patients with active pulmonary tuberculosis LMI, prior to all treatment, was reduced, absent, or replaced by enhanced migration, particularly in the low zone or in both zones. One and a half to four months after treatment LMI was found to be bizonal, enhanced migration having disappeared. These observations suggest the participation of two cell populations with widely different sensitivity to PPD in LMI in tuberculous and BCG vaccinated subjects and the presence of migration stimulatory lymphokine(s) during active tuberculosis.