RESUMO
BACKGROUND: Phytochemical compounds can modify the rumen microbiome and improve rumen fermentation. This study evaluated the impact of supplementation with tannin and an herbal mixture containing ginger (Zingiber officinale), garlic (Allium sativum), Artemisia (Artemisia vulgaris), and turmeric (Curcuma longa) on the rumen fermentation and microbiota, and histology of rumen tissue of goats. Eighteen Shami male goats were divided into three groups (n = 6): non-supplemented animals fed the basal diet (C, control); animals fed basal diet and supplemented with condensed tannin (T); and animals fed basal diet and supplemented with herbal mixture (HM). Each animal received a basal diet composed of Alfalfa hay and a concentrate feed mixture. RESULTS: Group HM revealed higher (P < 0.05) rumen pH, total volatile fatty acids (VFA), acetic, propionic, isobutyric, butyric, isovaleric, and valeric. Principal Co-ordinate analysis (PCoA) showed that rumen microbial communities in the control group and supplemented groups were distinct. The supplementation increased (P < 0.05) the relative abundances of phylum Bacteroidota and Proteobacteria and declined (P < 0.05) Firmicutes and Fibrobacterota. Additionally, the dominant genus Prevotella and Rikenellaceae RC9 gut group were increased (P < 0.05) and the family Ruminococcaceae was declined (P < 0.05) due to the supplementation. The supplementation decreased (P < 0.05) the archaeal genus Methanobrevibacter and increased (P < 0.05) Candidatus Methanomethylophilus. Tannin supplementation in T group shortened the rumen papillae. CONCLUSIONS: The results revealed that the herbal mixture might be used to alter the rumen microbiota to improve rumen fermentation.
Assuntos
Alho , Microbiota , Proantocianidinas , Masculino , Animais , Proantocianidinas/metabolismo , Taninos/farmacologia , Rúmen/metabolismo , Fermentação , Cabras , Dieta/veterinária , Ração Animal/análiseRESUMO
OBJECTIVE: To evaluate histologically and radiographically the potential of dog's immature roots with apical periodontitis to regenerate after regenerative endodontic treatment using mesoporous silica nanoparticles (MSNs) with/without bone morphogenic protein (BMP-2) as scaffolds. METHODS: In 4 mongrel dogs, 56 immature teeth with 96 roots were infected, resulting in necrotic pulps and periapical pathosis. According to the evaluation time (Group I = 30 days and Group II = 90 days), 90 roots were divided into two equal groups (45 roots each) and 6 roots used to replace any lost root during the procedure. The two main groups were further divided according to treatment protocol into 5 subgroups (9 roots each): blood clot (BC subgroup), mesoporous silica nanoparticles scaffold only (MSNs subgroup), mesoporous silica nanoparticles impregnated with BMP2 (MSNs + BMP2 subgroup), infected teeth without treatment (+ ve control subgroup) and normal untouched teeth (-ve control subgroup). All teeth surfaces were coated with Tincture iodine and calcium hydroxide was applied prior to treatment protocols. Then, teeth were restored with glass ionomer filling to seal the remaining part of the access cavity. Radiography evaluation of the increase in root length, root thickness and occurrence of apical closure were performed. Following the sacrifice of the two dogs at each time of evaluation, histopathological analysis was performed and included the inflammatory cells count, bone resorption, tissue ingrowth, deposition of hard tissue, and closure of the apical part. All data were statistically analyzed. RESULTS: Compared to BC subgroup, MSNs and MSNs + BMP-2 subgroups exhibited significant higher increase in root length and thickness as well as higher vital tissue in-growth and new hard tissue formation in group II (P < 0.05). MSNs + BMP-2 subgroup had significant higher increase in root length and thickness as well as significant lower inflammatory cell count than MSNs subgroup in both groups (P < 0.05). There were no significant differences between MSNs and MSNs + BMP-2 subgroups regarding new hard tissue formation in both groups and apical closure in group I (P > 0.05). CONCLUSION: MSNs with/without BMP-2 scaffolds enabled the continuing growth of roots in immature teeth with necrotic pulps and periapical pathosis. Addition of BMP-2 to MSNs scaffold improved its outcome in regenerative endodontics. CLINICAL RELEVANCE: MSNs with/without BMP-2 scaffolds may alternate blood clot for regenerative endodontic treatment of immature teeth with necrotic pulps.
Assuntos
Polpa Dentária , Nanopartículas , Dióxido de Silício , Alicerces Teciduais , Raiz Dentária , Animais , Cães , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/diagnóstico por imagem , Proteína Morfogenética Óssea 2 , Periodontite Periapical/terapia , Periodontite Periapical/patologia , Necrose da Polpa Dentária/terapia , Regeneração/efeitos dos fármacos , Endodontia Regenerativa/métodosRESUMO
The present study evaluated the effects of forage sources on dry matter (DM) intake, digestibility, and fermentation parameters in camels vs. sheep. The study was arranged as a 2 × 3 factorial experiment in a completely randomized design by using two animal species (three ruminally cannulated female camels and three male sheep) and three forage sources. The forages were (1) alfalfa hay; 164 and 479 g/kg DM for crude protein (CP) and neutral detergent fiber (NDF), respectively, (2) berseem hay; 121 and 513 g/kg DM for CP and NDF, respectively, and (3) wheat straw (27.5 and 723 g/kg DM for CP and NDF, respectively). Higher DM intake [g/kg body weight (BW)] was noted in sheep compared to camel when alfalfa hay (p < 0.05) and berseem hay (p < 0.05) were fed but was similar between both species when they were fed wheat straw. Forage type rather than animal species had more effect on metabolic intake. Lower digestibility was noted in sheep with wheat straw (p < 0.05). Similar in situ degradability values for crude protein and DM were noted between camels and sheep. In situ degradability of NDF was higher (p < 0.05) in camel than sheep. Greater ruminal pH (p < 0.05) was noted in sheep vs. camels when berseem hay and wheat straw were fed. Lower ruminal passage rate (p < 0.05) was noted in camels and higher total mean retention time. It was concluded that sheep and camels had similar digestion capacities when fed berseem hay and alfalfa hay, however, camels are more efficient than sheep when fed wheat straw.
Assuntos
Camelus , Digestão , Animais , Feminino , Masculino , Ração Animal/análise , Dieta/veterinária , Fermentação , Rúmen/metabolismo , OvinosRESUMO
Direct fed microbial may enhance the utilization of halophyte forages leading to improved animal growth and productivity. This study was conducted to evaluate Atriplex hay-based diet supplemented with yeast (Saccharomyces cerevisiae; SC) or bacteria (Bacillus subtilis and Lactobacillus casei; BAC) on lamb growth performance, digestibility, rumen fermentation, and carcass characteristics. Fifteen Barki lambs (90 ± 7 days of age and 18.6 ± 0.41 kg SE body weight; BW) were randomly assigned to three treatments for 120 days as follows: Control (basal diet without supplementation), SC and BAC diets, the basal diet supplemented with SC or BAC at 2 g/head/day, respectively. All lambs had similar dry matter (DM) intake, while lambs fed SC or BAC dies had higher (P < 0.05) total gain and average daily gain than those fed the control diet. Supplementation of SC or BC increased (P < 0.05) the digestibility of DM, organic matter, and acid detergent fiber, tended to decrease (P < 0.05) the urine N excretion and enhanced the N balance compared to the control. Ruminal pH, acetate, total volatile fatty acids concentrations, and bacterial protein were increased (P < 0.05), while creatinine and urea concentrations were decreased (P < 0.05) by both additives. Compared to other diets, the BAC diet reduced (P < 0.05) triglycerides, total lipids, kidney fat, and eye muscle fat. In conclusion, both additives resulted in similar positive growth performance and feed utilization, while only the BAC additive had a beneficial advantage in reducing the fat content of the carcass.
Assuntos
Atriplex , Saccharomyces cerevisiae , Animais , Ovinos , Rúmen/metabolismo , Fermentação , Ração Animal/análise , Digestão/fisiologia , Suplementos Nutricionais , Dieta/veterinária , Nutrientes , Carneiro Doméstico , BactériasRESUMO
The present work aims to study gamma rays emitted by radionuclides such as 238U, 232Th and 40K from acidic Monqul volcanics. The studied volcanics are represented by a thick stratified lava flows interbanded with their pyroclastics. They are composed of thick lava flows of andesite and, to a lesser extent of basalt, and acidic volcanics including rhyolite and dacite. The average values of 238U, 232Th and 40K are (46 ± 24 Bq kg-1), (62 ± 11 Bq kg-1) and (1227 ± 318 Bq kg-1) in the rhyolite-dacite samples are greater than the worldwide average. The variation of radioactive bearing minerals observed inside granite faults produced the great amounts of radioactivity perceived in the samples. Calculating radiological risks is used to assess the public's radioactive risk from radionuclides revealed in the studied Rhyolite-dacite samples. The acceptable limit for excess lifetime cancer (ELCR) evaluations has been exceeded. As a result, Rhyolite-dacite are inappropriate for apply in building materials.