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During the summer 2017 ASCENDS/ABoVE airborne science campaign, the NASA Goddard CO2 Sounder lidar overflew smoke plumes from wildfires in the British Columbia, Canada. In the flight path over Vancouver Island on 8 August 2017, the column XCO2 retrievals from the lidar measurements at flight altitudes around 9 km showed an average enhancement of 4 ppm from the wildfires. A comparison of these enhancements with those from the Goddard Global Chemistry Transport model suggested that the modeled CO2 emissions from wildfires were underestimated by more than a factor of 2. A spiral-down validation performed at Moses Lake airport, Washington showed a bias of 0.1 ppm relative to in situ measurements and a standard deviation of 1 ppm in lidar XCO2 retrievals. The results show that future airborne campaigns and spaceborne missions with this type of lidar can improve estimates of CO2 emissions from wildfires and estimates of carbon fluxes globally.
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We report the development of a new type of space lidar specifically designed for missions to small planetary bodies for both topographic mapping and support of sample collection or landing. The instrument is designed to have a wide dynamic range with several operation modes for different mission phases. The laser transmitter consists of a fiber laser that is intensity modulated with a return-to-zero pseudo-noise (RZPN) code. The receiver detects the coded pulse-train by correlating the detected signal with the RZPN kernel. Unlike regular pseudo noise (PN) lidars, the RZPN kernel is set to zero outside laser firing windows, which removes most of the background noise over the receiver integration time. This technique enables the use of low peak-power but high pulse-rate lasers, such as fiber lasers, for long-distance ranging without aliasing. The laser power and the internal gain of the detector can both be adjusted to give a wide measurement dynamic range. The laser modulation code pattern can also be reconfigured in orbit to optimize measurements to different measurement environments. The receiver uses a multi-pixel linear mode photon-counting HgCdTe avalanche photodiode (APD) array with near quantum limited sensitivity at near to mid infrared wavelengths where many fiber lasers and diode lasers operate. The instrument is modular and versatile and can be built mostly with components developed by the optical communication industry.
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Heme is ubiquitous, yet relatively little is known about the maintenance of labile pools of this cofactor, which likely ensures its timely bioavailability for proper cellular function. Quantitative analysis of labile heme is of fundamental importance to understanding how nature preserves access to the diverse chemistry heme enables, while minimizing cellular damage caused by its redox activity. Here, we have developed and characterized a protein-based sensor that undergoes fluorescence quenching upon heme binding. By genetically encoding this sensor in the human malarial parasite, Plasmodium falciparum, we have quantified cytosolic labile heme levels in intact, blood-stage parasites. Our findings indicate that a labile heme pool (â¼1.6 µM) is stably maintained throughout parasite development within red blood cells, even during a period coincident with extensive hemoglobin degradation by the parasite. We also find that the heme-binding antimalarial drug chloroquine specifically increases labile cytosolic heme, indicative of dysregulation of this homeostatic pool that may be a relevant component of the antimalarial activity of this compound class. We propose that use of this technology under various environmental perturbations in P. falciparum can yield quantitative insights into fundamental heme biology.
Assuntos
Técnicas Biossensoriais , Heme/metabolismo , Plasmodium/metabolismo , Animais , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Expressão Gênica , Genes Reporter , Heme/química , Heme/genética , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Microscopia de Fluorescência , Plasmodium/genética , Plasmodium falciparum/genética , Plasmodium falciparum/metabolismo , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
We report results from characterizing the HgCdTe avalanche photodiode (APD) arrays developed for lidar at infrared wavelengths by using the high density vertically integrated photodiodes (HDVIP®) technique. The results show >90% quantum efficiencies between 0.8 µm and the cut-off wavelength, >600 APD gain, near unity excess noise factor, 6-10 MHz electrical bandwidth and <0.5 fW/Hz1/2 noise equivalent power (NEP). The detectors provide linear analog output with a dynamic range of 2-3 orders of magnitude at a fixed APD gain without averaging, and over 5 orders of magnitude by adjusting the APD gain settings. They have been used successfully in airborne CO2 and CH4 integrated path differential absorption (IPDA) lidar as precursors for use in space lidar.
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NASA's Ice, Cloud, and land Elevation Satellite (ICESat), which operated between 2003 and 2009, made the first satellite-based global lidar measurement of Earth's ice sheet elevations, sea-ice thickness and vegetation canopy structure. The primary instrument on ICESat was the Geoscience Laser Altimeter System (GLAS), which measured the distance from the spacecraft to Earth's surface via the roundtrip travel time of individual laser pulses. GLAS utilized pulsed lasers and a direct detection receiver consisting of a silicon avalanche photodiode (Si APD) and a waveform digitizer. Early in the mission, the peak power of the received signal from snow and ice surfaces was found to span a wider dynamic range than planned, often exceeding the linear dynamic range of the GLAS 1064-nm detector assembly. The resulting saturation of the receiver distorted the recorded signal and resulted in range biases as large as ~50 cm for ice and snow-covered surfaces. We developed a correction for this "saturation range bias" based on laboratory tests using a spare flight detector, and refined the correction by comparing GLAS elevation estimates to those derived from Global Positioning System (GPS) surveys over the calibration site at the salar de Uyuni, Bolivia. Applying the saturation correction largely eliminated the range bias due to receiver saturation for affected ICESat measurements over Uyuni and significantly reduced the discrepancies at orbit crossovers located on flat regions of the Antarctic ice sheet.
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We report on an airborne demonstration of atmospheric oxygen optical depth measurements with an IPDA lidar using a fiber-based laser system and a photon counting detector. Accurate knowledge of atmospheric temperature and pressure is required for NASA's Active Sensing of CO2 Emissions over Nights, Days, and Seasons (ASCENDS) space mission, and climate modeling studies. The lidar uses a doubled erbium-doped fiber amplifier and single photon-counting detector to measure oxygen absorption at 765 nm. Our results show good agreement between the experimentally derived differential optical depth measurements with the theoretical predictions for aircraft altitudes from 3 to 13 km.
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We report on airborne CO(2) column absorption measurements made in 2009 with a pulsed direct-detection lidar operating at 1572.33 nm and utilizing the integrated path differential absorption technique. We demonstrated these at different altitudes from an aircraft in July and August in flights over four locations in the central and eastern United States. The results show clear CO(2) line shape and absorption signals, which follow the expected changes with aircraft altitude from 3 to 13 km. The lidar measurement statistics were also calculated for each flight as a function of altitude. The optical depth varied nearly linearly with altitude, consistent with calculations based on atmospheric models. The scatter in the optical depth measurements varied with aircraft altitude as expected, and the median measurement precisions for the column varied from 0.9 to 1.2 ppm. The altitude range with the lowest scatter was 8-10 km, and the majority of measurements for the column within it had precisions between 0.2 and 0.9 ppm.
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We use theoretical models to compare the receiver signal to noise ratio (SNR) vs. average rate of detected signal photons for an integrated path differential absorption (IPDA) lidar using coherent detection with continuous wave (CW) lasers and direct detection with sine-wave and pulse modulations. The results show the coherent IPDA lidar has high receiver gain and narrow bandwidth to overcome the effects of detector circuit noise and background light, but the actual receiver performance can be limited by the coherent mixing efficiency, speckle and other factors. For direct detection, using sine-wave modulation allows the use of a low peak power laser transmitter and synchronous detection. The pulse modulation technique requires higher laser peak powers but is more efficient than sine-wave modulation in terms of average detected signal photon rate required to achieve a given receiver SNR. We also conducted experiments for the direct detection cases and the results agreed well with theory.
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We report airborne measurements of the column abundance of atmospheric methane made over an altitude range of 3-11 km using a direct detection integrated-path differential-absorption lidar with a pulsed laser emitting at 1651 nm. The laser transmitter was a tunable, seeded optical parametric amplifier pumped by a Nd:YAG laser, and the receiver used a photomultiplier detector and photon-counting electronics. The results follow the expected changes with aircraft altitude, and the measured line shapes and optical depths show good agreement with theoretical calculations.
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We demonstrate a wavelength-locked laser source that rapidly steps through six wavelengths distributed across a 1572.335 nm carbon dioxide (CO(2)) absorption line to allow precise measurements of atmospheric CO(2) absorption. A distributed-feedback laser diode (DFB-LD) was frequency-locked to the CO(2) line center by using a frequency modulation technique, limiting its peak-to-peak frequency drift to 0.3 MHz at 0.8 s averaging time over 72 hours. Four online DFB-LDs were then offset locked to this laser using phase-locked loops, retaining virtually the same absolute frequency stability. These online and two offline DFB-LDs were subsequently amplitude switched and combined. This produced a precise wavelength-stepped laser pulse train, to be amplified for CO(2) measurements.
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Plasmodium knowlesi is a zoonotic parasite transmitted from macaques causing malaria in humans in Southeast Asia. Plasmodium parasites bind to red blood cell (RBC) surface receptors, many of which are sialylated. While macaques synthesize the sialic acid variant N-glycolylneuraminic acid (Neu5Gc), humans cannot because of a mutation in the enzyme CMAH that converts N-acetylneuraminic acid (Neu5Ac) to Neu5Gc. Here we reconstitute CMAH in human RBCs for the reintroduction of Neu5Gc, which results in enhancement of P. knowlesi invasion. We show that two P. knowlesi invasion ligands, PkDBPß and PkDBPγ, bind specifically to Neu5Gc-containing receptors. A human-adapted P. knowlesi line invades human RBCs independently of Neu5Gc, with duplication of the sialic acid-independent invasion ligand, PkDBPα and loss of PkDBPγ. Our results suggest that absence of Neu5Gc on human RBCs limits P. knowlesi invasion, but that parasites may evolve to invade human RBCs through the use of sialic acid-independent pathways.
Assuntos
Malária/prevenção & controle , Ácido N-Acetilneuramínico/genética , Plasmodium knowlesi/patogenicidade , Zoonoses/parasitologia , Animais , Eritrócitos/metabolismo , Eritrócitos/parasitologia , Genoma de Protozoário , Células HEK293 , Humanos , Oxigenases de Função Mista/genética , Ácido N-Acetilneuramínico/biossíntese , Ácido N-Acetilneuramínico/química , Ácidos Neuramínicos/química , Ácidos Neuramínicos/metabolismo , Plasmodium knowlesi/genética , Proteínas de Protozoários/química , Proteínas de Protozoários/genética , Zoonoses/prevenção & controle , Zoonoses/transmissãoRESUMO
A technique was developed to compute the radiance of the scene viewed by the optical receiver of the Mars Orbiter Laser Altimeter. The technique used the detection threshold and the false detection rate of the receiver to provide a passive radiometry measurement of Mars at the 1064 nm wavelength over a 2 nm bandwidth and subkilometer spatial resolution in addition to the altimetry and active radiometry measurements. The passive radiometry measurement is shown to have a 2% or better precision and has been stable over several Martian years. We describe the principle of operation of the instrument and its calibration and assess its performance from sample orbital measurements.
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The design and preliminary tests of an automated differential absorption lidar (DIAL) that profiles water vapor in the lower troposphere are presented. The instrument, named CODI (for compact DIAL), has been developed to be eye safe, low cost, weatherproof, and portable. The lidar design and its unattended operation are described. Nighttime intercomparisons with in situ sensors and a radiosonde are shown. Desired improvements to the lidar, including a more powerful laser, are also discussed.