Prevalence and molecular characterization of Mycobacterium tuberculosis complex in cattle and humans, Maiduguri, Borno state, Nigeria: a cross-sectional study.

INTRODUCTION: Globally, the highest burden of bovine and human tuberculosis resides in Africa and Asia. Tuberculosis (TB) is the second leading single infectious killer after severe acute respiratory syndrome corona virus-2 (SARSCOV-2). Bovine TB remains a treat to wild and domesticated animals, humans and hinders international trade in endemic countries like Nigeria. We aimed at determining the prevalence of bovine and human tuberculosis, and the spoligotypes of Mycobacterium tuberculosis complex in cattle and humans in Maiduguri. METHODS: We conducted a cross sectional study on bovine and human tuberculosis in Maiduguri, Borno state. We calculated sample size using the method of Thrusfield. Lesions suggestive of TB from 160 slaughtered cattle were obtained from Maiduguri Central Abattoir. Sputum samples from humans; 82 abattoir workers and 147 suspected TB patients from hospitals/clinics were obtained. Lesions and sputum samples were cultured for the isolation of Mycobacterium spp. Positive cultures were subjected genus typing, deletion analysis and selected isolates were spoligotyped. Data was analysed using SPSS VERSION 16.0. RESULTS: Prevalence of 32.5% (52/160) was obtained in cattle. Damboa local government area (LGA), where majority of the infected animals were obtained from had 35.5% bTB prevalence. All categories analysed (breed, age, sex, body conformation and score) had P-values that were not significant (P > 0.05). Sputum culture revealed a prevalence of 3.7% (3/82) from abattoir workers and 12.2% from hospitals/clinics. A significant P-value (0.03) was obtained when positive culture from abattoir and that of hospitals/clinics were compared. Out of the 52 culture positive isolates obtained from cattle, 26 (50%) belonged to M. tuberculosis complex (MTC) and 17/26 (65.4%) were characterized as M. bovis. In humans, 7/12 (58.3%) MTC obtained were characterized as M. tuberculosis. Spoligotyping revealed SB0944 and SB1025 in cattle, while SIT838, SIT61 of LAM10_CAM and SIT1054, SIT46 of Haarlem (H) families were obtained from humans. CONCLUSIONS: Cattle in Damboa LGA need to be screened for bTB as majority of the infected animals were brought from there. Our findings revealed the presence of SB0944 and SB1025 spoligotypes from cattle in Borno state. We isolated M. tuberculosis strain of the H family mainly domiciled in Europe from humans.

Reverse zoonotic tuberculosis transmission from an emerging Uganda I strain between pastoralists and cattle in South-Eastern Nigeria.

BACKGROUND: Tuberculosis remains a major public health challenge globally with increasing risks for inter-transmission between pastoralists and cattle in Nigeria. This study was aimed at using molecular tools to establish zoonotic transmission of tuberculosis between pastoralists and their cattle in Ebonyi State, Nigeria. Sputum (n = 149) and milk (n = 144) samples from pastoralists and cattle, respectively were screened on the assumption of subclinical infections considering unguarded human-livestock interactions. Isolates obtained were analysed using deletion typing, spoligotyping and 24-Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats (MIRU-VNTR). RESULTS: Fifty-four MTC were confirmed by deletion typing and were differentiated accordingly (M. tuberculosis: pastoralists =42, cattle = 2; M. bovis: pastoralists =1; M. africanum: pastoralists =9). Spoligotyping indicated 59.2% Uganda I/SIT46 (pastoralists =28; cattle = 1), 16.3% Latin American Mediterranean/SIT61 (pastoralists =8), 2.0% T/SIT53 (pastoralists =1) strains of M. tuberculosis and new strains of M. bovis and M. africanum. The 24-MIRU-VNTR of selected predominant cluster isolates shared by cattle and pastoralists (Uganda I/SIT46: pastoralists =9; cattle = 1) showed the same number of copies at each of the repetitive loci. CONCLUSIONS: Mycobacterium bovis was confirmed in humans and a reverse zoonotic tuberculosis transmission from an emerging Uganda I M. tuberculosis strain between pastoralists and cattle in Nigeria evidenced by MIRU-VNTR. Using molecular tools will help mitigate disease burden through informed epidemiological insights.

Prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria.

Using a cross-sectional survey, we determined the prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria. Antibodies to Brucella species in serum samples were tested using the Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (cELISA); for milk, the milk ring test (MRT) and indirect-ELISA (i-ELISA) were used. Questionnaire was administered to cattle herdsmen to determine factors predisposing the animals to bovine brucellosis. Data were analyzed using STATA 12. From 513 serum and 635 milk samples tested among 120 herds, overall animal-level prevalence of 10.1% (95% CI 7.5-12.7%) and 20.2% (95% CI 17.1-23.3%) were recorded by RBT and MRT, respectively; while 9.4% (95% CI 6.9-11.9%) and 17.8% (95% CI 14.8-20.8%) were obtained using cELISA and i-ELISA, respectively. In all, from the 120 herds tested, 29.2% and 43.3% were positive by RBT and MRT, respectively. Multivariable logistic regression revealed that herd location (OR = 8.12, 95% CI 1.68-38.90) and improper disposal of placenta/fetus (OR = 17.33, 95% CI 4.81-62.33) were predictors for a seropositive herd using RBT; while herd location (OR = 5.13, 95% CI 1.27-20.28), large herd size (OR = 2.62, 95% CI 1.15-5.85), and occurrence of abortion for a year or more (OR = 4.62, 95% CI 1.53-13.71) were predictors of seropositivity to antibodies to Brucella spp. using MRT. We found high prevalence of brucellosis in cattle herds under extensive management system in southwestern Nigeria. Urgent and coordinated control strategies are required to mitigate this problem.

Seroprevalence and associated factors of brucellosis and Q-fever in cattle from Ibarapa area, Oyo State, South-western Nigeria.

INTRODUCTION: a cross-sectional study was conducted to determine the seroprevalence and associated factors of brucellosis and Q-fever among cattle in a rural setting in Oyo State, Nigeria. METHODS: one hundred and fourty nine serum samples (24 males; 125 female) from 16 cattle herds were collected and screened. The Rose Bengal Plate test (RBPT) and competitive Enzyme-Linked Immunosorbent Assay (cELISA) were used for brucellosis while indirect Enzyme-Linked Immunosorbent Assay (iELISA) was used for Q-fever. Further, a checklist was used to collect data on cattle sampled. Data were analyzed using STATA 12. RESULTS: serum analysis revealed that 11.4% (17/149) and 6.7% (10/149) were seropositive by RBPT and cELISA respectively for brucellosis, while 23.5% (35/149) were seropositive by iELISA for Q-fever. A significant association was detected between cattle age (OR=27.7; 95% CI: 2.34-449.86), herd size (OR=10.53; 95% CI: 1.85-60.53) and seropositivity to Brucella infection. Also, there was a significant association between breed (OR=6.69; 95% CI: 1.7-28.74), herd size (OR=4.25; 95% CI: 1.31-13.85) of cattle and seropositivity to Coxiella burnetii infection. Importantly, the only significant associated factor to cattle herd seropositivity to Brucella and C. burnetii infections was the method of handling aborted foetuses. CONCLUSION: the study revealed that brucellosis and Q-fever are prevalent among cattle in the study area. Thus, there is a need for further studies to provide better insight into the epidemiology of both diseases and particularly Q-fever. This becomes imperative in the study area and generally in Nigeria given the dearth of information about the diseases in pastoralist communities who are at grave risk of infection at the human-animal-ecosystem interface.

Knowledge and practices about zoonotic tuberculosis prevention and associated determinants amongst livestock workers in Nigeria; 2015.

Zoonotic tuberculosis (TB) is a significant public health disease, but has long been neglected. Information on knowledge and practices of its prevention and influencing factors amongst occupationally exposed individuals is required for designing all-inclusive, informed control programmes. We investigated knowledge and practices related to zoonotic TB prevention and associated determinants amongst herdsmen and abattoir workers in south-western, south-eastern and north-western Nigeria using semi-structured questionnaire. Data were analysed with STATA 12. A total of 510 respondents (196 herdsmen; 314 abattoir workers) participated in the survey, of which 58.6% and 46.9% respectively were knowledgeable and demonstrated good practices about zoonotic TB prevention. Almost 60% knew that zoonotic TB transmission was preventable and 49.8% knew transmission could be through consumption of infected animal products. However, only 16.7% knew the disease could be transmitted by aerosol. Just 49.4% sought medical check-up when ill, 37.8% used protective clothing and only 29.2% usually condemned TB infected cattle. Respondents with post-primary education were about three times more likely to be knowledgeable (OR = 2.70, 95%CI: 1.68-4.33) and two times more likely to demonstrate good practice (OR = 2.23, 95%CI: 1.45-3.42) than those without formal education. Similarly, abattoir workers were about 6.4 times more likely to be knowledgeable (OR: 6.39, 95%CI: 4.31-9.47) and two times more likely to demonstrate good practice (OR: 2.03, 95%CI: 1.40-2.92) than the herdsmen. There were important knowledge gaps with poor practices about zoonotic TB prevention amongst livestock workers in Nigeria. Strong predictors of knowledge and practice were being an abattoir worker and having post-primary education. Well-designed grassroots enlightenment programmes addressing modes of transmission, handling infected cattle and seeking medical check-up are urgently needed among high risk settings considering the recently launched Road Map for Zoonotic Tuberculosis which resonates that every tuberculosis case counts towards 2030 End-TB Strategy.

Prevalence of bovine brucellosis in slaughtered cattle and barriers to better protection of abattoir workers in Ibadan, South-Western Nigeria.

INTRODUCTION: Brucellosis is a neglected zoonosis of public health importance. This study was conducted to determine the prevalence and risk factors of brucellosis among slaughtered cattle as well as challenges to the protection of abattoir workers in Nigeria. METHODS: A slaughterhouse study was conducted in a major abattoir in Ibadan from March to August, 2013. To diagnose brucellosis, serum samples from 1,241 slaughtered cattle were tested using Rose-Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (cELISA); again, 57 milk samples were tested with milk ring test (MRT) and indirect ELISA (iELISA). Furthermore, a survey on the usage of personal protective equipment (PPE) and challenges to its use by abattoir workers was done. Data were analysed using Stata 12. RESULTS: Seroprevalence by RBT was 7.8%; 77.3% (75/97) of these were corroborated by cELISA. Prevalence in milk samples by MRT and indirect ELISA were 33.3% and 3.5%, respectively. Sex (OR: 2.5; 95%CI:1.3-4.5) was the factor significantly associated with Brucella seropositivity. None of the abattoir workers used standard protective overalls; while, 99.6% of the meat handlers and 84.1% of the butchers worked barefoot. Most of the workers (75.7%) wore no protective gloves. The respondents agreed that provision of free PPE and sanctions against non-users would encourage its use. CONCLUSION: Our findings indicate moderate prevalence (7.8%) of bovine brucellosis with sex of cattle being a risk factor. A notable barrier to better protection of abattoir workers against brucellosis is perceived inconvenience arising from use of gloves. Therefore, preventive and control measures against brucellosis must include education and use of PPE among abattoir workers.

Sero-epidemiological survey and risk factors associated with brucellosis in dogs in south-western Nigeria.

INTRODUCTION: In Nigeria, there is limited information on brucellosis particularly in dogs, despite its public health implications. We undertook a sero-epidemiological survey of brucellosis in dogs to determine the prevalence of the disease and associated risk factors for its occurrence in Nigeria. METHODS: A cross-sectional study was conducted to screen dogs in south-western Nigeria for antibodies to Brucella sp using the rapid slide agglutination test (RSA) and Rose Bengal test (RBT), with positive samples confirmed respectively by serum agglutination test (SAT) and competitive enzyme linked immunosorbent assay (cELISA). Data were analyzed with STATA-12. RESULTS: From the 739 dog sera tested, 81 (10.96%) were positive by RSA and 94 (12.72%) by RBT; these were corroborated with SAT (4/81; 4.94%) and cELISA (1/94; 1.06%), respectively. Logistic regression identified location (OR=0.04; 95% CI: 0.02-0.09), breed (OR=1.71; 95% CI: 1.34-2.19), age (OR=0.10; 95% CI: 0.04-0.30) and management system (OR=8.51; 95% CI: 1.07-68.05) as risk factors for Brucella infection by RSA. However, location (OR=10.83; 95% CI: 5.48-21.39) and history of infertility (OR=2.62; 95% CI: 1.41-4.84) were identified as risk factors using RBT. CONCLUSION: Given the 10.96% to 12.72% seroprevalence of brucellosis recorded in this study, we advocate control of the disease in dogs, and public health education for those at risk of infection. Again, further studies are required to elucidate the role of dogs in the epidemiology of brucellosis in Nigeria considering the conducive human-animal interface and ecological factors responsible for the transmission of the disease.

Food Safety Training Is Associated with Improved Knowledge and Behaviours among Foodservice Establishments' Workers.

Though several studies have evaluated the association between food safety training and behavior, little has investigated different training components in association with food handlers' performance. Foodservice workers (N = 211) with at least two years' experience were willing to participate and were selected from major foodservice establishments in Ibadan, southwestern Nigeria, and completed a survey to evaluate the association between training, training area, duration, and refresher training and food safety knowledge and practices. We observed an association between training and knowledge (P = 0.000) as well as practices (P = 0.05) of food safety while different training areas contributed similarly to food handlers' knowledge (P = 0.17) and practices (P = 0.08). However, there was a significant decline in knowledge (P = 0.01) and practices (P = 0.001) with an increase in training duration. Furthermore, foodservice employees with refresher training demonstrated significantly higher knowledge (P = 0.000) and practice (P = 0.003) levels than those without, being about 45 and 14 times more likely to, respectively, improve their knowledge (OR = 45; 95% CI: 3.47-584.34) and practice (OR = 13.5; 95% CI: 2.01-90.69). Researchers should always consider varying training components before making assertions regarding effectiveness of training on foodservice workers' behaviour.

Gastrointestinal Helminths in Slaughtered Cattle in Ibadan, South-Western Nigeria.

As part of an ongoing project to investigate the epidemiology of gastrointestinal helminths of cattle in Nigeria, we carried out a systematic random sampling of cattle slaughtered in a major abattoir in Ibadan, south-western Nigeria. Using sedimentation and floatation methods, we analyzed fecal samples from 397 animals between March and May 2013. Overall, 163 (41.6%) of the animals had at least one gastrointestinal helminth egg, comprising a total of eight helminths from different genera (i.e., four nematodes, three trematodes, and one cestode), with nematode infection being the highest (71.54%). In addition, eggs of four helminths of zoonotic importance were also obtained. Among the cattle examined, the Bunaji breed was the most infected (46%; 69/150). Furthermore, female animals (OR = 1.1; 95% CI: 0.60-1.84) and animals with moderate body condition (OR = 1.2; 95% CI: 0.80-1.79) are more likely to be positive to helminth infection. Our findings reveal that there were helminth infections of both zoonotic and socioeconomic importance among the cattle screened. Considering the impact of the infections on animal production and public health, we advocate that effective prophylactic measures be adopted as a first step to curtail helminth infections of cattle in Nigeria.