RESUMO
Studies on ribosome biosynthesis in developing Xenopus oocytes and embryos, and after microinjection of cloned ribosomal-protein genes, have revealed that the synthesis of ribosomal proteins (r-proteins) is controlled by two types of regulation: (1) a post-transcriptional regulation, operated by feedback of the r-proteins themselves, controls processing and stability of r-protein transcripts and thus the amount of the corresponding mRNA present in the cell; and (2) a translational regulation controls the efficiency of utilization of r-protein mRNA (rp-mRNA) in response to the cellular needs for new ribosomes.
Assuntos
Proteínas Ribossômicas/biossíntese , Ribossomos/metabolismo , Xenopus/genética , AnimaisRESUMO
During Xenopus development, the synthesis of ribosomal proteins is regulated at the translational level. To identify the region of the ribosomal protein mRNAs responsible for their typical translational behavior, we constructed a fused gene in which the upstream sequences (promoter) and the 5' untranslated sequence (first exon) of the gene coding for Xenopus ribosomal protein S19 were joined to the coding portion of the procaryotic chloramphenicol acetyltransferase (CAT) gene deleted of its own 5' untranslated region. This fused gene was introduced in vivo by microinjection into Xenopus fertilized eggs, and its activity was monitored during embryogenesis. By analyzing the pattern of appearance of CAT activity and the distribution of the S19-CAT mRNA between polysomes and messenger ribonucleoproteins, it was concluded that the 35-nucleotide-long 5' untranslated region of the S19 mRNA is able to confer to the fused S19-CAT mRNA the translational behavior typical of ribosomal proteins during Xenopus embryo development.
Assuntos
Embrião não Mamífero/fisiologia , Regulação da Expressão Gênica , Genes , Biossíntese de Proteínas , Proteínas Ribossômicas/genética , Animais , Sequência de Bases , Clonagem Molecular , DNA/genética , DNA/isolamento & purificação , Feminino , Dados de Sequência Molecular , Polirribossomos/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/isolamento & purificação , Mapeamento por Restrição , Ribonucleoproteínas/metabolismo , Proteínas Ribossômicas/metabolismo , Transcrição Gênica , Xenopus laevis , Zigoto/fisiologiaRESUMO
Autoantibodies against U3 small nuclear ribonucleoprotein are associated with scleroderma autoimmune disease. They were shown to react with fibrillarin, a 34- to 36-kilodalton protein that has been detected in all eukaryotes tested from humans to yeasts. We isolated a 1.6-kilobase cDNA encoding fibrillarin from a Xenopus laevis cDNA library. The protein contains a 79-residue-long Gly-Arg-rich domain in its N-terminal region and a putative RNA-binding domain with ribonucleoprotein consensus sequence in its central portion. This is the first report of cloning of fibrillarin, and the deduced protein sequence is in agreement with the involvement of the protein in a ribonucleoprotein particle.
Assuntos
Autoantígenos/imunologia , Proteínas Cromossômicas não Histona/genética , RNA Nuclear Pequeno/metabolismo , Ribonucleoproteínas/genética , Xenopus laevis/genética , Sequência de Aminoácidos , Animais , Doenças Autoimunes/imunologia , Sequência de Bases , Sítios de Ligação , Northern Blotting , Clonagem Molecular , Dados de Sequência Molecular , Proteínas Recombinantes , Ribonucleoproteínas Nucleares PequenasRESUMO
The nucleolar localization elements (NoLEs) of U17 small nucleolar RNA (snoRNA), which is essential for rRNA processing and belongs to the box H/ACA snoRNA family, were analyzed by fluorescence microscopy. Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion of stems 1, 2, and 4 of U17 snoRNA reduced but did not prevent nucleolar localization. The deletion of stem 3 had no adverse effect. Therefore, the hairpins of the hairpin-hinge-hairpin-tail structure formed by these stems are not absolutely critical for nucleolar localization of U17, nor are sequences within stems 1, 3, and 4, which may tether U17 to the rRNA precursor by base pairing. In contrast, box H and box ACA are major NoLEs; their combined substitution or deletion abolished nucleolar localization of U17 snoRNA. Mutation of just box H or just the box ACA region alone did not fully abolish the nucleolar localization of U17. This indicates that the NoLEs of the box H/ACA snoRNA family function differently from the bipartite NoLEs (conserved boxes C and D) of box C/D snoRNAs, where mutation of either box alone prevents nucleolar localization.
Assuntos
RNA Nucleolar Pequeno/genética , Animais , Sequência de Bases , Nucléolo Celular/metabolismo , Sequência Conservada , Evolução Molecular , Microinjeções , Microscopia de Fluorescência , Dados de Sequência Molecular , Mutação , Conformação de Ácido Nucleico , Oócitos , RNA Nuclear/genética , RNA Ribossômico/metabolismo , Deleção de Sequência , XenopusRESUMO
In vertebrates, the mRNAs encoding ribosomal proteins, as well as other proteins implicated in translation, are characterized by a 5'-untranslated region (5'-UTR), including a stretch of pyrimidines at the 5'-end. The 5'-terminal oligopyrimidine (5'-TOP) sequence, which is involved in the growth-dependent translational regulation characteristic of this class of genes (so-called TOP genes), has been shown to specifically bind the La protein in vitro, suggesting that La might be implicated in translational regulation in vivo. In order to substantiate this hypothesis, we have examined the effect of La on TOP mRNA translational control in both stable and transient transfection experiments. In particular we have constructed and analyzed three stably transfected Xenopus cell lines inducible for overexpression of wild-type La or of putative dominant negative mutated forms. Moreover, La-expressing plasmids have been transiently co-transfected together with a plasmid expressing a reporter TOP mRNA in a human cell line. Our results suggest that in vivo La protein plays a positive role in the translation of TOP mRNA. They also suggest that the function of La is to counteract translational repression exerted by a negative factor, possibly cellular nucleic acid binding protein (CNBP), which has been previously shown to bind the 5'-UTR downstream from the 5'-TOP sequence.
Assuntos
Regiões 5' não Traduzidas/genética , Autoantígenos/fisiologia , Biossíntese de Proteínas/efeitos dos fármacos , Pirimidinas , RNA Mensageiro/genética , Ribonucleoproteínas/fisiologia , Animais , Autoantígenos/genética , Autoantígenos/metabolismo , Linhagem Celular , Expressão Gênica/efeitos dos fármacos , Pirimidinas/metabolismo , Ribonucleoproteínas/genética , Ribonucleoproteínas/metabolismo , Transfecção , Xenopus , Antígeno SS-BRESUMO
Mutations in the 5' UTR which cause increment/decrement of translation efficiency have been recently described as a novel molecular mechanism of disease. Alterations in the consensus sequence for the translation initiation may promote context-dependent leaky scanning of ribosomes and/or initiation from a downstream AUG codon. Initiation of translation from a downstream in-frame AUG codon in BRCA1 gene was recently identified in normal cells and possibly in breast cancer. Here we present further insight into BRCA1 translational pathophysiology investigating the role of the canonical structure of the initiation consensus sequence of BRCA1. We have analysed the effect of a somatic point mutation (117 G>C) in position -3 with respect to the AUG of the BRCA1 gene, identified in a highly aggressive sporadic breast cancer. We constructed chimeric genes encoding the luciferase reporter sequence downstream of the wild type or the mutated BRCA1 5'UTR. These transcripts were tested for their activity in in vitro and in vivo systems. In in vitro transcription/translation assays the estimated translation efficiency of the construct with the mutated BRCA1 5'UTR was 30-50% lower than that with the wild type BRCA1 5'UTR. The same chimeric genes were analysed for their expression in vivo by transient transfection in human cells. While the two constructs were equally transcribed, the plasmid carrying the mutated sequence produced 70% less luciferase activity compared to the wild type sequence. Finally, to obtain a direct evaluation on translational efficiency in vivo, we analysed mRNA translation on translationally active and non-active ribosomes separated from transfected cells. Mutant mRNA was partially localized in subpolysomal particles analytically confirming a polysome recruitment defect. Thus, characterization of BRCA1 5'UTR and translation efficiency seems to provide new insight into BRCA1 role in breast and ovarian cancer pathogenesis.
Assuntos
Regiões 5' não Traduzidas/genética , Neoplasias da Mama/genética , Carcinoma/genética , Genes BRCA1 , Mutação , Biossíntese de Proteínas , Bacteriófago T7/genética , Linhagem Celular , Sistema Livre de Células , Sequência Consenso , Feminino , Genes Reporter , Genes Sintéticos , Humanos , Rim , Luciferases/biossíntese , Luciferases/genética , Iniciação Traducional da Cadeia Peptídica/genética , Regiões Promotoras Genéticas , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genéticaRESUMO
The presence of specific mRNAs in dendrites and at synapses is well established, but a direct and reliable demonstration that they are associated with polysomes is still missing. To address this point we analyzed the polysomal association of the mRNAs for the alpha-subunit of Ca(2+)/calmodulin-dependent protein kinase II (alpha-CaMKII), for type 1 inositol 1,4,5-trisphosphate receptor (InsP3R1) and for the activity-regulated cytoskeleton-associated protein (Arc) in a synaptosomal preparation devoid of contaminating material from neuronal and glial perikarya. We show that a fraction of alpha-CaMKII, InsP3R1, and Arc mRNAs present in synaptosomes is indeed associated with polysomes. Moreover, we show that polysomal association of alpha-CaMKII mRNA, but not InsP3R1 and Arc mRNAs, increases with depolarization of the synaptosomal membrane. Finally, we show that the synthesis of alpha-CaMKII protein increases with stimulation. Dendritic mRNA recruitment onto polysomes in response to synaptic stimulation might represent one of the mechanisms underlying the processes of learning and memory.
Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Polirribossomos/metabolismo , RNA Mensageiro/metabolismo , Sinaptossomos/metabolismo , Animais , Encéfalo/ultraestrutura , Canais de Cálcio/metabolismo , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina , Proteínas do Citoesqueleto/metabolismo , Técnicas In Vitro , Receptores de Inositol 1,4,5-Trifosfato , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Eletrônica , Proteínas do Tecido Nervoso/metabolismo , Polirribossomos/enzimologia , Cloreto de Potássio/farmacologia , Receptores Citoplasmáticos e Nucleares/metabolismo , Estimulação Química , Sinaptossomos/enzimologiaRESUMO
The cloning and sequencing of a cDNA for human ribosomal protein L4 is reported. The corresponding mRNA has a very short 5' untranslated region initiating with a sequence of 12 pyrimidines, characteristic of all vertebrate ribosomal protein mRNAs. The deduced amino acid sequence shows that human ribosomal protein L4 has 425 amino acid residues and a calculated molecular mass of 47,821 Da. Comparison with the homologous counterparts of Xenopus, Drosophila and yeast shows that this protein has a very conserved amino-terminus region and an extremely divergent carboxyl-terminus portion.
Assuntos
Proteínas Ribossômicas/biossíntese , Proteínas Ribossômicas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar/química , DNA Complementar/metabolismo , Drosophila melanogaster/genética , Humanos , Dados de Sequência Molecular , Saccharomyces cerevisiae/genética , Homologia de Sequência de Aminoácidos , Xenopus laevisRESUMO
The activity of antisera against ribonucleoproteins containing U1 small nuclear RNA (Sm and RNP) has been analysed on pol II transcripts in an in vivo system. Xenopus laevis ribosomal protein gene transcripts are accumulated in the form of precursor RNA when either of the two kinds of antisera are injected into the germinal vesicles of X. laevis oocytes before the injection of purified L1 and L14 ribosomal protein genes. No effect on the accumulation of mature histone mRNA is detected when X. laevis histone genes are injected together with the RNP antiserum. These results strongly suggest that U1-RNP complexes play an essential role in intron removal in vivo.
Assuntos
Soros Imunes , Splicing de RNA , RNA Ribossômico/genética , RNA/imunologia , Ribonucleoproteínas/imunologia , Proteínas Ribossômicas/genética , Animais , Hibridização de Ácido Nucleico , RNA Nuclear Pequeno , Ribonucleoproteínas Nucleares Pequenas , Transcrição Gênica , Xenopus laevisRESUMO
The expression of two Xenopus laevis ribosomal protein genes (L1 and L14) has been analysed by microinjection of the cloned genomic sequences into frog oocyte nuclei. While the injection of the L14 gene causes the accumulation of the corresponding protein in large excess with respect to that synthesized endogenously, the L1 gene does not. Analysis of the RNA shows that both genes are actively transcribed. The seven-intron-containing L14 transcript is completely processed to a mature form, while two out of nine intron sequences persist in the L1 transcript. This precursor RNA is confined to the nucleus; its accumulation is due to a specific block of splicing operating at the level of two defined introns and not to saturation of the processing apparatus of the oocyte. The different behaviour of the two genes may reflect different mechanisms of regulation which, in the case of the L1 gene, could operate at the level of splicing.
Assuntos
Processamento Pós-Transcricional do RNA , RNA/metabolismo , Proteínas Ribossômicas/genética , Animais , Sequência de Bases , Clonagem Molecular , DNA , Eletroforese em Gel de Poliacrilamida , Regulação da Expressão Gênica , Microscopia Eletrônica , Hibridização de Ácido Nucleico , Biossíntese de Proteínas , Splicing de RNA , Transcrição Gênica , Xenopus laevisRESUMO
Recombinant clones specific for ribosomal protein (r-protein) S8 have been isolated from a Xenopus laevis cDNA bank. Sequence analysis shows that they are of two types, derived from two different gene copies originating from gene duplication. The two cDNAs differ in several silent sites and code for the same S8 protein whose complete amino acid sequence has been derived. Sequence comparison of S8 mRNAs with those for other X. laevis r-proteins, has revealed interesting similarities in the 5' and 3' untranslated regions. These could be involved in r-protein synthesis regulation which we have previously shown to occur mainly at post-transcriptional and translational levels.
Assuntos
DNA/isolamento & purificação , RNA Mensageiro/genética , Proteínas Ribossômicas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , DNA/genética , Enzimas de Restrição do DNA , Genes , Dados de Sequência Molecular , Xenopus laevisRESUMO
The genes coding for ribosomal proteins (r-proteins) in Xenopus laevis have a stretch of about 20 pyrimidines (Y) at their 5' end, in the middle of which is localized the main transcription start point (tsp). To obtain information about its possible functional significance, we have introduced by site-directed mutagenesis one or more purines at various positions within the oligo(Y) tract present at the 5' end of the gene coding for r-protein S19 of X. laevis. The effect of these mutations on transcription and translation of a reporter-coding sequence has been evaluated by DNA microinjection in X. laevis oocytes. We show that an uninterrupted stretch of pyrimidines is not necessary for efficient transcription and translation of the reporter gene in the X. laevis oocyte. This finding does not exclude the possibility that this sequence is involved in transcription and/or translation regulation in some developmental situations different from oogenesis.
Assuntos
Oócitos/metabolismo , Pirimidinas , Proteínas Ribossômicas/genética , Transcrição Gênica , Animais , Sequência de Bases , Clonagem Molecular , DNA , Regulação da Expressão Gênica/genética , Microinjeções , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Xenopus laevisRESUMO
A cDNA clone was isolated from a Xenopus laevis embryo library and sequenced. Primer extension experiments indicated the full-length nature of the insert and the encoded product was identified on a two dimensional gel as ribosomal protein (r-protein) L22. The 510-bp L22 cDNA sequence presents short untranslated regions and a 5'-end polypyrimidine tract found in all other vertebrate r-protein mRNA (rp mRNA) so far analyzed. Both the nucleotide (nt) and the deduced amino acid (aa) sequences have been compared with the homologous sequences from other species. The L22 nt sequence is about 70% similar to the mammalian L27a rp mRNA and about 60% homologous to the Drosophila, Tetrahymena and yeast corresponding mRNAs. The 148-aa sequence presents a higher conservation, being 90% similar to the mammalian sequence and more than 70% to the other species. Expression analysis showed that, both during X. laevis embryogenesis and in X. laevis cultured cells during growth-rate changes, L22 synthesis is translationally regulated. Therefore X. laevis L22 mRNA is a new example of the correlation between the polypyrimidine terminal tract and the translational regulation observed in other rp mRNAs.
Assuntos
Proteínas de Drosophila , Biossíntese de Proteínas , Proteínas de Ligação a RNA/genética , Proteínas Ribossômicas/genética , Proteínas de Xenopus , Xenopus laevis/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sequência Conservada , Regulação da Expressão Gênica no Desenvolvimento , Dados de Sequência Molecular , Pirimidinas/análise , Processamento Pós-Transcricional do RNA , Proteínas de Ligação a RNA/biossíntese , Proteínas de Ligação a RNA/química , Sequências Reguladoras de Ácido Nucleico , Proteínas Ribossômicas/biossíntese , Proteínas Ribossômicas/química , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Xenopus laevis/embriologia , Xenopus laevis/metabolismoRESUMO
We have previously constructed and selected six recombinant plasmids containing cDNA sequences specific for different ribosomal proteins of Xenopus laevis (Bozzoni et al., 1981). DNA cloned in these plasmids have been isolated and sequenced. Amino acid sequences of the corresponding portions of the proteins have been derived from DNA sequences; they are arginine- and lysine-rich as expected for ribosomal proteins. One of the cDNA sequences has an open reading frame also on the strand complementary to the one coding for the ribosomal protein; this fragment has inverted repeats twenty nucleotides lone at the two ends. The codon usage for the six sequences appears to be non-random with some differences among the ribosomal proteins analysed.
Assuntos
DNA Recombinante/análise , Proteínas Ribossômicas/genética , Xenopus laevis/genética , Animais , Sequência de Bases , Escherichia coli/genética , Genes , Dados de Sequência Molecular , PlasmídeosRESUMO
The nucleotide sequence of the gene of human ribosomal protein S26 has been assembled from cDNA and genomic PCR-amplified DNA fragments, and its transcription start site has been determined by primer extension. The gene is composed of four exons and three introns spanning 2027bp. Like other ribosomal protein genes of vertebrates, this gene contains a short first exon corresponding exactly to the short untranslated 5'- UTR. Its transcription start site is embedded in a polypyrimidine tract. Using PCR on DNAs from hybrid cell lines with a different set of human chromosomes, the intron-containing gene of ribosomal protein S26 was mapped to human chromosome 12.
Assuntos
Genes/genética , Proteínas Ribossômicas/genética , Proteínas Ribossômicas/isolamento & purificação , Sequência de Bases , Mapeamento Cromossômico , Cromossomos Humanos Par 12/genética , Clonagem Molecular , DNA/química , DNA/genética , DNA/isolamento & purificação , Primers do DNA , Amplificação de Genes , Genes/fisiologia , Genoma , Células HeLa , Humanos , Células Híbridas , Íntrons/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas/genética , Regiões Promotoras Genéticas/fisiologia , Proteínas Ribossômicas/química , Análise de Sequência de DNA , TATA Box/genética , Transcrição Gênica/genéticaRESUMO
We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12,700 bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidine-rich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
Assuntos
Proteínas Ribossômicas/genética , Xenopus laevis/genética , Animais , Sequência de Bases , Southern Blotting , Clonagem Molecular , DNA , Éxons , Íntrons , Dados de Sequência Molecular , Mapeamento por RestriçãoRESUMO
Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of the general translational apparatus can specifically affect the translation of TOP-mRNAs. To verify one of the two hypotheses we induced a partial inhibition of translation initiation in Xenopus cultured cells and analyzed the effect on TOP-mRNA translation. Our results suggest that a specific regulator is necessary to explain the translational control of these of mRNAs.
Assuntos
Polirribossomos/metabolismo , Biossíntese de Proteínas , RNA Mensageiro/química , RNA Mensageiro/metabolismo , Animais , Linhagem Celular , Meios de Cultura Livres de Soro , Regulação da Expressão Gênica , Temperatura Alta , Rim , Cinética , Modelos Genéticos , Pactamicina/farmacologia , Polirribossomos/efeitos dos fármacos , Inibidores da Síntese de Proteínas/farmacologia , Xenopus laevisRESUMO
Gene HRPS26 encoding the S26 human ribosomal protein has been sequenced. Gene HRPS26 consists of four exons and three introns. Its size is 2027 bp; the size of its mRNA is 438 nucleotides. As most of the genes of ribosomal proteins of vertebrates, the HRPS26 gene has a short first exon, which corresponds to the 5'-untranslated region of mRNA; the origin of transcription is located in the polypyrimidine tract. The functional activity of the cloned HRPS26 promoter region has been confirmed by transcription of hybrid plasmids in HeLa cells.